American journal of physiology. Renal physiology最新文献

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VCAM-1 mediates proximal tubule-immune cell cross talk in failed tubule recovery during AKI-to-CKD transition. VCAM-1介导近端小管-免疫细胞串联,导致 AKI 向 CKD 过渡期间小管恢复失败。
American journal of physiology. Renal physiology Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1152/ajprenal.00076.2024
Isabel Melchinger, Kailin Guo, Xiaoxu Li, Jiankan Guo, Lloyd G Cantley, Leyuan Xu
{"title":"VCAM-1 mediates proximal tubule-immune cell cross talk in failed tubule recovery during AKI-to-CKD transition.","authors":"Isabel Melchinger, Kailin Guo, Xiaoxu Li, Jiankan Guo, Lloyd G Cantley, Leyuan Xu","doi":"10.1152/ajprenal.00076.2024","DOIUrl":"10.1152/ajprenal.00076.2024","url":null,"abstract":"<p><p>Studies in animal models have suggested a linkage between the inflammatory response to injury and subsequent nephron loss during the acute kidney injury (AKI) to chronic kidney disease (CKD) transition. Failure of normal repair during the CKD transition correlates with de novo expression of vascular cell adhesion protein-1 (VCAM-1) by a subset of injured proximal tubule cells. This study identified the role of VCAM-1 expression in promoting the failed repair state. Single-cell transcriptome analysis of patients with AKI and CKD and whole kidney RNA and protein analyses of mouse models of CKD confirmed a marked increase of VCAM-1 expression in the proximal tubules of injured kidneys. In immortalized mouse proximal tubular cells and primary cultured renal cells (PCRCs), VCAM-1 expression was induced by proinflammatory cytokines including tumor necrosis factor (TNF)-α and interleukin (IL)-1β. Analyses of bulk RNA sequencing of TNF-α-treated primary cultured renal cells or pseudo-bulk RNA sequencing of biopsies from Kidney Precision Medicine Project datasets indicated activation of NF-κB and an enrichment of inflammatory response and cell adhesion pathways in VCAM-1-positive cells. Pharmacological inhibition of NF-κB signaling or genetic deletion of myeloid differentiation factor 88 and TIR domain-containing adapter-inducing interferon-β suppressed TNF-α- and IL-1β-induced VCAM-1 expression in vitro. TNF-α stimulation or overexpression of VCAM-1 significantly increased splenocyte adhesion to the mouse proximal tubular monolayer in culture. These results demonstrate that persistence of proinflammatory cytokines after AKI can induce NF-κB-dependent VCAM-1 expression by proximal tubule cells, mediating increased immune cell adhesion to the tubule and thus promoting further tubule injury and greater risk of progression from AKI to CKD.<b>NEW & NOTEWORTHY</b> We demonstrated the induction of VCAM-1 and its biological function in proximal tubules. We found that proinflammatory cytokines (TNF-α and IL-1β) significantly induced VCAM-1 expression via NF-κB signaling pathway. TNF-α treatment or overexpression of VCAM-1 in immortalized MPT cells increased CD45<sup>+</sup> splenocyte adhesion. Pharmacological inhibition of NF-κB or genetic deletion of Vcam1 suppressed TNF-α-induced splenocyte adhesion in vitro, suggesting that VCAM-1 mediates proximal tubular-immune cell cross talk in failed tubule recovery during AKI-to-CKD transition.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F610-F622"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11483080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Distal convoluted tubule-specific disruption of the COP9 signalosome but not its regulatory target cullin 3 causes tubular injury. 远端曲细管特异性地破坏 COP9 信号体而非其调控靶标 Cullin 3 会导致曲细管损伤。
American journal of physiology. Renal physiology Pub Date : 2024-10-01 Epub Date: 2024-08-29 DOI: 10.1152/ajprenal.00138.2024
Yujiro Maeoka, Tanner Bradford, Xiao-Tong Su, Avika Sharma, Chao-Ling Yang, David H Ellison, James A McCormick, Ryan J Cornelius
