Asian journal of andrology最新文献_第8页

Integrated seminal plasma metabolomics and lipidomics profiling highlight distinctive signature of varicocele patients with male infertility. 精浆代谢组学和脂质组学综合分析突出了精索静脉曲张患者男性不育症的独特特征。

Asian journal of andrology Pub Date : 2025-04-08 DOI: 10.4103/aja2024113

Jing-Di Zhang, Xiao-Gang Li, Rong-Rong Wang, Xin-Xin Feng, Si-Yu Wang, Hai Wang, Yu-Tao Wang, Hong-Jun Li, Yong-Zhe Li, Ye Guo

{"title":"Integrated seminal plasma metabolomics and lipidomics profiling highlight distinctive signature of varicocele patients with male infertility.","authors":"Jing-Di Zhang, Xiao-Gang Li, Rong-Rong Wang, Xin-Xin Feng, Si-Yu Wang, Hai Wang, Yu-Tao Wang, Hong-Jun Li, Yong-Zhe Li, Ye Guo","doi":"10.4103/aja2024113","DOIUrl":"https://doi.org/10.4103/aja2024113","url":null,"abstract":"Varicocele (VC) is a common cause of male infertility, yet there is a lack of molecular information for VC-associated male infertility. This study investigated alterations in the seminal plasma metabolomic and lipidomic profiles of infertile male VC patients. Twenty infertile males with VC and twenty-three age-matched healthy controls (HCs) were recruited from Peking Union Medical College Hospital (Beijing, China) between October 2019 and April 2021. Untargeted metabolite and lipid profiles from seminal plasma were analyzed using mass spectrometry. Four hundred and seventy-six metabolites and seventeen lipids were significantly different in infertile male VC patients compared to HCs. The top enriched pathways among these significantly different metabolites are protein digestion and absorption, aminoacyl-transfer RNA (tRNA) biosynthesis, and biosynthesis of amino acids. Different key lipid species, including triglyceride (TG), diacylglycerol (DG), ceramides (Cer), and phosphatidylserine (PS), varied between VC and HC groups. The distinct metabolites and lipids were moderately correlated. DL-3-phenyllactic acid is a potential diagnostic biomarker for VC-related male infertility (area under the curve [AUC] = 0.893), positively correlating with sperm count, concentration, and motility. Furthermore, DL-3-phenyllactic acid is the only metabolite shared by all four comparisons (VC vs HC, VC-induced oligoasthenospermia [OAS] vs VC-induced asthenospermia [AS], OAS vs HC, and AS vs HC). DL-3-phenyllactic acid significantly decreased in OAS than AS. Metabolite-targeting gene analysis revealed carbonic anhydrase 9 (CA9) might be the strongest candidate associated with the onset and severity of VC. The seminal plasma metabolite and lipid profiles of infertile males with VC differ significantly from those of HCs. DL-3-phenyllactic acid could be a promising biomarker.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143805081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted gene silencing in mouse testicular Sertoli and Leydig cells using adeno-associated virus vectors. 利用腺相关病毒载体对小鼠睾丸支持细胞和间质细胞进行靶向基因沉默。

Asian journal of andrology Pub Date : 2025-03-21 DOI: 10.4103/aja2024120

Jing Pang, Mao-Xing Xu, Xiao-Yu Wang, Xu Feng, Yi-Man Duan, Xiao-Yan Zheng, Yu-Qian Chen, Wen Yin, Ying Liu, Ju-Xue Li

