International journal of radiation biology最新文献

{"title":"Collaborative activities in a biological dosimetry network for radiation emergencies in South Korea.","authors":"Yang Hee Lee, Su San Yang, Hyo Jin Yoon, Hwa Young Kim, Soon Woo Kwon, Soo Kyung Jeong, Su Jung Oh, Seong-Hoon Park, Younghyun Lee, Ki Moon Seong","doi":"10.1080/09553002.2024.2447506","DOIUrl":"10.1080/09553002.2024.2447506","url":null,"abstract":"<p><strong>Purpose: </strong>Biological dosimetry is an essential analytic method to estimate the absorbed radiation dose in the human body by measuring changes in biomolecules after radiation exposure. Joint response in a network to mass-casualty radiation incidents is one way to overcome the limitations of biological dosimetry, sharing the workload among laboratories. This study aimed to investigate the current performance, collaborative activities and technical advances of the Korea biodosimetry network (K-BioDos), and suggest the future directions toward successful joint response.</p><p><strong>Materials and methods: </strong>A survey was performed to investigate the capacities of each laboratory and their expectations for the K-BioDos network. We summarized the capacities, expectations and technical advances of K-BioDos members. Based on the results, in-depth discussion was carried out to determine the future plan and activities of K-BioDos.</p><p><strong>Results: </strong>K-BioDos has grown to six laboratories since its establishment with three functional laboratories of biological dosimetry in South Korea. We constructed long-term strategy according the survey results, and performed various activities for enhanced biological dosimetry capabilities - including intercomparison exercises, education, and resource sharing. Through these active collaborations we achieved harmonization of biodosimetry protocols and technical improvement such as better image quality.</p><p><strong>Conclusions: </strong>K-BioDos network performed various activities for joint response and constructed long-term plans, considering the expectations and feedbacks of members. K-BioDos continue to support members to establish and develop biodosimetry tools. These efforts and findings could serve as a fundamental guide for coordinated network responses in the event of large-scale radiological disaster.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"274-282"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manifestation of the FLASH effect in proton irradiation of embryos.","authors":"S V Akulinichev, S I Glukhov, E A Kuznetsova, Yu K Gavrilov, D A Kokontsev, V V Martynova, G V Merzlikin, I A Yakovlev","doi":"10.1080/09553002.2024.2435338","DOIUrl":"10.1080/09553002.2024.2435338","url":null,"abstract":"<p><strong>Purpose: </strong>In order to study the FLASH effect using live models, this work compared proton-induced damage to embryos (nine days after fertilization) and one-day-old chicks (18 days after fertilization) from irradiated at different dose rates eggs of Japanese quail (<i>Coturnix coturnix japónica</i>).</p><p><strong>Materials and methods: </strong>Eggs were irradiated with protons in different modes depending on the dose rate: in a conventional mode (<1 Gy/s, CONV), in a flash mode (∼100 Gy/s, FLASH) and in a single-pulse flash mode (∼10⁵ Gy/s SPLASH).</p><p><strong>Results: </strong>By the criteria of body weight and length, as well as the number of erythrocytes with micronuclei in nine-day-old embryos from eggs irradiated in the spread-out Bragg peak (SOBP) (8.5 Gy), FLASH and SPLASH modes were found to be less traumatic compared with the CONV mode. Among all irradiated embryos, the maximum body weight and length were observed in the SPLASH mode. The lowest death incidence and the smallest number of abnormal erythrocytes were recorded after FLASH and SPLASH irradiation. In chicks that hatched from eggs irradiated in the CONV mode, a tendency for an increase in the number of abnormal erythrocytes was observed. The speed of movement of chicks from FLASH- and SPLASH-irradiated eggs was comparable with that from unirradiated eggs, while chicks from eggs irradiated in the CONV mode were less active than all others.</p><p><strong>Conclusions: </strong>The proton irradiation of eggs in SOBP using high dose-rate modes is less damaging for healthy tissues and for the development of embryos and chicks on the cellular, anatomical, and physiological levels.