International journal of laboratory hematology最新文献_第4页

Hematopathology Practice in the Digital Era: What has Changed? 数字时代的血液病实践：发生了什么变化？

International journal of laboratory hematology Pub Date : 2025-08-14 DOI: 10.1111/ijlh.14515

Olga Pozdnyakova

引用次数: 0

Nuclear Budding in Peripheral Blood Smear of Nodal Marginal Zone Lymphoma. 淋巴结边缘区淋巴瘤外周血涂片中核出芽。

International journal of laboratory hematology Pub Date : 2025-08-14 DOI: 10.1111/ijlh.14543

Radu Chiriac

引用次数: 0

Evaluation of International Council for Standardization in Haematology Recommendations on Activated Partial Thromboplastin Time Mixing Tests Using an Automated Haemostasis Analyser. 国际血液学标准化委员会对使用自动止血分析仪的活化部分凝血活素时间混合试验建议的评价。

International journal of laboratory hematology Pub Date : 2025-08-05 DOI: 10.1111/ijlh.14537

Shapla Kamali, Minal Dave, Priyanka Raheja, Suthesh Sivapalaratnam, Sean Platton

{"title":"Evaluation of International Council for Standardization in Haematology Recommendations on Activated Partial Thromboplastin Time Mixing Tests Using an Automated Haemostasis Analyser.","authors":"Shapla Kamali, Minal Dave, Priyanka Raheja, Suthesh Sivapalaratnam, Sean Platton","doi":"10.1111/ijlh.14537","DOIUrl":"https://doi.org/10.1111/ijlh.14537","url":null,"abstract":"Introduction: Plasma mixing tests are frequently performed in haemostasis laboratories to aid in the determination of the cause of prothrombin time or activated partial thromboplastin time (APTT) prolongation. The International Council for Standardization in Haematology (ICSH) has recently published recommendations for performing and interpreting mixing test; we evaluated the ICSH recommendations for APTT mixing tests on patient samples using automated mixing on a Sysmex CN-series analyser.Methods: Samples from patients with haemophilia A with and without inhibitors, or patients with positive lupus anticoagulant, or patients on rivaroxaban/edoxaban, with an APTT ≥ 4 s above normal, were tested using five different APTT reagents: Siemens Actin FS, Actin FSL, and Pathromtin SL; and Hyphen Biomed Cephen and Cephen-LS.Results: A likely/possible inhibitor was erroneously diagnosed in all haemophilia patients when assessed using the ICSH criteria, except with Actin FS (erroneous diagnosis in 95%). Using CN-series parameters with locally-derived reference ranges, ≤ 15% of haemophilia patients were erroneously diagnosed. Only Cephen-LS reliably detected lupus anticoagulant by any algorithm.Conclusions: When using lupus-insensitive reagents, APTT-mixing tests are of limited value in discriminating between factor deficiencies, lupus anticoagulants, or inhibitors. Incubated mixing tests are essential when diagnosing a FVIII-inhibitor. Rather than perform mixing tests, it is better to be guided by clinical presentation and perform further investigations as appropriate, including analysis of anti-Xa activity for the presence of a direct factor-Xa inhibiting anticoagulant, factor assays in patients with bleeding (or with suspected acquired haemophilia A), and lupus anticoagulant assays in patients with no bleeding.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144791043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute Leukemia Warning Model Combined CBC and CPD Data Based on Machine Learning. 基于机器学习的综合CBC和CPD数据的急性白血病预警模型。

International journal of laboratory hematology Pub Date : 2025-08-05 DOI: 10.1111/ijlh.14538

Hong-Wei Gao, Ying-Ying Wang, Xiang Li, Zhen-Hua Liu, Jiang-Ying Cai, Wan-Xia Yang, Fang-Fang Wang, Zhi-Peng Sun, Chong-Ge You

