Cell host & microbe最新文献_第4页

Unbiased discovery of natural sequence variants that influence fungal virulence. 影响真菌毒力的自然序列变异的无偏见发现。

Cell host & microbe Pub Date : 2023-11-08 Epub Date: 2023-10-27 DOI: 10.1016/j.chom.2023.10.002

Daniel Paiva Agustinho, Holly Leanne Brown, Guohua Chen, Elizabeth Anne Gaylord, Jennifer Geddes-McAlister, Michael Richard Brent, Tamara Lea Doering

{"title":"Unbiased discovery of natural sequence variants that influence fungal virulence.","authors":"Daniel Paiva Agustinho, Holly Leanne Brown, Guohua Chen, Elizabeth Anne Gaylord, Jennifer Geddes-McAlister, Michael Richard Brent, Tamara Lea Doering","doi":"10.1016/j.chom.2023.10.002","DOIUrl":"10.1016/j.chom.2023.10.002","url":null,"abstract":"Isolates of Cryptococcus neoformans, a fungal pathogen that kills over 112,000 people each year, differ from a 19-Mb reference genome at a few thousand up to almost a million DNA sequence positions. We used bulked segregant analysis and association analysis, genetic methods that require no prior knowledge of sequence function, to address the key question of which naturally occurring sequence variants influence fungal virulence. We identified a region containing such variants, prioritized them, and engineered strains to test our findings in a mouse model of infection. At one locus, we identified a 4-nt variant in the PDE2 gene that occurs in common laboratory strains and severely truncates the encoded phosphodiesterase. The resulting loss of phosphodiesterase activity significantly impacts virulence. Our studies demonstrate a powerful and unbiased strategy for identifying key genomic regions in the absence of prior information and provide significant sequence and strain resources to the community.","PeriodicalId":93926,"journal":{"name":"Cell host & microbe","volume":" ","pages":"1910-1920.e5"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10842055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66784768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the post-vaccination era: Do emerging HPV types represent a new threat? 利用后疫苗接种时代：新出现的HPV类型是否代表了一种新的威胁？

Cell host & microbe Pub Date : 2023-11-08 DOI: 10.1016/j.chom.2023.10.013

Sergio Ponce-de-Leon, Marcela Lizano

引用次数: 0

Trained immunity: Target for prophylaxis and therapy. 经过训练的免疫力：预防和治疗的目标。

Cell host & microbe Pub Date : 2023-11-08 DOI: 10.1016/j.chom.2023.10.015

Athanasios Ziogas, Mariolina Bruno, Roy van der Meel, Willem J M Mulder, Mihai G Netea

引用次数: 1

Putting the pieces together: Chimeric virus strategy decode Dengue virus 3 antibody responses. 拼凑：嵌合病毒策略解码登革热病毒3抗体反应。

Cell host & microbe Pub Date : 2023-11-08 DOI: 10.1016/j.chom.2023.10.014

Naoko Uno, Ted M Ross

引用次数: 0

The plant immune receptor SNC1 monitors helper NLRs targeted by a bacterial effector. 植物免疫受体SNC1监测细菌效应物靶向的辅助性NLR。

Cell host & microbe Pub Date : 2023-11-08 DOI: 10.1016/j.chom.2023.10.006

Ming-Yu Wang, Jun-Bin Chen, Rui Wu, Hai-Long Guo, Yan Chen, Zhen-Ju Li, Lu-Yang Wei, Chuang Liu, Sheng-Feng He, Mei-Da Du, Ya-Long Guo, You-Liang Peng, Jonathan D G Jones, Detlef Weigel, Jian-Hua Huang, Wang-Sheng Zhu

