绿色银纳米粒子:针对神经退行性细胞系模型的前瞻性纳米工具

Ibrain Pub Date : 2024-05-23 DOI:10.1002/ibra.12157
Valeria De Matteis, Simona Martano, Paolo Pellegrino, Chiara Ingrosso, Daniele Costa, Stefano Mazzotta, Jose L. Toca-Herrera, Rosaria Rinaldi, Mariafrancesca Cascione
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引用次数: 0

摘要

神经退行性疾病是近十年来日益严峻的挑战,其主要原因是全球人口老龄化。目前的工作重点是实施各种策略来减轻神经退行性疾病的不利影响,目的是减缓病理进展。值得注意的是,近年来,人们发现使用纳米粒子(NPs),尤其是通过绿色化学工艺获得的纳米粒子,可以成为一种前景广阔的治疗方法。与通过传统化学途径合成的纳米粒子相比,完全来自植物化学物质的绿色纳米粒子被认为更安全。在本研究中,我们评估了源于绿色化学的银纳米粒子(AgNPs)在神经母细胞瘤细胞 SHSY-5Y 中的作用,SHSY-5Y 被认为是研究神经退行性疾病的重要模型。具体来说,我们使用了两种不同浓度(0.5 和 1 µM)的 AgNPs 和两个时间点(24 和 48 小时)来评估其对神经母细胞瘤细胞的影响,观察其存活率的降低和细胞内钙的产生,尤其是在 48 小时内使用 1 µM。共聚焦显微镜采集以及肌动蛋白纤维的一致性和密度分析进一步证实了这一点。我们的体外研究结果表明,绿色银纳米粒子对神经退行性病变具有潜在疗效;因此,必须进一步开展体内研究,以优化可能的治疗方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Green silver nanoparticles: Prospective nanotools against neurodegenerative cell line model

Green silver nanoparticles: Prospective nanotools against neurodegenerative cell line model

Neurodegenerative diseases represent an increasingly burdensome challenge of the past decade, primarily driven by the global aging of the population. Ongoing efforts focus on implementing diverse strategies to mitigate the adverse effects of neurodegeneration, with the goal of decelerating the pathology progression. Notably, in recent years, it has emerged that the use of nanoparticles (NPs), particularly those obtained through green chemical processes, could constitute a promising therapeutic approach. Green NPs, exclusively sourced from phytochemicals, are deemed safer compared to NPs synthetized through conventional chemical route. In this study, the effects of green chemistry-derived silver NPs (AgNPs) were assessed in neuroblastoma cells, SHSY-5Y, which are considered a pivotal model for investigating neurodegenerative diseases. Specifically, we used two different concentrations (0.5 and 1 µM) of AgNPs and two time points (24 and 48 h) to evaluate the impact on neuroblastoma cells by observing viability reduction and intracellular calcium production, especially using 1 µM at 48 h. Furthermore, investigation using atomic force microscopy (AFM) unveiled an alteration in Young's modulus due to the reorganization of cortical actin following exposure to green AgNPs. This evidence was further corroborated by confocal microscopy acquisitions as well as coherency and density analyses on actin fibers. Our in vitro findings suggest the potential efficacy of green AgNPs against neurodegeneration; therefore, further in vivo studies are imperative to optimize possible therapeutic protocols.

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