British Poultry Science最新文献

{"title":"Comprehensive estimation of overfeeding influence on goose meat quality.","authors":"R Wei, C Han","doi":"10.1080/00071668.2025.2455520","DOIUrl":"10.1080/00071668.2025.2455520","url":null,"abstract":"<p><p>1. The aim of this research was to explore the influence of overfeeding on goose meat quality in <i>foie gras</i> production. Forty Tianfu Meat Geese were averagely separated into normal-feeding group (control group) and overfeeding group (force-feeding group), randomly. After overfeeding, the breast muscle and leg muscle were collected, and then the determinations of meat quality variables were performed. The cluster analysis, principal component analysis (PCA) and partial least square-discriminate analysis (PL-SDA) were performed to comprehensively estimate the influence of overfeeding on goose meat quality.2. Overfeeding increased the weights of breast muscle and leg muscle (<i>p <</i> 0.05), increased L*, a* and b* values of breast muscle and leg muscle (<i>p <</i> 0.05), increased the hardness values of breast muscle and leg muscle (<i>p <</i> 0.05), decreased the cooking loss of breast muscle (<i>p <</i> 0.05). In nutritional variables, overfeeding increased the contents of crude fat of breast muscle and leg muscle (<i>p <</i> 0.05). In breast muscle, overfeeding increased the contents of Ala, Tyr, Lys and Val, and decreased the contents of Arg and Phe (<i>p <</i> 0.05); in leg muscle, overfeeding decreased the contents of Asp, Glu, Ser, Ala, Tyr, Val, Phe, Ile and Leu, and increased the contents of Arg, His and Lys (<i>p <</i> 0.05). In fatty acids composition, the contents of C14:0, C16:1, C16:0, C18:2n6c, C18:1n9c, C18:0 and C20:0 of breast muscle significantly increased after overfeeding (<i>p <</i> 0.05). PCA and PLS-DA suggested that overfeeding had significant influence on the meat quality of the breast muscle and leg muscle.3. In conclusion, overfeeding improved the meat quality of overfed geese.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"468-478"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the genetic components of growth in ostriches with multi-trait and random regression models.","authors":"C Nel, A Gilmour, P Muvhali, S Cloete, M Kekana, A Engelbrecht","doi":"10.1080/00071668.2024.2442697","DOIUrl":"10.1080/00071668.2024.2442697","url":null,"abstract":"<p><p>1. The heritability (h²) of liveweight (LW) in ostriches can be highly variable, depending on age at recording. The objective of this study was to consider random regression (RR) as an alternative to the multi-trait (MT) structure for the analysis of repeated measures of LW.2. The data included 74 683 LW phenotypes recorded from 10 052 birds aged between 20 and 410 days (d) of age. Statistical analysis included single trait (ST), MT and RR analysis in a linear mixed model framework using the ASREML V4.2 software.3. For ST and MT, six traits were defined to represent LW at 28, 77, 150, 230, 300 and 365 d of age. Random variance components included direct genetic and maternal permanent environment (PE) effects. A MT analysis including all six traits converged.4. For RR, the data was transformed (LW + 10)^-0.5 due to difficulty in dealing with large scale effects. The final RR model fitted direct genetic and animal PE components as third degree Legendre polynomials and heterogeneous residuals.5. The h² estimates was in agreement across analysis, ranging from moderate (0.16-0.20) for W28 to high (0.41-0.51) for W230 to W365. Importantly, the genetic relationship between LW recorded as a chick and juvenile was only moderate (~0.35 to 0.55). The correlations between RR and MT EBVs for the six traits were 0.85, 0.54, 0.65, 0.75, 0.83 and 0.91, showing a considerable level of re-ranking.6. This study reaffirmed age dependent genetic variation when determining LW in ostriches. The RR structure was useful for overcoming the dimension problem of MT analysis, but was susceptible to scale effects present in the data, despite transformation. It remains unknown whether the need for cubic terms reflected scale or animal effects.