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"144-152"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An examination of the dose rate effect in mice assuming that the carcinogenic effect of radiation is life shortening resulting from a tissue reaction.","authors":"Nori Nakamura","doi":"10.1080/09553002.2024.2442690","DOIUrl":"10.1080/09553002.2024.2442690","url":null,"abstract":"<p><strong>Purpose: </strong>Radiation exposures do not seem to increase the proportion of mice dying from tumors, but rather cause a shift in the appearance of spontaneous cancers, allowing them to appear earlier, and hence produce a life shortening effect. Then, it was possible to estimate the effect of the dose rate on the carcinogenic effects of radiation using life shortening effects as a measure.</p><p><strong>Conclusion: </strong>The dose response for the induction of life shortening was linear under acute exposure conditions, which indicates that the response under chronic exposure conditions is also likely to be linear, and hence the dose rate factor (DRF) would be constant throughout the dose. Furthermore, the life shortening effect decreased sharply with an increase in age at exposure. To separate the dose rate effect from the effects of age under long-term exposure conditions, a thought experiment was designed which consisted of 8 repeated exposures to an acute 1 Gy dose at intervals of 50 days with an assumption that the effect is additive, and the results were compared with those observed in a chronic continuous exposure experiment (20 mGy per day for 400 days, for a total of 8 Gy: Tanaka et al. 2003). The results showed 211 days of life shortening in the former and 120 days in the latter, which provided a DRF of 1.8 (211/120). If one assumes that a tissue reaction is the primary cause of radiation carcinogenesis, the contrasting two concepts, radiation hormesis and linear-non-threshold model at low doses, would become compatible.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"225-231"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technetium-99m radiolabeling of graphene quantum dots (GQDs) as a new probe for glioblastoma tumor imaging.","authors":"Maryam Mazaheri Tehrani, Mostafa Erfani, Mojtaba Amiri, Mostafa Goudarzi","doi":"10.1080/09553002.2024.2404460","DOIUrl":"10.1080/09553002.2024.2404460","url":null,"abstract":"<p><strong>Purpose: </strong>Cancer diagnosis involves a multi-step process. Accurate identification of the tumor, staging and development of cancer cells is crucial for selecting optimal treatments to minimize disease recurrence. Quantum dots (QDs) represent an exciting class of fluorescent nanoprobes in molecular detection and targeted tumor imaging.</p><p><strong>Materials and methods: </strong>In this study, graphene quantum dots (GQDs) were synthesized by pyrolysis of citric acid (CA) as a carbon precursor under high temperatures. The morphology of the obtained GQDs was first characterized using physical (TEM and DLS) and spectroscopic (fluorescence, FTIR and UV-Vis) methods. In the following,^99mTc-labeled GQDs were prepared in the presence of SnCl₂.2H₂O as a reducing agent between 95 and 100 °C. The biodistribution and tumor targeting efficiency of radiolabeled GQDs as a novel agent for C6 glioma tumor scintigraphy in an animal model were evaluated. Furthermore, organ uptake, human serum albumin binding and tumor accumulation were measured.</p><p><strong>Results: </strong>The TEM image of the prepared GQDs showed a relatively uniform size distribution in the range of diameter 6-9 nm and spherical shape. Radiolabeled GQDs showed a radiochemical yield of >97% (<i>n</i> = 3). Through incubation in human serum, almost 15% of ^99mTc-labeled GQDs degraded after 6 h. The amount of uptake in xenograft models of glioma C6 rats was 1.10 ± 0.36% of injection dose per gram after 1 h. The kidneys, intestinal and glioma tumor sites were observed via scintigraphy imaging.</p><p><strong>Conclusion: </strong>Our data suggest that ^99mTc-labeled GQDs, as a new radiotracer, efficiently accumulate in the tumor site and could be included as a radiotracer for detecting glioma tumors.