American journal of physiology. Renal physiology最新文献

{"title":"Western diet exacerbates a murine model of Balkan nephropathy.","authors":"Yuji Oe, Young Chul Kim, Sadhana Kanoo, Helen A Goodluck, Natalia Lopez, Jolene Diedrich, Antonio Michel Pinto, K Garrett Evensen, Antonio Jose Martins Currais, Pamela Maher, Volker Vallon","doi":"10.1152/ajprenal.00185.2024","DOIUrl":"10.1152/ajprenal.00185.2024","url":null,"abstract":"<p><p>Aristolochic acid (AA) ingestion causes Balkan nephropathy, characterized by tubular injury and progression to chronic kidney disease (CKD). AA is taken up by proximal tubule cells via organic anion transport and induces p21-mediated DNA damage response, but little is known about dietary modulating factors. Western diet (WD) is rich in saturated fats and sugars and can promote metabolic disorders and CKD progression. Here, we determined the impact of WD on AA-induced kidney injury. Five-week-old male C57BL/6J mice were fed WD or normal chow (NC) for 8 wk, followed by administration of AA every 3 days for 3 wk. Measurements were performed after the last injection and following a 3-wk recovery. Independent of dosing AA by body weight (3 mg/kg/day) or same dose/mouse (0.1125 mg/day), the AA-induced increase in plasma creatinine and reduction of hematocrit were greater in WD versus NC. This was associated with increased kidney gene expression in WD vs. NC of markers of DNA damage (p21), injury (Kim1 and Ngal), and inflammation (Tnfa) and kidney fibrosis staining. WD alone increased fractional excretion of indoxyl sulfate by 7.5-fold, indicating enhanced kidney organic anion transport. Kidney proteomics identified further WD-induced changes that could increase kidney sensitivity to AA and contribute to the altered response to AA including weakening of energy metabolism, potentiation of immune and infection pathways, and disruption in RNA regulation. In conclusion, WD can increase the susceptibility of mice to Balkan nephropathy, possibly in part through facilitating kidney uptake of the organic anion AA.<b>NEW & NOTEWORTHY</b> This study shows that a Western diet (WD) aggravates a murine model of Balkan nephropathy induced by the application of the organic anion and nephrotoxin aristolochic acid (AA). Mechanistically, this may involve WD-induced kidney organic anion secretion, which can facilitate the AA uptake into proximal tubular cells and thereby contribute to the injury. Kidney proteomics identified further changes induced by feeding a WD that could have increased the sensitivity of the kidney to stress and injury.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F15-F28"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphoproteomic response to epidermal growth factor in native rat inner medullary collecting duct.","authors":"Chung-Lin Chou, Nipun U Jayatissa, Elena T Kichula, Shuo-Ming Ou, Kavee Limbutara, Mark A Knepper","doi":"10.1152/ajprenal.00182.2024","DOIUrl":"10.1152/ajprenal.00182.2024","url":null,"abstract":"<p><p>Epidermal growth factor (EGF) has important effects in the renal collecting duct to regulate salt and water transport. To identify elements of EGF-mediated signaling in the rat renal inner medullary collecting duct (IMCD), we carried out phosphoproteomic analysis. Biochemically isolated rat IMCD suspensions were treated with 1 µM of EGF or vehicle for 30 min. We performed comprehensive quantitative phosphoproteomics using tandem mass tag (TMT)-labeling of tryptic peptides followed by protein mass spectrometry. We present a data resource reporting all detected phosphorylation sites and their changes in response to EGF. For a total of 29,881 unique phosphorylation sites, 135 sites were increased and 119 sites were decreased based on stringent statistical analysis. The data are provided to users at https://esbl.nhlbi.nih.gov/Databases/EGF-phospho/. The analysis demonstrated that EGF signals through canonical EGF pathways in the renal IMCD. Analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in which EGF-regulated phosphoproteins are over-represented in native rat IMCD cells confirmed mapping to RAF-MEK-extracellular signal-regulated kinase (ERK) signaling but also pointed to a role for EGF in the regulation of protein translation. A large number of phosphoproteins regulated by EGF contained PDZ domains that are key elements of epithelial polarity determination. We also provide a collecting duct EGF-network map as a user-accessible web resource at https://esbl.nhlbi.nih.gov/Databases/EGF-network/. Overall, the phosphoproteomic data presented provide a useful resource for experimental design and modeling of signaling in the renal collecting duct.