British Poultry Science最新文献_第9页

Properties of encapsulated raspberry powder and its efficacy for improving the pH, colour quality and shelf life of modified atmosphere packaged chicken nuggets. 封装覆盆子粉的特性及其对改善改良气调包装鸡块的 pH 值、色泽质量和货架期的功效。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-10-01 Epub Date: 2024-07-12 DOI: 10.1080/00071668.2024.2359988

M I Aksu, H Arslan

{"title":"Properties of encapsulated raspberry powder and its efficacy for improving the pH, colour quality and shelf life of modified atmosphere packaged chicken nuggets.","authors":"M I Aksu, H Arslan","doi":"10.1080/00071668.2024.2359988","DOIUrl":"10.1080/00071668.2024.2359988","url":null,"abstract":"1. Recent research has shown that encapsulated raspberry powder (RP) is a natural colourant for foodstuffs. However, no research has been conducted on its use in chicken nuggets. In addition, the effect of RP on products with and without phosphate addition is unknown. This study assessed the effects of RP (control, 0.5%, 1.0%) and phosphate (0.0%, 0.3%) on the pH and colour quality properties of nuggets.2. In the production of RP, red raspberry (Rubus ideaus L.) juices were encapsulated using maltodextrin in a spray-dryer. Antioxidant activity, total anthocyanin, total phenolics, colour, moisture and pH analyses of the RP were performed.3. Nuggets were packaged in modified atmosphere packaging (MAP; 40%CO2 + 60%N2) and were stored at 2.0 ± 0.5°C for 120 d. The pH and external and internal surface colour (L*, a*, b*, C* and h) values were measured on d 0, 15, 30, 45, 60, 75, 90, 105 and 120 of storage.4. The addition of phosphate increased the pH in the samples, while these decreased with the addition of RP (p < 0.05). During storage, the highest pH were seen in the phosphate samples and the lowest in the nuggets with 1.0% RP addition (p < 0.05).5. With the addition of phosphate, the external surface a* value of nuggets increased (p < 0.05). Depending on the level of RP added to the nuggets, the external surface L* value decreased and a* and b* values increased (p < 0.05). After d 30 of storage, the a* value increased in the samples with RP addition and this increase was higher in the with phosphate nuggets (p < 0.05).6. The internal surface a* value increased with the addition of RP during nugget production (p < 0.05). The increase in a* value was greater in samples with added phosphate (p < 0.05). During storage, the highest a* values were seen in nuggets treated with phosphate + 0.1% RP (p < 0.05). The addition of RP to chicken nugget emulsion improved redness, colour stability and shelf life.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"559-573"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of limestone particle size and microbial phytase on phosphorus and calcium digestion kinetics along the gastrointestinal tract in laying hens. 石灰石粒度和微生物植酸酶对蛋鸡胃肠道磷和钙消化动力学的影响

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-10-01 Epub Date: 2024-05-29 DOI: 10.1080/00071668.2024.2357659

F Hervo, M-P Létourneau-Montminy, B Méda, A Narcy

{"title":"Effect of limestone particle size and microbial phytase on phosphorus and calcium digestion kinetics along the gastrointestinal tract in laying hens.","authors":"F Hervo, M-P Létourneau-Montminy, B Méda, A Narcy","doi":"10.1080/00071668.2024.2357659","DOIUrl":"10.1080/00071668.2024.2357659","url":null,"abstract":"1. This study assessed the effect of limestone particle size and microbial phytase incorporation on the fate of phosphorus (P) and calcium (Ca) along the gastrointestinal tract in 72 laying hens.2. Four experimental diets were formulated according to a 2 × 2 factorial arrangement to evaluate the effect of two coarse limestone (CL) inclusion. This included a mix (MIX) of 75% CL (2 - 4 mm) and 25% fine particles (FL, <0.5 mm) or 100% FL, in two different basal diets formulated without (MIX0 and FL0) or with 300 FTU of microbial phytase/kg (MIX300 and FL300).3. Contents of the crop, gizzard, duodenum, jejunum and ileum were collected to determine the mean retention time (MRT) of dry matter (DM), the recovery rate of Ca and P in each segment of the gastrointestinal tract and the apparent fractional digestibility coefficient (AD) of Ca and P in each intestinal segment.4. In hens fed FL, microbial phytase decreased the MRT of DM along the intestine (p < 0.05). In the crop and the gizzard, Ca recovery increased with MIX incorporation to a greater extent in hens fed without microbial phytase (p < 0.05). The mixed particle size incorporation decreased absorption kinetics of Ca in hens fed microbial phytase. The AD of P and the absorption kinetics of P were significantly decreased in hens receiving FL300, probably due to complex formation between Ca and phytic acid.5. This study showed that coarse limestone particles incorporation improved mineral utilisation along the digestive tract.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"605-614"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141160780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Food safety control in poultry industry: prevalence and antimicrobial susceptibility of Escherichia coli isolated from raw chicken and the potential use of Origanum essential oils as alternative to antibiotics. 家禽业的食品安全控制：从生鸡肉中分离出的大肠埃希氏菌的流行率和抗菌药敏感性，以及牛至精油作为抗生素替代品的潜在用途。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-07-12 DOI: 10.1080/00071668.2024.2346307

