International journal of laboratory hematology最新文献

{"title":"Fibrinogen Dusart Escapes Clauss-CWA Screening in a Genotype-Confirmed Dysfibrinogenemia Cohort.","authors":"Laetitia Folly-Muller, Emmanuel De Maistre, Tomas Simurda, Rita Marchi, Pierre Fontana, Alessandro Casini","doi":"10.1111/ijlh.70244","DOIUrl":"https://doi.org/10.1111/ijlh.70244","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue Factor-Positive Platelets: An Additional Dimension of Platelet Flow Cytometric Phenotyping.","authors":"Marina Camera","doi":"10.1111/ijlh.70245","DOIUrl":"https://doi.org/10.1111/ijlh.70245","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defining Reference Intervals of Emerging Extended Inflammatory Parameters (EIP) in a Population-Based Study.","authors":"Karan Paisooksantivatana, Pawadee Chinudomwong, Atisak Jiaranaikulwanich, Mintra Tanakitivirul","doi":"10.1111/ijlh.70240","DOIUrl":"https://doi.org/10.1111/ijlh.70240","url":null,"abstract":"<p><strong>Introduction: </strong>Extended inflammatory parameters (EIPs), including neutrophil reactivity intensity (NEUT-RI), neutrophil granularity intensity (NEUT-GI), reactive lymphocytes (RE-LYMP), and antibody-synthesizing lymphocytes (AS-LYMP), provide a rapid assessment of leukocyte activation. These parameters show diagnostic and prognostic value in infections and inflammatory diseases. However, their clinical application remains limited by the lack of standardized reference intervals (RIs), particularly in Southeast Asian populations. This study established population-based RIs for EIPs in healthy Thai adults.</p><p><strong>Methods: </strong>Reference intervals were established following CLSI EP28-A3c guidelines. Healthy adults were included if they had no history of chronic disease, recent infection or inflammation, pregnancy, or medication use affecting white blood cells, and demonstrated normal CBC results without qualitative flags. Outliers were excluded using the Tukey method. Non-parametric methods were applied to calculate RIs, and the Harris and Boyd method was used to evaluate the need for age- and sex-specific partitioning.</p><p><strong>Results: </strong>Most EIPs did not require demographic partitioning. Reference intervals of NEUT-GI ranged from 146.7 to 160.0 scatter intensity (SI) units, and NEUT-RI ranged from 42.4 to 52.2 fluorescence intensity (FI) units. AS-LYMP% ranged from 0.0% to 0.4%, while AS-LYMP# ranged from 0.00 to 0.02 × 10⁹/L. However, RE-LYMP% and RE-LYMP# demonstrated significant sex-related differences requiring sex-specific RIs. Female RE-LYMP% ranged from 0.20% to 1.10% and male values from 0.10% to 0.90%, while RE-LYMP# ranged from 0.01 to 0.08 × 10⁹/L in females and 0.01 to 0.06 × 10⁹/L in males.</p><p><strong>Conclusion: </strong>Reference intervals for NEUT-GI, NEUT-RI, RE-LYMP, and AS-LYMP were established in healthy Thai adults. Most EIPs did not require demographic partitioning, whereas RE-LYMP% and RE-LYMP# required sex-specific intervals.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudo-Reticulocytosis in Severe Plasmodium falciparum Malaria: A Sysmex XN-9100 Scattergram Pitfall.","authors":"Bushra Al Abri, Maryam Al Bakri","doi":"10.1111/ijlh.70249","DOIUrl":"https://doi.org/10.1111/ijlh.70249","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICSH Guidance on Bone Marrow Examination and Reporting.","authors":"Wendy N Erber, Alexandar Tzankov, Olga Pozdnyakova, Hiromichi Matsushita, Gina Zini, Leonie Saft","doi":"10.1111/ijlh.70214","DOIUrl":"https://doi.org/10.1111/ijlh.70214","url":null,"abstract":"<p><p>Examination of the bone marrow (BM) remains fundamental to the diagnosis, classification, prognostication, and monitoring of hematolymphoid and other disorders affecting blood cell production. Since publication of the International Council for Standardization in Haematology (ICSH) guideline in 2008, advances in diagnostic technologies, disease classification systems and laboratory reporting practices have significantly evolved. These developments necessitate a framework to support contemporary BM evaluation and reporting. This document, prepared by an international ICSH Working Party, provides updated guidance on the examination and reporting of BM specimens and is an extension and refinement of the 2008 ICSH guidelines. It outlines the appropriate application and integration of key diagnostic modalities, including morphological assessment of BM aspirate and trephine biopsy specimens, immunophenotypic analysis by flow cytometry and immunohistochemistry and molecular genetic testing. Central to this guidance is the principle of integrated reporting, whereby findings from all diagnostic modalities are synthesized within the clinical context and aligned with current international classification systems. The ICSH acknowledges the emergence of automated digital microscopy, computational decision support tools, and other technological innovations in BM analysis. These developments hold considerable promise, but whilst many remain under evaluation, specific guidance regarding their routine clinical implementation is beyond the scope of this document.