{"title":"Acute Leukemia Warning Model Combined CBC and CPD Data Based on Machine Learning.","authors":"Hong-Wei Gao, Ying-Ying Wang, Xiang Li, Zhen-Hua Liu, Jiang-Ying Cai, Wan-Xia Yang, Fang-Fang Wang, Zhi-Peng Sun, Chong-Ge You","doi":"10.1111/ijlh.14538","DOIUrl":"https://doi.org/10.1111/ijlh.14538","url":null,"abstract":"Background: Early diagnosis plays a crucial role in improving the survival rate of acute leukemia (AL) patients. This study aims to develop a warning model for the detection of acute leukemia (AL) using complete blood count (CBC) and cell population data (CPD), which could aid in clinical diagnosis.Methods: In this study, CBC and CPD were utilized to develop a warning model for assisting clinical diagnosis of AL. Clinical characteristics and peripheral blood data were retrospectively collected from 262 AL patients and 280 non-AL patients at the Second Hospital of Lanzhou University; they were randomly divided into a training set and a test set in a ratio of 7:3. The training set was used to establish support vector machine (SVM), random forest (RF), and logistic regression (LR) models for AL. The validation set consisted of 357 cases (97 AL, 260 non-AL) collected from the General Hospital of Ningxia Medical University to verify the warning efficacy of the optimal model in conjunction with the test set.Results: The comparative analysis revealed that the SVM model outperformed the RF and LR models in terms of diagnostic accuracy. In the training set, the accuracy was 92.93%; the area under the ROC curve (AUC) and 95% confidence interval (95% CI) were 0.981 (0.970, 0.992). For the test set, the accuracy was 89.66%; the AUC and 95% CI were 0.959 (0.931, 0.988). As for the validation set, the accuracy was 76.34%; the AUC and 95% CI were 0.841 (0.789, 0.893). Additionally, the calibration curve and decision curve analysis (DCA) demonstrated that the SVM model exhibited satisfactory effectiveness and feasibility.Conclusion: The SVM model shows significant potential as a clinical screening tool for AL.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144791042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rare Morphology of Abnormal Lymphocytes in Splenic B-Cell Lymphoma/Leukemia With Prominent Nucleoli. 核仁突出的脾b细胞淋巴瘤/白血病异常淋巴细胞的罕见形态。

International journal of laboratory hematology Pub Date : 2025-07-31 DOI: 10.1111/ijlh.14535

Melissa Albesano, Thea Bensi, Stefania Binello, Raffaella Doglio, Tina Ruggiero

引用次数: 0

Translation of the Morphological Hallmarks of Dyserythropoiesis to Objective Morphometric Parameters by Imaging Flow Cytometry. 利用成像流式细胞术将红细胞生成的形态学特征转化为客观形态学参数。

International journal of laboratory hematology Pub Date : 2025-07-26 DOI: 10.1111/ijlh.14534

D V Despoina Violidaki, O A Olof Axler, L N Lars Nilsson, A P Anna Porwit, M E Mats Ehinger

{"title":"Translation of the Morphological Hallmarks of Dyserythropoiesis to Objective Morphometric Parameters by Imaging Flow Cytometry.","authors":"D V Despoina Violidaki, O A Olof Axler, L N Lars Nilsson, A P Anna Porwit, M E Mats Ehinger","doi":"10.1111/ijlh.14534","DOIUrl":"https://doi.org/10.1111/ijlh.14534","url":null,"abstract":"Introduction: Imaging flow cytometry (IFC) is a unique method combining multiparameter flow cytometry (MFC) with morphological evaluation of single cells. Since both analyses are integrated in the diagnostic work-up of myelodysplastic neoplasms (MDS), we wanted to explore the possibilities of IFC as a diagnostic tool for MDS, with focus on dyserythropoiesis.Methods: We analysed fresh bone marrow (BM) aspirates from 26 patients with untreated MDS and MDS/MPN and compared them with 12 normal BM specimens (NBM) exploring the cytoplasmic compartment, nuclear abnormalities, and megaloblastoid changes.Results: The cytoplasmic compartment in MDS showed higher contrast and variance values compared to NBM (p < 0.001). Cells with abnormal nuclei and binucleated forms were significantly increased in MDS compared to NBM (p < 0.05 for both features). Most binucleated forms were found in the mature compartment, and many of them were G1 phase arrested. All maturation stages showed a significant increase in cell size in MDS compared to NBM (p < 0.001). In addition, we found decreased nuclear condensation combined with increased cell size for all erythropoietic maturation stages in MDS compared to NBM (p < 0.001). Finally, our previously described MDS-specific aberrant population of mature erythroblasts with decreased expression of CD36 and/or CD71 showed denser chromatin than both the mature erythropoietic MDS cells without immunophenotypic aberrancies (p < 0.001) and NBM (p = 0.024).Conclusion: IFC can detect the major morphological changes associated with dyserythropoiesis in MDS, including the novel findings of increased cytoplasmic texture and bilobated non-proliferating erythroblasts, allowing for objectivity and standardization.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144736403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital Pathology in Hematopathology: From Vision to Deployment. 血液病中的数字病理学：从视觉到部署。

