International journal of laboratory hematology最新文献

{"title":"Blood Cell Counts and the Duffy Null Phenotype: Beyond Neutropenia.","authors":"Elvira Deolinda R P Velloso, Andreza Oliveira Dos Santos, Carla Luana Dinardo, Carolina Bonet Bub, Leandro Dinalli Santos, Mauren Fernanda Moller Dos Santos, João Carlos de Campos Guerra, Denise Dos Anjos Laurentis de Sousa Campos, Monica Juliana Vieira, Fábio Julio da Luz, Alfredo Mendrone-Junior, Rosana M Cosentino Penteado","doi":"10.1111/ijlh.70235","DOIUrl":"https://doi.org/10.1111/ijlh.70235","url":null,"abstract":"<p><p>The absence of Duffy antigen expression on red blood cells, caused by a single nucleotide polymorphism in the DARC/ACKR1 gene, confers protection against malaria and is associated with lower absolute neutrophil counts (ANC) in some studied populations. This study aimed to establish reference intervals for ANC and evaluate other hematologic parameters influenced by Duffy status in Brazilian individuals. In this prospective study, complete blood counts and Duffy phenotyping were performed in 311 healthy adult blood donors from two independent blood banks. Among participants, 51.4% were male, with a median age of 37 years, and 46.6% self-identified as Black or mixed-race. The Duffy null phenotype was observed in 146 individuals (46.9%), with lower male representation and higher prevalence among Black individuals. Duffy null individuals showed lower median white blood cell counts (5350 vs. 7060/μL) and ANC (median 2250 vs. 4070/μL), with a 95% reference range of 1180-4800/μL versus 2200-7250/μL. They also had a lower neutrophil percentage and higher relative counts of other leukocytes, without differences in absolute lymphocytes and eosinophils. Additionally, they exhibited higher erythrocyte counts, hemoglobin, hematocrit, platelet counts, and mean platelet volume. These findings support Duffy-specific reference intervals to avoid misclassification in diverse populations like Brazil.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of IgM Monoclonal Paraprotein in Bone Marrow Aspiration: The Role of Immunophenotyping by Flow Cytometry in Selecting Cases for Molecular Testing for the p.MYD88 L265P Point Mutation.","authors":"Georgios Boutsikas, Konstantinos Agiannitopoulos, Elisavet Kouvidi, Myrto Katraki, Athanasios Papatheodorou, Anna Roussou, Nikolaos Paschalidis, Theodoros P Vassilakopoulos, Ioannis Anagnostopoulos, Dimitra Sasaroli, Aglaia Dimitrakopoulou","doi":"10.1111/ijlh.70237","DOIUrl":"10.1111/ijlh.70237","url":null,"abstract":"<p><strong>Background: </strong>Flow cytometry and real-time quantitative polymerase chain reaction (qRT-PCR) for MYD88 mutations (MYD88mt) are routinely performed on bone marrow aspirates during the diagnostic evaluation of IgM monoclonal paraproteinemia. The potential predictive value of immunophenotypic findings for molecular results, however, remains to be fully clarified.</p><p><strong>Objective: </strong>This study aimed to determine whether the presence or absence of clonal B-cells detected by flow cytometry in bone marrow aspirates can predict MYD88mt status as assessed by qRT-PCR.</p><p><strong>Methods: </strong>A total of 88 cases with IgM monoclonal paraprotein and available bone marrow immunophenotyping and MYD88 mut qRT-PCR results were retrospectively analyzed. Flow cytometry was used to identify clonal B-cell populations, and results were correlated with molecular findings.</p><p><strong>Results: </strong>The absence of clonal B-cells by flow cytometry demonstrated a high negative predictive value (NPV) of 96% for MYD88mt. Conversely, the presence of a clonal B-cell population showed a positive predictive value (PPV) of 62% for detecting MYD88mt by molecular testing.</p><p><strong>Conclusions: </strong>Bone marrow immunophenotyping by flow cytometry may serve as an effective screening tool to stratify patients with IgM monoclonal paraproteinemia for MYD88mt molecular testing. Specifically, the absence of clonal B-cells strongly predicts a negative molecular result, potentially reducing unnecessary molecular analyses.