{"title":"Targeted gene silencing in mouse testicular Sertoli and Leydig cells using adeno-associated virus vectors.","authors":"Jing Pang, Mao-Xing Xu, Xiao-Yu Wang, Xu Feng, Yi-Man Duan, Xiao-Yan Zheng, Yu-Qian Chen, Wen Yin, Ying Liu, Ju-Xue Li","doi":"10.4103/aja2024120","DOIUrl":"https://doi.org/10.4103/aja2024120","url":null,"abstract":"Abstract: Researchers commonly use cyclization recombination enzyme/locus of X-over P1 (Cre/loxP) technology-based conditional gene knockouts of model mice to investigate the functional roles of genes of interest in Sertoli and Leydig cells within the testis. However, the shortcomings of these genetic tools include high costs, lengthy experimental periods, and limited accessibility for researchers. Therefore, exploring alternative gene silencing techniques is of great practical value. In this study, we employed adeno-associated virus (AAV) as a vector for gene silencing in Sertoli and Leydig cells. Our findings demonstrated that AAV serotypes 1, 8, and 9 exhibited high infection efficiency in both types of testis cells. Importantly, we discovered that all three AAV serotypes exhibited exquisite specificity in targeting Sertoli cells via tubular injection while demonstrating remarkable selectivity in targeting Leydig cells via interstitial injection. We achieved cell-specific knockouts of the steroidogenic acute regulatory (Star) and luteinizing hormone/human chorionic gonadotropin receptor (Lhcgr) genes in Leydig cells, but not in Sertoli cells, using AAV9-single guide RNA (sgRNA)-mediated gene editing in Rosa26-LSL-Cas9 mice. Knockdown of androgen receptor (Ar) gene expression in Sertoli cells of wild-type mice was achieved via tubular injection of AAV9-short hairpin RNA (shRNA)-mediated targeting. Our findings offer technical approaches for investigating gene function in Sertoli and Leydig cells through AAV9-mediated gene silencing.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm tRNA-derived fragments expression is potentially linked to abstinence-related improvement of sperm quality. 精子trna衍生片段的表达可能与禁欲相关的精子质量改善有关。

Asian journal of andrology Pub Date : 2025-03-18 DOI: 10.4103/aja2024123

Xi-Ren Ji, Rui-Jun Wang, Zeng-Hui Huang, Hui-Lan Wu, Xiu-Hai Huang, Hao Bo, Ge Lin, Wen-Bing Zhu, Chuan Huang

{"title":"Sperm tRNA-derived fragments expression is potentially linked to abstinence-related improvement of sperm quality.","authors":"Xi-Ren Ji, Rui-Jun Wang, Zeng-Hui Huang, Hui-Lan Wu, Xiu-Hai Huang, Hao Bo, Ge Lin, Wen-Bing Zhu, Chuan Huang","doi":"10.4103/aja2024123","DOIUrl":"https://doi.org/10.4103/aja2024123","url":null,"abstract":"Abstract: Recent studies have shown that shorter periods of ejaculatory abstinence may enhance certain sperm parameters, but the molecular mechanisms underlying these improvements are still unclear. This study explored whether reduced abstinence periods could improve semen quality, particularly for use in assisted reproductive technologies (ART). We analyzed semen samples from men with normal sperm counts (n = 101) and those with low sperm motility or concentration (n = 53) after 3-7 days of abstinence and then after 1-3 h of abstinence, obtained from the Reproductive & Genetic Hospital of CITIC-Xiangya (Changsha, China). Physiological and biochemical sperm parameters were evaluated, and the dynamics of transfer RNA (tRNA)-derived fragments (tRFs) were analyzed using deep RNA sequencing in five consecutive samples from men with normal sperm counts. Our results revealed significant improvement in sperm motility and a decrease in the DNA fragmentation index after the 1- to 3-h abstinence period. Additionally, we identified 245 differentially expressed tRFs, and the mitogen-activated protein kinase (MAPK) signaling pathway was the most enriched. Further investigations showed significant changes in tRF-Lys-TTT and its target gene mitogen-activated protein kinase kinase 2 (MAP2K2), which indicates a role of tRFs in improving sperm function. These findings provide new insights into how shorter abstinence periods influence sperm quality and suggest that tRFs may serve as biomarkers for male fertility. This research highlights the potential for optimizing ART protocols and improving reproductive outcomes through molecular approaches that target sperm function.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of testosterone synthesis by circadian clock genes and its research progress in male diseases. 生理时钟基因调控睾酮合成及其在男性疾病中的研究进展。

Asian journal of andrology Pub Date : 2025-03-18 DOI: 10.4103/aja20258

Gang Ning, Bo-Nan Li, Hui Wu, Ruo-Bing Shi, A-Jian Peng, Hao-Yu Wang, Xing Zhou

引用次数: 0

Impact of physical activity on semen quality: a review of current evidence. 体育活动对精液质量的影响：对现有证据的回顾。

