Ibrain Pub Date : 2022-08-19 DOI: 10.1002/ibra.12060

Xue-Yan Huang, Jun-Yan Zhang, Chang-Yin Yu

{"title":"Whether it is safe to start anticoagulation after intracranial hemorrhage within 2 weeks: A systematic review and meta-analysis","authors":"Xue-Yan Huang, Jun-Yan Zhang, Chang-Yin Yu","doi":"10.1002/ibra.12060","DOIUrl":"10.1002/ibra.12060","url":null,"abstract":"Whether restarting anticoagulation (RA) treatment after intracranial hemorrhage (ICH) is still controversial. We performed a systematic review and meta-analysis to summarize the relationship between anticoagulation after ICH with the recurrence of hemorrhagic events, ischemic events, and long-term mortality. Medline, Embase, and the Cochrane Central Register of Controlled Trials, from inception to November 2020. We searched the published medical literature to ensure cohort studies involving ICH associated with anticoagulation in adults. Primary outcomes were long-term mortality, hemorrhagic events, and ischemic events (myocardial infarction, pulmonary embolism, ischemic stroke, or systemic embolization). We concluded seven retrospective cohorts, including 1876 intracranial hemorrhage patients with indications of anticoagulation. The ratio of the anticoagulant restart was 35.3% (664n). RA was associated with a significantly lower incidence of recurrent ischemic events (pooled odds ratio [OR] 0.29, 95% confidence interval [CI] 0.19% to 0.45%, p = 0.97) and death events (pooled OR 0.56, 95% CI 0.40%–0.79%, p = 0.27). There is no evidence that early recovery of anticoagulation (within 2 weeks or 1 month) is associated with the occurrence of hemorrhagic events (within 2 weeks: pooled OR 0.80, 95% CI 0.3–2.12, p = 0.52 vs. within 1 month: pooled OR 1.14, 95% CI 0.77–1.68, p = 0.82). Based on these, recovery of anticoagulation after ICH is beneficial for long-term mortality and recurrence of ischemic events. The meta-analysis showed a resumption of oral anticoagulation within 2 weeks or 1 month in patients who had a cerebral hemorrhage was beneficial and did not increase the risk of hemorrhagic events and reduced the occurrence of ischemic and fatal endpoint events.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 3","pages":"377-388"},"PeriodicalIF":0.0,"publicationDate":"2022-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41126108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AQP4 knockout promotes neurite outgrowth via upregulating GAP43 expression in infant rats with hypoxic-ischemic brain injury 在缺氧缺血性脑损伤幼鼠中，敲除AQP4通过上调GAP43表达促进神经突生长

Ibrain Pub Date : 2022-08-19 DOI: 10.1002/ibra.12062

Qi-Qin Dan, Zheng Ma, Ya-Xin Tan, Belegu Visar, Li Chen

{"title":"AQP4 knockout promotes neurite outgrowth via upregulating GAP43 expression in infant rats with hypoxic-ischemic brain injury","authors":"Qi-Qin Dan, Zheng Ma, Ya-Xin Tan, Belegu Visar, Li Chen","doi":"10.1002/ibra.12062","DOIUrl":"10.1002/ibra.12062","url":null,"abstract":"Neonatal hypoxic-ischemic encephalopathy (NHIE) induces severe cerebral damage and neurological dysfunction, with seldom effective therapy. Aquaporin-4 (AQP4) is involved in aggravating brain damage induced by NHIE. This study aimed to investigate the role of AQP4 underlying the pathogenesis of NHIE. Neonatal Sprague–Dawley rats were used to establish neonatal hypoxic-ischemic (HI) models, and the expression of AQP4 in the cortex, hippocampus, and lung tissues was detected by real-time quantitative polymerase chain reaction as well as Western blot. Primary cortical neurons were cultured for the oxygen-glucose deprivation (OGD) model, and siRNA was used to silence the expression of AQP4. Immunostaining of Tuj1 was performed to observe the axonal growth. CRISPER/Cas9 technology was used to knock out AQP4. The results demonstrated that AQP4 was upregulated in the cortex, hippocampus, and lung tissues in neonatal rats with HI and OGD neurons. Besides, silencing AQP4 promoted axonal growth of OGD neurons, and AQP4 knockout notably improved long-term neurobehavioral impairment. Furthermore, GAP43 was found closely correlated with AQP4 via GeneMANIA prediction. Significant downregulation of GAP43 was induced in OGD neurons, while AQP4 knockout markedly upregulated its expression in rats. This indicated that the depletion of AQP4 may enhance axonal regeneration and promote the long-term neurobehavioral recovery associated with the upregulation of GAP43 expression.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 3","pages":"324-337"},"PeriodicalIF":0.0,"publicationDate":"2022-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41143203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of the ultrasound-guided nerve block technique for nonanalgesic effects 超声引导神经阻滞技术在非镇痛效果中的应用

