International Journal of Laboratory Hematology最新文献

{"title":"Breaking Free From MCHC Interferences? French-Speaking Cellular Haematology Group (GFHC) Review of Causes, Rising Trends and Practical Solutions","authors":"Sandrine Girard,&nbsp;Yaël Berda-Haddad,&nbsp;Chantal Brouzes,&nbsp;Bouchra Badaoui,&nbsp;Agathe Boussaroque,&nbsp;Alexandre Janel,&nbsp;Bernard Chatelain,&nbsp;Véronique Baccini","doi":"10.1111/ijlh.14536","DOIUrl":"10.1111/ijlh.14536","url":null,"abstract":"<p>Mean corpuscular haemoglobin concentration (MCHC) is determined by the ratio of haemoglobin concentration to haematocrit. Managing increased MCHC presents significant challenges, mainly due to variations in analytical methods and pathophysiological conditions. Depending on the haematological analyser (HA), MCHC can be measured directly or calculated. It is important that all people involved in hematocytometry must identify and correct artefacts to ensure accurate erythrocyte parameters. In order to harmonise and standardise haematology practices in all laboratories, the French-speaking Cellular Haematology Group (GFHC) has reviewed the interferences and pathophysiological situations that could increase MCHC, and the advice on how to manage cases of elevated MCHC. We will review current techniques, such as impedance and optical methods, for accurate determination of MCHC. We will also examine the interferences that can artificially increase MCHC; and the pathophysiological conditions responsible for such increases. Finally, we will present guidelines for the management of elevated MCHC, including strategies to bypass interferences and determine which erythrocyte parameters can be reliably reported, as well as the acceptable MCHC values for various pathophysiological variations.</p>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"798-807"},"PeriodicalIF":2.3,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Molecular Landscape of MPO in Kikuchi's Disease: Protein Expression, mRNA Levels, and Genetic Polymorphisms","authors":"Chen Chang,&nbsp;MengNa Zhang,&nbsp;Yu Chang,&nbsp;Yu Ma,&nbsp;Wensheng Li","doi":"10.1111/ijlh.14509","DOIUrl":"10.1111/ijlh.14509","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>This study examines the protein expression and mRNA levels of myeloperoxidase (MPO) in patients with Kikuchi's disease, and explores the association between the MPO-463G/A polymorphism and Kikuchi's disease.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Paraffin blocks from 43 patients with Kikuchi's disease were collected, and paraffin blocks from patients with reactive hyperplastic lymph nodes, granulomatous inflammation, and myeloid sarcoma were used as controls. Immunohistochemistry (IHC), quantitative reverse transcription polymerase chain Reaction (qRT-PCR) and Sanger sequencing were used to detect the relevant variants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The positive rate of MPO protein expression in the Kikuchi's disease group was 100% by IHC. In comparison to the control group, patients with Kikuchi's disease exhibited elevated <i>MPO</i> mRNA expression levels, which demonstrated a positive correlation with protein expression levels. Kikuchi's disease and reactive hyperplastic lymph nodes displayed distinct genotypes at the MPO-463 locus, with mutation phenotypes of 7% and 30%, respectively. The G allele at this locus emerged as a risk factor.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In Kikuchi's disease, both the protein and mRNA expression levels of MPO are elevated, and the high expression of mRNA is positively correlated with the protein expression levels. The polymorphism at the MPO-463 locus may be associated with the occurrence of Kikuchi's disease.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"898-905"},"PeriodicalIF":2.3,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concurrent Cryptococcus neoformans and Hematoidin in a HIV-Negative Male Patient on Peripheral Blood Smears","authors":"Hong Li,&nbsp;Ningning Zhao,&nbsp;Ting Li,&nbsp;Ying Bu,&nbsp;Hui Wang","doi":"10.1111/ijlh.14528","DOIUrl":"10.1111/ijlh.14528","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"796-797"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostically Challenging but Infrequent Aberrant Immunophenotypic Patterns in B-Lymphoblastic Leukemia/Lymphoma (B-ALL)","authors":"Kritika Krishnamurthy,&nbsp;Joseph Sweeney,&nbsp;Qing