British Poultry Science最新文献

{"title":"Integrative 'omics' analysis elucidates the role of the gene <i>ANKRD9</i> in modulating chicken primary myoblast IMP metabolism <i>via</i> the purine metabolic pathway.","authors":"Y Li, P Yuan, R Wu, B Zhai, H Li, J Shen, B Chen, Z Li, W Li, Y Tian, X Liu, X Kang, Y Wang, G Li","doi":"10.1080/00071668.2026.2625885","DOIUrl":"10.1080/00071668.2026.2625885","url":null,"abstract":"<p><p>1. This study used the Chinese local breed, Gushi chicken, as the model to investigate the ankyrin repeat domain 9 (<i>ANKRD9</i>) gene. This focussed on its biological function in muscle development and regulation of inosine monophosphate (IMP), a key flavour metabolite in chicken meat.2. The dynamic expression of <i>ANKRD9</i> in breast muscle tissue was analysed using qRT-PCR. Combined with transcriptomic and metabolomic technologies, the regulatory network of <i>ANKRD9</i> in chicken primary myoblasts (CPM) was elucidated.3. The results indicated that <i>ANKRD9</i> likely affected cellular adhesion and IMP accumulation. The ELISA assays in CPM confirmed that overexpression of <i>ANKRD9</i> significantly inhibited IMP metabolism in myoblasts. This suggested this gene has a key role in the negative regulation of IMP.4. These results highlighted the critical role of <i>ANKRD9</i> in regulating the flavour metabolite IMP. This provides a potential molecular target and theoretical foundation for the breeding of high-quality broilers.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"719-727"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147324478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling conserved and breed-specific RNA expression signatures underlying hepatic homoeostasis across genetically diverse chicken breeds.","authors":"M Bagiyal, S Ahlawat, R Arora, R Sharma, S Khatak, P Chhabra, R Parsad, S Malhotra, N Mohan","doi":"10.1080/00071668.2026.2621259","DOIUrl":"10.1080/00071668.2026.2621259","url":null,"abstract":"<p><p>1. The liver plays a key role in lipid metabolism, immune regulation and energy balance. To better understand the fundamental molecular mechanisms that support liver function, this study investigated hepatic transcriptomes from four genetically diverse chicken breeds: Kadaknath, Aseel, Punjab Brown or commercial broilers.2. The RNA sequencing revealed 11938 genes that were commonly expressed across all breeds. From these, the top 1000 most abundantly expressed genes were analysed in depth. In addition, breed-specific genes were identified, including 53 in Kadaknath, 63 in Aseel, 498 in broiler and 45 in Punjab Brown chickens.3. The GO enrichment analysis revealed that the conserved hepatic genes were mainly involved in haemostasis, immune response, lipid metabolism, amino acid metabolism, oxidative stress regulation and protein quality control. Pathway analysis highlighted key pathways including PPAR signalling, unsaturated fatty acid biosynthesis, complement and coagulation cascades, ER-associated degradation, glutathione metabolism and iron homoeostasis, reflecting core liver functions.4. Functional interpretation of the identified hepatic genes indicated associations with pigmentation and muscle-related functions in Kadaknath, neuroendocrine and behaviour-associated functions in Aseel, growth and muscle development functions in broilers and reproduction-related functions in Punjab Brown birds. This highlighted distinct genetic architectures underlying breed-specific phenotypes.5. This study defined a conserved hepatic transcriptional landscape shared across chicken breeds, alongside breed-specific gene expression patterns, providing a foundation for future functional studies.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"707-718"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary inclusion of black soldier fly larvae as a partial protein source: effects on growth performance, carcass traits and meat quality of broilers.","authors":"Q Jiang, S Chen, Y Li, Z Zhou, M Zhang, Y Sun, L Zhang, Y Li, X Zhang, Y Qu, Z Lv","doi":"10.1080/00071668.2026.2620609","DOIUrl":"10.1080/00071668.2026.2620609","url":null,"abstract":"<p><p>1. Black soldier fly larvae (BSF) can convert kitchen waste (KW) into usable organic resources. This study evaluated the effects of BSF derived from kitchen waste on the growth performance, carcass traits, meat quality and caecal microbiota in broilers.2. A total of 180 broilers were randomly assigned to three groups: a control group (CON), LBF or HBF group (containing 3% and 6% of BSF respectively). Performance was measured as feed intake (FI), Average daily weight gain (ADG), allowing feed conversion ratios (FCR) to be calculated. Carcass and muscle yields and characteristics were recorded. Caecal samples were taken