C Abou-Jaoudeh, J Khalil, E El-Hayek, R Abou-Khalil

{"title":"Food safety control in poultry industry: prevalence and antimicrobial susceptibility of Escherichia coli isolated from raw chicken and the potential use of Origanum essential oils as alternative to antibiotics.","authors":"C Abou-Jaoudeh, J Khalil, E El-Hayek, R Abou-Khalil","doi":"10.1080/00071668.2024.2346307","DOIUrl":"10.1080/00071668.2024.2346307","url":null,"abstract":"1. The extensive use of antimicrobials in poultry production may contribute to the emergence of resistant bacteria. This study was conducted to determine the prevalence and resistance of different E. coli strains isolated from raw chicken meat and to investigate the possibility to use Lebanese native oregano essential oils as alternatives.2. In total, 250 chickens from Lebanese markets were examined for the presence of E. coli. Isolates were then screened for susceptibility using 19 antibiotics and two essential oils extracted from oregano plants.3. Of the 250 chickens tested, 80% were contaminated with E. coli. Main resistance was seen against amoxycillin, ampicillin, penicillin, tetracycline, tylosin, streptomycin and erythromycin. The highest rate of sensitivity was found in 86.1% of strains to Amoxycillin/Clavulanic acid, 80.09% to Tilmicosin. Both essential oils from Origanum syriacum (98%) and O. ehrenbergii (97.3%) showed promising potential in inhibiting the growth of the tested bacteria. Oil from O. syriacum exhibited superior efficacy against 200 E. coli strains, inhibiting 46.1% at 200 mg/l and all at 400 mg/l, while O. ehrenbergii oil showed slightly lower inhibition, affecting 41.6% at 200 mg/l and all at 400 mg/l.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"494-501"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimbiotic and wheat bran inclusion in maize or wheat-based diets for broiler chickens; effects on jejunal histomorphology, digesta oligosaccharides and caecal short-chain fatty-acids profiles. 在以玉米或小麦为基础的肉鸡日粮中添加刺激性益生菌和麦麸；对空肠组织形态学、消化道低聚糖和盲肠短链脂肪酸谱的影响。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-07-12 DOI: 10.1080/00071668.2024.2338535

