British Poultry Science最新文献

{"title":"Interplay of poultry-microbiome interactions - influencing factors and microbes in poultry infections and metabolic disorders.","authors":"C E Aruwa, S Sabiu","doi":"10.1080/00071668.2024.2356666","DOIUrl":"10.1080/00071668.2024.2356666","url":null,"abstract":"<p><p>1. The poultry microbiome and its stability at every point in time, either free range or reared under different farming systems, is affected by several environmental and innate factors. The interaction of the poultry birds with their microbiome, as well as several inherent and extraneous factors contribute to the microbiome dynamics. A poor understanding of this could worsen poultry heath and result in disease/metabolic disorders.2. Many diseased states associated with poultry have been linked to dysbiosis state, where the microbiome experiences some perturbation. Dysbiosis itself is too often downplayed; however, it is considered a disease which could lead to more serious conditions in poultry. The management of interconnected factors by conventional and emerging technologies (sequencing, nanotechnology, robotics, 3D mini-guts) could prove to be indispensable in ensuring poultry health and welfare.3. Findings showed that high-throughput technological advancements enhanced scientific insights into emerging trends surrounding the poultry gut microbiome and ecosystem, the dysbiotic condition, and the dynamic roles of intrinsic and exogenous factors in determining poultry health. Yet, a combination of conventional, -omics based and other techniques further enhance characterisation of key poultry microbiome actors, their mechanisms of action, and roles in maintaining gut homoeostasis and health, in a bid to avert metabolic disorders and infections.4. In conclusion, there is an important interplay of innate, environmental, abiotic and biotic factors impacting on poultry gut microbiome homoeostasis, dysbiosis, and overall health. Associated infections and metabolic disorders can result from the interconnected nature of these factors. Emerging concepts (interkingdom or network signalling and neurotransmitter), and future technologies (mini-gut models, cobots) need to include these interactions to ensure accurate control and outcomes.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"523-537"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial budget analysis of laying hens' transition from cages to production systems of improved welfare: a case study in Greece.","authors":"M Z Kritsa, K Tsiboukas, E N Sossidou, P E Simitzis, M Goliomytis","doi":"10.1080/00071668.2024.2360631","DOIUrl":"10.1080/00071668.2024.2360631","url":null,"abstract":"<p><p>1. In recent years, public concern regarding animal welfare has increased while, at the same time, cage systems for animal production have been greatly criticised by EU citizens. In addition, large food retailers promote food products that are made with eggs produced in cage-free systems.2. The objective of this study was to evaluate the economic viability of the transition of laying hens' to production systems; from conventional to alternative systems with improved welfare. Three independent scenarios were assumed as follows: transition from enriched colony cages to barn (S1), transition from barn to free-range (S2), and transition from free-range to organic (S3). Economic assessments of each transition was applied to a model farm in Greece with 12 000 hens, through partial budget analysis and net benefits and costs were estimated.3. The results showed a positive economic impact in all transitions to a production system of improved animal welfare (€12,044 in S1, €18,957 in S2 and €7,977 in S3) which indicated that they are economically sustainable. In all scenarios, unit cost increased by 19% in S1, 12% in S2, and 85% in S3.4. In conclusion, transitioning towards improved animal welfare production systems in laying hen farms could be an economically viable option for egg producers in compliance with societal demands and market trends.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"503-512"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and transcriptomic analysis of the ovary during laying and brooding stages in Zhedong white geese (<i>Anser cygnoides domesticus</i>).","authors":"Y Wang, S Wang, Z Zang, B Li, G Liu, H Huang, X Zhao","doi":"10.1080/00071668.2024.2364351","DOIUrl":"10.1080/00071668.2024.2364351","url":null,"abstract":"<p><p>1. This study investigates the molecular mechanisms affecting brooding in Zhedong white geese by examining differences in reproductive endocrine levels, ovarian histology and transcriptomics.2. Twenty 18-month-old Zhedong white geese were selected to examine their ovaries using histological, biochemical, molecular biological, and high-throughput sequencing techniques during the laying and brooding periods.3. The results showed that the number of atretic follicles and apoptotic cells in the ovaries increased significantly (<i>p</i> < 0.05), the levels of follicle-stimulating hormone, luteinising hormone, gonadotropin-releasing