International journal of laboratory hematology最新文献_第3页

Biological Reference Ranges for Healthy Indian Blood Donors: Experience of a Tertiary Care Center Vis-à-Vis International Literature. 印度健康献血者的生物参考范围：一家三级医疗中心的经验与国际文献的对比。

International journal of laboratory hematology Pub Date : 2024-10-04 DOI: 10.1111/ijlh.14375

Shalini Goel, Aseem Kumar Tiwari, Pawan Kumar Gahlot, Manish Kumar Singh, Renu Saxena, Vaibhav Jadhav, Monisha Sethi, Tan Swee Jin

{"title":"Biological Reference Ranges for Healthy Indian Blood Donors: Experience of a Tertiary Care Center Vis-à-Vis International Literature.","authors":"Shalini Goel, Aseem Kumar Tiwari, Pawan Kumar Gahlot, Manish Kumar Singh, Renu Saxena, Vaibhav Jadhav, Monisha Sethi, Tan Swee Jin","doi":"10.1111/ijlh.14375","DOIUrl":"https://doi.org/10.1111/ijlh.14375","url":null,"abstract":"Introduction: Complete blood count is the most common, basic test requisitioned in hematology. The normal reference ranges of hematological parameters are required owing to variable socioeconomic, environmental, and genetic factors in populations. The current study determines the reference ranges of the healthy Indian donor population of a high socioeconomic group.Methods: The study was conducted in the Department of Transfusion Medicine at a tertiary care hospital in India and included 4098 individuals, aged 18-65 years coming for voluntary blood donation from July 2021 to October 2022. Blood samples were collected in K2EDTA, analyzed on the Sysmex XN-31 hematology analyzer, and using statistical tools, the normal reference ranges were calculated.Results: The reference ranges for hemoglobin (HB) (137-185 g/L), WBC (5.1-1.7 × 109/L), platelet count (115.6-370.0 × 109/L) were noted. No statistically significant changes were observed in different age groups. There were gender-wise differences noted in nearly all parameters. The HB and hematocrit (HCT) range was slightly higher in other Indian and other Asian populations with comparable values with the Chinese, Korean populations, and Western populations; RBC parameters were overall comparable with minor differences; the WBC count was higher than the other Indian and Asian populations particularly the upper limit of lymphocyte and monocyte; and the range of platelet counts had a comparable upper limit with all populations and had the lowest lower value in males in our study, which was comparable to only the Chinese population.Conclusions: It is concluded that reference ranges of common parameters were calculated with minor changes noted in all hematological parameters on comparing with other Indian, Asian population, and Western data.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Gene Expression Microarray for the Classification of Ph-Like B-Cell Acute Lymphoblastic Leukemia. 基因表达微阵列在Ph-Like B细胞急性淋巴细胞白血病分类中的应用

International journal of laboratory hematology Pub Date : 2024-10-02 DOI: 10.1111/ijlh.14370

Nonthaya Thangrua, Teerapong Siriboonpiputtana, Budsaba Rerkamnuaychoke, Takol Chareonsirisuthigul, Veerawat Korkiatsakul, Pongpak Pongphitcha, Ekchol Mukda, Somchai Chutipongtanate, Samart Pakakasama