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"65-72"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"γ-radiation induced reduction in antinutrients of buckwheat (<i>Fagopryum esculentum</i> Moench) seeds and leaves.","authors":"Kuldip Chandra Verma, Kumkum Giri, Sanjay Kumar Verma, Pawanesh Tamta, Nidhi Joshi","doi":"10.1080/09553002.2024.2445580","DOIUrl":"10.1080/09553002.2024.2445580","url":null,"abstract":"<p><strong>Purpose: </strong>Buckwheat, a dicotyledonous crop of Polygonaceae family, is known for its nutritional value and adaptability to adverse climates. Local people reported that prolonged consumption of buckwheat seeds and leaves causes numbness and gastrointestinal problems. The present study was conducted to observe the impact of different doses of γ-radiations on phytoconstituents of buckwheat seeds and leaves, to make them nutritionally superior.</p><p><strong>Materials and methods: </strong>Buckwheat seeds were treated with 5, 10, 15 and 20 kGy doses of γ-radiations and grown in an experimental farm. Various phytoconstituents in seeds and leaves were analyzed.</p><p><strong>Results: </strong>The antioxidant, phenol, flavonoid, β-carotene, iron, calcium, lysine and arginine were increased significantly (<5%) with increasing doses of γ-radiations up to 10 kGy, whereas, anti-nutrients (tannin, phytic acid and oxalate) decreased significantly (<5%). γ-radiation @ 10 kGy is the best for the enhancement of phytoconstituents in buckwheat seeds from a nutrition point of view. Phytoconstituents in buckwheat leaves and irradiated seed progeny were positively co-related with M1 seeds.</p><p><strong>Conclusions: </strong>It can be concluded that the buckwheat seeds treated with a 10 kGy dose of γ-radiation are the best to produce green leaves as hara saag, and progeny seeds for preparation of flour. However, superior mutant selection and effect of by-products from γ-irradiated buckwheat seeds is the thrust area of future research.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"314-326"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FASN inhibition shows the potential for enhancing radiotherapy outcomes by targeting glycolysis, AKT, and ERK pathways in breast cancer.","authors":"Ching-I Chen, Deng-Yu Kuo, Hui-Yen Chuang","doi":"10.1080/09553002.2024.2446585","DOIUrl":"10.1080/09553002.2024.2446585","url":null,"abstract":"<p><strong>Purpose: </strong>Breast cancer ranks as the most prevalent cancer in women, characterized by heightened fatty acid synthesis and glycolytic activity. Fatty acid synthase (FASN) is prominently expressed in breast cancer cells, regulating fatty acid synthesis, thereby enhancing tumor growth and migration, and leading to radioresistance. This study aims to investigate how FASN inhibition affects cell proliferation, migration, and radioresistance in breast cancer, as well as the mechanisms involved.</p><p><strong>Materials and methods: </strong>We used lentiviruses carrying sh<i>FASN</i> to create FASN-knockdown cell lines called MCF-7-sh<i>FASN</i> and MDA-MB-231-sh<i>FASN</i>. We conducted Western blot analysis to determine the expression levels of FASN and other proteins of interest. Furthermore, we evaluated cellular glucose uptake and migration using the ¹⁸F-FDG assay, wound healing, and transwell assays. We also employed the MTT assay to assess the short-term survival of the negative control and FASN-knockdown cells after irradiation.</p><p><strong>Results: </strong>FASN knockdown led to a decrease in the expressions of proteins related to fatty acid synthesis and glycolysis in both MCF-7-sh<i>FASN</i> and MDA-MB-231-sh<i>FASN</i> cells when compared to their counterparts. Moreover, reduced ¹⁸F-FDG uptake and lactate production were also detected after FASN knockdown. FASN knockdown inhibited cell proliferation and survival by downregulating the AKT, ERK, and AMPK pathways and promoted apoptosis by increasing the BAX/p-Bcl-2 ratio. In addition, FASN knockdown impaired cell migration while enhancing radiosensitivity.</p><p><strong>Conclusions: </strong>FASN knockdown disrupts fatty acid synthesis and glycolysis, inhibits cell proliferation and induces apoptosis. The increased radiosensitivity after FASN inhibition suggests that it could potentially complement radiotherapy in treating breast cancer.