{"title":"Distal convoluted tubule-specific disruption of the COP9 signalosome but not its regulatory target cullin 3 causes tubular injury.","authors":"Yujiro Maeoka, Tanner Bradford, Xiao-Tong Su, Avika Sharma, Chao-Ling Yang, David H Ellison, James A McCormick, Ryan J Cornelius","doi":"10.1152/ajprenal.00138.2024","DOIUrl":"10.1152/ajprenal.00138.2024","url":null,"abstract":"<p><p>The disease familial hyperkalemic hypertension (FHHt; also known as Gordon syndrome) is caused by aberrant accumulation of with-no-lysine kinase (WNK4) activating the NaCl cotransporter (NCC) in the distal convoluted tubule (DCT) of the kidney. Mutations in cullin 3 (CUL3) cause FHHt by disrupting interaction with the deneddylase COP9 signalosome (CSN). Deletion of <i>Cul3</i> or <i>Jab1</i> (the catalytically active CSN subunit) along the entire nephron causes a partial FHHt phenotype with activation of the WNK4-STE20/SPS1-related proline/alanine-rich kinase (SPAK)-NCC pathway. However, progressive kidney injury likely prevents hypertension, hyperkalemia, and hyperchloremic metabolic acidosis associated with FHHt. We hypothesized that DCT-specific deletion would more closely model the disease. We used <i>Slc12a3</i>-Cre-ERT2 mice to delete <i>Cul3</i> (DCT-<i>Cul3</i><sup>-/-</sup>) or <i>Jab1</i> (DCT-<i>Jab1</i><sup>-/-</sup>) only in the DCT and examined the mice after short- and long-term deletion. Short-term DCT-specific knockout of both <i>Cul3</i> and <i>Jab1</i> mice caused elevated WNK4, pSPAK<sup>S373</sup>, and pNCC<sup>T53</sup> abundance. However, neither model demonstrated changes in plasma K<sup>+</sup>, Cl<sup>-</sup>, or total CO<sub>2</sub>, even though no injury was present. Long-term DCT-<i>Jab1</i><sup>-/-</sup> mice showed significantly lower NCC and parvalbumin abundance and a higher abundance of kidney injury molecule-1, a marker of proximal tubule injury. No injury or reduction in NCC or parvalbumin was observed in long-term DCT-<i>Cul3</i><sup>-/-</sup> mice. In summary, the prevention of injury outside the DCT did not lead to a complete FHHt phenotype despite activation of the WNK4-SPAK-NCC pathway, possibly due to insufficient NCC activation. Chronically, only DCT-<i>Jab1</i><sup>-/-</sup> mice developed tubule injury and atrophy of the DCT, suggesting a direct JAB1 effect or dysregulation of other cullins as mechanisms for injury.<b>NEW & NOTEWORTHY</b> CUL3 degrades WNK4, which prevents activation of NCC in the DCT. CSN regulation of CUL3 is impaired in the disease FHHt, causing accumulation of WNK4. Short-term DCT-specific disruption of CUL3 or the CSN in mice resulted in activation of the WNK4-SPAK-NCC pathway but not hyperkalemic metabolic acidosis found in FHHt. Tubule injury was observed only after long-term CSN disruption. The data suggest that disruption of other cullins may be the cause for the injury.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F667-F682"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11483082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First Author Highlights. 第一作者亮点。
American journal of physiology. Renal physiology Pub Date : 2024-10-01 DOI: 10.1152/ajprenal.2024.327.4.AU
{"title":"First Author Highlights.","authors":"","doi":"10.1152/ajprenal.2024.327.4.AU","DOIUrl":"https://doi.org/10.1152/ajprenal.2024.327.4.AU","url":null,"abstract":"","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":"327 4","pages":"i"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulating distal nephron functions and salt sensitivity. 调节远端肾小管功能和盐敏感性
American journal of physiology. Renal physiology Pub Date : 2024-10-01 Epub Date: 2024-07-18 DOI: 10.1152/ajprenal.00103.2024
Kohei Ueda, Tatsuo Shimosawa