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Laboratory Associations of JAK2 V617F Variant Allele Frequency in Patients With JAK2 V617F-Positive Erythrocytosis: A Single-Centre Retrospective Analysis.","authors":"Tahir Alper Cinli, Çisem Çınar","doi":"10.1111/ijlh.70242","DOIUrl":"https://doi.org/10.1111/ijlh.70242","url":null,"abstract":"<p><strong>Background: </strong>JAK2 V617F variant allele frequency (VAF) reflects clonal burden in myeloproliferative neoplasms, but the clinical associations of low-level VAF in patients with erythrocytosis remain uncertain.</p><p><strong>Objective: </strong>The objective of this study is to examine associations between diagnostic JAK2 V617F VAF categories and clinical, laboratory, bone marrow and thrombotic outcomes in patients with JAK2 V617F-positive erythrocytosis.</p><p><strong>Patients and methods: </strong>This single-centre retrospective cohort included 174 adults. Patients were stratified by diagnostic VAF as low (< 5%; n = 62), intermediate (5%-50%; n = 81) or high (> 50%; n = 31). Clinical and laboratory characteristics, bone marrow findings and incident thrombotic events during follow-up were compared. Multivariable logistic regression was used to evaluate factors associated with thrombosis.</p><p><strong>Results: </strong>Follow-up duration was comparable amongst the VAF groups (median, 5.0 vs. 8.0 vs. 6.0 years; p = 0.227). Thrombosis rates increased across the groups (12.9% vs. 22.2% vs. 48.4%; p = 0.001), as did splenomegaly (25.8% vs. 53.1% vs. 64.5%; p < 0.001) and myelofibrosis transformation (1.6% vs. 13.6% vs. 20.0%; p = 0.011). Amongst patients with available measurements, median serum erythropoietin decreased with higher VAF (8.0 vs. 3.1 vs. 2.2 mIU/mL; p < 0.001), whereas the proportion with subnormal erythropoietin increased (17.8% vs. 47.6% vs. 82.6%; p < 0.001). Amongst biopsied patients, polycythemia vera-supportive bone marrow morphology was present in 50.0%, 93.5% and 100% of the groups respectively. A diagnostic VAF > 50% was independently associated with subsequent thrombosis (OR: 3.35; 95% CI: 1.06-10.58; p = 0.039), as was platelet count (OR: 9.92; 95% CI: 1.52-64.63; p = 0.016).</p><p><strong>Conclusions: </strong>A diagnostic JAK2 V617F VAF > 50% was independently associated with subsequent thrombosis in this cohort. The low-VAF group was diagnostically and clinically heterogeneous. These findings should be considered hypothesis-generating and require prospective validation.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance Assessment of Staclot-LA Assay on Fully Automated Coagulation Analyser for Lupus Anticoagulant Testing Using Internal and External Quality Controls.","authors":"V Siguret, G Jourdi, S Melicine, L Goubeau, Q Pomorski, B Joly-Laffargue, A Stepanian, M Delrue, C Bon, P Meijer, E Curis","doi":"10.1111/ijlh.70241","DOIUrl":"https://doi.org/10.1111/ijlh.70241","url":null,"abstract":"<p><strong>Introduction: </strong>It is recommended to detect lupus anticoagulant (LA) using two phospholipid-dependent clotting assays, including a LA-sensitive activated partial-thromboplastin-time (aPTT). Among these reagents, Staclot-LA assay is performed on a mixture of test and normal plasma with and without hexagonal phase phospholipids. However, its lack of validation on automated coagulation analyser is a major drawback. We aimed i/to assess the analytical performance of Staclot-LA assay on automated system; ii/to compare inter-laboratory agreement of Staclot-LA performed on semi-automated or fully automated analysers using two external-quality-assurance (EQA) programs data.</p><p><strong>Methods: </strong>We adapted the Staclot-LA assay (Stago) set-up on a STA-R-Max analyser and locally validated the manufacturer's recommended cut-off for positive LA testing using samples from healthy volunteers. Reproducibility and accuracy were assessed using internal quality controls (IQC, Staclot-LA1/LA2) over a five-year period. Staclot-LA results from EQA Probioqual and ECAT were compared between semi- and fully-automated analysers.</p><p><strong>Results: </strong>Agreement of our LA results (positive or negative) was observed in 100% of IQC (n = 130) and EQA (n = 58) samples. Probioqual and ECAT interlaboratory Staclot-LA results showed full agreement for 96.2% and 89.7% in semi-automated system users vs 96.7% and 92.5% in automated system users. The system had no significant impact on the qualitative results (Probioqual p = 1.000; ECAT p = 0.5912), even though we observed increasing biases of the differences in clotting times, especially in LA positive samples on automated vs semi-automated analysers.