hormone and oestradiol decreased significantly (<i>p</i> < 0.05), and the level of prolactin increased significantly (<i>p</i> < 0.01) during the brooding stage.4. In broody geese, the expression of <i>CASP3, CASP9, P53, BAX</i>, and <i>Cyt-c</i> were considerably higher (<i>p</i> < 0.05), but <i>BCL2</i> expression was significantly lower (<i>p</i> < 0.05).5. In ovarian tissues, 260 differentially expressed lncRNAs, 13 differentially expressed miRNA and 60 differentially expressed mRNA were all discovered using transcriptome sequencing analysis. Functional enrichment analysis revealed that the differentially expressed mRNA and non-coding RNA target genes were primarily involved in ECM-receptor interaction, cell adhesion, cardiac muscle contraction, mTOR signalling, and the calcium signalling pathway.6. In conclusion, follicular atrophy and apoptosis occurred in the ovaries and serum reproductive hormone levels were significantly changed during the brooding period of Zhedong white geese. <i>COL3A1, COL1A2, GRIA1, RNF152</i>, miR-192, and miR-194 may be important candidates for the regulation of brooding behaviour, with the mTOR signalling pathway playing a key role.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"631-644"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of grinding method and extent of pelleting of broiler diets on performance, feeding behaviour and digestive tract functionality.","authors":"S Dhakal, H Hetland, B Svihus","doi":"10.1080/00071668.2024.2405208","DOIUrl":"10.1080/00071668.2024.2405208","url":null,"abstract":"<p><p>1. An experiment was carried out to examine if a heterogeneous diet consisting of pellets and coarse cereal particles would result in a similar feed intake to pelleted-only diets. It also considered whether coarser particles entering the gizzard would encourage the development of the gizzard and thus improve digestive tract functionality. Maize and wheat were hammer milled through a 3 mm screen after coarse grinding using either one of the two screens with a novel pattern. Three pellet-only diets were produced based on three grinding sizes. Additionally, four diets were produced by separating coarse cereal particles from moderately coarse particles by sieving either once or twice, pelleting the remainder and finally mixing these into a complete heterogeneous diet.2. No difference in performance between birds fed the three pelleted-only diets was observed. However, heterogeneous diets resulted in a lower feed intake (FI) and weight gain (WG) than pelleted-only diets (<i>p</i> < 0.05). The dietary treatments neither affected the feed conversion ratio nor ileal digestibility of starch and protein (<i>p</i> > 0.05). As expected, feeding a heterogeneous diets increased (<i>p</i> < 0.05) the relative weight of the gizzard and its contents. Approximately 50% of the particles in the small intestine were between 0.15 mm and 0.36 mm in diameter, with more fine particles observed when the pelleted-only diets containing coarsely ground cereals were fed, confirming the capacity of the gizzard to finely grind feed.3. Sieving of the diet remaining in the feeder during 4 h of feeding two of the heterogeneous diets showed that the particles > 2.8 mm disappeared first (<i>p</i> < 0.05), showing the birds' preference for larger particles when offered heterogeneous diets. Although the birds were able to handle the coarse microstructure of the diets without any negative effects, the reduced performance in the case of heterogeneous diets might have been related to feed intake issues and particle selection, resulting in an imbalance in nutrient intake.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142342017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycerol is not suitable for the cryopreservation of quail semen.","authors":"I Grasseau, I Couty, I Grimaud-Jottreau, A Vitorino Carvalho","doi":"10.1080/00071668.2024.2357671","DOIUrl":"10.1080/00071668.2024.2357671","url":null,"abstract":"<p><p>1. This study investigated the potential use of glycerol as a cryoprotectant for quail sperm cells. Its role in maintaining sperm fertilising ability <i>in vivo</i> and <i>in vitro</i> quality parameters, such as motility, was assessed.2. The data showed that the presence of glycerol in semen samples was associated with infertility, which suggested that removal prior to insemination is mandatory. Removal through serial dilution centrifugation was associated with fewer than 5% of motile sperm cells and resulted in no fertility.3. In conclusion, glycerol alone is not suitable for quail semen cryopreservation, and other approaches need to be investigated to develop cryobanking programmes for this species.