<b>NEW & NOTEWORTHY</b> EGF negatively regulates transepithelial water and salt transport across the kidney collecting duct. This study identified phosphoproteins affected by EGF stimulation in normal rat collecting ducts, providing insights into global cell signaling mechanisms. Bioinformatic analyses highlighted enhanced canonical ERK signaling alongside a diminished activity in the PI3K-Akt pathway, which is crucial for cell proliferation and survival. This EGF response differs somewhat from prior studies where both pathways were prominently activated.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F29-F47"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex differences in the adrenal circadian clock: a role for BMAL1 in the regulation of urinary aldosterone excretion and renal electrolyte balance in mice.","authors":"Hannah M Costello, Sophia A Eikenberry, Kit-Yan Cheng, Bryanna Broderick, Advay S Joshi, Gianna R Scott, Annalisse McKee, Victor M Mendez, Lauren G Douma, G Ryan Crislip, Michelle L Gumz","doi":"10.1152/ajprenal.00177.2024","DOIUrl":"10.1152/ajprenal.00177.2024","url":null,"abstract":"<p><p>Brain and muscle ARNT-Like 1 (BMAL1) is a circadian clock transcription factor that regulates physiological functions. Male adrenal-specific <i>Bmal1</i> (<i>AS^Cre/+::Bmal1</i>) KO mice displayed blunted serum corticosterone rhythms, altered blood pressure rhythm, and altered timing of eating, but there is a lack of knowledge in females. This study investigates the role of adrenal BMAL1 in renal electrolyte handling and urinary aldosterone levels in response to low salt in male and female mice. Mice were placed in metabolic cages to measure 12-h urinary aldosterone after a standard diet and 7 days low-salt diet, as well as daily body weight, 12-h food and water intake, and renal sodium and potassium balance. Adrenal glands and kidneys were collected at ZT0 or ZT12 to measure the expression of aldosterone synthesis genes and clock genes. Compared with littermate controls, <i>AS^Cre/+::Bmal1</i> KO male and female mice displayed increased urinary aldosterone in response to a low-salt diet, although mRNA expression of aldosterone synthesis genes was decreased. Timing of food intake was altered in <i>AS^Cre/+::Bmal1</i> KO male and female mice, with a blunted night/day ratio. <i>AS^Cre/+::Bmal1</i> KO female mice displayed decreases in renal sodium excretion in response to low salt, but both male and female KO mice had changes in sodium balance that were time-of-day-dependent. In addition, sex differences were found in adrenal and kidney clock gene expression. Notably, this study highlights sex differences in clock gene expression that could contribute to sex differences in physiological functions.<b>NEW & NOTEWORTHY</b> Our findings highlight the importance of sex as well as time-of-day in understanding the role of the circadian clock in the regulation of homeostasis. Time-of-day is a key biological variable that is often ignored in research, particularly in preclinical rodent studies. Our findings demonstrate important differences in several measures at 6 AM compared with 6 PM. Consideration of time-of-day is critical for the translation of findings in nocturnal rodent physiology to diurnal human physiology.