International journal of laboratory hematology最新文献_第5页

Clinical Applications of Bone Marrow CD34 Immunohistochemistry (BM CD34 IHC) Assay: International Council for Standardization in Hematology (ICSH) Guidelines. 骨髓CD34免疫组化（BM CD34 IHC）检测的临床应用：国际血液学标准化委员会（ICSH）指南。

International journal of laboratory hematology Pub Date : 2024-12-21 DOI: 10.1111/ijlh.14411

Emina Emilia Torlakovic, Katherine R Calvo, Tracy George, Elizabeth Hyjek, Szu-Hee Lee, Anna Porwit, Elena Sabattini, Leonie Saft, Xiaoge Zhou, Alexandar Tzankov

{"title":"Clinical Applications of Bone Marrow CD34 Immunohistochemistry (BM CD34 IHC) Assay: International Council for Standardization in Hematology (ICSH) Guidelines.","authors":"Emina Emilia Torlakovic, Katherine R Calvo, Tracy George, Elizabeth Hyjek, Szu-Hee Lee, Anna Porwit, Elena Sabattini, Leonie Saft, Xiaoge Zhou, Alexandar Tzankov","doi":"10.1111/ijlh.14411","DOIUrl":"https://doi.org/10.1111/ijlh.14411","url":null,"abstract":"Introduction: Investigation of bone marrow (BM) trephine biopsies and/or clot sections by CD34 immunohistochemistry (IHC) testing has been used by pathologists for several decades, and its clinical value has been well established with QBEND10 being the most frequently used primary antibody (Ab) clone. However, most other parameters related to the IHC protocol as well as the readout vary widely between clinical laboratories and in the published literature. The ICSH Working Group having reviewed the published evidence has established guidelines that will help to harmonize performance and reporting of CD34 IHC on BM biopsies.Methods: The methodology is based on the published \"guidelines for guidelines\" (GRADE, AGREE) with modifications to the specific laboratory medicine environment and to specific key questions. Review of the published literature resulted in 49 articles relevant to clinical applications of the BM CD34 IHC. Five key questions were addressed including testing indications, technical performance, readout methodology, terminology, and reporting.Results: A total of 23 guidelines were grouped according to the key questions.Conclusion: BM CD34 IHC testing is complex with both the protocol and the pathologist's readout requiring validation to ensure reported results are reproducible and accurate. The readout/interpretation must be adapted to a specific purpose of the assay (clinicopathological question) and the type and overall sample quality. Standardized terminology and reporting are essential if CD34 IHC assay is being used for clinical diagnostics.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concurrent Loss of CD16 in Granulocytes and CD14 in Mature Monocytes in a Patient With Pancytopenia: A Diagnostic Clue for Paroxysmal Nocturnal Hemoglobinuria. 全血细胞减少症患者粒细胞 CD16 和成熟单核细胞 CD14 同时丢失：阵发性夜间血红蛋白尿症的诊断线索。

International journal of laboratory hematology Pub Date : 2024-12-14 DOI: 10.1111/ijlh.14417

Abhishek Prasad, Eric D Carlsen, Sergio Pina-Oviedo, Luis F Carrillo

引用次数: 0

Post-Induction Bone Marrow Necrosis With Carbapenem-Resistant Enterobacteriaceae in a Case of B-Acute Lymphoblastic Leukemia. b急性淋巴细胞白血病诱导后骨髓坏死伴碳青霉烯耐药肠杆菌科

International journal of laboratory hematology Pub Date : 2024-12-04 DOI: 10.1111/ijlh.14407

Prerna Arora, Deepika Yadav, Jasmita Dass, Mukul Aggarwal, Vishwanath Singh Yadav, Arti Kapil

引用次数: 0

Relapse of Monomorphic Epitheliotropic Intestinal T-Cell Lymphoma (MEITL) in a Pericardial Fluid. 心包积液中的单形上皮细胞性肠T细胞淋巴瘤（MEITL）复发。

