Cell Population Data Parameters in Dengue—Is There Any Significance? A Single-Center Study

IF 2.2 4区医学 Q3 HEMATOLOGY

International Journal of Laboratory Hematology Pub Date : 2025-02-04 DOI:10.1111/ijlh.14434

Jawad Hassan, Sarah Javed, Shakir Ahmed, Waqas Javeed, Madiha Abid

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引用次数: 0

Abstract

Objective

To assess the significance of cell population data parameters (CPD) in dengue positive individuals.

Methodology

A retrospective cross-sectional study was conducted at the National Institute of Blood Disease and Bone Marrow Transplantation (NIBD and BMT), Karachi, Pakistan from July 2022 to September 2022 (in a period of 3 months of peak dengue fever outbreak). Dengue fever is a viral infection that is transmitted from mosquitoes to humans. It is more prevalent in tropical and subtropical environments. A total of 389 individuals, who presented with febrile illness at the NIBD clinics, were screened for dengue and malaria with Complete Blood Count (CBC), Dengue nonstructural protein 1 (NS1) antigen test and Malaria Parasite Immunochromatographic test (MP-ICT). Whole blood samples were collected and analyzed for CBC on Sysmex XN hematology analyzers. All 65 CPD and standard CBC parameters were analyzed. Statistical analysis was performed using SPSS version 25.0. Descriptive analysis of all the parameters was performed and a p value < 0.001 was considered significant. Positive and negative correlation was also evaluated within the parameters to assess their significance. Furthermore, cut-off values of CPD parameters were evaluated plotting their receiver operating characteristic (ROC) curves.

Results

Out of the 389 febrile patients, 137 were diagnosed as dengue-positive. Descriptive analysis for mean and median values of parameters revealed statistically significant difference for seven parameters (namely WBC, PLT-F, NEUT, LYMP, MONO, HFLC, and LY-WY) in the comparison of the two groups which were then further assessed for positive and negative correlation. Multivariate logistic regression analysis revealed High Fluorescence Lymphocyte Count (HFLC) to be the distinguishing parameter among dengue positive and negative cases. Compared to all the CPD parameters of our data set, the area under curve for lymphocytes cell size and the width of dispersion (LY-WZ) displayed a borderline value of 0.582.

Conclusion

Sysmex XN hematology analyzers can provide extensive information about CPD parameters, allowing for the prompt differentiation among febrile illnesses and dengue infection. HFLC and other significant parameters demonstrate promise as rapid, adjunctive diagnostic tools. Further research is needed to validate these findings and optimize the clinical utility of CPD parameters in dengue management.

查看原文本刊更多论文

登革热的细胞群体数据参数-有什么意义吗？单中心研究。

目的：探讨细胞群数据参数（CPD）在登革热阳性人群中的意义。方法：于2022年7月至2022年9月（在登革热疫情高峰期的3个月期间）在巴基斯坦卡拉奇国家血液病和骨髓移植研究所（NIBD和BMT）进行了一项回顾性横断面研究。登革热是一种由蚊子传播给人类的病毒感染。它在热带和亚热带环境中更为普遍。通过全血细胞计数（CBC）、登革非结构蛋白1 （NS1）抗原检测和疟原虫免疫层析检测（MP-ICT）对在NIBD诊所出现发热性疾病的389例个体进行了登革热和疟疾筛查。采集全血样本，在Sysmex XN血液学分析仪上检测CBC。分析65例CPD和标准CBC参数。采用SPSS 25.0版本进行统计分析。结果：389例发热患者中，有137例诊断为登革热阳性。描述性分析参数的平均值和中位数显示，两组比较WBC、PLT-F、NEUT、LYMP、MONO、HFLC、LY-WY等7个参数差异有统计学意义，进一步评估其正相关和负相关。多因素logistic回归分析显示，高荧光淋巴细胞计数（HFLC）是登革热阳性和阴性病例的区分参数。与我们数据集的所有CPD参数相比，淋巴细胞大小和弥散宽度（LY-WZ）的曲线下面积显示出0.582的边缘值。结论：Sysmex XN血液学分析仪可提供广泛的CPD参数信息，可快速区分发热性疾病和登革热感染。HFLC和其他重要参数显示出作为快速辅助诊断工具的前景。需要进一步的研究来验证这些发现，并优化CPD参数在登革热管理中的临床应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.