for analysis of populations.3. The results showed that the 3% BSF group had better performance, including significant reductions in FI and abdominal fat and increased breast meat yield and muscle fibre diameter (<i>p</i> < 0.05). The mRNA levels of myostatin (<i>MSTN</i>), muscle RING finger protein 1 (<i>MuRF1</i>) and muscle atrophy F-box (<i>MAFbx</i>) were significantly downregulated (<i>p</i> < 0.05). The LBF group showed significantly elevated levels of specific fatty acids in breast meat, such as lauric acid and myristoleic acid (<i>p</i> < 0.05). However, replacing soybean meal with BSF in diet reduced meat quality and increased cooking loss and toughness (<i>p</i> < 0.05).4. The 16S rRNA microbiome sequencing showed that the LBF birds had significant increases in beneficial bacteria, including <i>Butyricicoccus pullicaecorum</i> and <i>Akkermansia muciniphila</i> and reduced <i>Alistipes finegoldii</i> abundance (<i>p</i> < 0.05). Spearman correlation analysis showed that <i>Butyricicoccus pullicaecorum</i> was positively associated with breast meat yield, muscle fibre size and cooking loss and negatively associated with the expression of <i>MSTN</i>, <i>MuRF1</i> and <i>MAFbx</i>. <i>Alistipes finegoldii</i> showed opposite correlated trends to these indicators.5. In conclusion, dietary inclusion of BSF can regulate gut microbial composition and the expression of genes related to muscle development, thereby improving meat production, but affecting meat quality. Additionally, BSF alter the fatty acid profile of breast meat.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"859-870"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitogenomic characterisation of the Ispenc chicken: a genetically distinct Turkish miniature breed.","authors":"D Özdemir, E Toparslan Akcay, L Bener, K Karabag","doi":"10.1080/00071668.2026.2630729","DOIUrl":"10.1080/00071668.2026.2630729","url":null,"abstract":"<p><p>1. The Ispenc chicken, a genetically undefined miniature breed in Türkiye, was analysed using mitochondrial DNA to clarify its maternal lineage and assess its distinction from European ornamental breeds (Sabelpoot and Barbu d'Anvers) as well as from the Sultan breed.2. Phylogenetic analysis of a 1508 bp mtDNA fragment spanning the D-loop identified five haplotypes (H1-H5): Ispenc individuals carried H2 and H3, Sultan chickens carried H4, while Sabelpoot and Barbu d'Anvers shared H5.3. Molecular variance analysis indicated strong population structure, with 98% of genetic variation occurring among populations (F_st = 0.98184). Two complete mitogenomes representing the H2 and H3 haplotypes of the Ispenc breed (16785 bp each) were assembled, each containing a 1232 bp D-loop and the standard set of 37 mitochondrial genes.4. Comparative mitogenome analysis identified 62 nucleotide differences among haplotypes: Ispenc differed from Sabelpoot and Barbu d'Anvers by 44-45 nucleotides, whereas these two breeds differed by only two nucleotides. Haplogroup analysis assigned Ispenc to haplogroup A and the European ornamental breeds to haplogroup E.5. These results demonstrated that the Ispenc chicken represents a distinct maternal lineage that is clearly differentiated from European miniature breeds. This underscored its value as a native genetic resource and highlighting the need to conserve both of its mtDNA lineages (H2 and H3).</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"728-735"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation combining apo-carotenoid acid ester and canthaxanthin can counteract any decline in egg albumen quality.","authors":"M Bozkurt, Y Ögmen, A Ö Üstündağ","doi":"10.1080/00071668.2026.2634793","DOIUrl":"10.1080/00071668.2026.2634793","url":null,"abstract":"<p><p>1. The utilisation of carotenoids in hen diets can be inversely related to the dietary fibre content. The influence of dietary crude fibre level (CFL) and carotenoid supplementation (CRS) on laying and egg quality was studied in commercial layer hens from 51 to 60 weeks of age. The CRS was included as a blend of 0.5 mg of apo-carotenoid acid ester and 2.5 mg of canthaxanthin per kg of diet.2. The experimental design was a 2 × 2 factorial with two CFL (5.2% vs. 6.5%) and CRS levels (a basal diet with or without CRS). A total of 240 Hisex Brown laying hens were randomly assigned to one of the four treatment groups, with six replicates of 10 hens each.3. Laying performance and serum concentrations of triglyceride, cholesterol, carotenoid, malondialdehyde, superoxide dismutase and glutathione peroxidase were not significantly influenced by CFL and CRS.4. An increase in CFL reduced egg yolk index by 3.5% (<i>p</i> < 0.01) and increased eggshell thickness by 3.0% (<i>p</i> < 0.01). Supplementing feed with CRS caused a 5.1% decrease (<i>p</i> < 0.05) in eggshell breaking strength, while yolk index showed a positive response with a 2.6% increase (<i>p</i> < 0.01).5. Both CFL and CRS interacted (<i>p</i> < 0.01) on the albumen index, whereby CRS improved albumen index by 12% in hens fed a diet containing 6.5% CFL, whereas only a 3% increase was observed when CRS when CFL was added at 2%.6. The link between dietary fibre, carotenoid utilisation and internal egg quality highlighted the importance of considering the beneficial effects of supplemental carotenoids on albumen quality when increasing CFL above 6%. This can help address current concerns about the increase in fibre content in hen diets.