{"title":"Application of Gene Expression Microarray for the Classification of Ph-Like B-Cell Acute Lymphoblastic Leukemia.","authors":"Nonthaya Thangrua, Teerapong Siriboonpiputtana, Budsaba Rerkamnuaychoke, Takol Chareonsirisuthigul, Veerawat Korkiatsakul, Pongpak Pongphitcha, Ekchol Mukda, Somchai Chutipongtanate, Samart Pakakasama","doi":"10.1111/ijlh.14370","DOIUrl":"https://doi.org/10.1111/ijlh.14370","url":null,"abstract":"Introduction: Ph-like ALL has gene expression profile similar to Ph-positive ALL but without the BCR::ABL1 fusion. The disease presents higher rates of severe clinical features and is associated with unfavorable outcomes. There is still no standard pipeline for molecular characterization of the disease, and no valid predictor gene panel is available worldwide.Methods: We performed expression microarray on 25 B-cell ALL and 6 Ph-positive B-cell ALL to cluster and identify the transcriptional signature of Ph-like ALL. qRT-PCR was used to confirm the expression of candidate genes.Results: Four out of 25 samples (16%) shared gene expression signatures related to and clustered with control Ph-positive samples. Analysis of genes differentially expressed in Ph-like B-cell ALL and evidentially functional in normal blood cell development and leukemogenesis, we selected genes as potential biomarkers for Ph-like B-cell ALL in our dataset: ADGRE2, CD9, EPHA7, FAM129C, TCL1A, and VPREB1. Those genes were filtered by Ph-like gene signatures obtained from distinct reliable data, resulting in five genes, CA6, CHN2, JAK1, JCHAIN, and PON2, selected for validation by qRT-PCR. The Ct values of genes, including CA6 (p = 0.0017), PON2 (p = 0.0210), TCL1A (p = 0.0064), and VPREB1 (p = 0.0338), were significant in Ph-like ALL. GSEA analysis identified VPREB1 as enrichment in the KRAS signaling pathway, and several genes that interact with VPREB1 were reported as critical molecules involved in the leukemogenesis of B-cell ALL.Conclusion: In summary, we demonstrate using a gene expression microarray for classifying Ph-like B-cell ALL and highlight VPREB1 as a potential biomarker for this disease.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality Assessment of Cryopreserved Peripheral Blood Stem Cell Products: Evaluation of Two Methods for Flow Cytometric Viability Testing. 冷冻保存的外周血干细胞产品的质量评估：两种流式细胞活力测试方法的评估。

International journal of laboratory hematology Pub Date : 2024-09-22 DOI: 10.1111/ijlh.14374

Vladimira Rimac, Ines Bojanić, Marijana Škifić, Sanja Dabelić, Branka Golubić Ćepulić

{"title":"Quality Assessment of Cryopreserved Peripheral Blood Stem Cell Products: Evaluation of Two Methods for Flow Cytometric Viability Testing.","authors":"Vladimira Rimac, Ines Bojanić, Marijana Škifić, Sanja Dabelić, Branka Golubić Ćepulić","doi":"10.1111/ijlh.14374","DOIUrl":"https://doi.org/10.1111/ijlh.14374","url":null,"abstract":"Introduction: The standard flow cytometry method for viability testing using 7-aminoactinomycin D (7-AAD) determines cells in necrosis and late apoptosis. The colony-forming unit (CFU) assay, which evaluates the proliferation ability of HSCs, is also used in graft quality assessment despite known deficiencies that make this assay impractical in routine clinical settings. The aim was to compare the effectiveness of the flow cytometry 7-AAD/annexin V method with the 7-AAD method in assessing the quality of HSCs in autologous and allogeneic peripheral blood stem cell (PBSC) products.Methods: Thirty autologous and 30 allogeneic fresh and thawed cryopreserved PBSC products were included in this study. The viability of HSCs was determined using the 7-AAD method and 7-AAD/annexin V method on a flow cytometer, while their clonogenic capacity was assessed by CFU assay.Results: There was an excellent correlation for CD34+ cell viability between the 7-AAD and the 7-AAD/annexin V method for fresh samples (Rs = 0.930, p < 0.001) and a good correlation for thawed PBSC samples (Rs = 0.739, p < 0.001). Excellent correlation was observed for post-thaw CD34+ cell recovery between the two methods for viability (Rs = 0.980, p < 0.001). Statistical analysis showed a weak correlation between CFU-GM recovery and CD34+ cell recovery, regardless of which viability testing method was used (7-AAD method p = 0.021, Rs = 0.298; 7-AAD/annexin V method p = 0.029, Rs = 0.282).Conclusions: Results of this study showed that in the quality assessment of cryopreserved PBSC product viability, the 7-AAD/annexin V method had no added value compared to the 7-AAD method, which was suitable enough for routine quality control of cryopreserved autologous and allogeneic PBSC samples.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does the VWF:CB Assay Help to Diagnose von Willebrand Factor Deficiency in Patients With a Bleeding Disorder of Unknown Cause? VWF:CB 检测法是否有助于诊断不明原因出血性疾病患者的冯-威廉因子缺乏症？

International journal of laboratory hematology Pub Date : 2024-09-20 DOI: 10.1111/ijlh.14371

Marc Trossaërt, Fabienne Genre-Volot, Valérie Horvais, Catherine Ternisien, Pierre Boisseau, Marc Fouassier, Nicolas Drillaud, Benjamin Gillet, Morgane Péré, Antoine Babuty, Emmanuelle Jeanpierre, Emmanuel de Maistre

