International journal of laboratory hematology最新文献

{"title":"Analytical and Clinical Performance of the Atellica HEMA 580 Haematology Analyzer: A Comparison With the ADVIA 2120i.","authors":"Hyeongyu Lee, Jooyoung Cho, Gilsung Yoo, Juwon Kim","doi":"10.1111/ijlh.70107","DOIUrl":"10.1111/ijlh.70107","url":null,"abstract":"<p><strong>Introduction: </strong>The Atellica HEMA 580 is a novel haematology analyser providing eight-part leukocyte differential and optical platelet (PLT) counting. We compared its analytical and clinical performance with that of the ADVIA 2120i.</p><p><strong>Methods: </strong>A total of 796 residual whole blood specimens were analysed. Carryover, linearity and precision were assessed. Inter-instrument comparison was performed for complete blood counts and five-part differential (5-Diff) parameters using Passing-Bablok regression and Bland-Altman analyses. Additional regression against manual microscopy was conducted for flagged samples, including PLT, reticulocytes and common 5-Diff parameters.</p><p><strong>Results: </strong>Carryover was < 1% for all parameters. Linearity was excellent (R² ≥ 0.99). Precision was within acceptable limits; coefficient of variant was < 5% for most parameters, except optical PLT, lymphocytes, monocytes, eosinophils and nucleated red blood cells under specific conditions. Inter-instrument comparisons revealed remarkably high correlations (r ≥ 0.9) for most parameters, whereas mean corpuscular haemoglobin concentration (MCHC) (r = 0.429) and basophils (r = 0.627) showed weaker concordance. Passing-Bablok regression indicated minimal systematic or proportional bias for most parameters. Bland-Altman analysis showed that > 95% of results were within the 95% limits of agreement, except white blood cells, mean corpuscular volume and PLT (94.7%-94.8%). Comparison with microscopy confirmed good concordance for PLT and reticulocytes; however, lymphocytes and monocytes had proportional and systematic biases.</p><p><strong>Conclusion: </strong>The Atellica HEMA 580 demonstrated reliable analytical performance and strong agreement with the ADVIA 2120i. This supports its suitability as a routine haematology analyser, providing accurate and clinically comparable results.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"784-794"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147655134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immature Platelet Fraction and Absolute Immature Platelet Count as Diagnostic Markers in Pediatric Immune Thrombocytopenia.","authors":"Fernanda Gomes Nascimento, Karla Nunes Pereira, Laura Eduarda de Oliveira, Ighor Seiji Okumura Tioda, Cecília de Farias Dutra, Rafael Noal Moresco, Clóvis Paniz, José Antonio Mainardi de Carvalho","doi":"10.1111/ijlh.70088","DOIUrl":"10.1111/ijlh.70088","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"883-886"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-Acetylornithine Depletion in Bone Marrow Biopsy: A Novel Microenvironment-Specific Hallmark for Multiple Myeloma Diagnosis.","authors":"Bingjie Wang, Shuanglian Xie, Weiwei Xie, Jialin Zhu, Bo Tang, Ye Shen, Zeyin Liang, Wei Liu, Zhizhen Lai, Yujun Dong","doi":"10.1111/ijlh.70116","DOIUrl":"10.1111/ijlh.70116","url":null,"abstract":"<p><strong>Introduction: </strong>The bone marrow microenvironment plays crucial roles in the pathogenesis of multiple myeloma. Previous studies have shown that there are differences in the metabolomics of bone marrow suspension and plasma, but there is rare research on bone marrow biopsy tissue. This study aims to establish a new metabolomics approach to analyze bone marrow biopsy tissues and explore its diagnostic value.</p><p><strong>Methods: </strong>We collected bone marrow biopsy tissues from 19 newly-diagnosed multiple myeloma(MM), 29 monoclonal gammaglobulinemia of undetermined significance(MGUS) and 30 lymphoma without bone marrow invasion as the controls. Metabolomics analysis was conducted using high-performance liquid chromatography-mass spectrometry. Diagnostic Biomarkers and associated pathway were analyzed.