British Poultry SciencePub Date : 2026-08-01Epub Date: 2026-01-09DOI: 10.1080/00071668.2025.2602180
P Pootthachaya, W Puangsap, N Pintaphrom, T Srikha, U Sompong, A Cherdthong, B Yuangsoi, B Tengjaroensakul, I Hironori, S Wongtangtintharn
{"title":"Dietary polysaccharides extract from <i>Spirulina platensis</i>: a natural alternative for enhancing broiler performance and antioxidation status.","authors":"P Pootthachaya, W Puangsap, N Pintaphrom, T Srikha, U Sompong, A Cherdthong, B Yuangsoi, B Tengjaroensakul, I Hironori, S Wongtangtintharn","doi":"10.1080/00071668.2025.2602180","DOIUrl":"10.1080/00071668.2025.2602180","url":null,"abstract":"<p><p>1. The demand for natural alternatives to antibiotic growth promoters in poultry has led to growing interest in bioactive compounds, such as polysaccharides derived from algae. This study evaluated the effects of algae (<i>Spirulina platensis</i>)-derived polysaccharides (ADP) on growth performance, blood profiles, carcase characteristics, meat quality, amino acid composition and antioxidant status in broilers.2. A total of 320, 1-d-old Ross 308 mixed-sex broilers were randomly allocated to one of the five dietary treatments; a control diet (0% ADP) and diets supplemented with 0.15%, 0.30%, 0.45% and 0.60% of ADP. Each treatment included four replicates containing 16 birds each. The feeding trial was conducted over 42 d and was divided into three phases, including starter (1-14 d), grower (15-28 d) and finisher (29-42 d).3. Dietary inclusion of ADP at 0.30% significantly improved body weight gain and feed conversion ratio during the finisher (1.77 kg and 1.71, respectively) and overall (2.82 kg and 1.56, respectively) periods. There were no adverse effects observed on haematological, serum biochemistry or carcase characteristics.4. Supplementation with 0.45-0.60% ADP reduced cooking loss to 34-40% and improved meat cohesiveness by about 5%. Its inclusion elevated the levels of key amino acids, such as threonine, histidine, isoleucine, arginine and glutamic acid in the breast muscle. Additionally, ADP linearly increased hepatic antioxidant enzyme activities, including total superoxide dismutase (317.60-337.12 U/mg protein), glutathione peroxidase (39.98-48.70 U/mg protein), catalase (7.09-7.71 U/mg protein) and decreased malondialdehyde concentrations (0.63-0.68 nmol/mg protein) demonstrating enhanced oxidative status. These improvements were attributed to the prebiotic and antioxidant properties of <i>Spirulina</i>-derived polysaccharides, which enhance nutrient utilisation, maintain gut integrity and strengthen redox balance.5. This study provides preliminary evidence that polysaccharides derived from <i>S. platensis</i>, particularly at 0.30-0.45%, can be used as safe and effective feed additives in broiler diets. These compounds have the potential to enhance performance, oxidative stability and certain aspects of meat quality without adverse effects.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"662-675"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145932221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
British Poultry SciencePub Date : 2026-08-01Epub Date: 2026-01-19DOI: 10.1080/00071668.2025.2588245
T S Andrade, G N Comin, N Rohloff Junior, L I Datsch, G Toniazzo, N Borsatto, L F Ranna, D S Gust, P L O Carvalho, C Eyng, R V Nunes
{"title":"Alpha-amylase supplementation in reduced-energy diets enhances energy efficiency, mineral digestibility and intestinal health in broiler chickens.","authors":"T S Andrade, G N Comin, N Rohloff Junior, L I Datsch, G Toniazzo, N Borsatto, L F Ranna, D S Gust, P L O Carvalho, C Eyng, R V Nunes","doi":"10.1080/00071668.2025.2588245","DOIUrl":"10.1080/00071668.2025.2588245","url":null,"abstract":"<p><p>1. Limited starch digestibility in broiler diets, associated with granule structure and ingredient variability, can reduce energy utilisation and impair performance. Thus, this study aimed to evaluate the effects of alpha-amylase supplementation on performance, carcase yield, nutrient digestibility, intestinal morphology and short-chain fatty acid production.2. A total of 640 male broiler chickens (Cobb 500) were distributed in a completely randomised design with four treatments and eight repetitions of 20 birds each. The treatments: PC: positive control, formulated to meet the nutritional requirements of the birds; NC: negative control with a reduction of 0.25 MJ of metabolisable energy (ME) per kg of diet; NCA100: NC + 100 g alpha-amylase per kg of diet; NCA200; NC + 200 g alpha-amylase per kg of diet.3. At 21 and 42 d, performance was evaluated and, at 42 d, carcase yield and cuts, nutrient digestibility, intestinal morphology and short-chain fatty acid production were assessed in broiler chickens.4. At 21 and 42 d of age, broiler chickens supplemented with NCA200 exhibited improved metabolisable energy (ME) utilisation compared to the PC and NC groups. At 42 d, NCA200 supplementation increased ileal nutrient digestibility, as indicated by both digestible ash and the ash digestibility coefficient. At the same age, broiler chickens supplemented with NCA200 showed enhanced duodenal morphology, with greater villus height and villus-to-crypt ratio, as well as elevated caecal short-chain fatty acid concentrations, including acetic, propanoic and butyric acids, compared to the PC and NC groups.5. In conclusion, supplementation with NCA200 in reduced-energy diets increased mineral digestibility, promoted beneficial changes in intestinal morphology and elevated short-chain fatty acid production, demonstrating its efficacy in broiler diets without affecting performance.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"592-599"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