S Veluri, G González-Ortiz, M R Bedford, O A Olukosi

{"title":"Stimbiotic and wheat bran inclusion in maize or wheat-based diets for broiler chickens; effects on jejunal histomorphology, digesta oligosaccharides and caecal short-chain fatty-acids profiles.","authors":"S Veluri, G González-Ortiz, M R Bedford, O A Olukosi","doi":"10.1080/00071668.2024.2338535","DOIUrl":"10.1080/00071668.2024.2338535","url":null,"abstract":"1. A stimbiotic (STB) is any feed additive that stimulates caeca fibre fermentation, although the additive itself contributes little to the caeca short-chain fatty acid (SCFA) production. A 42 d experiment investigated the interactive effects of STB and wheat bran (WB) in broiler chickens receiving maize or wheat-based diets.2. The treatments were arranged in a 2 × 2 × 2 factorial (eight replicates each), the dietary factors being diet (maize-SBM or wheat-SBM), STB (with or without) and WB (0 or 50 g/kg). Jejunal tissue, gizzard, jejunal and ileal digesta and caecal contents were collected on d 18 and 42.3. Gizzard pH tended to decrease with STB (p = 0.06) supplementation and was lower in birds fed wheat- compared to maize-based diets on d 18 (p < 0.05). Birds receiving diets with WB had higher jejunum pH on d 18 (p < 0.05).4. Total short-chain fatty acids (SCFA) in the caeca on d 18 and isobutyrate on d 42 were higher (p < 0.05) for maize compared with wheat-based diets. However, on d 42, acetate, butyrate and total SCFA were higher (p < 0.05) for wheat-based compared with maize-based diets.5. On d 18, STB and WB inclusion increased villi height (VH; p < 0.05) and VH to crypt depth ratio (VH/CD), respectively (p < 0.05). On d 42, VH (p < 0.05) and VH/CD were higher in wheat-based diets (p < 0.05). The VH/CD ratio was lower with STB supplementation (p < 0.05). Marker-corrected pentose oligosaccharides (Pent)4 and (Pent)5 concentrations in the ileal digesta were reduced (p < 0.05) with STB supplementation. In addition, STB decreased (Pent)3 concentration in maize-, but not wheat-based diets (p < 0.05).6. In conclusion, both WB and STB influenced gastrointestinal pH and jejunum histomorphology of broilers without increasing oligosaccharide concentration in the ileum and SCFA in the caeca.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"465-477"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New primers for sex identification in the Chukar partridges (Alectoris chukar). 用于鉴定楚卡鹧鸪（Alectoris chukar）性别的新引物。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1080/00071668.2024.2332723

X X Jia, W Q Chen, X J Tang, J X Lu, Y F Fan, Y S Gao

引用次数: 0

Genetic analysis of a Kaijiang duck conservation population through genome-wide scan. 通过全基因组扫描对开江鸭保护种群进行遗传分析。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1080/00071668.2024.2335937

Z Shen, T Zhang, G Twumasi, J Zhang, J Wang, Y Xi, R Wang, J Wang, R Zhang, H Liu

{"title":"Genetic analysis of a Kaijiang duck conservation population through genome-wide scan.","authors":"Z Shen, T Zhang, G Twumasi, J Zhang, J Wang, Y Xi, R Wang, J Wang, R Zhang, H Liu","doi":"10.1080/00071668.2024.2335937","DOIUrl":"10.1080/00071668.2024.2335937","url":null,"abstract":"1. The Kaijiang duck is a native Chinese breed known for its excellent egg laying performance, killing-out percentage (88.57%), and disease resistance. The assessment of population genetic structure is the basis for understanding the genetics of indigenous breeds and for their protection and management.2. In this study, whole-genome sequencing was performed on 60 Kaijiang ducks to identify genetic variations and investigate the population structure. Homozygosity (ROH) analysis was conducted to assess inbreeding levels in the population.3. The study revealed a moderate level of inbreeding, indicated by an average inbreeding coefficient of 0.1043. This may impact the overall genetic diversity.4. Genomic Regions of Interest identified included 168 genomic regions exhibiting high levels of autozygosity. These regions were associated with processes including muscle growth, pigmentation, neuromodulation, and growth and reproduction.5. The significance of these pathways indicated their potential role in shaping the desirable traits of the Kaijiang duck. These findings provide insights into the genetic basis of the Kaijiang duck's desirable traits and can inform future breeding and conservation efforts.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"378-386"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of circRNA expression profiles associated with non-Mendelian inheritance in feather growth of chickens. 与鸡羽毛生长的非孟德尔遗传相关的 circRNA 表达谱特征。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1080/00071668.2024.2339485

M Qiu, C Yu, S Zhu, C Hu, L Yang, X Song, B Xia, X Jiang, H Du, Q Li, Z Zhang, C Yang