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blasts Showing Apparent Engulfment of Apoptotic Debris in Apoptosis-Rich Acute Myeloid Leukemia With NPM1, FLT3-ITD, DNMT3A, and TET2 Mutations and Trisomy 8.","authors":"Zhaodong Xu","doi":"10.1111/ijlh.70234","DOIUrl":"https://doi.org/10.1111/ijlh.70234","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148851800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Loss of Surface CD3 and CD45 in a TCL1-Negative T-Prolymphocytic Leukemia With Concurrent CLL/SLL.","authors":"Sameer Andani, Dietrich Werner Idiaquez, Julia Wang, Samuel B Reynolds, Mingfei Yan","doi":"10.1111/ijlh.70232","DOIUrl":"https://doi.org/10.1111/ijlh.70232","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148852075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Performance of Reticulocyte Hemoglobin Content (CHr) for Iron Deficiency in Chronic Kidney Disease: A Reanalysis of a Published South African Dataset.","authors":"May AlMoshary, Ahmad Ali Alharbi, Ekremah A Alzarea, Alia Alfaraedi, Omar A Alswailmi, Lamia Abdelbary Gad, Ezeldine K Abdalhabib, Mohammad Saeed Aldosari, Mohamed Hanafy Morsy, Musaad B Alsahly, Omran Shrebaty","doi":"10.1111/ijlh.70229","DOIUrl":"https://doi.org/10.1111/ijlh.70229","url":null,"abstract":"<p><strong>Introduction: </strong>Iron deficiency (ID) diagnosis in chronic kidney disease (CKD) is complicated by the limited reliability of ferritin as an acute-phase reactant. Reticulocyte hemoglobin content (CHr) provides a direct measure of iron available for erythropoiesis. We aimed to validate the diagnostic performance of CHr using a real-world dataset.</p><p><strong>Methods: </strong>We performed a cross-sectional diagnostic accuracy analysis of the Nalado et al. dataset (n = 399; 258 CKD, 141 controls). CHr was measured on a Siemens ADVIA 2120 analyzer. ID was defined as absolute (AID; ferritin < 100 ng/mL and TSAT < 20%) or functional (FID; ferritin ≥ 100 ng/mL and TSAT < 20%). ROC analysis evaluated diagnostic performance. Multivariate logistic regression, adjusted for age, sex, and CKD status, assessed the independent predictive value of CHr.</p><p><strong>Results: </strong>ID was present in 104 participants (26.1%). CHr was significantly lower in the ID group (28.0 ± 3.3 vs. 29.9 ± 2.7 pg; p < 0.0001). CHr yielded an AUC of 0.672 (95% CI: 0.610-0.731) with an optimal cut-off of 29.2 pg (sensitivity 64.4%, specificity 64.4%). In the CKD subgroup, performance improved (AUC = 0.738). CHr showed no correlation with ferritin (ρ = 0.010, p = 0.844). In multivariate analysis, CHr independently predicted ID (adjusted OR = 0.786; 95% CI: 0.709-0.872; p < 0.0001).</p><p><strong>Conclusion: </strong>CHr is a moderately useful, independent laboratory marker for iron-restricted erythropoiesis in CKD. Its lack of dependence on ferritin suggests value when standard biomarker interpretation is ambiguous. Prospective studies with concurrent inflammatory markers are warranted.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148820696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myelodysplasia-Related Gene Mutation Burden Is Associated With Complete Remission After Induction Therapy in Acute Myeloid Leukemia, Not Otherwise Specified: Reclassification According to the Updated Classifications.","authors":"Taegeun Lee, Daehyun Chu, Miyoung Kim, Young-Uk Cho, Sang-Hyun Hwang, Je-Hwan Lee, Yunsuk Choi, Eun-Ji Choi, Ho Joon Im, Hyery Kim, Seongsoo Jang","doi":"10.1111/ijlh.70230","DOIUrl":"https://doi.org/10.1111/ijlh.70230","url":null,"abstract":"<p><strong>Introduction: </strong>Updated classifications incorporating myelodysplasia-related (MR) gene mutations reclassify many cases previously diagnosed as acute myeloid leukemia, not otherwise specified (AML, NOS). We evaluated the clinical significance of MR gene mutation burden, defined by mutation number and variant allele frequency (VAF), in AML, NOS.</p><p><strong>Methods: </strong>We retrospectively reviewed adults diagnosed with AML, NOS who underwent next-generation sequencing between 2018 and 2023. After excluding cases with KMT2A rearrangements or biallelic TP53 inactivation, 95 patients were included. Associations between MR gene mutation burden and complete remission (CR) failure were assessed using logistic regression; exploratory survival analyses were performed.