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"429-438"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphism in the <i>MLANA</i> gene and its association with feather pigmentation in Chinese yellow quail (<i>Coturnix japonica</i>).","authors":"X Zhang, F Wu, J Hu, X Ji, Y Qi","doi":"10.1080/00071668.2025.2451243","DOIUrl":"10.1080/00071668.2025.2451243","url":null,"abstract":"<p><p>1. Melan-A <i>(MLANA)</i> plays a key role in the development of the melanosome, making it a strong candidate for the pigmentation phenotype observed in animals. The main purpose of this study was to analyse the relationship between <i>MLANA</i> gene polymorphisms and tyrosinase (TYR) enzyme activity in skin tissues and melanin content in dorsal down feathers of Chinese yellow quail.2. The coding sequence region of <i>MLANA</i> mRNA was cloned and sequenced to detect polymorphisms. The melanin content in down feathers of 266 Chinese yellow quails was analysed by spectrophotometry, and TYR enzyme activity was measured in dorsal skin tissues. The expression of <i>MLANA</i> mRNA in skin tissues of individuals with different genotypes was analysed using RT-qPCR.3. One non-synonymous single nucleotide polymorphisms (NSSNP; c.218T/A) was identified, which resulted in a Leu36Val mutation in the transmembrane helix region of the MLANA protein. This NSSNP significantly reduced the expression level of <i>MLANA</i> mRNA and TYR enzyme activity in dorsal skin tissues, leading to a significant reduction in melanin content in down feathers.4. The c.218T/A locus of the <i>MLANA</i> gene is closely related to the pigmentation TYR of the down feathers in Chinese yellow quail and can be used as a molecular marker locus for breeding pure feather colour in quail.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"453-457"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of pathogenicity factors of avian pathogenic and extraintestinal pathogenic <i>Escherichia coli</i> isolates originating from broiler chickens.","authors":"S Sariçam İnce, A Ünal, M Akan","doi":"10.1080/00071668.2025.2451242","DOIUrl":"10.1080/00071668.2025.2451242","url":null,"abstract":"<p><p>1. <i>E. coli</i> is an opportunist pathogen of animals, including food-producing ones and humans. Chickens may be a notable source of pathogenic and antimicrobial resistant <i>E. coli</i> for transmission to humans.2. This study compared virulence-associated genes (VGs) and antimicrobial resistance (AMR) in avian pathogenic <i>E. coli</i> (APEC) and extraintestinal pathogenic E. coli (ExPEC) isolates from broiler chickens, specifically APEC isolates in liver samples (<i>n</i> = 78) and ExPEC or non-ExPEC isolates in litter samples (<i>n</i> = 34). Virulence was evaluated by PCR for <i>feoB, hlyF, iroN, iss, iutA</i> and <i>ompT</i> genes, while AMR was evaluated by using antimicrobials from seven classes and detecting <i>bla_SHV, bla_TEM, bla_OXA, qnrB, stcM, mrc1, mrc2, sul1</i> and <i>tetA</i> genes.3. The APEC isolates were found in 100% of livers, while ExPEC and non-ExPEC isolates were found in 44% and 56% of the litter samples. The predominant VG was <i>feoB</i> (100%), followed by <i>ompT</i> (63%), <i>iutA</i> (60%), <i>iss</i> (58%) and <i>hlyF</i> (43%). Surprisingly, <i>iroN, omp T</i> and <i>iutA</i> had higher prevalences in APEC isolates (85%, 96% and 96%, respectively) than in ExPEC isolates (73%, 87% and 73%, respectively) and non-ExPEC isolates (0% for all). The presence of all VG in 33% of isolates indicated high pathogenicity.4. The isolates were phenotypically resistant to ampicillin (93%), ceftazidime (72%) and nalidixic acid (82%). All APEC and ExPEC isolates (100%) were multidrug resistant (MDR), while 63% of non-ExPEC isolates were MDR. Genotypic AMR testing revealed that 53% and 52% of all isolates had <i>stcM</i> and <i>tetA</i>, respectively. No isolate was positive for <i>bla_SHV, bla_OXA, mrc1</i> or <i>mrc2</i>, which suggested the benefits of colistin for treating carbapenem-resistant enteric pathogens, due to the high resistance detected to meropenem (47%).5. Given the potential pathogenicity of <i>E. coli</i> isolates, improving biosecurity practices in chicken flocks should be prioritised to eliminate transmission to humans through the food chain.