Asian journal of andrology Pub Date : 2025-03-14 DOI: 10.4103/aja20252

Jing Chen, Jin-Ming Guo, Bang-Jian Jiang, Fan-Yuan Sun, Yong-Cun Qu

引用次数: 0

Exploration of evaluation criteria based on the biological variation in the external quality assessment for basic semen analysis in China. 中国精液基础分析外部质量评价中基于生物学变异的评价标准探讨。

Asian journal of andrology Pub Date : 2025-03-11 DOI: 10.4103/aja2024118

Xi-Yan Wu, Jin-Chun Lu, Xin-Hua Peng, Jing-Liang He, Dao Wang, Cong-Ling Dai, Wen-Bing Zhu, Gang Liu, Wei-Na Li

{"title":"Exploration of evaluation criteria based on the biological variation in the external quality assessment for basic semen analysis in China.","authors":"Xi-Yan Wu, Jin-Chun Lu, Xin-Hua Peng, Jing-Liang He, Dao Wang, Cong-Ling Dai, Wen-Bing Zhu, Gang Liu, Wei-Na Li","doi":"10.4103/aja2024118","DOIUrl":"https://doi.org/10.4103/aja2024118","url":null,"abstract":"Abstract: This study explores whether the current external quality assessment (EQA) level and acceptable bias for basic semen analysis in China are clinically useful. We collected data of semen EQA from Andrology laboratories in the Hunan Province (China) in 2022 and searched for data in the published literature from January 2000 to December 2023 in China. On the basis of these data, we analyzed the coefficients of variation and acceptable biases of different quality control materials for basic semen analysis through robust statistics. We compared these findings with quality specifications based on biological variation from optimal, desirable, and minimum levels of bias to seek a unified and more suitable semen EQA bias evaluation standard for China's national conditions. Different sources of semen quality control material exhibited considerable variation in acceptable biases among laboratories, ranging from 8.2% to 56.9%. A total of 50.0% of the laboratories met the minimum quality specifications for progressive motility (PR), whereas 100.0% and 75.0% of laboratories met only the minimum quality specifications for sperm concentration and total motility (nonprogressive [NP] + PR), respectively. The Z value for sperm concentration and PR+NP was equivalent to the desirable performance specification, whereas the Z value for PR was equivalent only to the minimum performance specification. This study highlights the feasibility of operating external quality assessment schemes for basic semen analysis using quality specifications based on biological variation. These specifications should be unified among external quality control (EQC) centers based on biological variation.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cystic fibrosis-causing variants in Chinese patients with congenital absence of the vas deferens: a cohort and meta-analysis. 中国先天性输精管缺失患者的囊性纤维化变异：一项队列和荟萃分析

Asian journal of andrology Pub Date : 2025-03-11 DOI: 10.4103/aja2024124

Yi Lu, Jing Wang, Zhong-Lin Cai, Teng-Yan Li, Hong-Jun Li, Bin-Bin Wang

{"title":"Cystic fibrosis-causing variants in Chinese patients with congenital absence of the vas deferens: a cohort and meta-analysis.","authors":"Yi Lu, Jing Wang, Zhong-Lin Cai, Teng-Yan Li, Hong-Jun Li, Bin-Bin Wang","doi":"10.4103/aja2024124","DOIUrl":"https://doi.org/10.4103/aja2024124","url":null,"abstract":"Abstract: Individuals with congenital absence of the vas deferens (CAVD) may transmit cystic fibrosis (CF)-causing variants of the cystic fibrosis transmembrane conductance regulator (CFTR) gene to their offspring through assisted reproductive technology (ART). We aimed to delineate the spectrum and estimate the prevalence of CF-causing variants in Chinese individuals with CAVD through a cohort analysis and meta-analysis. CFTR was sequenced in 145 Chinese individuals with CAVD. CFTR variants were classified as CF-causing or non-CF-causing variants regarding clinical significance. A comprehensive genotype analysis was performed in Chinese individuals with CAVD, incorporating previous studies and our study cohort. The prevalence of CF-causing variants was estimated through meta-analysis. In our cohort, 56 different CFTR variants were identified in 108 (74.5%) patients. Twenty variants were categorized as CF-causing and were detected in 28 (19.3%) patients. A comprehensive genotype analysis of 867 patients identified 174 different CFTR variants. Sixty-four were classified as CF-causing variants, 56.3% of which had not been previously reported in Chinese patients with CF. Meta-analysis showed that 14.8% (95% confidence interval [CI]: 11.0%-18.9%) CAVD cases harbored one CF-causing variant, and 68.6% (95% CI: 65.1%-72.0%) CAVD cases carried at least one CFTR variant. Our study underscores the urgent need for extensive CFTR screening, including sequencing of whole exons and flanking regions and detection of large rearrangements and deep intronic CF-causing variants, in Chinese individuals with CAVD before undergoing ART. The established CF-causing variants spectrum may aid in the development of genetic counseling strategies and preimplantation diagnosis to prevent the birth of a child with CF.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel homozygous splicing mutation in AK7 associated with multiple morphological abnormalities of the sperm flagella. 与精子鞭毛多种形态异常有关的 AK7 基因新型同源剪接突变。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-09-17 DOI: 10.4103/aja202479

