使用迈瑞BC-6200、流式细胞术和手工显微镜检测网织红细胞计数的参考区间和比较分析。

IF 2.2 4区医学 Q3 HEMATOLOGY

International Journal of Laboratory Hematology Pub Date : 2025-02-06 DOI:10.1111/ijlh.14438

Hathaiphon Aphaiwiwat, Chutitorn Ketloy, Tapakorn Thepnarin, Phandee Watanaboonyongcharoen, Eakachai Prompetchara

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引用次数: 0

摘要

本研究评估了迈瑞BC-6200网织红细胞计数分析仪的分析性能，重点是不精确性、结转性和时间稳定性。将网织细胞计数的准确性与手工显微镜（MM）和参考方法流式细胞术（FC）进行比较。此外，为泰国人群建立了网织红细胞计数的参考区间（RIs）和相关参数。方法：选择60名健康的泰国男女成年人以及182份来自不同病理状况个体的剩余血液样本，使用自动迈瑞BC-6200评估网织网细胞计数。将结果与MM和FC进行比较，建立RIs并评估方法之间的相关性。结果：网织红细胞计数的不精确性在所有控制水平（变异系数，%CV）低于制造商的声明。结转率9/L: 34.88-118.50，未成熟网织红细胞分数（IRF）（%）： 2.71-15.28，网织红细胞血红蛋白含量（RHE）（pg）： 22.84-29.70。网织红细胞计数在自动分析仪、MM和FC之间有很强的相关性（r > 0.9785）。结论：迈瑞BC-6200是一种可靠的网红细胞计数替代MM和FC方法，具有良好的相关性、精度、低携带性和时间稳定性，可用于临床评估红细胞生成。然而，需要进一步的研究来评估网织红细胞计数和相关参数在诊断血液学疾病中的临床应用。

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Reference Intervals and Comparative Analysis of Reticulocyte Counts Using the Mindray BC-6200, Flow Cytometry, and Manual Microscopy

Introduction

This study evaluated the analytical performance of the Mindray BC-6200 analyzer for reticulocyte counting, with a focus on imprecision, carryover, and time stability. The accuracy of reticulocyte count was compared with the manual microscope (MM) and flow cytometry (FC), the reference method. Additionally, reference intervals (RIs) of reticulocyte count and related parameters were established for the Thai population.

Methods

Sixty healthy Thai adults of both sexes along with 182 leftover blood samples from individuals with various pathologic conditions, were selected to evaluate reticulocyte counts using the automated Mindray BC-6200. Results were compared with MM and FC to establish RIs and assess the correlation between methods.

Results

The imprecision on reticulocyte counts across all control levels (coefficient of variation, %CV) was below the manufacturer's claim. Carryover was < 0.001%, and time-stability was excellent up to 24 h. RIs were as follows: reticulocytes (×10⁹/L): 34.88–118.50, immature reticulocyte fraction (IRF) (%): 2.71–15.28, reticulocyte Hemoglobin content (RHE) (pg): 22.84–29.70. There was a strong correlation (r > 0.9785) in reticulocyte counts between the automated analyzer, MM, and FC.

Conclusion

The Mindray BC-6200 is a reliable alternative to MM and FC methods for reticulocyte counting, with a good correlation, precision, low carryover, and time stability, making it effective for assessing erythropoiesis in clinical settings. However, further studies are needed to evaluate the clinical utility of reticulocyte count and related parameters in diagnosing hematological conditions.

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.