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"515-522"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sensitive and rapid visual method of chicken sexing based on LAMP-CRISPR/Cas12a system.","authors":"Q Chen, C Su, S Li, Z Zhang, Y Yang, Y Yang, D Tao, S Xie, P Gong, Y Feng","doi":"10.1080/00071668.2025.2454963","DOIUrl":"10.1080/00071668.2025.2454963","url":null,"abstract":"<p><p>1. Accurate sex identification of one-day-old chicks is crucial in layer poultry production. Establishing an early sexing method during the chicken embryonic period is essential for animal welfare. However, PCR-based sexing has limitations in terms of specialised equipment and is time-consuming.2. This study presents a rapid, simple and fluorescent visual technique for chicken sex identification based on Loop-mediated isothermal amplification (LAMP)-clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12a (Cas12a). It targets the chicken Z chromosome gene DMRT1 and W chromosome-specific fragment EE0.6 using designed primers and sgRNA. The LAMP amplicon is cleaved by Cas12a, producing a fluorescent product detectable by a portable light apparatus.3. The method has high sensitivity, capable of detecting as few as two copies per microlitre of the EE0.6 template and 20 copies per microlitre of the DMRT1 template. This has significant potential for distinguishing chicken embryo gender very early in embryonic development.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"531-538"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between feeding regimen, NSPase enzyme and extent of grinding of barley-based pelleted diets on the performance, nutrient digestibility and ileal microbiota of broiler chickens.","authors":"K Itani, M Ahmad, S Ghimire, R B Schüller, J Apajalahti, A Smith, B Svihus","doi":"10.1080/00071668.2025.2451245","DOIUrl":"10.1080/00071668.2025.2451245","url":null,"abstract":"<p><p>1. The effects of feeding regimen, NSPase, extent of grinding and their interaction on the performance, digestive tract characteristics and ileal microbiota were studied. Eleven-day-old Ross 308 male broilers were given <i>ad libitum</i> (ADL) or intermittent (INT) access to finely (FG) or coarsely (CG) ground barley-based pelleted diets, with or without NSPase in a replicated pen trial. All birds had 4 h darkness separated with 1 h light periods with feed access. In addition, INT birds had access to feed through three 1 h feeding periods and one 2 h feeding period, with 3 h feed restriction periods in between.2. The INT feeding decreased weight gain (<i>p</i> < 0.001) but did not affect FCR. Supplementation with NSPase increased (<i>p</i> = 0.018) weight gain, but there was a tendency (<i>p</i> = 0.063) for it to be improved in INT-fed birds only. Including NSPase improved FCR, but only with FG diets (<i>p</i> = 0.037) and in INT group (<i>p</i> = 0.033).3. The CG diet significantly reduced (<i>p</i> = 0.044) pH of the gizzard contents and increased (<i>p</i> = 0.035) gizzard relative weight compared to FG. Addition of NSPase (<i>p</i> < 0.001) or FG (<i>p</i> = 0.049) reduced jejunal digesta viscosity. The FG diet improved (<i>p</i> = 0.019) starch digestibility compared to CG. In NSPase-supplemented diets, CG increased ileal protein digestibility compared to FG in birds fed ADL only, resulting in a three-way interaction(<i>p</i> = 0.012).4. The FG diet increased ileal concentration of total eubacteria and <i>Lactobacillus</i> spp. (<i>p</i> = 0.049), whilst INT feeding increased ileal concentration of S<i>treptococcus</i> spp. (<i>p</i> = 0.001). In NSPase-containing diets, FG increased ileal density of <i>Enterococcus</i> spp. in INT-fed birds (<i>p</i> = 0.027).5. In conclusion, finely-ground barley in pelleted diets responded better to NSPase enzymes than coarsely ground, particularly under INT feeding.