Ibrain Pub Date : 2022-08-15 DOI: 10.1002/ibra.12061

Guang-Ting Zhang, Feng-Lin Wang, Ying Ran, De-Xing Liu

引用次数: 1

Brain structure analysis of different age groups of Diannan small-ear pigs 滇南小耳猪不同年龄组脑结构分析

Ibrain Pub Date : 2022-08-13 DOI: 10.1002/ibra.12058

Yi-Fan Liu, Chang-Le Fang, Yu Pi, Teng-Fei Ke, Ji-Xiang Chu, Lin-Na Tang, Lan-Chun Zhang, Somjit Wanchana, Cheng-De Liao

{"title":"Brain structure analysis of different age groups of Diannan small-ear pigs","authors":"Yi-Fan Liu, Chang-Le Fang, Yu Pi, Teng-Fei Ke, Ji-Xiang Chu, Lin-Na Tang, Lan-Chun Zhang, Somjit Wanchana, Cheng-De Liao","doi":"10.1002/ibra.12058","DOIUrl":"10.1002/ibra.12058","url":null,"abstract":"The objective of the study is to investigate the brain development and atrophy of Diannan small-ear pigs in different ages using magnetic resonance imaging (MRI). A total of 12 Diannan small-ear pigs were included and divided into the young group, adult group, and middle-and-old age (M&O) group according to their age. The brain structure of pigs was scanned using MRI, and the brain data obtained were statistically analyzed by signal conversion and image reconstruction. Compared with the young group, the signals of most brain structures in the adult group and M&O group were significantly decreased (p < 0.05). Compared with the adult group, the signal intensity of the right caudate nucleus and the right lateral ventricle in the M&O group was significantly increased, while the signal intensity of other regions was almost significantly decreased (p < 0.05). Compared with the young group, both adult and M&O groups had some degree of brain atrophy. Brain atrophy in the precuneus and the inferior temporal gyrus was more predominant in the M&O group in comparison with the adult group. The present study demonstrated that the brain signal of Diannan small-ear pigs gradually diminished with age, while the degree of brain atrophy was the opposite, providing the basic data on the brain of Diannan small-ear pigs.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 3","pages":"314-323"},"PeriodicalIF":0.0,"publicationDate":"2022-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41165749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The alternative 3′ splice site of GPNMB may promote neuronal survival after neonatal hypoxic–ischemic encephalopathy injury GPNMB的替代3 '剪接位点可能促进新生儿缺氧缺血性脑病损伤后神经元的存活

