International journal of laboratory hematology最新文献

Correction to "Improving Platelet Function Following Prophylactic Platelet Transfusion in Patients With Hematological Malignancies". 对“血液恶性肿瘤患者预防性输血小板后血小板功能改善”的修正。

International journal of laboratory hematology Pub Date : 2025-05-29 DOI: 10.1111/ijlh.14481

引用次数: 0

Pitfalls in Argatroban Monitoring: Heparin Interference With Dilute Thrombin Time Assays. 阿加曲班监测的缺陷：肝素干扰稀释凝血酶时间测定。

International journal of laboratory hematology Pub Date : 2025-05-26 DOI: 10.1111/ijlh.14505

Agathe Herb, Jordan Wimmer, Laurent Mauvieux, Laurent Sattler

引用次数: 0

Trisomy 8 in De Novo Acute Myeloid Leukemia Lacking MDS-Related Cytogenetics Does Not Significantly Influence Survival. 缺乏mds相关细胞遗传学的新生急性髓系白血病患者的8三体不显著影响生存

International journal of laboratory hematology Pub Date : 2025-05-26 DOI: 10.1111/ijlh.14504

Adelaide J Kwon, Miguel D Cantu, Yazan F Madanat, Robert P Hasserjian, Prasad Koduru, Sa Wang, Guilin Tang, Olga K Weinberg

{"title":"Trisomy 8 in De Novo Acute Myeloid Leukemia Lacking MDS-Related Cytogenetics Does Not Significantly Influence Survival.","authors":"Adelaide J Kwon, Miguel D Cantu, Yazan F Madanat, Robert P Hasserjian, Prasad Koduru, Sa Wang, Guilin Tang, Olga K Weinberg","doi":"10.1111/ijlh.14504","DOIUrl":"https://doi.org/10.1111/ijlh.14504","url":null,"abstract":"Introduction: The 2022 WHO and ICC classifications identify MDS-related cytogenetic abnormalities and secondary gene mutations (SM) that in de novo disease are diagnostic of myelodysplasia-related AML, which confers a poorer prognosis. While most MDS-related abnormalities overlap between the two classifications, trisomy 8 (+8) is unique to the ICC and has not been previously included as an MDS-related abnormality. In light of this, we sought to determine the prognostic significance of +8 as an MDS-related abnormality in patients with de novo AML lacking other MDS-related cytogenetics.Methods: We identified 337 patients with de novo AML lacking MDS-related cytogenetics other than +8 and analyzed clinicopathologic outcomes, overall survival (OS), and relapse-free survival (RFS). Two groups were identified: AML with SM (n = 195, 57.9%) and AML without SM (n = 142, 42.1%). Fifty-nine (17.5%) patients had +8; 39 (66.1%) of these had at least one SM, while 20 (33.9%) did not.Results: Among patients treated with induction or hypomethylating agents (n = 317), OS and RFS were significantly shorter in patients with SM than without (OS: p = 0.001, RFS: p = 0.0004) but not significantly different between patients with and without +8 (OS: p = 0.15, RFS: p = 0.35). Similarly, when cases were limited to those with SM (n = 179), no significant difference in OS or RFS was observed between patients with and without +8 (OS: p = 0.21, RFS: p = 0.30). There was no significant association between +8 and SM (p = 0.15).Conclusion: In our cohort, unlike MDS-related SM, trisomy 8 does not influence OS or RFS, despite its inclusion in the ICC as an MDS-related abnormality.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic ctDNA Monitoring: A Primary Tool Predictive of Response in a Patient on CAR-T Cell Therapy. 动态ctDNA监测：预测CAR-T细胞治疗患者反应的主要工具。

International journal of laboratory hematology Pub Date : 2025-05-25 DOI: 10.1111/ijlh.14503

Bhargava Rahul, Nathany Shrinidhi, Sen Ishita, M Kumar Nikhil, Yadav Chitresh, Swaminathan Anusha, Thakrani Darshan, Verma Kanika, Saini Manish, Dua Vikas