{"title":"Regulating distal nephron functions and salt sensitivity.","authors":"Kohei Ueda, Tatsuo Shimosawa","doi":"10.1152/ajprenal.00103.2024","DOIUrl":"10.1152/ajprenal.00103.2024","url":null,"abstract":"<p><p>This review highlights the molecular basis of salt sensitivity in hypertension, with a focus on the regulation of sodium transport in the distal nephron. Sodium reabsorption in this region is often linked to the actions of aldosterone, although in recent years numerous findings have been reported on the aldosterone-independent pathway of acquiring salt sensitivity by potassium deficiency or potassium loading. The key to this discussion is the interplay, through extracellular potassium concentration, between the first part of the tubules expressing the Na<sup>+</sup>-Cl<sup>-</sup> cotransporter (NCC) and the second part expressing the epithelial Na<sup>+</sup> channel (ENaC). The molecular pathways such as with-no-lysine 1 (WNK)-STE20/SPS1-related proline-alanine-rich kinase (SPAK)/oxidative stress-responsive kinase 1 (OSR1) signaling, Kelch-like family member 3 (KLHL3)-cullin 3 (CUL3) complex, protein phosphatases, and mechanistic target of rapamycin complex 2 (mTORC2)-Nedd4L pathway are described as the mechanism by which salt sensitivity on blood pressure is acquired in response to changes in physiological conditions including potassium depletion or loading. This review highlights the potential for targeting these molecular pathways to develop novel therapeutic strategies for the treatment of salt-sensitive hypertension, the mechanism of which remains to be elucidated.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F566-F580"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141725274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxysterol-binding protein-like 7 deficiency leads to ER stress-mediated apoptosis in podocytes and proteinuria. 羟基固醇结合蛋白 7 缺乏症会导致ER应激介导的荚膜细胞凋亡和蛋白尿。
American journal of physiology. Renal physiology Pub Date : 2024-09-01 Epub Date: 2024-07-04 DOI: 10.1152/ajprenal.00319.2023
Joanne Duara, Maria Torres, Margaret Gurumani, Judith Molina David, Rachel Njeim, Jin-Ju Kim, Alla Mitrofanova, Mengyuan Ge, Alexis Sloan, Janina Müller-Deile, Mario Schiffer, Sandra Merscher, Alessia Fornoni
{"title":"Oxysterol-binding protein-like 7 deficiency leads to ER stress-mediated apoptosis in podocytes and proteinuria.","authors":"Joanne Duara, Maria Torres, Margaret Gurumani, Judith Molina David, Rachel Njeim, Jin-Ju Kim, Alla Mitrofanova, Mengyuan Ge, Alexis Sloan, Janina Müller-Deile, Mario Schiffer, Sandra Merscher, Alessia Fornoni","doi":"10.1152/ajprenal.00319.2023","DOIUrl":"10.1152/ajprenal.00319.2023","url":null,"abstract":"<p><p>Chronic kidney disease (CKD) is associated with renal lipid dysmetabolism among a variety of other pathways. We recently demonstrated that oxysterol-binding protein-like 7 (OSBPL7) modulates the expression and function of ATP-binding cassette subfamily A member 1 (ABCA1) in podocytes, a specialized type of cell essential for kidney filtration. Drugs that target OSBPL7 lead to improved renal outcomes in several experimental models of CKD. However, the role of OSBPL7 in podocyte injury remains unclear. Using mouse models and cellular assays, we investigated the influence of OSBPL7 deficiency on podocytes. We demonstrated that reduced renal OSBPL7 levels as observed in two different models of experimental CKD are linked to increased podocyte apoptosis, primarily mediated by heightened endoplasmic reticulum (ER) stress. Although as expected, the absence of OSBPL7 also resulted in lipid dysregulation (increased lipid droplets and triglycerides content), OSBPL7 deficiency-related lipid dysmetabolism did not contribute to podocyte injury. Similarly, we demonstrated that the decreased autophagic flux we observed in OSBPL7-deficient podocytes was not the mechanistic link between OSBPL7 deficiency and apoptosis. In a complementary zebrafish model, osbpl7 knockdown was sufficient to induce proteinuria and morphological damage to the glomerulus, underscoring its physiological relevance. Our study sheds new light on the mechanistic link between OSBPL7 deficiency and podocyte injury in glomerular diseases associated with CKD, and it strengthens the role of OSBPL7 as a novel therapeutic target.