{"title":"Characterization of circRNA expression profiles associated with non-Mendelian inheritance in feather growth of chickens.","authors":"M Qiu, C Yu, S Zhu, C Hu, L Yang, X Song, B Xia, X Jiang, H Du, Q Li, Z Zhang, C Yang","doi":"10.1080/00071668.2024.2339485","DOIUrl":"10.1080/00071668.2024.2339485","url":null,"abstract":"1. Non-coding RNAs, such as miRNAs, play a crucial role in chicken feather growth rate. However, circular RNA (circRNA) expression profiles in fast- and slow-feathering chickens that follow and do not follow Mendelian inheritance are unclear.2. The circRNA expression profiles was analysed by RNA sequencing of hair follicles of slow-feathering chickens that follow genetic rules and fast-feathering chickens that did not follow genetic rules. Differentially expressed circRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network was then constructed and the key factors and regulation mechanisms controlling feather growth rate were identified.3. The results revealed that 67 circRNAs were significantly differentially expressed in hens, including 22 up-regulated and 45 down-regulated circRNAs in non-Mendelian inheritance-mediated fast-feathering hens compared with Mendelian inheritance-mediated slow-feathering hens. In addition, 16 significantly differentially expressed circRNAs were identified in cockerels, including nine up-regulated and seven down-regulated circRNAs in non-Mendelian inheritance-mediated fast- compared with Mendelian inheritance-mediated slow-feathering cocks. Moreover, circRNA-mediated ceRNA regulation of hair follicle formation was particularly abundant in the Jak-STAT, Wnt and Toll-like receptor signalling pathways. Furthermore, circABI3BP was seen to be a crucial circRNA in regulating feather growth rate, by binding with gga-miR-1649-5p to regulate SSTR2 expression.4. In conclusion, this study analysed circRNA expression profiles in fast- and slow-feathering chickens that follow and do not follow Mendelian inheritance, which laid the foundation for understanding the role of circRNA in chicken feather growth rate.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"371-377"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin ameliorates lipid deposition in primary hepatocytes of the chicken embryo. 槲皮素可改善鸡胚胎原代肝细胞中的脂质沉积。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-05-10 DOI: 10.1080/00071668.2024.2332717

Y Feng, C Zhao, T Li, M Wang, B R Serrano, A R Barcenas, L Qu, W Zhao, M Shen

{"title":"Quercetin ameliorates lipid deposition in primary hepatocytes of the chicken embryo.","authors":"Y Feng, C Zhao, T Li, M Wang, B R Serrano, A R Barcenas, L Qu, W Zhao, M Shen","doi":"10.1080/00071668.2024.2332717","DOIUrl":"10.1080/00071668.2024.2332717","url":null,"abstract":"1. The accumulation of excessive fat plays a role in the development of non-alcoholic fatty liver disease (NAFLD) and phytogenic feed additives have the potential to ameliorate this. This study involved the isolation and culture of primary hepatocytes from chicken embryos to establish a model of hepatic steatosis induced by oleic acid/dexamethasone (OA/DEX). Lipid accumulation and cell viability were assessed using Nile Red staining, Oil Red O staining and cell count Kit -8 (CCK8) following treatment with varying concentrations of quercetin (Que). The potential mechanism by which Que exerts its effects was preliminarily investigated.2. The results indicated that OA effectively treated lipid accumulation in hepatocytes. There was no notable variance in cell proliferation between the normal and OA/DEX groups when subjected to Que treatment at concentrations of 1000 ng/ml and 10 000 ng/ml. Triglycerides and cholesterol (low and high density) decreased with Que treatment, with the most substantial reduction observed at 10 000 ng/ml.3. Gene expression levels decreased to levels similar to those in the control groups. Western blot data demonstrated that sterol regulatory element-binding protein 1 (SREBP-1) protein expression correlated with its mRNA expression level. Que mitigated lipid accumulation through the alpha serine/threonine protein kinase (AKT) and extracellular signal-regulated kinase (ERK) pathways. Expression levels of lipid-related genes (APOB, PPARα, CYP3A5 and SREBP-1) decreased to levels similar to the control groups. Western blot data demonstrated that the SREBP-1 protein expression correlated with its mRNA expression level.4. Supplementation with Que ameliorated lipid accumulation through AKT and ERK signalling pathway in OA/DEX-induced high-fat hepatocytes.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"429-436"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative transcriptomic analyses reveal key genes underlying melanin distribution during embryonic development in geese (Anser anser). 比较转录组分析揭示了鹅（Anser anser）胚胎发育过程中黑色素分布的关键基因。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1080/00071668.2024.2335943