{"title":"Dynamic ctDNA Monitoring: A Primary Tool Predictive of Response in a Patient on CAR-T Cell Therapy.","authors":"Bhargava Rahul, Nathany Shrinidhi, Sen Ishita, M Kumar Nikhil, Yadav Chitresh, Swaminathan Anusha, Thakrani Darshan, Verma Kanika, Saini Manish, Dua Vikas","doi":"10.1111/ijlh.14503","DOIUrl":"https://doi.org/10.1111/ijlh.14503","url":null,"abstract":"Introduction: Dynamic monitoring of circulating tumor DNA (ctDNA) offers a non-invasive method to track treatment response in malignancies. While well-established in solid tumors, its role in lymphomas, especially in predicting response to CAR-T cell therapy, remains underexplored-more so in the Indian context. This case highlights ctDNA as a potential predictive biomarker in relapsed/refractory DLBCL undergoing CAR-T therapy.Case report: A 48-year-old male with transformed follicular lymphoma to DLBCL, refractory to R-CHOP and BR, was treated with anti-CD19 CAR-T cell therapy. Baseline ctDNA profiling from plasma revealed a TP53 p.E286K mutation at 1.3% VAF. Serial monitoring showed a decline to 0.4% at four weeks and complete clearance at eight weeks post-infusion, correlating with metabolic complete response on PET-CT. No co-occurring mutations were observed.Discussion: This case illustrates how dynamic ctDNA profiling can reflect early molecular response, preceding radiological confirmation. Existing literature suggests that early ctDNA negativity post-CAR-T correlates with improved outcomes. This is the first reported Indian case employing a validated, homebrew NGS-based ctDNA assay to longitudinally track CAR-T response. Incorporating ctDNA-guided surveillance may refine response assessment and reduce unnecessary imaging, optimizing outcomes in resource-constrained settings.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

International Committee for Standardization in Hematology (ICSH) Guidance on the Validation of Laboratory Developed Tests in Haemostasis. 国际血液学标准化委员会（ICSH）实验室开发的止血试验验证指南。

International journal of laboratory hematology Pub Date : 2025-05-22 DOI: 10.1111/ijlh.14502

Chris Gardiner, Akbar Dorgalaleh, Marión Echenagucia, Robert C Gosselin, Teruto Hashiguchi, Mingting Peng, Alan Neal, Steve Kitchen

引用次数: 0

Diagnostic Value of Botryoid Nuclei as a Biomarker of Severe Hyperthermia and Systemic Inflammation in Heatstroke and Neuroleptic Malignant Syndrome: A Challenge of Climate Change. 在中暑和抗精神病药恶性综合征中，芽状体核作为严重高温和全身炎症的生物标志物的诊断价值：气候变化的挑战。

International journal of laboratory hematology Pub Date : 2025-05-20 DOI: 10.1111/ijlh.14500

Gonzalo Verdú, Marina Vallvé, Paula San-José, Angel Molina, Anna Merino

{"title":"Diagnostic Value of Botryoid Nuclei as a Biomarker of Severe Hyperthermia and Systemic Inflammation in Heatstroke and Neuroleptic Malignant Syndrome: A Challenge of Climate Change.","authors":"Gonzalo Verdú, Marina Vallvé, Paula San-José, Angel Molina, Anna Merino","doi":"10.1111/ijlh.14500","DOIUrl":"https://doi.org/10.1111/ijlh.14500","url":null,"abstract":"Introduction: With the increasing frequency of heat waves due to climate change, heat-related illnesses are becoming more common. Heatstroke is a life-threatening condition characterized by hyperthermia and multiple organ failure. A rare morphological feature, botryoid nuclei, has been identified in the peripheral blood of patients with hyperthermia, but its significance is not well understood. This study investigates the diagnostic value of botryoid neutrophils as a potential marker of severe hyperthermia and systemic inflammation in heatstroke and neuroleptic malignant syndrome.Methods: We performed a retrospective analysis of six patients with hyperthermia who were admitted to the Hospital Clínic of Barcelona and the Hospital de la Santa Creu i Sant Pau during the summers of 2023 and 2024. Peripheral blood smears were analyzed using CellaVision DM9600, MC-80, and DI60 analyzers. Blood cell counts were obtained using Advia 2120i and Sysmex XN analyzers. Biochemical parameters were measured using Atellica Solutions and Alinity analyzers.Results: Among the six cases, five were diagnosed with heatstroke and one with neuroleptic malignant syndrome. All patients exhibited botryoid nuclei in leucocytes (8% to 29%), more frequently observed in neutrophils. Hematologic findings included leucocytosis, neutrophilia with left shift, and thrombocytopenia. Biochemical analysis revealed significant organ dysfunction, including elevated liver enzymes, renal failure, and increased inflammatory markers, such as procalcitonin and lactic acid.Conclusion: Botryoid-nuclei leucocytes may serve as a diagnostic marker for hyperthermia-related conditions, including heatstroke and neuroleptic malignant syndrome. This finding underscores the relevance of early recognition and intervention in patients presenting with hyperthermia and systemic inflammation.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144113136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ring Chromosomes 7 in Two Cases of Myelodysplastic Syndrome: Isolated Abnormality and Complex Karyotype Including idic(21)(p11.2) Duplication. 2例骨髓增生异常综合征的7号环染色体：孤立异常和复杂核型，包括idic(21)（p11.2）重复。

International journal of laboratory hematology Pub Date : 2025-05-19 DOI: 10.1111/ijlh.14499