<b>NEW & NOTEWORTHY</b> OSBPL7 and ER stress comprise a central mechanism in glomerular injury. This study highlights a crucial link between OSBPL7 deficiency and ER stress in CKD. OSBPL7 deficiency causes ER stress, leading to podocyte apoptosis. There is a selective effect on lipid homeostasis in that OSBPL7 deficiency affects lipid homeostasis, altering cellular triglyceride but not cholesterol content. The interaction of ER stress and apoptosis supports that ER stress, not reduced autophagy, is the main driver of apoptosis in OSBPL7-deficient podocytes.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F340-F350"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigating FSGS-like injury in zebrafish larvae by nifurpirinol: efficacy and molecular insight. 用 Nifurpirinol 研究斑马鱼幼体 FSGS 类损伤:疗效和分子洞察力
American journal of physiology. Renal physiology Pub Date : 2024-09-01 Epub Date: 2024-07-11 DOI: 10.1152/ajprenal.00116.2024
Marianne Klawitter, Francescapaola Mattias, Felix Kliewe, Elke Hammer, Uwe Völker, Stefan Simm, Florian Siegerist, Sophie Daniel, Maximilian Schindler, Nicole Endlich
{"title":"Investigating FSGS-like injury in zebrafish larvae by nifurpirinol: efficacy and molecular insight.","authors":"Marianne Klawitter, Francescapaola Mattias, Felix Kliewe, Elke Hammer, Uwe Völker, Stefan Simm, Florian Siegerist, Sophie Daniel, Maximilian Schindler, Nicole Endlich","doi":"10.1152/ajprenal.00116.2024","DOIUrl":"10.1152/ajprenal.00116.2024","url":null,"abstract":"<p><p>Identifying effective drugs for focal segmental glomerulosclerosis (FSGS) treatment holds significant importance. Our high-content drug screening on zebrafish larvae relies on nitroreductase/metronidazole (NTR/MTZ)-induced podocyte ablation to generate FSGS-like injury. A crucial factor for successful drug screenings is minimizing variability in injury induction. For this, we introduce nifurpirinol (NFP) as a more reliable prodrug for targeted podocyte depletion. NFP showed a 2.3-fold increase in efficiency at concentrations 1,600-fold lower compared with MTZ-mediated injury induction. Integration into the screening workflow validated its suitability for the high-content drug screening. The presence of crucial FSGS hallmarks, such as podocyte foot process effacement, proteinuria, and activation of parietal epithelial cells, was observed. After the isolation of the glomeruli from the larvae, we identified essential pathways by proteomic analysis. This study shows that NFP serves as a highly effective prodrug to induce the FSGS-like disease in zebrafish larvae and is well-suited for a high-content drug screening to identify new candidates for the treatment of FSGS.<b>NEW & NOTEWORTHY</b> This research investigated the use of nifurpirinol in nanomolar amounts as a prodrug to reliably induce focal segmental glomerulosclerosis (FSGS)-like damage in transgenic zebrafish larvae. Through proteomic analysis of isolated zebrafish glomeruli, we were further able to identify proteins that are significantly regulated after the manifestation of FSGS. These results are expected to expand our knowledge of the pathomechanism of FSGS.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F463-F475"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inducible deletion of the prostaglandin EP3 receptor in kidney tubules of male and female mice has no major effect on water homeostasis. 雌雄小鼠肾小管中前列腺素 EP3 受体的诱导性缺失对水稳态无重大影响
American journal of physiology. Renal physiology Pub Date : 2024-09-01 Epub Date: 2024-07-04 DOI: 10.1152/ajprenal.00146.2024
Cristina Esteva-Font, Frank Geurts, Toke P K Hansen, Ewout J Hoorn, Robert A Fenton
