Ibrain Pub Date : 2023-09-10 DOI: 10.1002/ibra.12131

Ke-Qian Liu, Xue Bai, Ji-Lin Chen, Guo-Jiao Chen, Muhammad Ameen Jamal, Yu-Qi He

{"title":"Molecular network mechanism of Shexiang Huayu Xingnao granules in treating intracerebral hemorrhage","authors":"Ke-Qian Liu, Xue Bai, Ji-Lin Chen, Guo-Jiao Chen, Muhammad Ameen Jamal, Yu-Qi He","doi":"10.1002/ibra.12131","DOIUrl":"10.1002/ibra.12131","url":null,"abstract":"<p>We aim to explore the pharmacological efficacy and molecular network mechanism of Shexiang Huayu Xingnao granules (SX granules) in the treatment of intracerebral hemorrhage (ICH) based on experiments and network pharmacology. After the ICH model establishment, the behavioral functions of rats were assessed by the modified neurological severity score (mNSS), the wire suspension test, and the rotarod test. Brain histomorphological changes were observed using 2,3,5-triphenyl tetrazolium chloride (TTC), hematoxylin–eosin (HE), Nissl, and TdT-mediated dUTP nick end labeling (TUNEL) combined with neuronal nuclear (NEUN) immunofluorescence staining. The cross-targets of SX granules and ICH were obtained using network pharmacology, gene ontology (GO) enrichment analysis, and Kyoto encyclopedia of genes and genomes (KEGG) signaling pathway analysis were performed. Then, the obtained Hub genes were verified using real-time quantitative polymerase chain reaction (RT-qPCR). The mNSS score was reduced and the duration to remain wire suspended increased in the SX group. In the morphological experiment, SX granules reduced brain tissue damage, neuronal apoptosis, and the number of astrocytes in the ICH rats. Moreover, 607 targets of drug–disease intersection were obtained by network pharmacology, and 10 Hub genes were found. SX granules regulated the expression of HRAS, MAPK3, and STAT3 in ICH condition. In conclusion, SX granules improved behavioral dysfunction, abnormal alterations in brain tissue, and cell morphology in ICH rats, and potential molecular mechanism was linked with the expression of multiple genes.</p>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136072251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt signaling in synaptogenesis of Alzheimer's disease Wnt信号在阿尔茨海默病突触发生中的作用。

Ibrain Pub Date : 2023-09-06 DOI: 10.1002/ibra.12130

Cheng-Ting Zhang, Joy Wang, Wen-Yuan Wang

{"title":"Wnt signaling in synaptogenesis of Alzheimer's disease","authors":"Cheng-Ting Zhang, Joy Wang, Wen-Yuan Wang","doi":"10.1002/ibra.12130","DOIUrl":"10.1002/ibra.12130","url":null,"abstract":"<p>Alzheimer's disease (AD), recognized as the leading cause of dementia, occupies a prominent position on the list of significant neurodegenerative disorders, representing a significant global health concern with far-reaching implications at both individual and societal levels. The primary symptom of Alzheimer's disease is a decrease in synaptic potency along with synaptic connection loss. Synapses, which act as important linkages between neuronal units within the cerebral region, are critical in signal transduction processes essential to orchestrating cognitive tasks. Synaptic connections act as critical interconnections between neuronal cells inside the cerebral environment, facilitating critical signal transduction processes required for cognitive functions. The confluence of axonal and dendritic filopodial extensions culminates in the creation of intercellular connections, coordinated by various signals and molecular mechanisms. The progression of synaptic maturation and plasticity is a critical determinant in maintaining mental well-being, and abnormalities in these processes have been linked to the development of neurodegenerative diseases. Wnt signaling pathways are important to the orchestration of synapse development. This review examines the complicated interplay between Wnt signaling and dendritic filopodia, including an examination of the regulatory complexities and molecular machinations involved in synaptogenesis progression. Then, these findings are contextualized within the context of AD pathology, allowing for the consideration of prospective therapeutic approaches based on the findings and development of novel avenues for future scientific research.</p>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41173020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the treatment and prevention of peripheral neuropathy after multiple myeloma treatment 治疗和预防多发性骨髓瘤治疗后周围神经病变的最新进展

Ibrain Pub Date : 2023-09-06 DOI: 10.1002/ibra.12132

Dan Wen, Song Cao, Yonghuai Feng

引用次数: 0

The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain 大麻素CB1受体在周围神经性疼痛大鼠间充质干细胞的镇痛和修复作用中的作用。