</p><p><strong>Results: </strong>The metabolic profiles differed between the controls and MGUS or MM. For MGUS versus MM comparison, Phenylalanyl-Valine and N-Acetylornithine were significantly down-regulated in the MM while N-Acetylornithine has the best diagnostic value with the sensitivity and specificity of 87.5% and 71.4%. For the controls vs. MM comparison, N-Acetylornithine and Aspartyl-Arginine differed markedly. Compared with the controls group, N-Acetylornithine was significantly down-regulated in the MM group, and the AUC of N-Acetylornithine for MM was 0.933, with sensitivity and specificity of (90.0% and 93.3%). N-Acetylornithine displayed the same trends as the comparison between MGUS and MM. Furthermore, when comparing MGUS and MM, Arginine biosynthesis was identified as significantly disrupted in which N-Acetylornithine was involved.</p><p><strong>Conclusions: </strong>We establish a novel method for metabolomic profiling of bone marrow biopsy tissue and provide evidence of metabolic alterations, particularly reduced N-acetylornithine levels, in MGUS and MM patients.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"839-847"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147719278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of Red Blood Cells From Sickle Cell Disease Patients for Comparison of Oxygen Gradient Ektacytometry Parameters Between Laboratories to Enable Data Harmonization.","authors":"Marissa J M Traets, Amira Idrizovic, Jennifer Eijkelenboom-Bos, Martijn Veldthuis, Menno van der Hoorn, Myrthe J van Dijk, Romee Balvert, Mirco D'Agnolo, Mickaël Marin, Emmanuelle Bourdelier, Emmanuel Adu, Nicolas Hebert, Pablo Bartolucci, Maria Paola Boaro, Mariane de Montalembert, Raffaella Colombatti, Vivien A Sheehan, Eduard J van Beers, Richard van Wijk, María Del Mar Mañú-Pereira, Rob van Zwieten, Minke A E Rab","doi":"10.1111/ijlh.70094","DOIUrl":"10.1111/ijlh.70094","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"900-905"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147489040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Value of CD56 and CD200 Co-Expression by Flow Cytometry in Neuroendocrine Carcinomas.","authors":"Radu Chiriac, Emma Beausoleil, Tanguy Fenouil, Marie Donzel, Lucile Baseggio","doi":"10.1111/ijlh.70099","DOIUrl":"10.1111/ijlh.70099","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"906-910"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147505735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk Factors of Disseminated Tumor Cells in the Bone Marrow of Patients With Angioimmunoblastic T-Cell Lymphoma and Their Impact on Prognosis.","authors":"Dongyao Yan, Xueyan Cao, Xiaoqian Wang, Baohong Yue","doi":"10.1111/ijlh.70112","DOIUrl":"10.1111/ijlh.70112","url":null,"abstract":"<p><strong>Introduction: </strong>Angioimmunoblastic T-cell lymphoma (AITL) is a mature T-cell proliferative tumor characterized by an aggressive clinical course and low incidence. The risk factors for the presence of disseminated tumor cells (DTCs) in the bone marrow (BM) of patients and their impact on prognosis remain incompletely understood. In this study, we used multiparametric flow cytometry (MFC) to detect DTCs in the BM of AITL patients. We aimed to assess risk factors for the presence of DTCs, investigate correlations between the immunophenotype of DTCs and clinical indicators or efficacy, and identify prognostic factors. Our findings provide insights that may aid in the early diagnosis, disease monitoring, and efficacy evaluation of AITL.</p><p><strong>Methods: </strong>Based on the presence or absence of DTCs in the BM, 90 patients with an initial diagnosis of AITL were classified into DTCs⁺ and DTCs^- groups. The differences between the two groups were then compared. Clinical and survival data were collected from AITL patients to analyze the correlations between immunophenotypes and clinical characteristics or prognosis. Univariate logistic regression was employed to analyze the factors influencing efficacy.</p><p><strong>Results: </strong>MFC detected neoplastic T cells of AITL in 65.56% (59/90) of BM samples. Compared to the DTCs^- patients, DTCs⁺ patients had a higher frequency of fever, splenomegaly, extranodal involvement ≥ 2 sites, and elevated lactate dehydrogenase (LDH). The 2-year progression-free survival (PFS) rates were 31.3% and 52.5%, and the 2-year overall survival (OS) rates were 65.6% and 65.3% in the DTCs⁺ and DTCs^- groups, respectively. Multivariate survival analysis demonstrated that pleural effusion was a risk factor affecting PFS and OS. Furthermore, loss of CD7 expression on DTCs was associated with shorter OS. AITL patients with CD7 loss were more likely to present with rash, elevated immunoglobulins, and CXCL13 loss by histopathological immunohistochemistry. Finally, CD3 expression, elevated LDH, and decreased hemoglobin (HGB) were collectively identified as high-risk factors for disease progression.