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"919-928"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147632337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of glyphosate in feed on egg hatchability and health of chicks and breeder hens.","authors":"M T Sørensen, T S Dalgaard, R B Kjærup, D Mandrioli, L Foldager","doi":"10.1080/00071668.2026.2615732","DOIUrl":"10.1080/00071668.2026.2615732","url":null,"abstract":"<p><p>1. This study investigated the potential effects of exposure to glyphosate from feed on breeder hens on egg hatchability and chick health. Hens received diets with no added glyphosate (Gly as a control (Con), or with addition of 1.5 (Gly_1.5), 4.5 (Gly_4.5) and 13.5 (Gly_13.5) mg/kg glyphosate in the diet resulting in analysed contents of 0.01, 1,47, 3.59 and 11.6 mg per kg diet, respectively.2. There were no effects of glyphosate in diets fed to breeder hens on hatchability of fertilised eggs (0.95, 0.93, 0.91 and 0.90 for Con, Gly_1.5, Gly_4.5 and Gly_13.5, respectively). Even though no effects of glyphosate were observed on one-day-old progeny chick quality, a <i>post hoc</i> analysis indicated that the navel score was best for chicks from the Con group compared to chicks from the combined Gly groups (<i>p</i> = 0.025). Weight gain of the chicks at 8 d of age was not affected.3. For immune competence and leukocyte subset counts in whole blood from breeder hens and one-day-old chicks, the results showed higher numbers of B-cells (<i>p</i> = 0.005) and monocytes (<i>p</i> = 0.027) as well as phagocytic activity of lymphocytes (<i>p</i> = 0.015). These were associated with higher glyphosate levels in peripheral blood from hens, whereas only phagocytic activity of lymphocytes was seen in chicks (<i>p</i> = 0.004). Total antioxidant capacity in blood plasma of hens and one-day old chicks was not affected.4. In conclusion, only some immune changes were associated in both hens and chicks with up to 11.6 mg glyphosate/kg diet fed to breeder hens.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"751-760"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146140803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the interactive effect of dietary fat and feedstuff types on basal endogenous amino acid flow, coefficients of standardised ileal amino acid digestibility, the morphology of small intestine and jejunal mucosa gene expression in broiler chickens.","authors":"S Veeraganti, B Kasireddy, O A Olukosi","doi":"10.1080/00071668.2026.2616776","DOIUrl":"10.1080/00071668.2026.2616776","url":null,"abstract":"<p><p>1. The objective of Experiment 1 was to determine the effect of fat source on basal endogenous losses of amino acids (BEL), histomorphology of the small intestine and caecal short-chain fatty acid (SCFA). Experiment 2 was conducted to determine the interactive effect of feedstuffs and fat types on coefficients of standardised ileal amino acid digestibility (cSIAAD) of maize and soybean meal (SBM), histomorphology of jejunum and ileum and the relative gene expression of amino acid transporters in the jejunal mucosa.2. In Experiment 1, a total of 80 broiler chickens were allocated to 16 metabolic cages (two diets, eight replicates each). Two nitrogen-free diets were formulated with soybean oil (25 g/kg, NFDS) or tallow (35 g/kg, NFDT) as fat sources. A total of 160 broiler chickens were allocated to 32 metabolic cages (four diets, eight replicates each) in Experiment 2. Four semi-purified diets consisted of maize or SBM as the sole protein source, each supplemented with either soybean oil or tallow as the fat type.3. In Experiment 1, there was no significant effect for any of the responses. In Experiment 2, there were no feedstuff × fat type interactions for any of the responses. The cSIAAD for Leu, Ala, Cys and Glu were greater (<i>p</i> < 0.05) for maize. The jejunal and ileal villi were longer, ileal crypts deeper and the relative expressions of peptide and amino acid transporter genes were greater (<i>p</i> < 0.05) for birds that received the SBM diets.4. In conclusion, under the conditions of this experiment, cSIAAD values determined with assay diets using the two fat types were similar.