Ibrain Pub Date : 2023-08-24 DOI: 10.1002/ibra.12129

Anna-Maria V. Yerofeyeva, Sergey V. Pinchuk, Svetlana N. Rjabceva, Alla Y. Molchanova

{"title":"The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain","authors":"Anna-Maria V. Yerofeyeva, Sergey V. Pinchuk, Svetlana N. Rjabceva, Alla Y. Molchanova","doi":"10.1002/ibra.12129","DOIUrl":"10.1002/ibra.12129","url":null,"abstract":"<p>Mesenchymal stem cells (MSCs) can produce antinociceptive and reparative effects. Presumably, the MSCs-induced antinociception may be partly due to the involvement of the endocannabinoid system. The study aimed to evaluate the antinociceptive and reparative effects of adipose-derived MSCs (ADMSCs) upon pharmacological modulation of cannabinoid CB<sub>1</sub> receptor in peripheral tissues or on ADMSCs' membranes in a rat model of peripheral neuropathy. ADMSCs were injected into the area of rat sciatic nerve injury (i) with no additional treatments, (ii) at the tissue CB<sub>1</sub> receptor activation by endogenous agonist anandamide (AEA) or blockade with a selective AM251 antagonist; and (iii) preincubated with AEA or AM251. The evaluation of CB<sub>1</sub> receptor activity involved analyzing nociceptive responses, gait parameters, and histology. Transplantation of ADMSCs upon activation of CB<sub>1</sub> receptors, both on AMSCs' membranes or in the area of nerve injury, accelerated the analgesia and recovery of dynamic gait parameters, abolished static gait disturbances, and promoted the fastest nerve regeneration. Only blockade of CB<sub>1</sub> receptors on ADMSCs shortened ADMSCs-induced analgesia and decreased the number of preserved nerve fibers. CB<sub>1</sub> receptors on ADMSCs significantly contribute to their pain-relieving and tissue-repairing capabilities by stimulating the growth factors secretion and suppressing the release of pro-inflammatory cytokines. Peripheral CB<sub>1</sub> receptors do not significantly influence ADMSC-induced antinociception.</p>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41161256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CCA repair or ECA ligation—Which middle cerebral artery occlusion is better in the reperfusion mouse model? CCA修复还是ECA结扎在再灌注小鼠模型中哪种大脑中动脉闭塞更好？

Ibrain Pub Date : 2023-08-18 DOI: 10.1002/ibra.12128

Yue Hu, Zhen-Hong Yang, Feng Yan, Shuang-Feng Huang, Rong-Liang Wang, Zi-Ping Han, Jun-Fen Fan, Yang-Min Zheng, Ping Liu, Yu-Min Luo, Si-Jie Li

{"title":"CCA repair or ECA ligation—Which middle cerebral artery occlusion is better in the reperfusion mouse model?","authors":"Yue Hu, Zhen-Hong Yang, Feng Yan, Shuang-Feng Huang, Rong-Liang Wang, Zi-Ping Han, Jun-Fen Fan, Yang-Min Zheng, Ping Liu, Yu-Min Luo, Si-Jie Li","doi":"10.1002/ibra.12128","DOIUrl":"10.1002/ibra.12128","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>A reliable animal model is essential for ischemic stroke research. The implications of the external carotid artery (ECA) transection or common carotid artery (CCA) ligation have been described. Thus, a modified animal model, the CCA-repair model, has been established, and studies have shown that the CCA-repair model has potential advantages over the CCA-ligation model. However, whether the CCA-repair model is superior to the ECA-ligation model remains unclear. Sixty male C57BL/6 mice were randomly assigned to establish the CCA-repair (<i>n</i> = 34) or ECA-ligation (<i>n</i> = 26) models. Cerebral blood flow before middle cerebral artery occlusion (MCAO), immediately after MCAO and reperfusion were monitored and the operation duration, postoperative body weight, and food intake within 7 days, and the number of intraoperative and postoperative deaths within 7 days were recorded in the two models. Modified neurological severity scores and Bederson (0–5) scores were used to evaluate postoperative neurological function deficits on Days 1/3/5/7. 2,3,5-Triphenyltetrazolium chloride staining was used to quantify lesion volume on Day 7 after the operation. We found the establishment of the CCA-repair model required a longer total operation duration (<i>p</i> = 0.0175), especially the operation duration of reperfusion (<i>p</i> < 0.0001). However, there was no significant difference in body weight and food intake development, lesion volume and intragroup variability, neurological function deficits, mortality, and survival probability between the two groups. The CCA-repair model has no significant advantage over the ECA-ligation model. The ECA-ligation model is still a better choice for focal cerebral ischemia.</p>\u0000 </section>\u0000 </div>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12128","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41160621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of Robo1 in the pentylenetetrazol-kindled epilepsy mouse model Robo1 在戊四唑诱发癫痫小鼠模型中的作用机制

