基于网络药理学和实验验证的三七皂苷抗衰老的系统机制

Ibrain Pub Date : 2024-06-01 DOI:10.1002/ibra.12165
Yang‐Yang Zhao, Li‐Xia Yang, Shuang‐Yu Que, Lei‐Xing An, Abeer A. Teeti, Shun‐Wu Xiao
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引用次数: 0

摘要

本研究旨在利用网络药理学结合实验验证的方法研究三七皂苷(PNS)抗衰老的系统机制。研究使用String数据库和Cytoscape3.7.2进行蛋白相互作用(PPI),构建基因网络。利用基因本体(GO)和京都基因组百科全书(KEGG)分析了关键靶基因。然后,通过反转录聚合酶链反应在 SAM-P/8 小鼠中验证了衰老相关基因,并与 PNS 的主要成分进行了分子对接。此外,它还产生了枢纽基因和差异基因之间的聚类。GO和KEGG的结果表明,PNS的抗衰老作用是由细胞凋亡、癌症和神经变性介导的,8个Hub基因中有5个与PNS的主要成分具有良好的结合活性。此外,动物实验报告显示,MAP2、MAPKK4、RAB6A 和 Sortilin-1 在衰老小鼠的脑组织中有不同程度的表达,并与 PNS 的主要活性成分有良好的对接结合。然而,169个PNS交叉基因与4个差异基因之间没有交叉,而它们从PPI中产生了联系,其中MAP2K4只与AKT1和CASP3有联系;MAP2只与AKT1和CASP3有联系;RAB6A只与AKT1有联系;但Sortilin-1与枢纽基因没有联系。总之,PNS 的抗衰老作用与 8 个 Hub 基因和 4 个差异基因有关。所有这些基因都组成了一个可能与 PNS 的抗衰老效应有关的基因簇或基因群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Systemic mechanism of Panax noteginseng saponins in antiaging based on network pharmacology combined with experimental validation
This study aims to investigate the systemic mechanism of Panax notoginseng saponins (PNS) in antiaging using network pharmacology combined with experimental validation. String database and Cytoscape3.7.2 were used to perform the protein–protein interaction (PPI) and construct genes network. The key target genes were analyzed using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Then, the aging‐related genes were verified by reverse‐transcription polymerase chain reaction in SAM‐P/8 mice, and performed molecular docking with the main components of PNS. Moreover, it produced cluster between Hub genes and differential genes. A total of 169 crossover genes were obtained, and the results of GO and KEGG indicated that the antiaging effect of PNS was mediated by apoptosis, cancer, and neurodegeneration and that five of the eight Hub genes had good binding activity with the main components of PNS. In addition, animal experiments reported that MAP2, MAPKK4, RAB6A, and Sortilin‐1 have different levels of expression in the brain tissues of aging mice, and bind well docking with the main active components of PNS. However, there was no crossover between the 169 PNS intersecting genes and the four differential genes, while they yielded a link from PPI in which MAP2K4 was only linked to AKT1 and CASP3; MAP2 was only linked to AKT1 and CASP3; RAB6A was only linked to AKT1; but Sortlin‐1 did not link to the Hub genes. In summary, the antiaging effect of PNS is associated with the eight Hub genes and four differential genes. All of them consist of a cluster or group that is possibly related to the antiaging effect of PNS.
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