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"292-303"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial uncoupler 2,4-dinitrophenol (DNP) confers protection from the acute effect of ionizing radiation by regulating redox homeostasis in radio-sensitive organs of C57BL/6 mice.","authors":"Yogesh Rai, Saurabh Singh, Dhananjay K Sah, Ankit Chauhan, Neeraj Kumari, Rakesh Pandey, Kumudini Paliwal, Ankit Choudhary, Anant Narayan Bhatt","doi":"10.1080/09553002.2025.2462077","DOIUrl":"10.1080/09553002.2025.2462077","url":null,"abstract":"<p><strong>Purpose: </strong>The development of radiation countermeasures is an unmet need. Several cytoprotective approaches have been demonstrated to minimize ionizing radiation-induced tissue toxicity. Previously, our research indicated that mild mitochondrial uncoupling by 2,4-dinitrophenol (DNP) potentially counteract the IR-induced cell death. Herein, our investigation delves into the radio-protective efficacy of DNP following total-body irradiation (TBI) in C57BL/6 mice.</p><p><strong>Method: </strong>DNP was administered orally 2 h prior to TBI (7.6 Gy gamma (γ)-radiation) as a prophylactic approach against acute radiation syndrome (ARS), and survival analysis was performed for 30 days in C57BL/6 mice. Radiation-induced reactive oxygen species (ROS) and macromolecular oxidation (lipid and protein) were examined in radiation-sensitive organs, including the spleen, bone marrow, liver, and gastrointestinal tract (GI). GI damage was examined using tissue histology and correlated with the level of antioxidant catalase, superoxide dismutase, and reduced glutathione (GSH).</p><p><strong>Results: </strong>DNP conferred radioprotection in mice, as evidenced by a 41% increase in survival. DNP pretreatment facilitates a substantial regulation of TBI-induced ROS production and macromolecular oxidation as early as 30 min to 24 h post-irradiation across various radiation-sensitive organs. Moreover, antioxidant analysis in GI tissue showed that DNP played a crucial role in maintaining redox homeostasis. This was evident through the elevation of catalase, superoxide dismutase, and GSH, eventually protecting from radiation-induced GI damage.</p><p><strong>Conclusion: </strong>The study confirms that DNP-mediated regulation of redox homeostasis in the hematopoietic and GI systems enhances survival efficacy while mitigating the risk of acute tissue damage and provides protection against ARS in C57BL/6 mice.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"358-369"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichostatin A mitigates acute and late effects of radiation in intestine by regulation of DNA damage repair and Wnt/TGFβ/Smad signaling.","authors":"Akshu Dahiya, Aliza Rehan, Paban K Agrawala, Ajaswrata Dutta","doi":"10.1080/09553002.2024.2430250","DOIUrl":"10.1080/09553002.2024.2430250","url":null,"abstract":"<p><strong>Purpose: </strong>Radiation accidents and misuse of nuclear weapons elevate the risk of development of acute life-threatening injuries as well as their late effects are noted in survivors. Currently, no countermeasure agents are available for the management of radiation-induced GI injury (RIGI) in humans. In the present study, the radiomitigative potential of Trichostatin A (TSA) was evaluated against acute and late RIGI.</p><p><strong>Methods: </strong>15 Gy gamma radiation was delivered to the whole abdomen of C57BL/6 mice, followed by intravenous TSA (150 ng/kg) administration after 1 h and 24 h. Acute changes were checked 24 h and 3.5 days post irradiation. Mice were monitored for development of fibrosis, survival for 1 year and alteration in different signaling pathways.</p><p><strong>Result: </strong>15 Gy abdominal irradiation activated the DNA damage marker (γ-H2AX) by nearly 3.2 ± 0.29 fold and regulated the repair proteins, XRCC1 and PARP1 in the intestine, which was differentially regulated by TSA. The Wnt signaling pathway and stem cell proliferation in the intestine were also positively regulated by TSA. The TSA administered mice demonstrated improved intestinal morphology. 12.5% of TSA administered mice survived upto 1 year whereas 100% of 15 Gy exposed mice died by 6 months. The surviving mice that had received TSA showed reduced intestinal fibrosis than 15 Gy group, possibly via downregulation of TGFβ/Smad signaling.</p><p><strong>Conclusion: </strong>The findings suggest that TSA have the potential to mitigate both acute and late effects of radiation in the intestine and can be explored as promising agent in the management of RIGI.