{"title":"Inducible deletion of the prostaglandin EP3 receptor in kidney tubules of male and female mice has no major effect on water homeostasis.","authors":"Cristina Esteva-Font, Frank Geurts, Toke P K Hansen, Ewout J Hoorn, Robert A Fenton","doi":"10.1152/ajprenal.00146.2024","DOIUrl":"10.1152/ajprenal.00146.2024","url":null,"abstract":"<p><p>The prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) receptor EP3 has been detected in the thick ascending limb (TAL) and the collecting duct of the kidney, where its actions are proposed to inhibit water reabsorption. However, EP3 is also expressed in other cell types, including vascular endothelial cells. The aim here was to determine the contribution of EP3 in renal water handling in male and female adult mice by phenotyping a novel mouse model with doxycycline-dependent deletion of EP3 throughout the kidney tubule (EP3<sup>-/-</sup> mice). RNAscope demonstrated that EP3 was highly expressed in the cortical and medullary TAL of adult mice. Compared with controls EP3 mRNA expression was reduced by >80% in whole kidney (RT-qPCR) and nondetectable (RNAscope) in renal tubules of EP3<sup>-/-</sup> mice. Under basal conditions, there were no significant differences in control and EP3<sup>-/-</sup> mice of both sexes in food and water intake, body weight, urinary output, or clinical biochemistries. No differences were detectable between genotypes in handling of an acute water load or in their response to the vasopressin analog 1-deamino-8-d-arginine-vasopressin (dDAVP). No differences in water handling were observed when PGE<sub>2</sub> production was enhanced using 1% NaCl load. Expression of proteins involved in kidney water handling was not different between genotypes. This study demonstrates that renal tubular EP3 is not essential for body fluid homeostasis in males or females, even when PGE<sub>2</sub> levels are high. The mouse model is a novel tool for examining the role of EP3 in kidney function independently of potential developmental abnormalities or systemic effects.<b>NEW & NOTEWORTHY</b> The prostanoid EP3 receptor is proposed to play a key role in the kidney tubule and antagonize the effects of vasopressin on aquaporin-mediated water reabsorption. Here, we phenotyped a kidney tubule-specific inducible knockout mouse model of the EP3 receptor. Our major finding is that, even under physiological stress, tubular EP3 plays no detectable role in renal water or solute handling. This suggests that other EP receptors must be important for renal salt and water handling.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F504-F518"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TRPV1 and mast cell involvement in repeated variate stress-induced urinary bladder dysfunction in adult female mice. TRPV1和肥大细胞参与反复变异应激(RVS)诱发成年雌性小鼠膀胱功能障碍的过程
American journal of physiology. Renal physiology Pub Date : 2024-09-01 Epub Date: 2024-07-11 DOI: 10.1152/ajprenal.00125.2024
Amanda B Sidwell, Beatrice M Girard, Susan E Campbell, Margaret A Vizzard
{"title":"TRPV1 and mast cell involvement in repeated variate stress-induced urinary bladder dysfunction in adult female mice.","authors":"Amanda B Sidwell, Beatrice M Girard, Susan E Campbell, Margaret A Vizzard","doi":"10.1152/ajprenal.00125.2024","DOIUrl":"10.1152/ajprenal.00125.2024","url":null,"abstract":"<p><p>The etiology of interstitial cystitis/bladder pain syndrome (IC/BPS) is unknown but likely multifactorial. IC/BPS symptoms can be exacerbated by psychological stress, but underlying mechanisms remain to be defined. Transient receptor potential vanilloid 1 (TRPV1) channels, expressed on nerve fibers, have been implicated in bladder dysfunction and colonic hypersensitivity with stress in rodents. Histamine/H1R activation of TRPV1<sup>+</sup> nerves increases bladder afferent fiber sensitivity to distension. TRPV1 channels are also expressed on mast cells, previously implicated in contributing to IC/BPS etiology and symptoms. We have examined the contribution of TRPV1 and mast cells to bladder dysfunction after repeated variate stress (RVS). RVS increased (<i>P</i> ≤ 0.05) serum and fecal corticosterone expression and induced anxiety-like behavior in wild-type (WT) mice. Intravesical instillation of the selective TRPV1 antagonist capsazepine (CPZ) rescued RVS-induced bladder dysfunction in WT mice. <i>Trpv1</i> knockout (KO) mice did not increase voiding frequency with RVS and did not exhibit increased serum corticosterone