Ibrain Pub Date : 2022-08-09 DOI: 10.1002/ibra.12056

Guo-Jiao Chen, Shan-Shan Yan, Jing-Han Zhang, Ji Zhang, Isaac Bul Deng, Rong He

{"title":"The alternative 3′ splice site of GPNMB may promote neuronal survival after neonatal hypoxic–ischemic encephalopathy injury","authors":"Guo-Jiao Chen, Shan-Shan Yan, Jing-Han Zhang, Ji Zhang, Isaac Bul Deng, Rong He","doi":"10.1002/ibra.12056","DOIUrl":"10.1002/ibra.12056","url":null,"abstract":"This study aimed to decipher the effect of glycoprotein nonmetastatic melanoma protein B (GPNMB) on neonatal hypoxic–ischemic encephalopathy (NHIE) and its potential molecular mechanism. The hypoxic–ischemic (HI) model was established in 7-day-old rats, and then, Zea-Longa scores and Nissl staining were performed to measure brain damage post-HI. In addition, gene sequencing was used to detect the differential expression genes (DEGs), and then, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases were used to determine the function of DEGs. Furthermore, an oxygen–glucose deprivation (OGD) model was developed in SY5Y cells and human fetal neurons, and then, the level of GPNMB was verified by quantitative real-time polymerase chain reaction. In addition, methyl thiazolyl tetrazolium and cell counting kit-8 assays were applied after GPNMB interference. Finally, the alternative splicing of GPNMB expression was analyzed using Splice Grapher software. The results indicated that HI induced marked neurological impairment and neuron injury in rats. Also, GPNMB was the most obviously upregulated gene in DEGs. Additionally, GPNMB was upregulated significantly in SY5Y and fetal neurons after OGD, and GPNMB-si promoted an increase in cell viability and number. Moreover, we found that the GPNMB alternative splicing type was the Alternative 3′ splice site, with the alternative splicing site in 143382985:143404102. Herein, GPNMB promotes a crucial regulatory mechanism with alternative splicing for neuronal survival after NHIE.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 3","pages":"302-313"},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10529014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The synergistic ameliorative activity of peroxisome proliferator-activated receptor-alpha and gamma agonists, fenofibrate and pioglitazone, on hippocampal neurodegeneration in a rat model of insulin resistance 过氧化物酶体增殖体激活受体- α和γ激动剂非诺贝特和吡格列酮对胰岛素抵抗大鼠模型海马神经变性的协同改善活性

Ibrain Pub Date : 2022-08-08 DOI: 10.1002/ibra.12059

Olumayowa K. Idowu, Olushola O. Oluyomi, Oluwatomisin O. Faniyan, Olufunke O. Dosumu, Oluwole B. Akinola

{"title":"The synergistic ameliorative activity of peroxisome proliferator-activated receptor-alpha and gamma agonists, fenofibrate and pioglitazone, on hippocampal neurodegeneration in a rat model of insulin resistance","authors":"Olumayowa K. Idowu, Olushola O. Oluyomi, Oluwatomisin O. Faniyan, Olufunke O. Dosumu, Oluwole B. Akinola","doi":"10.1002/ibra.12059","DOIUrl":"10.1002/ibra.12059","url":null,"abstract":"Insulin resistance (IR) is a risk factor for metabolic disorders and neurodegeneration. Peroxisome proliferator-activated receptor (PPAR) agonists have been proven to mitigate the neuronal pathology associated with IR. However, the synergetic efficacy of these agonists is yet to be fully described. Hence, we aimed to investigate the efficacy of PPARα/γ agonists (fenofibrate and pioglitazone) on a high-fat diet (HFD) and streptozotocin (STZ)-induced hippocampal neurodegeneration. Male Wistar rats (200 ± 25 mg/body weight [BW]) were divided into five groups. The experimental groups were fed on an HFD for 12 weeks coupled with 5 days of an STZ injection (30 mg/kg/BW, i.p) to induce IR. Fenofibrate (FEN; 100 mg/kg/BW, orally), pioglitazone (PIO; 20 mg/kg/BW, orally), and their combination were administered for 2 weeks postinduction. Behavioral tests were conducted, and blood was collected to determine insulin sensitivity after treatment. Animals were killed for assessment of oxidative stress, cellular morphology characterization, and astrocytic evaluation. HFD/STZ-induced IR increased malondialdehyde (MDA) levels and decreased glutathione (GSH) levels. Evidence of cellular alterations and overexpression of astrocytic protein was observed in the hippocampus. By contrast, monotherapy of FEN and PIO increased the GSH level (p < 0.05), decreased the MDA level (p < 0.05), and improved cellular morphology and astrocytic expression. Furthermore, the combined treatment led to improved therapeutic activities compared to monotherapies. In conclusion, FEN and PIO exerted a therapeutic synergistic effect on HFD/STZ-induced IR in the hippocampus.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 3","pages":"251-263"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41170277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Invisible doppelgänger and body image disorders in right superior parietal lobule stroke, a case series 右脑顶叶上叶中风的无形doppelgänger与身体形象障碍:一个病例系列