</p><p><strong>Conclusion: </strong>The presence of DTCs in the BM is associated with the prognosis of AITL. Pleural effusion constitutes an independent adverse prognostic factor. Loss of CD7 correlates with shorter OS, and a profile combining CD3 expression, elevated LDH, and decreased HGB serves as a useful predictor of disease progression.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"823-830"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147648028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concordance Between Fragment Analysis and Next-Generation Sequencing in Identification of Non-Standard CALR Indels.","authors":"Juan Luis Gomez Marti, Neha Seth, Ahmed Bendari, Wayne Tam, Sylvia Spitzer, Oana C Rosca, Oana Vele","doi":"10.1111/ijlh.70093","DOIUrl":"10.1111/ijlh.70093","url":null,"abstract":"<p><strong>Objectives: </strong>Identification of CALR indels is key in the diagnosis of myeloproliferative neoplasms (MPNs) that are JAK2 or MPL wild-type. Most patients harbor either type 1 (52-bp deletion) or type 2 (5-bp insertion) exon 9 indels, both of which produce a +1 frameshift and loss of the KDEL ER-retention motif. Additional indels have been characterized. However, many still present diagnostic challenges in interpretation, especially when relying on fragment size alone. Our goal was to assess the concordance of detection of rare CALR indels using two available molecular diagnostic assays in the diagnosis of MPN.</p><p><strong>Methods: </strong>We reviewed 6 years of CALR exon 9 testing at our institution, focusing on patients with non-type 1/type 2 indels (non-standard). Fragment sizing was performed by PCR with capillary electrophoresis (PCR-CE), with a subset reflexed to next-generation sequencing (NGS) for clarification. We assessed reading frame shifts, KDEL motif status, variant allele frequencies (VAFs), and co-occurring mutations where available.</p><p><strong>Results: </strong>Over a 6-year period, a total of 3474 CALR tests were performed, with indels being detected in 125 patients. Forty patients with exon 9 CALR indels other than 52 bp-del and 5 bp-ins were identified (40/125; 32%). Among these, the most frequently detected indel was the 9-bp deletion (19/125; 15.2%). Parallel NGS reports were available in 11 patients. From these, five showed a discrepant indel size (5/11 = 45%) between PCR-CE and NGS by ±1 or -2-bp. These discrepancies resulted in novel frameshifts that were originally categorized as in-frame indels by PCR-CE.</p><p><strong>Conclusions: </strong>PCR-CE should be used as a screening method, and indels other than 52-bp del or 5-bp ins should prompt reflex NGS testing to determine frame and clonal status. These findings establish an opportunity to standardize molecular testing for CALR testing in patients with suspected MPN.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"807-815"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147489075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Genetic Characterization of 269 Patients With Suspected Inherited Platelet Disorders: The Padua Monocentric Experience.","authors":"Silvia Ferrari, Daniela Regazzo, Antonella Bertomoro, Anna Cerbo, Alessandro Galbiati, Elisabetta Cosi, Paolo Simioni","doi":"10.1111/ijlh.70081","DOIUrl":"10.1111/ijlh.70081","url":null,"abstract":"<p><strong>Background: </strong>Inherited platelet disorders (IPDs) are rare hematologic conditions encompassing a heterogeneous spectrum of quantitative and qualitative platelet defects, frequently associated with variable clinical phenotypes and comorbidities. Accurate diagnosis necessitates comprehensive genetic characterization, detailed clinical and bleeding history, and systematic evaluation of platelet count, size, and function, which are essential to distinguish IPDs from immune thrombocytopenia (ITP).</p><p><strong>Aim: </strong>The aim of this study was to clinically and genetically characterize a total of 269 patients from 140 families with platelet disorders, evaluated at the First Chair, Department of Medicine, University of Padua.