{"title":"Does the VWF:CB Assay Help to Diagnose von Willebrand Factor Deficiency in Patients With a Bleeding Disorder of Unknown Cause?","authors":"Marc Trossaërt, Fabienne Genre-Volot, Valérie Horvais, Catherine Ternisien, Pierre Boisseau, Marc Fouassier, Nicolas Drillaud, Benjamin Gillet, Morgane Péré, Antoine Babuty, Emmanuelle Jeanpierre, Emmanuel de Maistre","doi":"10.1111/ijlh.14371","DOIUrl":"https://doi.org/10.1111/ijlh.14371","url":null,"abstract":"Introduction: The entity entitled bleeding disorder of unknown cause (BDUC) qualifies individuals displaying a mild haemorrhagic profile but normal routine coagulation tests. This study was designed to evaluate whether collagen-binding assay for von Willebrand Factor (VWF) measurement (VWF:CB) could allow to diagnose VW disease in such patients.Methods: A large screening was conducted prospectively in two University Hospitals, using the bleeding assessment tool (BAT) recommended by the International Society of Thrombosis and Hemostasis. Patients with an abnormal BAT were confirmed to have a normal complete hemostatic evaluation. A large range of VWF assays was then carried out on a new blood sample for the 68 individuals (91% women) thus identified. Of note, five VWF:CB using different types of collagen were performed, as well as a comprehensive sequencing of the VWF gene.Results: Of this cohort, only 3 individuals (all blood group O), had a VWF:CB between 40 and 50 IU/dL. No unknown anomaly of the VWF gene was disclosed. Of note, 54% of these patients had unexplained abnormal occlusion times on PFA-200.Conclusion: This study identified 68 cases of BDUC, after screening of a large population, indicating a low incidence. Only 3 cases were potentially confirmed as displaying moderate von Willebrand disease. VWF:CB tests were globally normal in the 65 other patients of the cohort.Trial registration: ClinicalTrials.gov identifier: NCT0279220.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Binucleated Lymphocytes With Globular Inclusions in Relapsed Splenic Marginal Zone Lymphoma. 复发性脾边缘区淋巴瘤中带有球状包涵体的双核淋巴细胞

International journal of laboratory hematology Pub Date : 2024-09-06 DOI: 10.1111/ijlh.14364

Verónica Roldán Galiacho, Marta Dueñas Usategui, Marta Alonso Varela, Elena Amutio, Juan Carlos García-Ruiz

引用次数: 0

Evaluation of the immature platelet fraction as a predictive marker of bone marrow regeneration after hematopoietic stem cell transplantation. 评估作为造血干细胞移植后骨髓再生预测标志的未成熟血小板部分。

International journal of laboratory hematology Pub Date : 2024-09-04 DOI: 10.1111/ijlh.14358

Kélian Steibel, Magalie Joris, Valentin Clichet, Amandine Charbonnier, Judith Desoutter, Jean-Pierre Marolleau, Loïc Garçon, Thomas Boyer

{"title":"Evaluation of the immature platelet fraction as a predictive marker of bone marrow regeneration after hematopoietic stem cell transplantation.","authors":"Kélian Steibel, Magalie Joris, Valentin Clichet, Amandine Charbonnier, Judith Desoutter, Jean-Pierre Marolleau, Loïc Garçon, Thomas Boyer","doi":"10.1111/ijlh.14358","DOIUrl":"https://doi.org/10.1111/ijlh.14358","url":null,"abstract":"Introduction: Hematopoietic stem cell transplantation (HCST) is a widely used therapy in the management of hematological malignancies, leading to cytopenias that require transient transfusions. Platelet recovery (PR) following HSCT is assessed by monitoring platelet count (PC). Immature platelet fraction (IPF) is a research parameter offered by Sysmex® on XN series analyzers, enabling rapid diagnostic orientation in the event of thrombocytopenia. It has also been described as a predictive factor for PR after chemotherapy or HSCT, and thresholds have been proposed.Methods: The objective of this study was to assess the predictive capability of IPF for PR in a prospective cohort of patients undergoing HSCT and to evaluate its utility in guiding platelet transfusion decision.Results: An optimized A-IPF (absolute number of IPF) threshold of 2.5 × 109/L was predictive of a PC greater than 50 × 109/L at day 30 with a sensitivity of 78.9%, specificity of 78.6%, positive predictive value (PPV) of 83.3% and negative predictive value (NPV) of 73.3%. We were able to distinguish patients recovering PC before day 15 with an earlier %IPF peak, greater IPF recovery kinetics and faster neutrophil recovery.Conclusion: A-IPF shows promise as a predictor of PR following HSCT. A multicenter study could help confirm both A-IPF and %IPF (IPF) clinical utility before it is made available to clinicians.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence-Based Platelet Count, Blood Smear and Pre-Analytics Are Decisive in a Case of Fibrin Strand Interference Masking Severe Thrombocytopenia in an ITP Patient. 基于荧光的血小板计数、血涂片和预分析在一例因纤维蛋白束干扰而掩盖严重血小板减少的 ITP 患者中起决定性作用。