Ibrain Pub Date : 2023-08-15 DOI: 10.1002/ibra.12127

Zheng Liu, Wei Huang, Man-Min Zhu, Zhong-Xiang Xu, Zu-Cai Xu, Chang-Yin Yu, Hao Huang

{"title":"Mechanism of Robo1 in the pentylenetetrazol-kindled epilepsy mouse model","authors":"Zheng Liu, Wei Huang, Man-Min Zhu, Zhong-Xiang Xu, Zu-Cai Xu, Chang-Yin Yu, Hao Huang","doi":"10.1002/ibra.12127","DOIUrl":"10.1002/ibra.12127","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>The neural network hypothesis is one of the important pathogenesis of drug-resistant epilepsy. Axons guide molecules through synaptic remodeling and brain tissue remodeling, which may result in the formation of abnormal neural networks. Therefore, axon guidance plays a crucial role in disease progression. However, although Robo1 is one of the important components of axon guidance, the role of Robo1 in epilepsy remains unclear. In this study, we aimed to explore the mechanism of Robo1 in epilepsy. Male adult C57BL/6 mice were intraperitoneally injected with pentylenetetrazol to establish an epilepsy model. Lentivirus (LV) was given via intracranial injection 2 weeks before pentylenetetrazol injection. Different expressions of Robo1 between the control group, LV-mediated Robo1 short hairpin RNA group, empty vector control LV group, and normal saline group were analyzed using Western blot, immunofluorescence staining, Golgi staining, and video monitoring. Robo1 was increased in the hippocampus in the pentylenetetrazol-induced epilepsy mouse model; lentiviral Robo1 knockdown prolonged the latency of seizure and reduced the seizure grade in mice and resulted in a decrease in dendritic spine density, while the number of mature dendritic spines was maintained. We speculate that Robo1 has been implicated in the development and progression of epilepsy through its effects on dendritic spine morphology and density. Epileptic mice with Robo1 knockdown virus intervention had lower seizure grade and longer latency. Follow-up findings suggest that Robo1 may modulate seizures by affecting dendritic spine density and morphology. Downregulation of Robo1 may negatively regulate epileptogenesis by decreasing the density of dendritic spines and maintaining a greater number of mature dendritic spines.</p>\u0000 </section>\u0000 </div>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81339762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress of postoperative cognitive dysfunction in cardiac surgery under cardiopulmonary bypass 心肺旁路下心脏手术术后认知功能障碍的研究进展

Ibrain Pub Date : 2023-08-14 DOI: 10.1002/ibra.12123

Yi-Ming Zhuang, Ji-Yang Xu, Kun Zheng, Hong Zhang

{"title":"Research progress of postoperative cognitive dysfunction in cardiac surgery under cardiopulmonary bypass","authors":"Yi-Ming Zhuang, Ji-Yang Xu, Kun Zheng, Hong Zhang","doi":"10.1002/ibra.12123","DOIUrl":"10.1002/ibra.12123","url":null,"abstract":"<p>Cardiopulmonary bypass (CPB) is often used in cardiothoracic surgery because its nonphysiological state causes pathophysiological changes in the body, causing multiorgan and multitissue damage to varying degrees. Postoperative cognitive dysfunction (POCD) is a common central nervous system complication after cardiac surgery. The etiology and mechanism of POCD are not clear. Neuroinflammation, brain mitochondrial dysfunction, cerebral embolism, ischemia, hypoxia, and other factors are related to the pathogenesis of POCD. There is a close relationship between CPB and POCD, as CPB can cause inflammation, hypoxia and reperfusion injury, and microemboli formation, all of which can trigger POCD. POCD increases medical costs, seriously affects patients' quality of life, and increases mortality. Currently, there is a lack of effective treatment methods for POCD. Commonly used methods include preoperative health management, reducing inflammation response during surgery, preventing microemboli formation, and implementing individualized rehabilitation programs after surgery. Strengthening preventive measures can minimize the occurrence of POCD and its adverse effects.</p>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75458583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Double gene mutations of LRSAM1 and REEP1 and a new REEP1 mutation site found in a patient with amyotrophic lateral sclerosis with subjective paresthesia: A case report LRSAM1和REEP1双基因突变和一个新的REEP1突变位点在肌萎缩侧索硬化症伴有主观感觉异常的患者中发现:1例报告