Ibrain Pub Date : 2022-08-08 DOI: 10.1002/ibra.12057

Emre Kumral, Fatma E. Çetin, Birgül Dere, Hüseyin N. Özdemir

{"title":"Invisible doppelgänger and body image disorders in right superior parietal lobule stroke, a case series","authors":"Emre Kumral, Fatma E. Çetin, Birgül Dere, Hüseyin N. Özdemir","doi":"10.1002/ibra.12057","DOIUrl":"10.1002/ibra.12057","url":null,"abstract":"Autoscopic phenomena or an “invisible doppelgänger” refer to the illusory reduplication of one's own body. Body image disorder involves distorted perception or decreased body awareness. In the literature, feeling of presence (FOP) is rarely presented with a circumscribed cerebral pathology due to acute vascular lesions, and concomitant FOP and disorders of the body image or the body schema (BIBS) have rarely been reported. We present three cases of both FOP and BIBS disorders. All patients reported the two types of symptoms almost simultaneously: The first patient had the symptom of somatoparaphrenia characterized by deny ownership of the hand and feeling that it does not belong to her, the second patient had the sign of fading limb presented with misuse of his left hand when it was not under visual control and could not mentally represent and locate this part of the body in space, and the third patient had autotopagnosia; he was unable to localize any touched area below the elbow and knee. All patients had right parietal ischemic lesions involving the superior parietal lobule, and two patients had an adjacent additional precuneal involvement. Based on the cases presented here, it is plausible that BIBS may develop in addition to FOP, especially in lesions involving the superior parietal lobule and precuneus.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 3","pages":"401-405"},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41160624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF 电针通过CD4和BDNF的协同作用促进脊髓挫伤大鼠神经再生和功能恢复

Ibrain Pub Date : 2022-07-26 DOI: 10.1002/ibra.12055

Bao-Lei Zhang, Xi-Liang Guo

{"title":"Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF","authors":"Bao-Lei Zhang, Xi-Liang Guo","doi":"10.1002/ibra.12055","DOIUrl":"10.1002/ibra.12055","url":null,"abstract":"To explore the effect of electroacupuncture on spinal cord injury (SCI) involving immune-related factors and regeneration-related factors in rats. The model of spinal cord contusion was established by PCI 3000 instrument. Two types of acupuncture points were selected for electroacupuncture treatment on rats. The rats were tested once a week, and the fiber remodeling was detected by magnetic resonance imaging. Transcriptome sequencing was performed on spinal scar samples. Using Python to write code, statistical analysis and bioinformatics analysis of the correlation between transcriptome sequencing data and fiber reconstruction results are carried out. Lastly, the expression of CD4 and brain-derived neurotrophic factor (BDNF) in spinal cord scar was verified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Electroacupuncture exhibited a positive effect on the recovery of motor function in rats after SCI. Bioinformatics analysis found a direct interaction between CD4 and BDNF. Transcriptome sequencing and PCR results verified that electroacupuncture significantly reduced the expression of CD4, and increased significantly the expression of BDNF, simultaneously corresponding to nerve regeneration in rats with SCI. Our results showed that electroacupuncture intervention in SCI rats improves neural behavior via inhibiting the expression of CD4 and increasing the expression of BDNF.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 3","pages":"285-301"},"PeriodicalIF":0.0,"publicationDate":"2022-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10529162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulating EVA1C exerts the potential to promote neuron growth after neonatal hypoxic-ischemic encephalopathy injury associated with alternative splicing 下调EVA1C有可能促进新生儿缺氧缺血性脑病损伤后神经元的生长，并伴有选择性剪接