Katsuya Yamamoto, Keiji Kurata, Kimikazu Yakushijin, Hironobu Minami

引用次数: 0

Response to Dr. Sorigue's Letter Regarding Our Article: "A New Diagnostic Approach for Myelodysplastic Neoplasms Using a Combination of Scores Based on Flow Cytometry and Automated Hematology Sysmex XN Analyzers". 回复Sorigue博士关于我们的文章：“使用基于流式细胞术和自动血液学Sysmex XN分析仪的评分组合诊断骨髓增生异常肿瘤的新方法”的来信。

International journal of laboratory hematology Pub Date : 2025-05-19 DOI: 10.1111/ijlh.14496

Ludovic Firrera, Benjamin Podvin, Julien Herlem, Marion Magierowicz, Alexandre Willaume, Vincent Thibaud, Agnès Charpentier

引用次数: 0

Lupus Anticoagulant Testing in Challenging Situations: Navigating Complexities. 狼疮抗凝血试验在挑战性的情况下：导航复杂性。

International journal of laboratory hematology Pub Date : 2025-05-16 DOI: 10.1111/ijlh.14490

Katrien M J Devreese

{"title":"Lupus Anticoagulant Testing in Challenging Situations: Navigating Complexities.","authors":"Katrien M J Devreese","doi":"10.1111/ijlh.14490","DOIUrl":"https://doi.org/10.1111/ijlh.14490","url":null,"abstract":"The laboratory diagnosis of antiphospholipid syndrome (APS) relies on detecting persistent antiphospholipid antibodies (aPL), including lupus anticoagulant (LA) as one of the three laboratory criteria. LA is a well-established risk factor in APS and the cornerstone of APS diagnosis, identifying triple-positive patients. LA testing is supported by testing guidelines, but challenges remain with LA measurement, which is complex and susceptible to numerous pitfalls and interferences. A nuanced understanding of the technical and interpretative aspects of LA testing is crucial for accurate diagnosis. Limited to specific patient populations, the timing of testing in relation to clinical events is essential to avoid false results, and only persistent LA positivity is considered diagnostic. Proper sample preparation, choice of assays, test procedure, cutoff values, and strategies to handle anticoagulant therapy interference pose significant challenges in LA testing. While advances in the harmonization of test procedures and testing guidelines have improved diagnostic reliability, the complex nature of LA demands careful consideration of methodological issues and potential interferences, underscoring the importance of expertise and interdisciplinary communication in achieving accurate results. Interpretative comments and close interaction between clinical pathologists and clinicians are mandatory for diagnosis and patient management. This paper will focus on the technical aspects of the laboratory method and the interpretation of LA results in the diagnosis of APS with attention to specific situations where LA testing is difficult.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-Dependent EDTA Effect on Leukocyte Differential Count and Morphology Compared to Blood Smear Made by Direct Finger Prick. EDTA对直接手指刺血涂片白细胞计数和形态学的影响。

International journal of laboratory hematology Pub Date : 2025-05-16 DOI: 10.1111/ijlh.14498

Anna Irding, Filip Landgren, Elisabeth Aardal

{"title":"Time-Dependent EDTA Effect on Leukocyte Differential Count and Morphology Compared to Blood Smear Made by Direct Finger Prick.","authors":"Anna Irding, Filip Landgren, Elisabeth Aardal","doi":"10.1111/ijlh.14498","DOIUrl":"https://doi.org/10.1111/ijlh.14498","url":null,"abstract":"Introduction: The impact of ethylenediaminetetraacetic acid (EDTA) on leukocyte count and morphology has led to the current recommendation that smears made from EDTA blood should be prepared within 4 h of sampling. However, previous studies have only been performed on smears with normal cell counts, and smears made from K2EDTA blood have not been studied in comparison with smears made from direct finger prick. The aim of this study was to investigate the time limit for performing a morphological assessment on smears from EDTA blood without compromising the results.Methods: Blood samples from 37 patients with known or suspected abnormal leukocyte morphology were selected, with smears from direct finger prick used as controls. Smears were prepared from the original EDTA tube every 4 h up to 24 h after sampling. The smears were evaluated for differential count, morphological assessment, and smear quality scoring.Results: No significant difference in cell count was observed on smears made up to 12 h after sampling for neutrophils (p = 0.430), lymphocytes (p = 0.080), monocytes (p = 0.948) eosinophils (p = 0.398), basophils (p = 0.460), and blast cells (p = 0.239) compared to smears made by finger prick. However, smears prepared later than 8 h postsampling showed a significant increase in morphological changes.Conclusion: A manual differential leukocyte count can be reliably performed on smears from EDTA blood within 12 h, whereas a comprehensive morphological assessment requires smears to be prepared within 8 h of sampling.","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0