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"15-27"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin as a radioprotective agent against flattening filter and flattening filter-free beam in radiotherapy-induced lung tissue damage.","authors":"Zuhal Özer Simsek, Serhat Aras, Makbule Cikrikcioglu, Kursad Nuri Baydili, Mustafa Cortuk","doi":"10.1080/09553002.2024.2381492","DOIUrl":"10.1080/09553002.2024.2381492","url":null,"abstract":"<p><strong>Background: </strong>Radiotherapy is a widely used treatment method in oncology, applied by delivering high-energy particles or waves to the tumor tissue. Although tumor cells are targeted with radiotherapy, it can cause acute or long-term damage to healthy tissues. Therefore, the preservation of healthy tissues has been an important subject of various scientific researches. Melatonin has been shown to have a radioprotective effect on many tissues and organs such as liver, parotid gland, brain, and testicles. This study aimed to evaluate the protective effect of melatonin against the radiation at various doses and rates administered to the lung tissue of healthy mice.</p><p><strong>Methods: </strong>This study was a randomized case-control study conducted with 80 rats comprising 10 groups with eight animals per group. Of the 10 groups, first is the control group, which is not given any melatonin, and second is the group that does not receive RT, which is given only melatonin, and the other eight groups are RT groups, four with melatonin and four without melatonin.</p><p><strong>Results: </strong>There was no statistical difference in terms of histopathological findings in the lung tissue between the second group, which did not receive radiotherapy and received only melatonin, and the control group. Lung damage due to radiotherapy was statistically significantly higher in the groups that did not receive melatonin compared to the groups that received melatonin.</p><p><strong>Conclusions: </strong>This study revealed that melatonin has a protective effect against the cytotoxic damage of RT in rats receiving RT.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"28-34"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141794415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of statistical methods on lower limits of dose estimation in biological dosimetry.","authors":"David Endesfelder, Martin Bucher, Elizabeth A Ainsbury, Ursula Oestreicher","doi":"10.1080/09553002.2024.2440870","DOIUrl":"10.1080/09553002.2024.2440870","url":null,"abstract":"<p><strong>Purpose: </strong>In cases of radiological or nuclear events, biological dosimetry enables decisions whether an individual was exposed to ionizing radiation and the estimation of the dose. Several statistical methods are used to assess uncertainties. The stringency of the applied method has an impact on the lowest dose that can be detected. To obtain reliable and comparable results, it is crucial to harmonize the applied statistical methods.</p><p><strong>Materials and methods: </strong>The decision threshold and detection limit of the statistical methods were derived for variable cell numbers. The coverage of the 95% confidence intervals as well as the false-positive and false-negative rates of the methods were compared based on simulations. The evaluated methods included a graphical method, the propagation of errors and a Bayesian method.</p><p><strong>Results: </strong>The minimum resolvable doses, the doses at the detection limit and the coverage were relatively variable between the compared methods. The Bayesian method showed the best coverage, lowest resolvable doses and had false-positive rates close to 5%. The graphical method with the combination of two 83% confidence intervals also showed promising results. The other methods were either too conservative or underestimated the uncertainties for some doses or cell numbers.</p><p><strong>Conclusions: </strong>The assessment of the lower dose limits is a central part of biological dosimetry and the applied statistical methods have a strong influence on the interpretation of the results. Simulations enable comparisons between methods and provide important information for the harmonization and standardization of the uncertainty assessment.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"107-117"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}