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F1-F14"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal Barrier Function Declines During Polycystic Kidney Disease Progression.","authors":"Randee Sedaka, Caleb Lovelady, Emily Hallit, Branden Duyvestyn, Sejal Shinde, Aida Moran-Reyna, Goo Lee, Shinobu Yamaguchi, Craig L Maynard, Takamitsu Saigusa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Most patients with autosomal dominant polycystic kidney disease (ADPKD) develop kidney cysts due to germline <i>PKD1</i> mutations. In the kidney, <i>Pkd1</i> loss impairs epithelial cell integrity and increases macrophage infiltration, contributing to cyst growth. Despite its role as the body's largest inflammatory cell reservoir, it has yet to be elucidated whether a similar phenotype presents in the intestines. We hypothesize that loss of <i>Pkd1</i> leads to a leaky intestinal epithelial barrier and increased inflammation, prior to rapid cystogenesis. Control and inducible, global <i>Pkd1</i> knockout (<i>Pkd1</i>KO) mice were euthanized at 3 and 6 months of age (early and late stage) to evaluate kidney disease progression, small and large intestinal integrity, and inflammation. Early-stage <i>Pkd1</i>KO mice displayed mild cystic kidneys and tubular injury with preserved kidney function. Intestinal epithelial barrier was tighter in KO mice, which was associated with higher expression of cell-cell epithelial integrity markers. However, there was no evidence of local or systemic inflammation in either genotype. Late-stage <i>Pkd1</i>KO mice had severely cystic, impaired kidneys with increased expression of integrity markers, tubular injury and inflammation. Intestinal epithelial barrier was leakier in late stage <i>Pkd1</i>KO mice, accompanied by gene reduction of integrity markers, increased inflammation and elevated water and sodium channel expression. Gut motility and fecal water excretion were increased in <i>Pkd1</i>KO compared to flox mice irrespective of age. Overall, kidney injury appears to precede intestinal injury in ADPKD, whereby the intestinal barrier becomes leaky as cystogenesis progresses.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"None"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remote organ cancer induces kidney injury, inflammation, and fibrosis and adversely alters renal function.","authors":"Dana Hammouri, Andrew Orwick, Mark A Doll, Dianet Sanchez Vega, Parag P Shah, Christopher J Clarke, Brian Clem, Levi J Beverly, Leah J Siskind","doi":"","DOIUrl":"","url":null,"abstract":"<p><p><b>Background:</b> Approximately 30% of cancer patients experience kidney complications, which hinder optimal cancer management, imposing a burden on patients' quality of life and the healthcare system. The etiology of kidney complications in cancer patients is often attributed to oncological therapies. However, the direct impact of cancer on kidney health is underestimated. Our previous study demonstrated that metastatic lung cancer adversely alters the kidney, and exacerbates chemotherapy-induced nephrotoxicity, indicating lung cancer-kidney crosstalk. The current study examines whether this phenomenon is specific to the employed cancer model. <b>Methods:</b> Female and male mice of various strains were injected with different cell lines of remote organ cancer and their kidney tissues were analyzed for toxicity and fibrosis. <b>Results:</b> The impact of cancer on the kidney varied by cancer type. Breast cancer and specific subtypes of lung cancer, including KRAS- and EGFR-mutant cancer, pathologically altered kidney physiology and function in a manner dependent on the metastatic potential of the cell line. This was independent of mouse strain, sex, and cancer cell line origin. Moreover, tumor DNA was not detected in the renal tissue, excluding metastases to the kidney as a causative factor for the observed pathological alterations. Lewis lung carcinoma and B16 melanoma did not cause nephrotoxicity, regardless of the tumor size. <b>Conclusion:</b> Our results confirm cancer-kidney crosstalk in specific cancer types. In the era of precision medicine, further research is essential to identify at-risk oncology populations, enabling early detection and management of renal complications.