</p><p><strong>Conclusion: </strong>Staclot-LA assay can be reliably performed on a fully automated system, thus paving the way for wider accessibility and widespread use.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148882136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Giant Elliptical Platelets as a Cytological Component in MYH9-Related Disease.","authors":"Márcio Antonio W Melo, Cristina M Silveira, Maíra M Ribeiro, Evilly R O Magalhães, Gabriela S Arcanjo","doi":"10.1111/ijlh.70238","DOIUrl":"https://doi.org/10.1111/ijlh.70238","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148875944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generative Artificial Intelligence in Peripheral Blood Morphology: Synthetic Image Creation and Digital Stain Normalization.","authors":"Anna Merino, Kevin Barrera, Santiago Alférez, José Rodellar","doi":"10.1111/ijlh.70239","DOIUrl":"https://doi.org/10.1111/ijlh.70239","url":null,"abstract":"<p><p>The morphological analysis of peripheral blood cells is increasingly adopting deep learning, mainly for the automatic recognition of the variety of normal and abnormal cell classes. However, its clinical application is limited by the scarcity of annotated datasets for rare diseases and the high variability of staining protocols between laboratories. This paper provides an overview of how Generative Artificial Intelligence methods can help overcome these obstacles. Firsts, some basic concepts are presented, distinguishing between discriminative AI and generative AI, specifically generative adversarial networks (GANs) and diffusion models. The core of the paper addresses two problems: (1) the automatic generation of artificial blood cell images and (2) the digital artificial staining to reduce inter-laboratory differences. In these two cases, three sections are included: underlying concepts, a literature review and practical examples. These show how high-quality images of blood cells with realistic morphological characteristics are created, which strengthen the classifier's training. In multicenter tests, the examples illustrate that normalizing the staining allows a classifier trained on abnormal blood cell images from a single hospital to accurately recognize abnormal cells obtained in other laboratories, significantly improving performance without distorting cell morphology. A final section concludes the paper with observations and future perspectives on how generative tools can assist clinical pathologists as decision support systems that can operate consistently across diverse clinical settings.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of the Sysmex DI-60 Into the Laboratory's Manual Slide Review Criteria: Assessment of Laboratory Efficiency in a Routine Practice.","authors":"Eunju Shin, Mina Hur, Hanah Kim, Mi Hyun Hong, Hee-Won Moon, Yeo-Min Yun","doi":"10.1111/ijlh.70236","DOIUrl":"https://doi.org/10.1111/ijlh.70236","url":null,"abstract":"<p><strong>Introduction: </strong>Digital morphology (DM) analyzers may improve laboratory efficiency; however, their practical integration into manual slide review (MSR) criteria and the resulting impact on overall efficiency remain underexplored. We evaluated the efficiency of the DI-60 (Sysmex, Kobe, Japan) by assessing its potential to modify or replace existing MSR criteria in a routine practice and comparing its turnaround time (TAT) with that of manual counting.</p><p><strong>Methods: </strong>A total of 907 samples were consecutively selected based on our laboratory's MSR criteria. We simulated modified MSR criteria incorporating the DI-60 to assess the potential reduction in laboratory workload. We compared TATs of the DI-60 and manual counting according to white blood cell (WBC) counts (range, 0.01-158.6 × 10⁹/L).</p><p><strong>Results: </strong>DI-60 achieved complete analysis in 717/907 samples (79.1%), showing stable performance in samples with normal WBC counts. However, the overall TAT of the DI-60 was significantly longer than that of manual counting (263 vs. 120 s, p < 0.001). Incomplete analysis (< 200-cell count) occurred in 190 samples (20.9%), predominantly in hematology-oncology samples (94.7%) and samples with severe leukopenia. Simulation of modified MSR criteria incorporating the DI-60 demonstrated a potential workload reduction of 11.6% (105/907).</p><p><strong>Conclusions: </strong>DM analyzers may have the potential to reduce laboratory workload by being incorporated into each laboratory's MSR criteria. With incomplete analyses in specific sample types, however, DM analyzers cannot fully replace MSR. The effective use of DM analyzers requires streamlined integration into the existing laboratory workflow and further optimization considering each laboratory's situation and unmet needs.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}