International journal of laboratory hematology Pub Date : 2025-07-23 DOI: 10.1111/ijlh.14533

Ryan C Shean, Anton V Rets

{"title":"Digital Pathology in Hematopathology: From Vision to Deployment.","authors":"Ryan C Shean, Anton V Rets","doi":"10.1111/ijlh.14533","DOIUrl":"https://doi.org/10.1111/ijlh.14533","url":null,"abstract":"Digital pathology (DP) has evolved alongside other technical advances, transforming our daily lives and diagnostic medicine. It is likely that, as in other areas of life, science, and medicine, the overall level of digitization will continue to rise, along with an increasing number of groups implementing DP. This review explores the clinical DP ecosystem with a focus on hematology and hematopathology, addressing the benefits of DP, such as improved workflow efficiency, remote practice, enhanced collaboration, and integration of artificial intelligence tools. Several challenges and pitfalls are also highlighted, such as technical scanner challenges, image management system issues, IT infrastructure, regulations, and the critical (and expensive) topic of data storage, and retrieval for DP. We also propose a roadmap for the successful implementation of DP, designed to support institutions of all sizes to make the transition to DP. This roadmap emphasizes well-thought-out, strategic planning and aims to ensure that organizations and individuals considering a switch to DP are able to deliver meaningful benefits to pathologists, health systems, providers, and most importantly, patients.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Application of Artificial Intelligence-Based Bone Marrow Cell Analysis System in Pediatric Hematological Diseases. 基于人工智能的骨髓细胞分析系统在小儿血液病中的应用

International journal of laboratory hematology Pub Date : 2025-07-16 DOI: 10.1111/ijlh.14527

Xin He, Fei He, Yan Wang, Yu Liu, Xiaopeng Gao, Mingrui Yu, Haiyan Gao

{"title":"The Application of Artificial Intelligence-Based Bone Marrow Cell Analysis System in Pediatric Hematological Diseases.","authors":"Xin He, Fei He, Yan Wang, Yu Liu, Xiaopeng Gao, Mingrui Yu, Haiyan Gao","doi":"10.1111/ijlh.14527","DOIUrl":"https://doi.org/10.1111/ijlh.14527","url":null,"abstract":"Introduction: The clinical diagnosis of hematological diseases depends on the differential count of nucleated cells on the bone marrow (BM) smears, and an artificial intelligence (AI)-based system was applied to automatically classify BM nucleated cells in pediatric hematological disease samples in this study.Methods: The BM aspirate smears were collected from 213 pediatric patients (under 18 years old) at Harbin Medical University Affiliated Sixth Hospital from October 2023 to June 2024. The entire smear of BM was scanned by a ×40 objective lens to obtain complete digital images using an automated analysis method named Morphogo. Next, Morphogo was used to capture nucleated cells in an area of BM smears that was selected by hematopathologists, with a magnification of ×100 objective lens.Results: Morphogo demonstrated a high overall accuracy (> 87.8%) in pre-classifying nucleated cells in BM aspirate smears obtained from masked pediatric patients. In addition, the average values of sensitivity and accuracy in Morphogo cell classification were remarkably high. Moreover, Morphogo could reduce the time costs on classifying BM nucleated cells. Besides, there were positive correlations between Morphogo and manual categorization for immunologic thrombocytopenic purpura, BM failure, hyperplastic anemia, acute leukemia, chronic myeloid leukemia, and other hematological diseases.Conclusion: This research demonstrated the clinical potential of the Morphogo in early screening of pediatric hematological diseases and its reliability as an automated tool for differential counting and analysis of BM nucleated cells.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Common Hematologic Emergencies-Acute Promyelocytic Leukemia and Microangiopathic Hemolytic Anemias-A Pivotal Role of Clinical Laboratory. 常见血液学急症——急性早幼粒细胞白血病和微血管病溶血性贫血——临床实验室的关键作用。

