International journal of laboratory hematology最新文献_第4页

Myeloid sarcoma of the breast with synchronous early T-cell precursor acute lymphoblastic leukemia: A rare presentation. 乳腺髓样肉瘤伴同步早期T细胞前体急性淋巴细胞白血病：罕见病例。

International journal of laboratory hematology Pub Date : 2024-08-19 DOI: 10.1111/ijlh.14360

Subhajit Hajra, Kavya Ur, Zahed Ali Qamer, Karthik Kumar, Priyavadhana Balasubramanian, Gaurav Dhingra, Neha Singh, Nilotpal Chowdhury

引用次数: 0

An automated method for thrombocyte counting in capillary microsamples. 毛细管微型样本中血小板计数的自动方法。

International journal of laboratory hematology Pub Date : 2024-08-14 DOI: 10.1111/ijlh.14354

Caroline Vasard Boesen, Vibeke Staun Christensen, Klaus Rosenkilde Jensen, Anja Reinert Hansen, Claus Vinter Bødker Hviid

{"title":"An automated method for thrombocyte counting in capillary microsamples.","authors":"Caroline Vasard Boesen, Vibeke Staun Christensen, Klaus Rosenkilde Jensen, Anja Reinert Hansen, Claus Vinter Bødker Hviid","doi":"10.1111/ijlh.14354","DOIUrl":"https://doi.org/10.1111/ijlh.14354","url":null,"abstract":"Introduction: We aimed to develop an automated, low-volume method for thrombocyte counting in capillary blood using the Sysmex predilution (PD) mode.Methods: Microsamples were prepared by resuspension of 50 μL blood in 300 μL DCL CellPack. Thrombocyte counting was done in the impedance (PLT-I) and fluorescence (PLT-F) channels. The imprecision and bias was evaluated in >394 microsamples from adult blood. Preanalytical factors (skin-piercing, storage, and transportation in our pneumatic tube system) was assessed, and studies on pediatric microsamples were made for comparison. The improvement in analytical quality and turnaround time was examined.Results: For PLT-F, the imprecision was 1.1%-3.7%, and the bias was 10.1% (95% CI: 8.8-11.3). After skin-piercing, the bias was 8.1% (95% CI: 5.6-10.6) and the imprecision 1.9% (95% CI: 1.3-2.5). Thrombocyte counts kept stable after 4 h at room temperature (94.8% [95% CI: 93.2-96.4]) and after pneumatic tube transportation [6.7% (95% CI: 4.8-8.6)]. The bias of the PD mode for pediatric microsamples was 13.0% (95% CI: -8.4-34.4) in the PLT-F channel. The automated method had a considerably lower imprecision than the existing manual thrombocyte counting method and reduced turnaround times.Conclusion: The automated microsample method offers a low-volume alternative for measurement of thrombocytes. The method appears useful also in pediatric samples.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rare case of bone marrow infiltration by Langerhans cell with bilobed and folded lobulated nuclei. 一个罕见的朗格汉斯细胞骨髓浸润病例，其细胞核呈双叶和折叠分叶状。

International journal of laboratory hematology Pub Date : 2024-08-14 DOI: 10.1111/ijlh.14362

Jinshuang Bo, Shuai Wang, Shenghai Gao, Xiaowen Xin

引用次数: 0

Mature plasmacytoid dendritic cell proliferation associated with acute myeloid leukemia. 与急性髓性白血病相关的成熟浆细胞树突状细胞增殖。

International journal of laboratory hematology Pub Date : 2024-08-10 DOI: 10.1111/ijlh.14356

J Maes, H Devos, B Cauwelier, P De Paepe, J Emmerechts

引用次数: 0

Anaplastic large cell lymphoma with ATIC::ALK in leukemic phase and associated with hemophagocytic lymphohistiocytosis. 白血病期患有 ATIC::ALK 并伴有嗜血细胞淋巴组织细胞增多症的无性大细胞淋巴瘤。

International journal of laboratory hematology Pub Date : 2024-08-07 DOI: 10.1111/ijlh.14355