Ibrain Pub Date : 2022-07-21 DOI: 10.1002/ibra.12053

Yue Hu, Rong Rong, Yi Wang, Shan-Shan Yan, Su Liu, Lei Wang

{"title":"Downregulating EVA1C exerts the potential to promote neuron growth after neonatal hypoxic-ischemic encephalopathy injury associated with alternative splicing","authors":"Yue Hu, Rong Rong, Yi Wang, Shan-Shan Yan, Su Liu, Lei Wang","doi":"10.1002/ibra.12053","DOIUrl":"10.1002/ibra.12053","url":null,"abstract":"Neonatal hypoxic-ischemic encephalopathy (NHIE) is one of the major diseases in newborns during the perinatal stage, which globally is the main reason for children's morbidity and mortality. However, the mechanism of NHIE still remains poorly clear. In this study, the 7-day-old rats were subjected to hypoxic-ischemia (HI), then brain damage was detected. Afterward, the expression of eva-1 homolog C (EVA1C) was measured in vitro by establishing the oxygen-glucose deprivation (OGD) model in SHSY5Y cells and human fetal neurons. Subsequently, the potential function and mechanism of EVA1C were explored by silencing EVA1C and alternative splicing prediction. As a result, obvious neurobehavioral impairment and brain infarction were detected through Zea-Longa score and TTC staining; meanwhile, neuron injury was tested by HE and Nissl staining post HI. Moreover, it was found that the expression of EVA1C was notably upregulated in SHSY5Y cells and human fetal neurons after OGD. In addition, cell survival and growth were increased after silencing EVA1C, which might be associated with alternative splicing. In conclusion, EVA1C interference exhibited potential in promoting neuron survival and growth, associated with exon skipping with the alternative splicing site in 34613318:34687258, which may provide the basis for the therapeutic target and mechanism research of NHIE.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 4","pages":"481-491"},"PeriodicalIF":0.0,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10529346/pdf/IBRA-8-481.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41165804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treadmill training improves cognitive function by increasing IGF2 targeted downregulation of miRNA-483 跑步机训练通过增加IGF2靶向miRNA-483的下调来改善认知功能

Ibrain Pub Date : 2022-07-21 DOI: 10.1002/ibra.12051

Xiu-Juan Dong, Jun-Jie Chen, Lu-Lu Xue, Mohammed Al-hawwas

{"title":"Treadmill training improves cognitive function by increasing IGF2 targeted downregulation of miRNA-483","authors":"Xiu-Juan Dong, Jun-Jie Chen, Lu-Lu Xue, Mohammed Al-hawwas","doi":"10.1002/ibra.12051","DOIUrl":"10.1002/ibra.12051","url":null,"abstract":"Optimal exercise can promote the development of cognitive functions. Nevertheless, mechanisms that elicit these positive effects of exercise still need to be elucidated. Insulin-like growth factor 2 (IGF2) is known to act as a potent enhancer of memory and cognitive functions, whereas the mechanism by which IGF2 regulates cognitive functions in terms of moderate treadmill exercise remains largely vague. In the study, rats were subjected to low-, moderate-, and high-intensity treadmill training for 6 weeks. Then, the Morris water maze test was used to investigate spatial learning and memory ability in rats subjected to treadmill exercises of different intensities. Subsequently, gene chip and bioinformatics analyses were used to explore IGF2 and predict target microRNAs (miRNAs). Quantitative real-time polymerase chain reaction, western blot, and immunofluorescence analysis were performed to detect the levels of IGF2. Furthermore, IGF2-small interfering RNA, the miRNA-483-mimic, and the miRNA-483-inhibitor were transfected to determine the role of IGF2 and miRNA-483 in the growth of hippocampal neurons. The results of the Morris water maze test showed that moderate-intensity treadmill training enhanced cognitive functions; meanwhile, the expression of IGF2 was significantly upregulated in the hippocampus after moderate-intensity treadmill exercise. From databases, miRNA-483 was screened and predicted as the target gene of IGF2. Moreover, silencing IGF2 inhibited neurite growth in the hippocampus of rats, the miRNA-483-inhibitor ameliorated silencing IGF2 induced impairment of hippocampal neurons. These findings suggested that treadmill training could enhance cognitive functions, wherein the underlying mechanism involved an increase in the expression of IGF2 and downregulation of miRNA-483.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"8 3","pages":"264-275"},"PeriodicalIF":0.0,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10529000/pdf/IBRA-8-264.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41176414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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