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"838-846"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benzoic acid and oregano essential oil interact to increase the immune function and intestinal development of Langshan chickens.","authors":"H Peng, L Zhang, L Zhao, T Xing, F Gao","doi":"10.1080/00071668.2026.2619842","DOIUrl":"10.1080/00071668.2026.2619842","url":null,"abstract":"<p><p>1. This study was conducted to investigate the effects of dietary supplementation with benzoic acid (BA) and oregano essential oil (OEO) on the growth, performance, immune function and intestinal development of Langshan chickens.2. A total of 240 Langshan chickens were randomly allocated into five treatment groups in a 42 d trial (aged 30-72 d): Basal diet (CON); basal +50 mg/kg chlortetracycline (CTC); basal +2,000 mg/kg BA (BA); basal +300 mg/kg OEO (OEO); basal +2,000 mg/kg BA and 300 mg/kg OEO (BA+OEO).3. Compared with CON the BA, OEO and BA+OEO groups elevated jejunal goblet cell numbers (<i>p</i> < 0.05) and lowered serum interleukins IL-1β, IL-6, IL-8 and TNF-α at both 51 and 72 d of age (<i>p</i> < 0.001). The BA+OEO group altered caecal microbiota beta-diversity (<i>p</i> < 0.001) and increased <i>Alistipes</i> and <i>Faecalibacterium</i> spp. abundance compared to CON and CTC (<i>p</i> < 0.05).4. These results suggested that supplementing BA with OEO gave better responses than chlortetracycline in enhancing immune responses and intestinal health.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"847-858"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics reveals associations between feed efficiency, physiological traits and meat quality in slow-growing chickens.","authors":"Y Wang, C Hu, J Cai, L Lu, D Zhang, H Peng, W Luo","doi":"10.1080/00071668.2026.2632914","DOIUrl":"10.1080/00071668.2026.2632914","url":null,"abstract":"<p><p>1. A total of 450 male chickens were individually housed and assessed for residual feed intake (RFI) and feed conversion ratio (FCR) over a 28 d feeding trial, with subsequent phenotypic measurements and breast muscle samples collected from selected high- and low-efficiency groups for metabolomic and lipidomic profiling.2. Residual FI had significant correlations with faecal volume, behavioural activity and head temperature, while FCR showed only a negative correlation with head temperature. Meat quality analysis revealed that residual FI correlated with breast muscle shear force, while FCR was associated with thigh muscle colour parameters.3. Multi-omics profiling of breast muscle showed distinct metabolite and lipid compositions between efficiency groups, and FCR was linked to triacylglycerol content and residual feed intake (RFI) to glycerophospholipid levels.4. This trial demonstrated that feed efficiency influences both physiological processes and meat quality, providing a basis for balanced breeding strategies in slow-growing poultry.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"736-750"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of lipopolysaccharide and zymosan on amino acid metabolism in chicks.","authors":"A Yamada, M Takahashi, T Ishida, S Khan, M A Cline, T Tachibana","doi":"10.1080/00071668.2026.2614288","DOIUrl":"10.1080/00071668.2026.2614288","url":null,"abstract":"<p><p>1. This study investigated the metabolic effects of lipopolysaccharide (LPS, a pathogen-associated molecular pattern derived from gram-negative bacteria) and zymosan (a pathogen-associated molecular pattern derived from fungi) in young chickens (<i>Gallus gallus domesticus</i>).2. Liver RNA-seq analysis was performed on samples from chicks that received intraperitoneal injections of either LPS or zymosan. Pathogen-associated molecular patterns (PAMP) altered the expression of several genes associated with lipid, amino acid and carbohydrate metabolism in the liver. Moreover, both PAMP affected a common set of genes, with changes in their expression exhibiting a strong positive correlation (<i>R</i> ≥ 0.90). Subsequent analyses revealed that both PAMP altered plasma concentrations of several amino acids, including histidine.3. Finally, both PAMP significantly increased the mRNA expression of histidine decarboxylase and carnosine-N-methyltransferase, which are enzymes involved in the synthesis of histamine and anserine, respectively, in the liver and pectoralis major.4. These findings suggested that LPS and zymosan affect several metabolic pathways, particularly those related to amino acid metabolism and that they likely act through overlapping metabolic mechanisms.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"827-837"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}