expression despite exhibiting anxiety-like behavior. Mast cell-deficient mice (B6.Cg-<i>Kit</i><sup>w-sh</sup>) failed to demonstrate RVS-induced increased voiding frequency or serum corticosterone expression, whereas control (no stress) mast cell-deficient mice had similar functional bladder capacity to WT mice. TRPV1 protein expression was significantly increased in the rostral lumbar (L1-L2) spinal cord and dorsal root ganglia (DRG) in WT mice exposed to RVS, but no changes were observed in lumbosacral (L6-S1) spinal segments or DRG. These studies demonstrated TRPV1 and mast cell involvement in RVS-induced increased voiding frequency and suggest that TRPV1 and mast cells may be useful targets to mitigate stress-induced urinary bladder dysfunction.<b>NEW & NOTEWORTHY</b> Using pharmacological tools and transgenic mice in a repeated variate stress (RVS) model in female mice, we demonstrate that transient receptor potential vanilloid 1 (TRPV1) and mast cells contribute to the increased voiding frequency observed following RVS. TRPV1 and mast cells should continue to be considered as targets to improve bladder function in stress-induced bladder dysfunction.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F476-F488"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The mineralocorticoid receptor in diabetic kidney disease. 糖尿病肾病中的矿物质皮质激素受体。
American journal of physiology. Renal physiology Pub Date : 2024-09-01 Epub Date: 2024-07-18 DOI: 10.1152/ajprenal.00135.2024
Guanghong Jia, Guido Lastra, Brian P Bostick, Nihay LahamKaram, Johanna P Laakkonen, Seppo Ylä-Herttuala, Adam Whaley-Connell
{"title":"The mineralocorticoid receptor in diabetic kidney disease.","authors":"Guanghong Jia, Guido Lastra, Brian P Bostick, Nihay LahamKaram, Johanna P Laakkonen, Seppo Ylä-Herttuala, Adam Whaley-Connell","doi":"10.1152/ajprenal.00135.2024","DOIUrl":"10.1152/ajprenal.00135.2024","url":null,"abstract":"<p><p>Diabetes mellitus is one of the leading causes of chronic kidney disease and its progression to end-stage kidney disease (ESKD). Diabetic kidney disease (DKD) is characterized by glomerular hypertrophy, hyperfiltration, inflammation, and the onset of albuminuria, together with a progressive reduction in glomerular filtration rate. This progression is further accompanied by tubulointerstitial inflammation and fibrosis. Factors such as genetic predisposition, epigenetic modifications, metabolic derangements, hemodynamic alterations, inflammation, and inappropriate renin-angiotensin-aldosterone system (RAAS) activity contribute to the onset and progression of DKD. In this context, decades of work have focused on glycemic and blood pressure reduction strategies, especially targeting the RAAS to slow disease progression. Although much of the work has focused on targeting angiotensin II, emerging data support that the mineralocorticoid receptor (MR) is integral in the development and progression of DKD. Molecular mechanisms linked to the underlying pathophysiological changes derived from MR activation include vascular endothelial and epithelial cell responses to oxidative stress and inflammation. These responses lead to alterations in the microcirculatory environment, the abnormal release of extracellular vesicles, gut dysbiosis, epithelial-mesenchymal transition, and kidney fibrosis. Herein, we present recent experimental and clinical evidence on the MR in DKD onset and progress along with new MR-based strategies for the treatment and prevention of DKD.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F519-F531"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141725275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Update: the role of epigenetics in the metabolic memory of diabetic complications. 最新进展:表观遗传学在糖尿病并发症代谢记忆中的作用。
American journal of physiology. Renal physiology Pub Date : 2024-09-01 Epub Date: 2024-07-04 DOI: 10.1152/ajprenal.00115.2024
Zhuo Chen, Vajir Malek, Rama Natarajan
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