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"558-569"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of encapsulated raspberry powder and phosphate on the lipid oxidation, microbiological properties and shelf life of modified atmosphere packaged chicken nuggets during chilled storage.","authors":"H Arslan, M I Aksu","doi":"10.1080/00071668.2025.2453992","DOIUrl":"10.1080/00071668.2025.2453992","url":null,"abstract":"<p><p>1. Further-processed poultry products, such as chicken nuggets, are susceptible to rapid lipid oxidation and microbial spoilage. Natural ingredients from various plants or fruits may contribute to improving the quality and extending the shelf life of meat products. In the present study, the use of encapsulated raspberry powder (ERP; control, 0.5%, 1.0%) in chicken nugget production with and without phosphate (0.0%, 0.3%) and its effects on chemical composition, lipid oxidation, microbial quality and shelf life were examined.2. Phosphate and ERP had effects on chemical composition and a_w; (<i>p</i> < 0.01). During storage, the ratio of O₂ and N₂ increased and the amount of CO₂ decreased (<i>p</i> < 0.05), but the increase in samples with 1.0% ERP addition was lower than in the control and 0.5% groups.3. Both the use of ERP (<i>p</i> < 0.01) and phosphate (<i>p</i> < 0.01) for nugget production prevented lipid oxidation. The lowest was determined in samples containing phosphate + 1.0% ERP (<i>p</i> < 0.05) during storage. However, the TBARS values were within acceptable limits (<1 mg MDA/kg) for all the samples with 0.5% and 1.0% ERP added with phosphate.4. The counts of total aerobic mesophilic bacteria decreased depending on the level of ERP added to the nugget composition (<i>p</i> < 0.01). Counts only exceeded 6 log CFU/g in the control samples on the 120^th day of storage. <i>Salmonella</i> spp. and <i>Listeria monocytogenes</i> were negative in all nugget samples during storage. The counts of <i>Enterobacteriaceae</i> were below the detectable limit (<2.0 log CFU/g) during storage.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"489-504"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of the CRISPR-Cas systems in <i>Enterococcus faecalis</i> from commercial broiler farm environments and its association with antimicrobial resistance.","authors":"M B Kim, Y J Lee","doi":"10.1080/00071668.2025.2451268","DOIUrl":"10.1080/00071668.2025.2451268","url":null,"abstract":"<p><p>1. Clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins (Cas) systems have been highlighted for their potential applications in controlling the spread of mobile genetic elements, including antimicrobial resistance (AMR) genes. This study investigated the characteristics of CRISPR-Cas systems in <i>E. faecalis</i> from commercial broiler farms and assessed the impact of these systems on AMR.2. All <i>E. faecalis</i> isolates contained CRISPR2, and CRISPR1-Cas and CRISPR3-Cas were identified in 84 (56.4%) and 144 (96.6%) isolates. A combination of CRISPR2 and CRISPR3-Cas and a combination of CRISPR1-Cas, CRISPR2 and CRISPR3-Cas were each identified in 27 (96.4%) farms.3. There were significant differences between CRISPR-Cas systems for phenotypic AMR: CRISPR1-Cas and CRISPR3-Cas. The <i>E. faecalis</i> isolates without CRISPR1-Cas showed higher resistance to most antimicrobials and had a higher prevalence of multidrug resistance (MDR) than those with CRISPR1-Cas. However, the resistance rate against most antimicrobials and the prevalence of MDR did not differ significantly depending on the presence or absence of CRISPR3-Cas.4. The <i>E. faecalis</i> isolates without CRISPR1-Cas harboured higher levels of all AMR genes, except for <i>tetL</i>, than those with CRISPR1-Cas. However, the <i>E. faecalis</i> isolates with CRISPR3-Cas showed a significant lower prevalence of <i>tetL</i> gene and a significantly higher prevalence of <i>fexA</i> and <i>poxtA</i> genes.5. In the distribution of <i>rep</i> families, the <i>rep</i>₉ family was predominant, followed by <i>rep</i>₁, <i>rep</i>₇, <i>rep</i>₂ and <i>rep</i>₈ families. Only prevalence of the <i>rep</i>₇ family was significantly higher in the <i>E. faecalis</i> isolates without CRISPR1-Cas (15.4%) than in those with CRISPR1-Cas (0%).6. This study is the first report on the characteristics of CRISPR-Cas systems in <i>E. faecalis</i> isolated from commercial broiler farm environments, and the results supported the hypothesis that the development of antimicrobial strategies requires an understanding of the distinctive capabilities between CRISPR1-Cas and CRISPR3-Cas and their underlying resistance mechanisms.