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"None"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from TREM-2 knocked-out macrophages alleviated renal fibrosis via HSPa1b/AKT pathway.","authors":"Zihao Xiao, Yajie Wang, Yuye Chen, Ling Jin, Yuanhui Shi, Can Liu, Cong Fu, Yuhan Cao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Macrophages are recognized as vital players in renal fibrosis, with a high degree of heterogeneity and plasticity, and the triggering receptor expressed on myeloid cell-2 (TREM-2) is highly expressed on macrophages and participates in the progression of tissue fibrosis. However, the mechanism by which TREM-2 mediates the progression of renal fibrosis is still unclear. Our study revealed that exosomes derived from TREM-2-deficient (TREM-2^-/-) macrophages suppressed the progression of fibrosis, as indicated by a greater matrix metalloproteinase-9 (MMP-9)/tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) ratio at the protein level in secreted exosomes than in exosomes from wild-type (WT) macrophages in the fibrotic microenvironment. In addition, renal tubular epithelial cells (TECs) engulfed these nanoscale vesicles, and the expression of collagen I and α-smooth muscle actin (α-SMA) (a fibrosis-related marker) was obviously decreased. Through RNA-seq, we found that TREM-2^-/- macrophages increase the MMP-9/TIMP-1 ratio in their exosomes via the HSPa1b/AKT pathway. Notably, renal fibrosis was effectively alleviated in the obstructed kidneys of mice that received a renal pelvis injection of an adeno-associated virus (AAV-shTREM-2) containing the sequence used to silence TREM-2. However, VER-155008 (an inhibitor of HSPa1b) and Ly294002 (an inhibitor of AKT) reversed this effect. Moreover, polyclonal antibodies against TREM-2 also effectively relieved UUO-induced renal fibrosis. Overall, we validated that knocking down TREM-2 expression can inhibit the progression of renal fibrosis through a macrophage exosome-dependent pathway both in vitro and in vivo. Hence, our findings suggest that TREM-2 is a potential therapeutic target for CKD.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"None"},"PeriodicalIF":0.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of branched chain amino acid catabolism protects against nephrotoxic acute kidney injury.","authors":"Samaneh DiMartino, Monica P Revelo, Sandeep K Mallipattu, Sian E Piret","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a major risk factor for chronic kidney disease (CKD), and there are currently no therapies for AKI. Proximal tubules (PT) are particularly susceptible to AKI, including due to nephrotoxins such as aristolochic acid I (AAI). Normal PT utilize fatty acid oxidation and branched chain amino acid (BCAA; valine, leucine, isoleucine) catabolism to generate ATP; however, in AKI, these pathways are downregulated. Our aim was to investigate the utility of a pharmacological activator of BCAA catabolism, BT2, in preventing nephrotoxic AKI. Mice were administered two injections of AAI 3 days apart to induce AKI, with or without daily BT2 treatment. Mice treated with BT2 had significantly protected kidney function (reduced serum creatinine and urea nitrogen), reduced histological injury, preservation of PT (Lotus lectin staining), and less PT injury (cytokeratin-20 staining) and inflammatory gene expression compared to mice with AAI alone. Mice with AKI had increased circulating BCAA and accumulation of BCAA in the kidney cortex. Leucine is a potent activator of mechanistic target of rapamycin complex 1 (mTORC1) signaling, and mTORC1 signaling was activated in mice treated with AAI. However, BT2 reduced kidney cortical BCAA accumulation, and attenuated the mTORC1 signaling. <i>In vitro</i>, injured primary PT cells had compromised mitochondrial bioenergetics, but cells treated with AAI+BT2 had partially restored mitochondrial bioenergetics, and improved injury markers compared to cells treated with AAI alone. Thus, pharmacological activation of BCAA catabolism using BT2 attenuated nephrotoxic AKI in mice.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"None"},"PeriodicalIF":0.