Shuyu E, Guilin Tang, Shaoying Li, Ellen Schlette, Sa A Wang, Jie Xu

引用次数: 0

Establishing reflex test rules for platelet fluorescent counting method using machine learning models on Sysmex XN-series hematology analyzer. 在 Sysmex XN 系列血液分析仪上使用机器学习模型建立血小板荧光计数法的反射测试规则。

International journal of laboratory hematology Pub Date : 2024-08-05 DOI: 10.1111/ijlh.14353

Zhengyu Zhou, Mengqiao Guo, Kang Wu, Zhanyi Yue

{"title":"Establishing reflex test rules for platelet fluorescent counting method using machine learning models on Sysmex XN-series hematology analyzer.","authors":"Zhengyu Zhou, Mengqiao Guo, Kang Wu, Zhanyi Yue","doi":"10.1111/ijlh.14353","DOIUrl":"https://doi.org/10.1111/ijlh.14353","url":null,"abstract":"Introduction: The platelet fluorescent counting (PLT-F) method is utilized as a reflex test method following the initial test of the platelet impedance counting (PLT-I) method in clinical practice on the Sysmex XN-series automated hematology analyzer. Our aim is to establish reflex test rules for the PLT-F method by combining multiple parameters provided by the \"CBC + DIFF\" mode of the Sysmex XN-series automated hematology analyzer.Methods: We tested 120 samples to evaluate the baseline bias between the PLT-F and PLT-I methods. Then, we selected 1256 samples to establish and test reflex test rules using seven machine learning models (decision Tree, random forest, neural network, logistic regression, k-nearest neighbor, support vector machine, and Naive Bayes). The training set and test set were divided at a ratio of 7:3. We evaluated the performance of machine learning models on the test set using various metrics to select the most valuable model.Results: The PLT-F method exhibited a high degree of correlation with the PLT-I method (r = 0.998). The random forest model emerged as the most valuable, boasting an accuracy of 0.893, an area under the curve of 0.954, an F1 score of 0.771, a recall of 0.719, a precision of 0.831, and a specificity of 0.950. The most important variable in the random forest model was mean cell volume, weighted at 15.09%.Conclusion: The random forest model, which demonstrated high efficiency in our study, can be used to establish PLT reflex test rules based on the PLT-F method for the Sysmex XN-series automated hematology analyzer.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of an integrated activated partial thromboplastin time (Cephen LS/Cephen) for the detection of lupus anticoagulant. 评估用于检测狼疮抗凝物的综合活化部分凝血活酶时间（Cephen LS/Cephen）。

International journal of laboratory hematology Pub Date : 2024-08-05 DOI: 10.1111/ijlh.14349

Adrien Thiriet, Vincent Poindron, Laurent Sattler, Jordan Wimmer, Delphine Rolland, Anne-Sophie Korganow, Laurent Mauvieux, Agathe Herb

{"title":"Evaluation of an integrated activated partial thromboplastin time (Cephen LS/Cephen) for the detection of lupus anticoagulant.","authors":"Adrien Thiriet, Vincent Poindron, Laurent Sattler, Jordan Wimmer, Delphine Rolland, Anne-Sophie Korganow, Laurent Mauvieux, Agathe Herb","doi":"10.1111/ijlh.14349","DOIUrl":"https://doi.org/10.1111/ijlh.14349","url":null,"abstract":"Introduction: It is recommended to use two chronometric assays of different principles for the diagnosis of lupus anticoagulant (LA), consisting in diluted Russell Viper Venom Time (dRVVT) and activated Partial Thromboplastin Time (aPTT). Yet, there are only a few integrated aPTT assays; this study aims to evaluate one of them: Cephen LS/Cephen (Hyphen Biomed).Method: 249 samples of patients were included in this study. Normal reference ranges were determined with platelet-poor plasma (PPP) from healthy blood donors. Performances were then evaluated by comparing Cephen LS/Cephen test results to the results of the laboratory's reference assay for the diagnosis of LA and to clinical data, both on non-anticoagulated and anticoagulated patients' samples (Unfractioned heparin (UFH), Low Molecular Weight Heparin (LMWH), Vitamin K Antagonists (VKA) and apixaban). Interference of UFH, LMWH and VKA were also evaluated thanks to spiking experiment of increasing heparin concentrations or factor deficiency.Results: Cephen LS/Cephen test had 48.6% sensitivity towards LA. Although UFH and VKA seemed to interfere with this assay and were likely to cause false negative, LMWH and apixaban did not. Finally, combination of Cephen LS/ Cephen with dRVVT had 89.0% sensitivity.Conclusion: Cephen LS/Cephen seems relevant for LA diagnosis, in combination with dRVVT, and might be used in patients undergoing LMWH or apixaban therapy.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population-level anemia prevalence rates may be rendered inaccurate when hemoglobin is measured in pooled capillary blood or with the HemoCue® 301 device. 如果使用毛细管集合血液或 HemoCue® 301 设备测量血红蛋白，人群贫血患病率可能会不准确。