Thomas Greither, Holger Herlyn

引用次数: 0

High sperm DNA fragmentation - finding a needle in the haystack: tips on selecting the best sperm for ICSI and ART. 精子 DNA 碎片率高--大海捞针：为 ICSI 和 ART 挑选最佳精子的技巧。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-09-03 DOI: 10.4103/aja202451

Elisabetta Baldi, Giovanni M Colpi, Zhong-Wei Huang, Balasingam Balagobi, Florence Boitrelle, Rupin Shah, Ashok Agarwal

引用次数: 0

Exploring the clinical implications of novel SRD5A2 variants in 46,XY disorders of sex development. 探索新型 SRD5A2 变异对 46,XY 性发育障碍的临床影响。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-09-24 DOI: 10.4103/aja202469

Yu Mao, Jian-Mei Huang, Yu-Wei Chen-Zhang, He Lin, Yu-Huan Zhang, Ji-Yang Jiang, Xue-Mei Wu, Ling Liao, Yun-Man Tang, Ji-Yun Yang

{"title":"Exploring the clinical implications of novel SRD5A2 variants in 46,XY disorders of sex development.","authors":"Yu Mao, Jian-Mei Huang, Yu-Wei Chen-Zhang, He Lin, Yu-Huan Zhang, Ji-Yang Jiang, Xue-Mei Wu, Ling Liao, Yun-Man Tang, Ji-Yun Yang","doi":"10.4103/aja202469","DOIUrl":"10.4103/aja202469","url":null,"abstract":"Abstract: This study was conducted retrospectively on a cohort of 68 patients with steroid 5 α-reductase 2 (SRD5A2) deficiency and 46,XY disorders of sex development (DSD). Whole-exon sequencing revealed 28 variants of SRD5A2 , and further analysis identified seven novel mutants. The preponderance of variants was observed in exon 1 and exon 4, specifically within the nicotinamide adenine dinucleotide phosphate (NADPH)-binding region. Among the entire cohort, 53 patients underwent initial surgery at Sichuan Provincial People's Hospital (Chengdu, China). The external genitalia scores (EGS) of these participants varied from 2.0 to 11.0, with a mean of 6.8 (standard deviation [s.d.]: 2.5). Thirty patients consented to hormone testing. Their average testosterone-to-dihydrotestosterone (T/DHT) ratio was 49.3 (s.d.: 23.4). Genetic testing identified four patients with EGS scores between 6 and 9 as having this syndrome; and their T/DHT ratios were below the diagnostic threshold. Furthermore, assessments conducted using the crystal structure of human SRD5A2 have provided insights into the potential pathogenic mechanisms of these novel variants. These mechanisms include interference with NADPH binding (c.356G>C, c.365A>G, c.492C>G, and c.662T>G) and destabilization of the protein structure (c.727C>T). The c.446-1G>T and c.380delG variants were verified to result in large alterations in the transcripts. Seven novel variations were identified, and the variant database for the SRD5A2 gene was expanded. These findings contribute to the progress of diagnostic and therapeutic approaches for individuals with SRD5A2 deficiency.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"211-218"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0