International journal of laboratory hematology Pub Date : 2024-11-17 DOI: 10.1111/ijlh.14398

Elsa Bera, Liana Veresezan, Maïssa Souissi, Fanny Drieux, Pierre Lebreton, Victor Bobée

引用次数: 0

Atypical Melanocytic Cells in the Cerebrospinal Fluid of a 7-Year-Old Child With Headaches. 一名 7 岁头痛儿童脑脊液中的非典型黑色素细胞

International journal of laboratory hematology Pub Date : 2024-11-07 DOI: 10.1111/ijlh.14399

Weijie Li, Suzanne Schauwecker, Trevor Gerson

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Cytomorphological Evaluation in a Pediatric Case of Metastatic Neuroblastoma: A Challenging Diagnosis due to Unusual Differentiation. 对一例小儿转移性神经母细胞瘤的细胞形态学评估：因不寻常的分化而极具挑战性的诊断。

International journal of laboratory hematology Pub Date : 2024-10-22 DOI: 10.1111/ijlh.14388

K Truijens, C Van Aerde, T Tousseyn, M Tajdar

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An Unusual Presentation of EATL. 不寻常的 EATL 演示。

International journal of laboratory hematology Pub Date : 2024-10-21 DOI: 10.1111/ijlh.14389

Radu Chiriac, Lucile Baseggio

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Acute Mast Cell Leukemia With Complex Karyomorphism and Karyotypes. 具有复杂核形态和核型的急性肥大细胞白血病

International journal of laboratory hematology Pub Date : 2024-10-16 DOI: 10.1111/ijlh.14384

Sha Li, Yantian Zhao

{"title":"Acute Mast Cell Leukemia With Complex Karyomorphism and Karyotypes.","authors":"Sha Li, Yantian Zhao","doi":"10.1111/ijlh.14384","DOIUrl":"https://doi.org/10.1111/ijlh.14384","url":null,"abstract":"A 51-year-old woman visited the emergency department because of intermittent fever for more than 2 weeks. Physical examination found symmetrical pitting edema of the extremities. Computed tomography and ultrasound showed multiple serous effusions and splenomegaly. A complete blood cell analysis showed moderate anemia and severe thrombocytopenia. Abnormal cells in a subsequent peripheral blood smear accounted for 5%. The bone marrow smear showed a large number of abnormal cells (69.5%), with large cell bodies and pseudopod protrusions. Huge multinucleated cells were visible. Nucleoli were visible in some nuclei, and mitotic figures were evident. The abnormal cells by peroxidase staining were negative, and the positive rate by periodic acid-Schiff staining was 62%. Bone marrow biopsy revealed diffuse infiltration of heterotypic cells (approximately 80%). Later, she was transferred to the hematology department. The phenotypes by flow cytometry were CD117bri+, CD30+, MPO-, CD2-, and CD25-. The molecular test for KIT gene mutation was negative, and multiple karyotypes were abnormal and complex. The final diagnosis was mast cell leukemia. The patient eventually died approximately 1 month after the diagnosis.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute Myeloid Leukaemia With Morphologic and Immunophenotypic Differentiation to Acute Erythroid Leukaemia at the Time of Relapse. 复发时形态学和免疫分型分化为急性红细胞白血病的急性髓性白血病

International journal of laboratory hematology Pub Date : 2024-10-15 DOI: 10.1111/ijlh.14386

Katie Liston, Vitaliy Mykytiv

引用次数: 0

Chronic Myeloid Leukaemia (CML) in T-Lymphoblastic Blast Crisis With Near Early T Cell Progenitor (ETP) Immunophenotype. T淋巴细胞突变危象中的慢性髓性白血病（CML），免疫表型接近早期T细胞祖细胞（ETP）。

International journal of laboratory hematology Pub Date : 2024-10-11 DOI: 10.1111/ijlh.14383

Ke Xu, Evan Vistsaras, Rajeev Gupta

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