</p><p><strong>Methods: </strong>Patients with suspected platelet disorders underwent comprehensive laboratory evaluation, and Sanger sequencing was performed to identify causative variants in approximately 30% of cases.</p><p><strong>Results: </strong>We identified 80 patients from 44 families who were found to carry pathogenic or likely pathogenic variants, with the most frequent ones being linked to Bernard-Soulier syndrome (BSS), characterized by thrombocytopenia and bleeding tendency. Additionally, 23 subjects had MYH9 gene variants and one patient was affected by Glanzmann thrombasthenia. We also discovered seven pathogenic variants not previously described in the literature.</p><p><strong>Conclusion: </strong>Due to the limitations of the Sanger method, the molecular defect could only be defined in approximately 30% of cases. In the remaining 70% of patients, the genetic cause remained unidentified, highlighting the need for further evaluation using an extended NGS panel targeting genes associated with inherited thrombocytopenias.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"856-865"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147277749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-Mimicking RBC Microfragments: PLT-F Interference on Sysmex XN Analyzers in Pediatrics.","authors":"Anastasia Milojkovic, Odile Fenneteau, Anne Vincenot, Aurélie Galimard, Elodie Lainey","doi":"10.1111/ijlh.70087","DOIUrl":"10.1111/ijlh.70087","url":null,"abstract":"","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"879-882"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utility of Iron Staining in Bone Marrow Aspirates in the Era of Next-Generation Sequencing: A Retrospective Single-Centre Study.","authors":"Vibooshini Ganeshalingam, Kirollos Kamel","doi":"10.1111/ijlh.70114","DOIUrl":"10.1111/ijlh.70114","url":null,"abstract":"<p><strong>Introduction: </strong>Bone marrow iron staining is traditionally used to assess iron stores and identify ring sideroblasts (RS), a key feature of sideroblastic anaemia and historically important in the classification of myelodysplastic and myelodysplastic/myeloproliferative overlap neoplasms. With the adoption of the WHO 2022 classification and increasing availability of next-generation sequencing (NGS), the diagnostic relevance of routine iron staining warrants re-evaluation.</p><p><strong>Methods: </strong>We retrospectively analysed 101 consecutive adult diagnostic bone marrow examinations (51 myeloid, 50 lymphoid) performed at a regional Australian hospital between January 2023 and June 2024. Perls' Prussian blue staining was routinely performed. Ferritin was available in 76% of cases, and molecular testing in 35% of cases. Two independent reviewers graded bone marrow iron using the Gale score and enumerated RS. Diagnostic impact was categorised as either no impact, supportive but non-essential, or potential diagnostic miss. Interobserver agreement, correlation with serum ferritin, and diagnostic performance for iron deficiency were evaluated.</p><p><strong>Results: </strong>Iron staining had no diagnostic impact in 94% of cases and was supportive but non-essential in 6%; omission would not have altered the final diagnosis in any case. RS ≥ 15% were identified in seven myeloid cases, including MDS, AML, and therapy-related myeloid neoplasms. SF3B1 mutations were present in three RS-positive cases; RS presence did not independently determine diagnosis. Interobserver agreement for iron grading was good (weighted κ = 0.77, 95% CI 0.64-0.89). Correlation between marrow iron grade and serum ferritin was weak (r ≈ 0.38). Low marrow iron grade (≤ 1) showed variable sensitivity (33% to 67%) but high specificity (> 90%) for iron deficiency.</p><p><strong>Conclusion: </strong>Routine bone marrow iron staining rarely influences diagnostic classification in contemporary practice. RS morphology is supportive but not determinative, and marrow iron grading correlates poorly with systemic iron status. These data support a targeted rather than routine approach to marrow iron staining, particularly where NGS is available and routinely performed in de novo diagnoses.</p>","PeriodicalId":94050,"journal":{"name":"International journal of laboratory hematology","volume":" ","pages":"831-838"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13357893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147679673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}