Wang,&nbsp;Yanan Fang,&nbsp;Yang Shi,&nbsp;Yanhua Wang","doi":"10.1111/ijlh.14522","DOIUrl":"10.1111/ijlh.14522","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"997-1002"},"PeriodicalIF":2.3,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Laboratories Need More Information About Commercially Available Reagents to Prepare for the IVDR: A Call From the ICSH","authors":"Dominique Lasne,&nbsp;Sophie Testa,&nbsp;Steve Kitchen,&nbsp;Chris Gardiner,&nbsp;Piet Meijer,&nbsp;François Mullier","doi":"10.1111/ijlh.14506","DOIUrl":"10.1111/ijlh.14506","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>According to the new In Vitro Diagnostic Medical Device Regulation (EU) (IVDR) an In Vitro Medical Device (IVD) is considered as a laboratory developed test (LDT) if used outside of intended use. It is therefore essential that the information given by the manufacturer about the intended use is clear, precise, and well documented. For now, the only source of information for laboratories is the instructions for use (IFU). Our primary aim was to analyze the IFU provided by several manufacturers for a large panel of coagulation assays. The secondary objective was to provide a list of minimum information that must be accessible to clinical laboratories.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We analyzed 195 IFU for the main assays used in European hemostasis clinical laboratories commercialized by 12 manufacturers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The “intended use” section appears in almost all IFU, but the information given in this section is very heterogeneous. We observed disagreement for each of the assays assessed with some intended uses not supported by guidance or guidelines. Some indications in use by clinical laboratories are not provided by the manufacturers. We only found information on clinical performance for a limited number of assays. For some assays, data are available in the literature but are not reported in the IFU. The matrix and the importance of a pre-test probability are not systematically mentioned.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We urgently request access to the necessary information to know the intended use of a reagent according to the IVDR. We define the minimum information that should be available to laboratories. We call for joint discussions to maintain innovation and ensure the quality, safety, and accessibility of innovative diagnostics.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"938-988"},"PeriodicalIF":2.3,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14506","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate Predictor for Successful Collection of Peripheral Blood Stem Cells in Patients With Multiple Myeloma and Lymphoma","authors":"Hongyan Li,&nbsp;Can Yan","doi":"10.1111/ijlh.14517","DOIUrl":"10.1111/ijlh.14517","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"995-996"},"PeriodicalIF":2.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144478275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flow Cytometric Assessment of TRBC1 Expression for the Diagnosis of T-Cell Lymphoma: Clinical Utility and Pitfalls Related to T-Cell Clones of Uncertain Significance","authors":"SooHo Yu,&nbsp;Boram Kim,&nbsp;Sang Eun Yoon,&nbsp;Hee-Jin Kim,&nbsp;Hyun-Young Kim","doi":"10.1111/ijlh.14508","DOIUrl":"10.1111/ijlh.14508","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>T-cell receptor constant beta chain 1 (TRBC1) has emerged as a potential clonal marker for T-cell lymphoma. This study evaluated TRBC1 expression using flow cytometry in healthy individuals and various clinical specimens, assessing its clinical utility in the diagnosis of T-cell lymphomas.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Flow cytometric analysis was performed on peripheral blood specimens from 20 healthy individuals and 186 clinical specimens (116 bone marrow aspirates, 18 peripheral bloods, 52 body fluids) from 177 patients for the exclusion or differential diagnosis of T-cell lymphoma using a panel of nine monoclonal antibodies: CD45, CD3, CD4, CD8, CD56, CD2, CD5, CD7, and TRBC1.