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"625-630"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel circular RNA <i>Sestrin</i>1 promotes chicken myoblast proliferation and differentiation via circSesn1/miR-16-5p/SESN1 pathway.","authors":"X Shen, J Liao, S Yu, P Feng, G Wang","doi":"10.1080/00071668.2024.2360004","DOIUrl":"10.1080/00071668.2024.2360004","url":null,"abstract":"<p><p>1. The development of chicken skeletal muscle is directly relevant to poultry husbandry production. Numerous studies have suggested that circular RNA play pivotal roles in muscle development. However, the functions and mechanisms of most circRNA in chicken myogenesis remain largely unknown.2. This study identified a novel circSESN1 based on existing sequencing data and examined its authenticity and subcellular localisation by enzyme digestion and RNA fluorescence in situ hybridisation. Additionally, there was a positive correlation between the expression levels of circSESN1 and the developmental stage of chicken muscle.3. Mechanistically, knockdown or overexpression of circSESN1 was performed in primary myoblasts to validate its function. The interactions between circSESN1, miR-16-5p, and the target gene <i>sestrin</i> 1 (<i>SESN</i>1) were investigated using bioinformatics analysis and a dual fluorescein reporter system. Real-time qPCR, a cell proliferation assay, and immunofluorescence staining techniques were used to investigate the promotion effect of circSESN1 on myoblast proliferation and differentiation by miR-16-5p/<i>SESN</i>1 pathway.4. The results demonstrated that the newly identified chicken circSESN1 directly sponges gga-miR-16-5p to regulate <i>SESN</i>1 gene expression, promoting myoblast proliferation and differentiation.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"513-522"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141198629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Properties of encapsulated raspberry powder and its efficacy for improving the pH, colour quality and shelf life of modified atmosphere packaged chicken nuggets.","authors":"M I Aksu, H Arslan","doi":"10.1080/00071668.2024.2359988","DOIUrl":"10.1080/00071668.2024.2359988","url":null,"abstract":"<p><p>1. Recent research has shown that encapsulated raspberry powder (RP) is a natural colourant for foodstuffs. However, no research has been conducted on its use in chicken nuggets. In addition, the effect of RP on products with and without phosphate addition is unknown. This study assessed the effects of RP (control, 0.5%, 1.0%) and phosphate (0.0%, 0.3%) on the pH and colour quality properties of nuggets.2. In the production of RP, red raspberry (<i>Rubus ideaus</i> L.) juices were encapsulated using maltodextrin in a spray-dryer. Antioxidant activity, total anthocyanin, total phenolics, colour, moisture and pH analyses of the RP were performed.3. Nuggets were packaged in modified atmosphere packaging (MAP; 40%CO₂ + 60%N₂) and were stored at 2.0 ± 0.5°C for 120 d. The pH and external and internal surface colour (L*, a*, b*, C* and h) values were measured on d 0, 15, 30, 45, 60, 75, 90, 105 and 120 of storage.4. The addition of phosphate increased the pH in the samples, while these decreased with the addition of RP (<i>p</i> < 0.05). During storage, the highest pH were seen in the phosphate samples and the lowest in the nuggets with 1.0% RP addition (<i>p</i> < 0.05).5. With the addition of phosphate, the external surface a* value of nuggets increased (<i>p</i> < 0.05). Depending on the level of RP added to the nuggets, the external surface L* value decreased and a* and b* values increased (<i>p</i> < 0.05). After d 30 of storage, the a* value increased in the samples with RP addition and this increase was higher in the with phosphate nuggets (<i>p</i> < 0.05).6. The internal surface a* value increased with the addition of RP during nugget production (<i>p</i> < 0.05). The increase in a* value was greater in samples with added phosphate (<i>p</i> < 0.05). During storage, the highest a* values were seen in nuggets treated with phosphate + 0.1% RP (<i>p</i> < 0.05). The addition of RP to chicken nugget emulsion improved redness, colour stability and shelf life.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"559-573"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of limestone particle size and microbial phytase on phosphorus and calcium digestion kinetics along the gastrointestinal tract in laying hens.","authors":"F Hervo, M-P Létourneau-Montminy, B Méda, A Narcy","doi":"10.1080/00071668.2024.2357659","DOIUrl":"10.1080/00071668.2024.2357659","url":null,"abstract":"<p><p>1. This study assessed the effect of limestone particle size and microbial phytase incorporation on the fate of phosphorus (P) and calcium (Ca) along the gastrointestinal tract in 72 laying hens.2. Four experimental diets were formulated according to a 2 × 2 factorial arrangement to evaluate the effect of two coarse limestone (CL) inclusion. This included a mix (MIX) of 75% CL (2 - 4 mm) and 25% fine particles (FL, <0.5 mm) or 100% FL, in two different basal diets formulated without (MIX0 and FL0) or with 300 FTU of microbial phytase/kg (MIX300 and FL300).3. Contents of the crop, gizzard, duodenum, jejunum and ileum were collected to determine the mean retention time (MRT) of dry matter (DM), the recovery rate of Ca and P in each segment of the gastrointestinal tract and the apparent fractional digestibility coefficient (AD) of Ca and P in each intestinal segment.4. In hens fed FL, microbial phytase decreased the MRT of DM along the intestine (<i>p</i> < 0.05). In the crop and the gizzard, Ca recovery increased with MIX incorporation to a greater extent in hens fed without microbial phytase (<i>p</i> < 0.05). The mixed particle size incorporation decreased absorption kinetics of Ca in hens fed microbial phytase. The AD of P and the absorption kinetics of P were significantly decreased in hens receiving FL300, probably due to complex formation between Ca and phytic acid.5. This study showed that coarse limestone particles incorporation improved mineral utilisation along the digestive tract.