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142803847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pax proteins mediate segment-specific functions in proximal tubule survival and response to ischemic injury.","authors":"Madison C McElliott, Asha C Telang, Jenna T Ference-Salo, Anas Al-Suraimi, Mahboob Chowdhury, Edgar A Otto, Abdul Soofi, Gregory R Dressler, Jeffrey A Beamish","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a common clinical syndrome with few effective treatments. Though the kidney can regenerate after injury, the molecular mechanisms regulating this process remain poorly understood. Pax2 and Pax8 are DNA-binding transcription factors that are upregulated after kidney injury. However, their function during the response to AKI remains incompletely defined. In this report, we develop a model of ischemic AKI in female mice with mosaic nephrons comprised of both Pax2 and Pax8 mutant and wildtype proximal tubule cells with fixed lineages. Each population therefore experiences identical physiological and injury conditions in the same animal. In these female mice, we show that before injury the S1 and S2 segments of the proximal tubule are depleted of Pax-mutant cells while mutant cells are preserved in the S3 segment. Retained S3 Pax-mutant cells develop a preconditioned phenotype that overlaps with gene expression signatures in AKI. In response to ischemic AKI, which most strongly damages the S3 proximal tubule, injury-resistant mutant S3 cells are more likely to proliferate. Pax-mutant cells then preferentially repopulate the S3 segment of the proximal tubule. Our results indicate that Pax2 and Pax8 are not required for regeneration of the S3 proximal tubule after ischemic AKI. Together, our findings indicate that Pax proteins play a critical role determining the segment-specific proximal tubule gene expression patterns that dictate vulnerability to ischemic injury.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"None"},"PeriodicalIF":0.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deletion of AT_1a receptors selectively in the proximal tubules of the kidney alters the hypotensive and natriuretic response to atrial natriuretic peptide via NPR_A/cGMP/NO signaling.","authors":"Xiao Chun Li, Chih-Hong Wang, Rumana Hassan, Akemi Katsurada, Ryosuke Sato, Jia Long Zhuo","doi":"10.1152/ajprenal.00160.2024","DOIUrl":"10.1152/ajprenal.00160.2024","url":null,"abstract":"&lt;p&gt;&lt;p&gt;In the proximal tubules of the kidney, angiotensin II (ANG II) binds and activates ANG II type 1 (AT&lt;sub&gt;1a&lt;/sub&gt;) receptors to stimulate proximal tubule Na&lt;sup&gt;+&lt;/sup&gt; reabsorption, whereas atrial natriuretic peptide (ANP) binds and activates natriuretic peptide receptors (NPR&lt;sub&gt;A&lt;/sub&gt;) to inhibit ANG II-induced proximal tubule Na&lt;sup&gt;+&lt;/sup&gt; reabsorption. These two vasoactive systems play important counteracting roles to control Na&lt;sup&gt;+&lt;/sup&gt; reabsorption in the proximal tubules and help maintain blood pressure homeostasis. However, how AT&lt;sub&gt;1a&lt;/sub&gt; and NPR&lt;sub&gt;A&lt;/sub&gt; receptors interact in the proximal tubules and whether natriuretic effects of NPR&lt;sub&gt;A&lt;/sub&gt; receptor activation by ANP may be potentiated by deletion of AT&lt;sub&gt;1&lt;/sub&gt; (AT&lt;sub&gt;1a&lt;/sub&gt;) receptors selectively in the proximal tubules have not been studied previously. The present study used a novel mouse model with proximal tubule-specific knockout of AT&lt;sub&gt;1a&lt;/sub&gt; receptors, PT-&lt;i&gt;Agtr1a&lt;/i&gt;&lt;sup&gt;-/-&lt;/sup&gt;, to test the hypothesis that deletion of AT&lt;sub&gt;1a&lt;/sub&gt; receptors selectively in the proximal tubules augments the hypotensive and natriuretic responses to ANP. Basal blood pressure was about 16 ± 3 mmHg lower (&lt;i&gt;P&lt;/i&gt; &lt; 0.01), fractional proximal tubule Na&lt;sup&gt;+&lt;/sup&gt; reabsorption was significantly lower (&lt;i&gt;P&lt;/i&gt; &lt; 0.05), whereas 24-h urinary Na&lt;sup&gt;+&lt;/sup&gt; excretion was significantly higher, in