Ibrain Pub Date : 2023-08-14 DOI: 10.1002/ibra.12125

Jiahui Qin, Zun‐Lin Zhou, Yuankun Zhou, Ya Chen, Xiao‐Yan Yang, Hai‐Qing Zhang, Zucai Xu

{"title":"Double gene mutations of LRSAM1 and REEP1 and a new REEP1 mutation site found in a patient with amyotrophic lateral sclerosis with subjective paresthesia: A case report","authors":"Jiahui Qin, Zun‐Lin Zhou, Yuankun Zhou, Ya Chen, Xiao‐Yan Yang, Hai‐Qing Zhang, Zucai Xu","doi":"10.1002/ibra.12125","DOIUrl":"https://doi.org/10.1002/ibra.12125","url":null,"abstract":"Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective degeneration of upper and lower motor neurons. Although dyskinesia is the most prominent clinical manifestation of ALS, with an in‐depth understanding of disease pathogenesis and clinical detection, more and more ALS patients are found to have nonmotor symptoms, such as sensory impairment. Genetic testing technology has developed rapidly in recent years. New genes have been proven to be involved in the pathogenesis of ALS. However, according to the existing research evidence, no literature has reported that patients with ALS have leucine‐rich repeats and sterility α mutations in motif 1 (LRSAM1) and receptor expression accessory protein 1 (REEP1). The mutation sites of REEP1 gene have not been reported, and the simultaneous mutations of two genes have not been reported. In the largest human gene mutation frequency database gnomad, the mutation sites of two genes are currently defined as new heterozygous variants with unclear clinical significance. Therefore, this article reports the clinical data of this case to further deepen the clinicians' understanding of the disease, and may provide evidence for further study of the new genotype–phenotype of LRSAM1 and REEP1.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86594547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold and silver nanoparticles in Alzheimer's and Parkinson's diagnostics and treatments 阿尔茨海默氏症和帕金森氏症诊断和治疗中的金和银纳米颗粒。

Ibrain Pub Date : 2023-08-14 DOI: 10.1002/ibra.12126

Edoardo Scarpa, Mariafrancesca Cascione, Anna Griego, Paolo Pellegrino, Giorgia Moschetti, Valeria De Matteis

{"title":"Gold and silver nanoparticles in Alzheimer's and Parkinson's diagnostics and treatments","authors":"Edoardo Scarpa, Mariafrancesca Cascione, Anna Griego, Paolo Pellegrino, Giorgia Moschetti, Valeria De Matteis","doi":"10.1002/ibra.12126","DOIUrl":"10.1002/ibra.12126","url":null,"abstract":"<p>Neurodegenerative diseases (NDs) impose substantial medical and public health burdens on people worldwide and represent one of the major threats to human health. The prevalence of these age-dependent disorders is dramatically increasing over time, a process intrinsically related to a constantly rising percentage of the elderly population in recent years. Among all the NDs, Alzheimer's and Parkinson's are considered the most debilitating as they cause memory and cognitive loss, as well as severely affecting basic physiological conditions such as the ability to move, speak, and breathe. There is an extreme need for new and more effective therapies to counteract these devastating diseases, as the available treatments are only able to slow down the pathogenic process without really stopping or resolving it. This review aims to elucidate the current nanotechnology-based tools representing a future hope for NDs treatment. Noble metal nano-systems, that is, gold and silver nanoparticles (NPs), have indeed unique physicochemical characteristics enabling them to deliver any pharmacological treatment in a more effective way within the central nervous system. This can potentially make NPs a new hope for reversing the actual therapeutic strategy based on slowing down an irreversible process into a more effective and permanent treatment.</p>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41180784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA methylation: The epigenetic mechanism of Alzheimer's disease DNA 甲基化：阿尔茨海默病的表观遗传学机制

Ibrain Pub Date : 2023-08-10 DOI: 10.1002/ibra.12121

Hao-Yue Qin, Jiao-Yan Liu, Chang-Le Fang, Yan-Ping Deng, Ying Zhang

引用次数: 0

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