Ivonne Romero, Tania Mandina Cardoso, Mariana Cabitto, Mayra Deminge, Julieta Fernandez Rearte, Hernan Vaquero, Fabiana Farias de Lima, Mariana Esposito Mendes, Laís Melo Silva, Erika Flavia Lafuente-Álvarez, Ana Rada-Tarifa, Valentina Verdejo, Analia Radl, Nicolás Saavedra, Mauricio Santibañez, Nelson Brenes Obando, Fabio Andrés Chaves-Campos, Fernando Ortíz, Luisa Valle, Jorge Ernesto González Mesa, Angela Bastidas, Gabriela Muñoz-Velástegui, Carolina Arceo-Maldonado, Yolanda Citlali Guerrero-Carbajal, Sara Aguilar-Coronel, Norma Monjagata, Marco Espinoza-Zevallos, Wilner Martínez-López, Burix Mechoso, María Vittoria Di Tomaso, Aida Falcón de Vargas, Omar García Lima
{"title":"LBDNet实验室间在高剂量电离辐射下使用双中心加咖啡因测定的比较。","authors":"Ivonne Romero, Tania Mandina Cardoso, Mariana Cabitto, Mayra Deminge, Julieta Fernandez Rearte, Hernan Vaquero, Fabiana Farias de Lima, Mariana Esposito Mendes, Laís Melo Silva, Erika Flavia Lafuente-Álvarez, Ana Rada-Tarifa, Valentina Verdejo, Analia Radl, Nicolás Saavedra, Mauricio Santibañez, Nelson Brenes Obando, Fabio Andrés Chaves-Campos, Fernando Ortíz, Luisa Valle, Jorge Ernesto González Mesa, Angela Bastidas, Gabriela Muñoz-Velástegui, Carolina Arceo-Maldonado, Yolanda Citlali Guerrero-Carbajal, Sara Aguilar-Coronel, Norma Monjagata, Marco Espinoza-Zevallos, Wilner Martínez-López, Burix Mechoso, María Vittoria Di Tomaso, Aida Falcón de Vargas, Omar García Lima","doi":"10.1080/09553002.2025.2494554","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To assess the performance of the LBDNet laboratories in estimating dose over 5 Gy of ionizing radiation using the dicentric chromosome plus caffeine assay.</p><p><strong>Materials and methods: </strong>Dose-response curve fitting: Peripheral blood was irradiated <i>in vitro</i> between 5 and 25 Gy. Then, the DC plus caffeine assay was carried out. Thirteen laboratories received and analyzed metaphase images. The linear dose-response curve was fitted for each laboratory. Dose estimation was performed analyzing coded metaphase images from three different irradiated samples (7.5, 15, 20 Gy) and using the fitted curve from every laboratory.</p><p><strong>Results: </strong>The dose estimation accuracy was within the expected dose ranges. The 76.9%, 84.6% and 69.2% of the estimated doses fell into the ± 20% of the true radiation dose. The 92.3%, 92.3%, and 61.5% of the 95% of the confidence interval of the estimated doses included the true radiation dose. The trueness was 0.9%, 4.4% and 9.6%. The Coefficients of Variation of the estimated doses were 14.5%, 16.1% and 17.8%. Results from only one laboratory were deemed questionable for dose estimation, based on the <i>Z-score</i> derived from robust methods.</p><p><strong>Conclusion: </strong>The intercomparison study yielded satisfactory results; however, dose estimation accuracy tended to decrease, and variability between laboratory results increased as the dose level rose.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"636-651"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"LBDNet inter-laboratory comparison at high doses of ionizing radiation using the dicentric plus caffeine assay.\",\"authors\":\"Ivonne Romero, Tania Mandina Cardoso, Mariana Cabitto, Mayra Deminge, Julieta Fernandez Rearte, Hernan Vaquero, Fabiana Farias de Lima, Mariana Esposito Mendes, Laís Melo Silva, Erika Flavia Lafuente-Álvarez, Ana Rada-Tarifa, Valentina Verdejo, Analia Radl, Nicolás Saavedra, Mauricio Santibañez, Nelson Brenes Obando, Fabio Andrés Chaves-Campos, Fernando Ortíz, Luisa Valle, Jorge Ernesto González Mesa, Angela Bastidas, Gabriela Muñoz-Velástegui, Carolina Arceo-Maldonado, Yolanda Citlali Guerrero-Carbajal, Sara Aguilar-Coronel, Norma Monjagata, Marco Espinoza-Zevallos, Wilner Martínez-López, Burix Mechoso, María Vittoria Di Tomaso, Aida Falcón de Vargas, Omar García Lima\",\"doi\":\"10.1080/09553002.2025.2494554\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To assess the performance of the LBDNet laboratories in estimating dose over 5 Gy of ionizing radiation using the dicentric chromosome plus caffeine assay.</p><p><strong>Materials and methods: </strong>Dose-response curve fitting: Peripheral blood was irradiated <i>in vitro</i> between 5 and 25 Gy. Then, the DC plus caffeine assay was carried out. Thirteen laboratories received and analyzed metaphase images. The linear dose-response curve was fitted for each laboratory. Dose estimation was performed analyzing coded metaphase images from three different irradiated samples (7.5, 15, 20 Gy) and using the fitted curve from every laboratory.</p><p><strong>Results: </strong>The dose estimation accuracy was within the expected dose ranges. The 76.9%, 84.6% and 69.2% of the estimated doses fell into the ± 20% of the true radiation dose. The 92.3%, 92.3%, and 61.5% of the 95% of the confidence interval of the estimated doses included the true radiation dose. The trueness was 0.9%, 4.4% and 9.6%. The Coefficients of Variation of the estimated doses were 14.5%, 16.1% and 17.8%. Results from only one laboratory were deemed questionable for dose estimation, based on the <i>Z-score</i> derived from robust methods.</p><p><strong>Conclusion: </strong>The intercomparison study yielded satisfactory results; however, dose estimation accuracy tended to decrease, and variability between laboratory results increased as the dose level rose.</p>\",\"PeriodicalId\":94057,\"journal\":{\"name\":\"International journal of radiation biology\",\"volume\":\" \",\"pages\":\"636-651\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of radiation biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/09553002.2025.2494554\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of radiation biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/09553002.2025.2494554","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/5 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
LBDNet inter-laboratory comparison at high doses of ionizing radiation using the dicentric plus caffeine assay.
Purpose: To assess the performance of the LBDNet laboratories in estimating dose over 5 Gy of ionizing radiation using the dicentric chromosome plus caffeine assay.
Materials and methods: Dose-response curve fitting: Peripheral blood was irradiated in vitro between 5 and 25 Gy. Then, the DC plus caffeine assay was carried out. Thirteen laboratories received and analyzed metaphase images. The linear dose-response curve was fitted for each laboratory. Dose estimation was performed analyzing coded metaphase images from three different irradiated samples (7.5, 15, 20 Gy) and using the fitted curve from every laboratory.
Results: The dose estimation accuracy was within the expected dose ranges. The 76.9%, 84.6% and 69.2% of the estimated doses fell into the ± 20% of the true radiation dose. The 92.3%, 92.3%, and 61.5% of the 95% of the confidence interval of the estimated doses included the true radiation dose. The trueness was 0.9%, 4.4% and 9.6%. The Coefficients of Variation of the estimated doses were 14.5%, 16.1% and 17.8%. Results from only one laboratory were deemed questionable for dose estimation, based on the Z-score derived from robust methods.
Conclusion: The intercomparison study yielded satisfactory results; however, dose estimation accuracy tended to decrease, and variability between laboratory results increased as the dose level rose.
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