{"title":"The plant immune receptor SNC1 monitors helper NLRs targeted by a bacterial effector.","authors":"Ming-Yu Wang, Jun-Bin Chen, Rui Wu, Hai-Long Guo, Yan Chen, Zhen-Ju Li, Lu-Yang Wei, Chuang Liu, Sheng-Feng He, Mei-Da Du, Ya-Long Guo, You-Liang Peng, Jonathan D G Jones, Detlef Weigel, Jian-Hua Huang, Wang-Sheng Zhu","doi":"10.1016/j.chom.2023.10.006","DOIUrl":"10.1016/j.chom.2023.10.006","url":null,"abstract":"Plants deploy intracellular receptors to counteract pathogen effectors that suppress cell-surface-receptor-mediated immunity. To what extent pathogens manipulate intracellular receptor-mediated immunity, and how plants tackle such manipulation, remains unknown. Arabidopsis thaliana encodes three similar ADR1 class helper nucleotide-binding domain leucine-rich repeat receptors (ADR1, ADR1-L1, and ADR1-L2), which are crucial in plant immunity initiated by intracellular receptors. Here, we report that Pseudomonas syringae effector AvrPtoB suppresses ADR1-L1- and ADR1-L2-mediated cell death. ADR1, however, evades such suppression by diversifying into two ubiquitination sites targeted by AvrPtoB. The intracellular sensor SNC1 interacts with and guards the CCR domains of ADR1-L1/L2. Removal of ADR1-L1/L2 or delivery of AvrPtoB activates SNC1, which then signals through ADR1 to trigger immunity. Our work elucidates the long-sought-after function of SNC1 in defense, and also how plants can use dual strategies, sequence diversification, and a multi-layered guard-guardee system, to counteract pathogen's attack on core immunity functions.","PeriodicalId":93926,"journal":{"name":"Cell host & microbe","volume":"31 11","pages":"1792-1803.e7"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72016369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A gB nanoparticle vaccine elicits a protective neutralizing antibody response against EBV. gB纳米粒子疫苗引发针对EBV的保护性中和抗体反应。

Cell host & microbe Pub Date : 2023-11-08 Epub Date: 2023-10-16 DOI: 10.1016/j.chom.2023.09.011

Cong Sun, Yin-Feng Kang, Xin-Yan Fang, Yi-Na Liu, Guo-Long Bu, Ao-Jie Wang, Yan Li, Qian-Ying Zhu, Hua Zhang, Chu Xie, Xiang-Wei Kong, Yong-Jian Peng, Wen-Jie Lin, Ling Zhou, Xin-Chun Chen, Zheng-Zhou Lu, Hui-Qin Xu, Dong-Chun Hong, Xiao Zhang, Ling Zhong, Guo-Kai Feng, Yi-Xin Zeng, Miao Xu, Qian Zhong, Zheng Liu, Mu-Sheng Zeng

{"title":"A gB nanoparticle vaccine elicits a protective neutralizing antibody response against EBV.","authors":"Cong Sun, Yin-Feng Kang, Xin-Yan Fang, Yi-Na Liu, Guo-Long Bu, Ao-Jie Wang, Yan Li, Qian-Ying Zhu, Hua Zhang, Chu Xie, Xiang-Wei Kong, Yong-Jian Peng, Wen-Jie Lin, Ling Zhou, Xin-Chun Chen, Zheng-Zhou Lu, Hui-Qin Xu, Dong-Chun Hong, Xiao Zhang, Ling Zhong, Guo-Kai Feng, Yi-Xin Zeng, Miao Xu, Qian Zhong, Zheng Liu, Mu-Sheng Zeng","doi":"10.1016/j.chom.2023.09.011","DOIUrl":"10.1016/j.chom.2023.09.011","url":null,"abstract":"Epstein-Barr virus (EBV) is a global public health concern, as it is known to cause multiple diseases while also being etiologically associated with a wide range of epithelial and lymphoid malignancies. Currently, there is no available prophylactic vaccine against EBV. gB is the EBV fusion protein that mediates viral membrane fusion and participates in host recognition, making it critical for EBV infection in both B cells and epithelial cells. Here, we present a gB nanoparticle, gB-I53-50 NP, that displays multiple copies of gB. Compared with the gB trimer, gB-I53-50 NP shows improved structural integrity and stability, as well as enhanced immunogenicity in mice and non-human primate (NHP) preclinical models. Immunization and passive transfer demonstrate a robust and durable protective antibody response that protects humanized mice against lethal EBV challenge. This vaccine candidate demonstrates significant potential in preventing EBV infection, providing a possible platform for developing prophylactic vaccines for EBV.","PeriodicalId":93926,"journal":{"name":"Cell host & microbe","volume":" ","pages":"1882-1897.e10"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium tuberculosis suppresses host DNA repair to boost its intracellular survival. 结核分枝杆菌抑制宿主DNA修复以提高其细胞内存活率。

