Chen Chang, MengNa Zhang, Yu Chang, Yu Ma, Wensheng Li
{"title":"揭示菊地病中MPO的分子景观：蛋白质表达，mRNA水平和遗传多态性。","authors":"Chen Chang, MengNa Zhang, Yu Chang, Yu Ma, Wensheng Li","doi":"10.1111/ijlh.14509","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Purpose</h3>\n \n <p>This study examines the protein expression and mRNA levels of myeloperoxidase (MPO) in patients with Kikuchi's disease, and explores the association between the MPO-463G/A polymorphism and Kikuchi's disease.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Paraffin blocks from 43 patients with Kikuchi's disease were collected, and paraffin blocks from patients with reactive hyperplastic lymph nodes, granulomatous inflammation, and myeloid sarcoma were used as controls. Immunohistochemistry (IHC), quantitative reverse transcription polymerase chain Reaction (qRT-PCR) and Sanger sequencing were used to detect the relevant variants.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The positive rate of MPO protein expression in the Kikuchi's disease group was 100% by IHC. In comparison to the control group, patients with Kikuchi's disease exhibited elevated <i>MPO</i> mRNA expression levels, which demonstrated a positive correlation with protein expression levels. Kikuchi's disease and reactive hyperplastic lymph nodes displayed distinct genotypes at the MPO-463 locus, with mutation phenotypes of 7% and 30%, respectively. The G allele at this locus emerged as a risk factor.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>In Kikuchi's disease, both the protein and mRNA expression levels of MPO are elevated, and the high expression of mRNA is positively correlated with the protein expression levels. The polymorphism at the MPO-463 locus may be associated with the occurrence of Kikuchi's disease.</p>\n </section>\n </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"898-905"},"PeriodicalIF":2.3000,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14509","citationCount":"0","resultStr":"{\"title\":\"Unveiling the Molecular Landscape of MPO in Kikuchi's Disease: Protein Expression, mRNA Levels, and Genetic Polymorphisms\",\"authors\":\"Chen Chang, MengNa Zhang, Yu Chang, Yu Ma, Wensheng Li\",\"doi\":\"10.1111/ijlh.14509\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Purpose</h3>\\n \\n <p>This study examines the protein expression and mRNA levels of myeloperoxidase (MPO) in patients with Kikuchi's disease, and explores the association between the MPO-463G/A polymorphism and Kikuchi's disease.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Paraffin blocks from 43 patients with Kikuchi's disease were collected, and paraffin blocks from patients with reactive hyperplastic lymph nodes, granulomatous inflammation, and myeloid sarcoma were used as controls. Immunohistochemistry (IHC), quantitative reverse transcription polymerase chain Reaction (qRT-PCR) and Sanger sequencing were used to detect the relevant variants.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The positive rate of MPO protein expression in the Kikuchi's disease group was 100% by IHC. In comparison to the control group, patients with Kikuchi's disease exhibited elevated <i>MPO</i> mRNA expression levels, which demonstrated a positive correlation with protein expression levels. Kikuchi's disease and reactive hyperplastic lymph nodes displayed distinct genotypes at the MPO-463 locus, with mutation phenotypes of 7% and 30%, respectively. The G allele at this locus emerged as a risk factor.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>In Kikuchi's disease, both the protein and mRNA expression levels of MPO are elevated, and the high expression of mRNA is positively correlated with the protein expression levels. 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Unveiling the Molecular Landscape of MPO in Kikuchi's Disease: Protein Expression, mRNA Levels, and Genetic Polymorphisms
Purpose
This study examines the protein expression and mRNA levels of myeloperoxidase (MPO) in patients with Kikuchi's disease, and explores the association between the MPO-463G/A polymorphism and Kikuchi's disease.
Methods
Paraffin blocks from 43 patients with Kikuchi's disease were collected, and paraffin blocks from patients with reactive hyperplastic lymph nodes, granulomatous inflammation, and myeloid sarcoma were used as controls. Immunohistochemistry (IHC), quantitative reverse transcription polymerase chain Reaction (qRT-PCR) and Sanger sequencing were used to detect the relevant variants.
Results
The positive rate of MPO protein expression in the Kikuchi's disease group was 100% by IHC. In comparison to the control group, patients with Kikuchi's disease exhibited elevated MPO mRNA expression levels, which demonstrated a positive correlation with protein expression levels. Kikuchi's disease and reactive hyperplastic lymph nodes displayed distinct genotypes at the MPO-463 locus, with mutation phenotypes of 7% and 30%, respectively. The G allele at this locus emerged as a risk factor.
Conclusions
In Kikuchi's disease, both the protein and mRNA expression levels of MPO are elevated, and the high expression of mRNA is positively correlated with the protein expression levels. The polymorphism at the MPO-463 locus may be associated with the occurrence of Kikuchi's disease.
期刊介绍：
The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology.
The journal publishes invited reviews, full length original articles, and correspondence.
The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines.
The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.
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