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"523-530"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of <i>Proteus mirabilis</i> isolates from the poultry production chain in Zhejiang Province, China: antimicrobial resistance, virulence factors and genotypic profiling.","authors":"Y Xu, X Ji, X Chen, G Gui, T He, Y Xiao, L Lv, W Lyu","doi":"10.1080/00071668.2024.2436995","DOIUrl":"10.1080/00071668.2024.2436995","url":null,"abstract":"<p><p>1. This study investigated antimicrobial resistance, virulence factors and genotypic profiling among <i>Proteus mirabilis</i> isolated from three sources (poultry farms, slaughterhouses and retail markets) in the poultry production chain in Zhejiang Province, China, to assess its potential risk to public health.2. A total of 112 <i>P. mirabilis</i> strains were isolated from 409 samples, including 35 from poultry farms, 35 from slaughterhouses and 42 from retail markets. Antimicrobial susceptibility was tested using 18 antimicrobials in 9 categories, in which 110 (98.2%) strains were considered multidrug-resistant (MDR). These strains carried numerous antimicrobial resistance genes, with the sulphonamide resistance gene (<i>sul1</i>) having the highest rate (100%) and the polymyxin resistance gene (<i>mcr-1</i>) the lowest (3.6%).3. These isolates were validated to carry virulence genes <i>pmfA</i>, <i>mrpA</i>, <i>atfC</i>, <i>rsbA</i>, <i>atfA</i>, <i>ureC</i> and <i>ucaA</i> with the high prevalence of 96.4, 92.9, 92.0, 85.7, 85.7, 57.1 and 46.4%, respectively. Genotyping results using the ERIC-PCR indicated that the genetic similarity of all the isolates was 68.6% to 100% which fell into 4 clusters.4. The <i>P. mirabilis</i> isolates from the slaughterhouses exhibited higher levels of antibiotic resistance and a more pronounced MDR phenomenon than those from poultry farms and retail markets. The proportion of isolates carrying the most commonly detected resistant and virulence genes was higher in samples from poultry farms and slaughterhouses as opposed to retail markets. Importantly, there was genetic similarity and heterogeneity among <i>P. mirabilis</i> isolates from the three sources and genotypic diversity was the highest among isolates from retail markets, followed by slaughterhouses and poultry farms.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"505-514"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics uncover acute stress vulnerability through gut-hypothalamic communication in ducks.","authors":"T Gu, R Guo, L Chen, Y Zong, Y Tian, W Xu, T Zeng, L Lu","doi":"10.1080/00071668.2025.2454960","DOIUrl":"10.1080/00071668.2025.2454960","url":null,"abstract":"<p><p>1. The avian gut hosts a complex and dynamic microbial ecosystem, which is essential for regulating host organ function. However, the relationship between the gut microbiota and the hypothalamic axis in acute stress vulnerability in ducks remains unclear.2. This study investigated how the gut microbiota affects microbial metabolism and the host stress response by comparing hypothalamic neurotransmitter availability, microbial composition and co-metabolites generated by both the microbiota and hypothalamus in ducks exhibiting the lowest active avoidance (LAA) and highest active avoidance (HAA) behaviour.3. The HAA group experienced a significant increase in the availability of arginine, histidine, glutamine, norepinephrine, L-tyrosine and melatonin during acute stress in the hypothalamus, compared to that in the LAA group. The 16S rRNA sequencing revealed significant differences in the gut microbiota composition based on acute stress vulnerabilities.4. Both caecal and hypothalamic metabolomic analyses identified 71 metabolites altered in caecal content and 95 in the hypothalamus. There was significant enrichment in pathways such as the cGMP-PKG signalling, dopaminergic synapse and endocrine resistance.5. Correlation analyses demonstrated that certain co-metabolites, including 1,3-dicyclohexylurea, 1-deoxyvaleric acid, 2-amino-2-methyl-1,3-propanediol, 3-chloroaniline, methenamine, N4-acetylcytidine-triphosphate and traumatin, may play a role in the gut microbiota-hypothalamic axis.6. The results suggested that the gut microbiome influenced acute stress responses. This provided a basis for understanding gut-hypothalamic communication and its impact on behaviour in ducks.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"479-488"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}