Cell host & microbe Pub Date : 2023-11-08 Epub Date: 2023-10-16 DOI: 10.1016/j.chom.2023.09.010

Shanshan Liu, Liru Guan, Cheng Peng, Yuanna Cheng, Hongyu Cheng, Fei Wang, Mingtong Ma, Ruijuan Zheng, Zhe Ji, Pengfei Cui, Yefei Ren, Liru Li, Chenyue Shi, Jie Wang, Xiaochen Huang, Xia Cai, Di Qu, Haiping Zhang, Zhiyong Mao, Haipeng Liu, Peng Wang, Wei Sha, Hua Yang, Lin Wang, Baoxue Ge

{"title":"Mycobacterium tuberculosis suppresses host DNA repair to boost its intracellular survival.","authors":"Shanshan Liu, Liru Guan, Cheng Peng, Yuanna Cheng, Hongyu Cheng, Fei Wang, Mingtong Ma, Ruijuan Zheng, Zhe Ji, Pengfei Cui, Yefei Ren, Liru Li, Chenyue Shi, Jie Wang, Xiaochen Huang, Xia Cai, Di Qu, Haiping Zhang, Zhiyong Mao, Haipeng Liu, Peng Wang, Wei Sha, Hua Yang, Lin Wang, Baoxue Ge","doi":"10.1016/j.chom.2023.09.010","DOIUrl":"10.1016/j.chom.2023.09.010","url":null,"abstract":"Mycobacterium tuberculosis (Mtb) triggers distinct changes in macrophages, resulting in the formation of lipid droplets that serve as a nutrient source. We discover that Mtb promotes lipid droplets by inhibiting DNA repair responses, resulting in the activation of the type-I IFN pathway and scavenger receptor-A1 (SR-A1)-mediated lipid droplet formation. Bacterial urease C (UreC, Rv1850) inhibits host DNA repair by interacting with RuvB-like protein 2 (RUVBL2) and impeding the formation of the RUVBL1-RUVBL2-RAD51 DNA repair complex. The suppression of this repair pathway increases the abundance of micronuclei that trigger the cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes (STING) pathway and subsequent interferon-β (IFN-β) production. UreC-mediated activation of the IFN-β pathway upregulates the expression of SR-A1 to form lipid droplets that facilitate Mtb replication. UreC inhibition via a urease inhibitor impaired Mtb growth within macrophages and in vivo. Thus, our findings identify mechanisms by which Mtb triggers a cascade of cellular events that establish a nutrient-rich replicative niche.","PeriodicalId":93926,"journal":{"name":"Cell host & microbe","volume":" ","pages":"1820-1836.e10"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An atlas of continuous adaptive evolution in endemic human viruses. 地方性人类病毒持续适应性进化图谱。

Cell host & microbe Pub Date : 2023-11-08 Epub Date: 2023-10-25 DOI: 10.1016/j.chom.2023.09.012

Kathryn E Kistler, Trevor Bedford

引用次数: 0

Molecular basis for inhibition of type III-B CRISPR-Cas by an archaeal viral anti-CRISPR protein. 古菌病毒抗CRISPR蛋白抑制III-B型CRISPR-Cas的分子基础。

Cell host & microbe Pub Date : 2023-11-08 Epub Date: 2023-10-30 DOI: 10.1016/j.chom.2023.10.003

Jinzhong Lin, Lauge Alfastsen, Yuvaraj Bhoobalan-Chitty, Xu Peng

引用次数: 1

Bug in the code: TB blocks DNA repair. 代码中的错误：TB阻碍DNA修复。

Cell host & microbe Pub Date : 2023-11-08 DOI: 10.1016/j.chom.2023.10.012

Bala T S A Madduri, Samantha L Bell

引用次数: 0