</p><p><strong>Results: </strong>MR gene mutations were identified in 57.9%-63.2% of patients. For CR analysis, 89 patients were evaluable after excluding five treated with FLT3 inhibitors and one lost to follow-up. The CR rate was lower in patients with MR gene mutations than in those without MR gene mutations (39.7% vs. 64.5%, p = 0.025). Patients with ≥ 2 MR gene mutations had the lowest CR rate (29.7%). An exploratory ROC-derived VAF cutoff of 28.6% predicted CR failure with modest discriminatory performance (AUC, 0.658). In multivariate analysis, the highest VAF among MR gene mutations was independently associated with CR failure (odds ratio, 1.022; p = 0.028). Exploratory survival analyses showed inferior outcomes in patients with high-VAF MR gene mutations.</p><p><strong>Conclusion: </strong>A greater number of MR gene mutations and higher VAF were associated with inferior early treatment response in AML, NOS. MR gene mutation burden may provide additional prognostic information among patients reclassified as AML with MR gene mutations.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148820787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Non-Classic PML/RARA FISH Pattern Revealing a Novel Five-Way Translocation in Acute Promyelocytic Leukemia: Implications for Diagnosis and Molecular Follow-Up.","authors":"Bosung Park, Jieun Kim, Hae In Bang, Seug Yun Yoon, Byung Ryul Jeon, Rojin Park","doi":"10.1111/ijlh.70226","DOIUrl":"https://doi.org/10.1111/ijlh.70226","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148835822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re: Comparison of Blood Cell Count Results Between MgSO₄ and K₂EDTA. Stability and Accuracy of Platelet Counting With MgSO₄.","authors":"Supriya S Desai, Rajesh Prasad Jayaswal, Maajid Mohi Ud Din Malik","doi":"10.1111/ijlh.70231","DOIUrl":"https://doi.org/10.1111/ijlh.70231","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpha Spectrin Null Alleles and Low Expression Polymorphisms-A Hemolytic Phenotype With Normal Band 3 Testing.","authors":"Ryan C Shean, Archana M Agarwal","doi":"10.1111/ijlh.70227","DOIUrl":"https://doi.org/10.1111/ijlh.70227","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point-of-Care White Blood Cell Differential Testing in Pediatric Inpatients (2 Months-17 Years): How Does HemoScreen Perform?","authors":"Alessio Correani, Beatrice Niccoletti, Marianna Pavani, Michela Sampaolo, Lucia Finaurini, Michela Pelloso, Martina Montagnana, Marco Moretti","doi":"10.1111/ijlh.70228","DOIUrl":"https://doi.org/10.1111/ijlh.70228","url":null,"abstract":"<p><strong>Background: </strong>Point-of-care (POC) hematology testing is of growing interest in pediatric practice. We previously evaluated an image-based POC hematology analyzer (HemoScreen, PixCell Medical, Yokneam Illit, Israel) for total white blood cell (WBC), red blood cell, and platelet counts and associated indices in pediatric inpatients. Given the diagnostic importance of WBC differentials, this study aimed to extend the evaluation to five-part differential counts.</p><p><strong>Methods: </strong>We prospectively analyzed 141 venous K₂-EDTA samples from pediatric inpatients aged 2 months-17 years. Complete blood counts were obtained with the Sysmex XN-1000 (Sysmex Co., Kobe, Japan) and reanalyzed with HemoScreen. Blood smears were digitized with CellaVision DC-1 (CellaVision AB, Lund, Sweden) and reviewed by cytologists. HemoScreen WBC differential percentages were directly compared with cytologist-reviewed digital microscopy, whereas absolute counts were compared with composite values calculated from microscopy-derived percentages and the Sysmex WBC count. Analytical agreement, diagnostic concordance and flagging performance were assessed.</p><p><strong>Results: </strong>Of 141 samples, 138 were suitable for analysis. Correlations for WBC subsets were strong to excellent (ρ = 0.625-0.977). Mean bias was within or close to EFLM-derived acceptable limits for neutrophils and lymphocytes. Diagnostic agreement was excellent for neutropenia, neutrophilia, and lymphocytopenia, and good for lymphocytosis, monocytosis, and eosinophilia. HemoScreen flagged 31.9% of samples for abnormal cells, with sensitivity 74.1%, specificity 78.4%, positive predictive value 45.5%, negative predictive value 92.6%, and overall efficiency 77.5%.</p><p><strong>Conclusions: </strong>In pediatric venous samples, HemoScreen showed good analytical and diagnostic performance for the five-part WBC differentials. Its abnormal-cell flagging may support prioritization of morphological review, although its workflow impact requires further evaluation.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}