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"605-614"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141160780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food safety control in poultry industry: prevalence and antimicrobial susceptibility of <i>Escherichia coli</i> isolated from raw chicken and the potential use of <i>Origanum</i> essential oils as alternative to antibiotics.","authors":"C Abou-Jaoudeh, J Khalil, E El-Hayek, R Abou-Khalil","doi":"10.1080/00071668.2024.2346307","DOIUrl":"10.1080/00071668.2024.2346307","url":null,"abstract":"<p><p>1. The extensive use of antimicrobials in poultry production may contribute to the emergence of resistant bacteria. This study was conducted to determine the prevalence and resistance of different <i>E. coli</i> strains isolated from raw chicken meat and to investigate the possibility to use Lebanese native oregano essential oils as alternatives.2. In total, 250 chickens from Lebanese markets were examined for the presence of <i>E. coli</i>. Isolates were then screened for susceptibility using 19 antibiotics and two essential oils extracted from oregano plants.3. Of the 250 chickens tested, 80% were contaminated with <i>E. coli</i>. Main resistance was seen against amoxycillin, ampicillin, penicillin, tetracycline, tylosin, streptomycin and erythromycin. The highest rate of sensitivity was found in 86.1% of strains to Amoxycillin/Clavulanic acid, 80.09% to Tilmicosin. Both essential oils from <i>Origanum syriacum</i> (98%) and <i>O. ehrenbergii</i> (97.3%) showed promising potential in inhibiting the growth of the tested bacteria. Oil from <i>O. syriacum</i> exhibited superior efficacy against 200 <i>E. coli</i> strains, inhibiting 46.1% at 200 mg/l and all at 400 mg/l, while <i>O. ehrenbergii</i> oil showed slightly lower inhibition, affecting 41.6% at 200 mg/l and all at 400 mg/l.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"494-501"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimbiotic and wheat bran inclusion in maize or wheat-based diets for broiler chickens; effects on jejunal histomorphology, digesta oligosaccharides and caecal short-chain fatty-acids profiles.","authors":"S Veluri, G González-Ortiz, M R Bedford, O A Olukosi","doi":"10.1080/00071668.2024.2338535","DOIUrl":"10.1080/00071668.2024.2338535","url":null,"abstract":"<p><p>1. A stimbiotic (STB) is any feed additive that stimulates caeca fibre fermentation, although the additive itself contributes little to the caeca short-chain fatty acid (SCFA) production. A 42 d experiment investigated the interactive effects of STB and wheat bran (WB) in broiler chickens receiving maize or wheat-based diets.2. The treatments were arranged in a 2 × 2 × 2 factorial (eight replicates each), the dietary factors being diet (maize-SBM or wheat-SBM), STB (with or without) and WB (0 or 50 g/kg). Jejunal tissue, gizzard, jejunal and ileal digesta and caecal contents were collected on d 18 and 42.3. Gizzard pH tended to decrease with STB (<i>p</i> = 0.06) supplementation and was lower in birds fed wheat- compared to maize-based diets on d 18 (<i>p</i> < 0.05). Birds receiving diets with WB had higher jejunum pH on d 18 (<i>p</i> < 0.05).4. Total short-chain fatty acids (SCFA) in the caeca on d 18 and isobutyrate on d 42 were higher (<i>p</i> < 0.05) for maize compared with wheat-based diets. However, on d 42, acetate, butyrate and total SCFA were higher (<i>p</i> < 0.05) for wheat-based compared with maize-based diets.5. On d 18, STB and WB inclusion increased villi height (VH; <i>p</i> < 0.05) and VH to crypt depth ratio (VH/CD), respectively (<i>p</i> < 0.05). On d 42, VH (<i>p</i> < 0.05) and VH/CD were higher in wheat-based diets (<i>p</i> < 0.05). The VH/CD ratio was lower with STB supplementation (<i>p</i> < 0.05). Marker-corrected pentose oligosaccharides (Pent)₄ and (Pent)₅ concentrations in the ileal digesta were reduced (<i>p</i> < 0.05) with STB supplementation. In addition, STB decreased (Pent)₃ concentration in maize-, but not wheat-based diets (<i>p</i> < 0.05).6. In conclusion, both WB and STB influenced gastrointestinal pH and jejunum histomorphology of broilers without increasing oligosaccharide concentration in the ileum and SCFA in the caeca.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"465-477"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}