International journal of laboratory hematology Pub Date : 2024-07-30 DOI: 10.1111/ijlh.14342

Kelsey M Cochrane, Brock A Williams, Hou Kroeun, Am Chanthan, Crystal D Karakochuk

引用次数: 0

Combined peripheral blood monocyte count and white blood cell count as a guide for successful one-day autologous peripheral blood stem cell collection. 将外周血单核细胞计数和白细胞计数相结合，作为一天成功采集自体外周血干细胞的指南。

International journal of laboratory hematology Pub Date : 2024-07-29 DOI: 10.1111/ijlh.14351

Phandee Watanaboonyongcharoen, Nattarat Lorucharoen, Kitsada Wudhikarn, Udomsak Bunworasate, Chantiya Chanswangphuwana, Ponlapat Rojnuckarin

{"title":"Combined peripheral blood monocyte count and white blood cell count as a guide for successful one-day autologous peripheral blood stem cell collection.","authors":"Phandee Watanaboonyongcharoen, Nattarat Lorucharoen, Kitsada Wudhikarn, Udomsak Bunworasate, Chantiya Chanswangphuwana, Ponlapat Rojnuckarin","doi":"10.1111/ijlh.14351","DOIUrl":"https://doi.org/10.1111/ijlh.14351","url":null,"abstract":"Introduction: Peripheral blood stem cells (PBSCs) are the most common source of stem cell transplantation, which depends on an adequate number of CD34+ cells. Although pre-apheresis CD34+ cell count is a standard guide for the collection, it is not always available. This study aimed to evaluate complete blood count parameters for predicting successful one-day autologous PBSC collection.Methods: Data from the patients who underwent autologous PBSC collection at a tertiary care hospital were retrospectively reviewed.Results: There were 123 patients (185 leukapheresis procedures). Successful PBSC collection (CD34+ cells ≥4.0 × 106 cells/kg) was obtained in 85 patients (69.1%), of which 55 (44.7%) were successfully obtained on the first day. The median CD34+ collection efficiency was 44.1%. The mean platelet loss during apheresis was 39.9%. The adverse event rate was 18.9%. Patients in whom PBSCs were collected within one day were less likely to experience adverse effects related to leukapheresis. Pre-apheresis CD34+ cells ≥10 cells/μLand combined white blood cell (WBC) counts ≥5 × 109/L and/or monocyte ≥10% were independently associated with the successful one-day PBSC collection (adjusted odds ratio 24.06, 95% confidence interval [CI] 5.30-109.10, p < 0.001; and 6.94, 95% CI 1.35-35.79, p = 0.021, respectively). Only pre-apheresis CD34+ cells had a strong correlation with the total stem cell yield.Conclusions: To reduce the complication of leukapheresis, the combined pre-apheresis WBC ≥5 × 109/L and/or monocyte ≥10% is a practical parameter to initiate a successfully one-day PBSC collection with or without pre-apheresis CD34+ cell results.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141794412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transient neutrophil morphological abnormalities in a post-chemotherapy patient receiving G-CSF. 化疗后接受 G-CSF 治疗的患者出现短暂的中性粒细胞形态异常。

International journal of laboratory hematology Pub Date : 2024-07-29 DOI: 10.1111/ijlh.14352

Yangyang Tan, Xinjian Cai

引用次数: 0