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>TRBC1 showed polytypic expression in healthy individuals with a median of 38.6% (range, 33.9%–46.1%) in CD4+ T cells and 26.3% (range, 10.9%–44.2%) in CD8+ T cells (<i>p &lt;</i> 0.001). TRBC1 expression in non-T-cell lymphoma specimens was comparable to that in healthy peripheral blood, except for bone marrow, which showed slightly higher TRBC1 expression. All 20 T-cell lymphoma cases exhibited monotypic TRBC1 expression, with a predominance of TRBC1− (65%). Additionally, T-cell clones of uncertain significance (TCUS) were detected in 12.7% of non-T-cell lymphoma specimens, predominantly in CD8+ T cells and associated with cytopenic and reactive conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study demonstrates the clinical utility of flow cytometric TRBC1 assessment in diagnosing T-cell lymphomas, characterizing TRBC1 expression patterns, and identifying TCUS in a large cohort of specimens without T-cell lymphoma involvement across diverse clinical settings. Given the relatively frequent detection of TCUS, these findings underscore the importance of interpreting TRBC1 flow cytometry results within the appropriate clinical context.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"840-848"},"PeriodicalIF":2.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14508","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of WHO-HAEM5 Revisions on the Morphological Interpretation of CLL","authors":"Fiamma Balboni,&nbsp;Antonio La Gioia,&nbsp;Fabiana Fiorini,&nbsp;Francesca Di Nezza,&nbsp;Miriam Marsano,&nbsp;Cecilia Vitali,&nbsp;Diego La Gioia,&nbsp;Benedetta Romboli","doi":"10.1111/ijlh.14510","DOIUrl":"10.1111/ijlh.14510","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"992-994"},"PeriodicalIF":2.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Single-Center Retrospective Study of the Investigation of Monoclonal B-Cell Lymphocytosis (MBL) and Chronic Lymphocytic Leukemia (CLL) in Southwestern Ontario: Are We Over-Investigating?","authors":"Ortenc Hoxha,&nbsp;Mrinal Lad,&nbsp;Benjamin D. Hedley,&nbsp;Ian H. Chin-Yee,&nbsp;Cyrus C. Hsia,&nbsp;Benjamin Chin-Yee","doi":"10.1111/ijlh.14507","DOIUrl":"10.1111/ijlh.14507","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Monoclonal B-cell lymphocytosis (MBL) is a common cause of lymphocytosis in older individuals. Although cytogenetic/molecular testing is usually reserved for patients requiring treatment, recent studies suggest that early testing may have a prognostic benefit in MBL and early-stage chronic lymphocytic leukemia (CLL). We evaluated local practices of expanded diagnostics in the MBL/CLL population to assess (1) adherence to international workshop on CLL (iwCLL) guidelines with respect to additional cytogenetic testing and (2) anticipated costs of expanded diagnostic testing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A retrospective chart review was conducted on all patients who underwent flow cytometry testing at our center for a suspected hematologic disorder between 2016 and 2021. Patients were subdivided into CLL, high-count MBL, and low-count MBL, and cytogenetic/molecular testing numbers were calculated as well as associated costs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of 974 patients who underwent flow cytometry testing, 100 had CLL, 49 had high-count MBL, and 5 had low-count MBL. Cytogenetic testing was performed in 54/100 CLL, 2/49 high-count MBL, and 0/5 low-count MBL patients. Most testing occurred in symptomatic CLL patients (38/54) especially after the 2018 iwCLL guideline changes. The estimated cost of cytogenetic testing for the 56 patients tested was $27 600. Expanding testing to all patients would have incurred an additional $87 900 during the study period.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This retrospective study shows high adherence to iwCLL guidelines for the investigation of early-stage CLL/MBL, especially after the 2018 guideline changes. Future studies are needed to weigh the benefits of improved prognostication against resources/costs required by expanded cytogenetics/molecular testing in these common hematologic conditions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"891-897"},"PeriodicalIF":2.3,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Massive Cryptococcosis in Peripheral Blood Smear","authors":"Alba Puyuelo,&nbsp;Cristina Fernández-Maqueda,&nbsp;Laura Benitez-Gutierrez,&nbsp;Isabel Sánchez-Romero,&nbsp;Rafael Forés","doi":"10.1111/ijlh.14511","DOIUrl":"10.1111/ijlh.14511","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"794-795"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}