Y Yang, C Wang, Y Liu, G Li, X Wang, H Wang, D He

{"title":"Comparative transcriptomic analyses reveal key genes underlying melanin distribution during embryonic development in geese (Anser anser).","authors":"Y Yang, C Wang, Y Liu, G Li, X Wang, H Wang, D He","doi":"10.1080/00071668.2024.2335943","DOIUrl":"10.1080/00071668.2024.2335943","url":null,"abstract":"1. Melanin distribution typically exhibits a gradient dilution along the dorsal-ventral axis of the body, including in domestic geese. However, the specific genes and molecular mechanisms responsible for this melanin distribution pattern remain incompletely understood.2. The transcriptomic comparisons were conducted at three embryonic stages, specifically on embryonic d 15 (E15), 22 (E22), and 29 (E29), between the pigmented dorsal skin and the depigmented distal foot.3. Differentially expressed genes (DEGs) associated with melanin synthesis were identified, particularly TYR, TYRP1, and EDNRB2, which exhibited significantly higher expression levels in the dorsal skin at E15 and E22. However, expression levels significantly decreased in later stages (E29).4. The ASIP gene showed remarkably high-expression levels in the distal feet compared to the dorsal skin post-E22 stage (log2FC: 5.31/6.88 at E22/E29). Gene Ontology (GO) enrichment analysis detected eight terms associated with melanin synthesis and melanosome formation (p < 0.05), including melanosome membrane (GO: 0033162) and melanin biosynthetic process (GO: 0042438). Additionally, KEGG pathway analysis showed significant enrichment of the melanogenesis pathway (hsa004916) at d 22 (E22).","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"387-393"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of genes related to ileal barrier function in heritage and modern broiler chickens. 传统和现代肉鸡回肠屏障功能相关基因的表达。

IF 1.6 3区农林科学

British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1080/00071668.2024.2337187

K R Kpodo, K B Miska, L L Schreier, M Proszkowiec-Weglarz

{"title":"Expression of genes related to ileal barrier function in heritage and modern broiler chickens.","authors":"K R Kpodo, K B Miska, L L Schreier, M Proszkowiec-Weglarz","doi":"10.1080/00071668.2024.2337187","DOIUrl":"10.1080/00071668.2024.2337187","url":null,"abstract":"1. An experiment was conducted to determine differences in the expression of genes encoding intestinal barrier proteins between fast, medium and slow-growing chickens. Chicken breeds Athens Canadian Random Bred (ACRB), Longenecker's Heritage (LHR), RedBro, Hubbard H1 (HH1), Cobb500 and Ross708 were raised from hatch for 35 d.2. Ileal samples were collected at embryonic day E19 (-2 days post-hatch), hatch and d 7, 14, 21, 28 and 35 post-hatch to assess the expression of genes encoding tight junction proteins (claudins, CLDN; occludin, OCLN; zonula occludens, ZO; and junctional adhesion molecules, JAM), mucin (Muc2), immunoglobulin A (IgA), polymeric immunoglobulin receptor (pIgR) and fatty acid binding protein (FABP2).3. Expression of CLDN-1 was increased (p < 0.0001) in LHR compared to Cobb500 while CLDN-5 was increased (p < 0.0001) in ACRB, HH1, RedBro and Ross708 compared to LHR as well as in ACRB compared to Cob500. Occludin was upregulated (p = 0.01) in ACRB and LHR compared to Ross708 at d 14 post-hatch. Expression of ZO-1 was upregulated (p = 0.001) in LHR compared to Ross708, HH1 and Cobb500. Tight junction genes, except CLDN-4, JAM-2 and JAM-3 were downregulated (p < 0.0001) at hatch and d 7 post-hatch. Expression of Muc2 was increased (p < 0.0001) in LHR compared to RedBro and from -2 d to d 7 post-hatch.4. Immunoglobulin A was increased (p = 0.001) in LHR compared to Ross708 and HH1 at -2 d post-hatch and in LHR compared to ACRB, Cobb500 and Ross708 at hatch. In addition, IgA expression was increased in all breeds at d 14 post-hatch while pIgR was upregulated (p = 0.02) in Cobb500 and Ross708 compared to ACRB, HH1, LHR and RedBro at hatch.5. The gene expression patterns suggest that selection for growth may have not induced changes in junctional complexes and immune defence genes. However, the results confirmed that the expression of these genes is age dependent.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"437-447"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0