PT-&lt;i&gt;Agtr1a&lt;/i&gt;&lt;sup&gt;-/-&lt;/sup&gt; mice than in wild-type mice (&lt;i&gt;P&lt;/i&gt; &lt; 0.01). Infusion of ANP via osmotic minipump for 2 wk (0.5 mg/kg/day ip) further significantly decreased blood pressure and increased the natriuretic response in PT-&lt;i&gt;Agtr1a&lt;/i&gt;&lt;sup&gt;-/-&lt;/sup&gt; mice by inhibiting proximal tubule Na&lt;sup&gt;+&lt;/sup&gt; reabsorption compared with wild-type mice (&lt;i&gt;P&lt;/i&gt; &lt; 0.01). These augmented hypotensive and natriuretic responses to ANP in PT-&lt;i&gt;Agtr1a&lt;/i&gt;&lt;sup&gt;-/-&lt;/sup&gt; mice were associated with increased plasma and kidney cGMP levels (&lt;i&gt;P&lt;/i&gt; &lt; 0.01), kidney cortical NPR&lt;sub&gt;A&lt;/sub&gt; and NPR&lt;sub&gt;C&lt;/sub&gt; mRNA expression (&lt;i&gt;P&lt;/i&gt; &lt; 0.05), endothelial nitric oxide (NO) synthase (eNOS) and phosphorylated eNOS proteins (&lt;i&gt;P&lt;/i&gt; &lt; 0.01), and urinary NO excretion (&lt;i&gt;P&lt;/i&gt; &lt; 0.01). Taken together, the results of the present study provide further evidence for important physiological roles of intratubular ANG II/AT&lt;sub&gt;1a&lt;/sub&gt; and ANP/NPR&lt;sub&gt;A&lt;/sub&gt; signaling pathways in the proximal tubules to regulate proximal tubule Na&lt;sup&gt;+&lt;/sup&gt; reabsorption and maintain blood pressure homeostasis.&lt;b&gt;NEW & NOTEWORTHY&lt;/b&gt; This study used a mutant mouse model with proximal tubule-selective deletion of angiotensin II (ANG II) type 1 (AT&lt;sub&gt;1a&lt;/sub&gt;) receptors to study, for the first time, important interactions between ANG II/AT&lt;sub&gt;1&lt;/sub&gt; (AT&lt;sub&gt;1a&lt;/sub&gt;) receptor/Na&lt;sup&gt;+&lt;/sup&gt;/H&lt;sup&gt;+&lt;/sup&gt; exchanger 3 and atrial natriuretic peptide (ANP)/natriuretic peptide receptor (NPR&lt;sub&gt;A&lt;/sub&gt;)/cGMP/nitric oxide signaling pathways in the proximal tubules. The results of the present study provide f","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F946-F956"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenesis of HIV-associated nephropathy in children and adolescents: taking a hard look 40 years later in the era of gene-environment interactions.","authors":"Patricio E Ray, Jinliang Li, Jharna Das, Lian Xu, Jing Yu, Zhe Han","doi":"10.1152/ajprenal.00208.2024","DOIUrl":"10.1152/ajprenal.00208.2024","url":null,"abstract":"<p><p>HIV-associated nephropathy (HIVAN) is a kidney disease that affects mainly people of African ancestry with a high HIV-1 viral load. New antiretroviral therapies (ART) have been highly efficient in preventing and improving the outcome of HIVAN. However, providing chronic ART to children and adolescents living with HIV (CALWH) remains a significant challenge all over the world. More than 2.5 million CALWH, including those living in Sub-Saharan Africa, continue to be at high risk of developing HIVAN. Much of our understanding of the pathogenesis of HIVAN is based on studies conducted in transgenic mice and adults with HIVAN. However, CALWH may experience different health outcomes, risk factors, and susceptibilities to HIVAN in comparison to adults. This article reviews the progress made over the last 40 years in understanding the pathogenesis of HIVAN in CALWH, focusing on how the HIV virus, alongside genetic and environmental factors, contributes to the development of this disease. The landmark discovery that two risk alleles of the apolipoprotein-1 (APOL1) gene play a critical role in HIVAN has significantly advanced our understanding of the disease's pathogenesis. However, we still need to understand why renal inflammation persists despite ART and determine whether the kidney may harbor HIV reservoirs that need to be eliminated to cure HIV permanently. For these reasons, we emphasize reviewing how HIV-1 infects renal cells, affects their growth and regeneration, and discussing how inflammatory cytokines and APOL1 affect the outcome of childhood HIVAN.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":"F1049-F1066"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142334171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}