International journal of laboratory hematology Pub Date : 2025-07-16 DOI: 10.1111/ijlh.14531

Ganna Shestakova, Nicolas Ulrich Edgar, Anton V Rets

引用次数: 0

The Characteristics and Outcomes of Adult Acute Myeloid Leukemia Patients With KMT2A-Partial Tandem Duplication. kmt2a部分串联重复的成人急性髓系白血病患者的特点和预后。

International journal of laboratory hematology Pub Date : 2025-07-15 DOI: 10.1111/ijlh.14532

Dai-Hong Xie, Wen-Min Chen, Yue Hao, Xu Wang, Ling-Di Li, Jin-Ying Li, Zhao-Yu Li, Hao Jiang, Qian Jiang, Xiao-Jun Huang, Ya-Zhen Qin

{"title":"The Characteristics and Outcomes of Adult Acute Myeloid Leukemia Patients With KMT2A-Partial Tandem Duplication.","authors":"Dai-Hong Xie, Wen-Min Chen, Yue Hao, Xu Wang, Ling-Di Li, Jin-Ying Li, Zhao-Yu Li, Hao Jiang, Qian Jiang, Xiao-Jun Huang, Ya-Zhen Qin","doi":"10.1111/ijlh.14532","DOIUrl":"https://doi.org/10.1111/ijlh.14532","url":null,"abstract":"Introduction: KMT2A-Partial Tandem Duplication (KMT2A-PTD) is a recurrent gene mutation present in acute myeloid leukemia (AML) and its prognostic significance needs to be clarified.Methods: Three hundred and eighty-seven consecutive adult newly diagnosed AML patients with non-favorable cytogenetic risk were tested for KMT2A-PTD by real-time quantitative PCR. All patients were screened for AML-related gene fusions and mutations.Results: Thirty-two (8.3%) patients were identified as KMT2A-PTD (+). KMT2A-PTD significantly co-occurred with FLT3-ITD, RUNX1, and DNMT3A mutation and tended to be related to normal karyotype (p < 0.0001, p = 0.0001, 0.019, and 0.062). Furthermore, none of the KMT2A-PTD (+) patients had NPM1 mutation, CEBPA bZIP in-frame mutation (p = 0.0005 and 0.0009), and none of them had KMT2A-rearrangement and other gene fusions (p = 0.16). As a result, all KMT2A-PTD (+) patients were categorized into ELN2022-intermediate or adverse groups (p < 0.0001). KMT2A-PTD was not related to patients' age, sex, white blood cell (WBC) counts, hemoglobin (Hb) level, platelet (PLT) counts, percentage of bone marrow blast cells, and FAB subtypes (all p > 0.05). KMT2A-PTD had no effect on complete remission achievement after 1 and 2 courses of induction therapy, relapse-free survival, and overall survival in both the entire cohort and within the following five subgroups: FLT3-ITD (+), RUNX1 mutation, DNMT3A mutation, ELN2022-intermediate, and ELN2022-adverse categories, respectively (all p > 0.05). Moreover, KMT2A-PTD (+) patients also could not be stratified by them (all p > 0.05).Conclusion: KMT2A-PTD harbored its distinct genetic characteristics and had no prognostic impacts in AML.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0