International journal of laboratory hematology Pub Date : 2024-09-03 DOI: 10.1111/ijlh.14367

Marnix Mylemans, Nancy Boeckx, Ann Janssens, Mercedeh Tajdar, Christine Van Laer

引用次数: 0

Delta Immature Platelet Fraction Is Associated With Mortality in Bacteremia Patients. 德尔塔不成熟血小板比例与菌血症患者的死亡率有关。

International journal of laboratory hematology Pub Date : 2024-09-02 DOI: 10.1111/ijlh.14365

Pei-Chun Shih, Yi-Hua Wang, Shey-Ying Chen, Min Tseng, Cheng-An Hsu, Ming-Yan Yang, Hsin-Yao Wang, Jia-Arng Lee

{"title":"Delta Immature Platelet Fraction Is Associated With Mortality in Bacteremia Patients.","authors":"Pei-Chun Shih, Yi-Hua Wang, Shey-Ying Chen, Min Tseng, Cheng-An Hsu, Ming-Yan Yang, Hsin-Yao Wang, Jia-Arng Lee","doi":"10.1111/ijlh.14365","DOIUrl":"https://doi.org/10.1111/ijlh.14365","url":null,"abstract":"Objectives: Immature platelet fraction (IPF) for differentiating bacteremia has been explored, whereas its prognostic correlation remains uncertain. This study aims to confirm the predictive capability of IPF for bacteremia and investigate its association with prognosis.Methods: Patients with complete blood count (CBC) on the blood culture day (D1) and the preceding day (D0) were retrospectively recruited and categorized into bacteremia and nonbacteremia groups. Immature platelet (IP) analysis, alongside CBC, was conducted. Delta IPF, defined by the absolute values of D1 minus D0 results was calculated. The ability to distinguish bacteremia from nonbacteremia patients, and the correlation with mortality were analyzed.Results: From February to December 2020, a total of 150 patients were enrolled, with 75 having bacteremia. The specificity for delta IPF ≥3.4% to predict bacteremia was 97.3% (95% confidence interval [CI]: 90.7-99.7). When delta IPF ≥3.4% combined with procalcitonin ≥0.5 (ng/mL), the sensitivity was 90.5% (95% CI: 69.6%-98.8%). Within the bacteremia group, delta IPF and the proportion of patients with delta IPF ≥1.5% were significantly higher in nonsurvival, while delta platelet levels did not. Furthermore, delta IPF ≥1.5% was independently associated with 30-day mortality (adjusted odds ratio: 3.88, 95% CI: 1.2%-11.4%; p = 0.020). The 30-day survival curve demonstrated a significant difference between patients with delta IPF ≥1.5% and those without (p < 0.001).Conclusions: Delta IPF correlates with mortality in bacteremia patients. Our findings suggest IPF not only helps detect bacteremia but also predicts prognosis in the early stage.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142121436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing reference intervals for extended red blood cell parameters with the Mindray BC-6800Plus hematology analyzer in a Chinese population. 在中国人群中使用明德 BC-6800Plus 血液分析仪建立扩展红细胞参数参考区间。

International journal of laboratory hematology Pub Date : 2024-08-23 DOI: 10.1111/ijlh.14361

Yanping Luo, Shuaiyan Wang, Zhuocheng Chen, Shan Lin, Sanping Guo, Hongmei Mo

引用次数: 0

Brief communication: Heparin-calibrated chromogenic anti-Xa assay for the detection of threshold-levels of direct oral anticoagulants. 简要通讯：用于检测直接口服抗凝剂阈值水平的肝素校准显色抗 Xa 试验。

International journal of laboratory hematology Pub Date : 2024-08-23 DOI: 10.1111/ijlh.14357

Delphine De Smet, Dimitri Hemelsoet, Veerle De Herdt, Pieter M De Kesel, Katrien M J Devreese

引用次数: 0