British Poultry SciencePub Date : 2026-08-01Epub Date: 2025-12-15DOI: 10.1080/00071668.2025.2593644
H Wall, M Boyner, E Wattrang, E Ivarsson
{"title":"Early access to feed and water and <i>Neurospora intermedia</i> in broiler starter diet: part I. production performance and organ development.","authors":"H Wall, M Boyner, E Wattrang, E Ivarsson","doi":"10.1080/00071668.2025.2593644","DOIUrl":"10.1080/00071668.2025.2593644","url":null,"abstract":"<p><p>1. This study evaluated the effects of early access to feed and water following hatch, and inclusion of <i>Neurospora intermedia</i> biomass (as a source of protein and bioactive compounds) in chicks' starter diet. Variables monitored were growth performance and organ development.2. A total of 432 Ross 308 chicks hatched on-farm were provided with either immediate access to feed and water (early-fed) or delayed access at 48 h post placement (late-fed). In addition, chicks were fed either a control diet (C) or a diet with 10% <i>N. intermedia</i> (N) until d 10, or fed N until d 6 followed by C (NC). From d 10 and onwards all chickens received the same commercial grower diet.3. Late-fed chicks had lower accumulated feed intake and BW until d 9. On d 5, the weights of all measured organs, except the bursa and spleen, were lower in late-fed chicks. At the same time point, late-fed chicks had longer intestines and lower heart weights, relative to body weight, compared to early-fed. None of these differences persisted at the end of study on d 43 when, instead, the bursa weights, both total and relative to body weight, were higher in late-fed chickens.4. During the initial period when only early-fed chicks had access to feed and water, diet C resulted in higher FI compared to diet NC, and a higher (inferior) FCR compared to dietary treatments N or NC. On d 43 relative bursa weights were higher in chickens fed diet N compared to C.5. The results suggested that chickens are capable of compensating for 48 h deprivation of feed and water post-hatch. Supplementing with fungal biomass of <i>N. intermedia</i> can partly replace soya protein concentrate as source of high-quality protein in diets for young chicks.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"608-614"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
British Poultry SciencePub Date : 2026-08-01Epub Date: 2026-01-02DOI: 10.1080/00071668.2025.2595642
H Wall, E Ivarsson, L Sun, M Boyner, M Naghizadeh, T S Dalgaard, E Wattrang
{"title":"Early access to feed, water and <i>Neurospora intermedia</i> in broiler starter diet (part II) - caecal microbiota, antibody production and blood leukocyte counts.","authors":"H Wall, E Ivarsson, L Sun, M Boyner, M Naghizadeh, T S Dalgaard, E Wattrang","doi":"10.1080/00071668.2025.2595642","DOIUrl":"10.1080/00071668.2025.2595642","url":null,"abstract":"<p><p>1. This study examined the effects of providing feed and water immediately post hatch in combination with inclusion of <i>Neurospora intermedia</i> biomass in chick starter diet, as a source of protein and bioactive compounds. Variables monitored were caecal microbiota, total levels of IgY, specific antibody responses to vaccination and blood leukocyte counts.2. Ross 308 chicks were subjected to two hatching treatments - immediate access to feed and water, or delayed access at 48 h. In addition, three starter diets were fed until d 10: a control diet (C), a diet with inclusion of 10% <i>N. intermedia</i> (N) or diet N followed by diet C (NC).3. Chicks were vaccinated against avian pneumovirus (APV) and focal birds were repeatedly blood sampled for quantification of total IgY and antibodies specific to APV in serum. Birds fed starter diet C were sampled for measures of blood leukocyte counts. Caecal microbiota were studied in birds sacrificed on d 5, 9 and 43, respectively.4. With age, microbial richness in caeca increased and there was a shift in composition, but no effects of hatching treatment or starter diet were observed.5. Neither the starter diet nor the hatching treatment affected the total IgY in serum or specific antibody responses to APV vaccination. Late-fed chicks had a significant decrease in the total amount of IgY in serum from d 2 to d 8, likely a consequence of dehydration at d 2. Early feeding did not affect any of the leukocyte populations monitored.6. In conclusion, there were no effects of early feeding or inclusion of <i>N. intermedia</i> on gut microbiome or the immune traits monitored. However, the absence of adverse effects of a starter diet with the fungal biomass suggested that <i>N. intermedia</i> is a source of high-quality protein.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"541-551"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145888222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
British Poultry SciencePub Date : 2026-08-01Epub Date: 2026-01-12DOI: 10.1080/00071668.2025.2605500
B Pan, Z Shi, L Ma, W Zhu, C Wang, J Bai, L Sun, J Cai
{"title":"Detection of salt content in pickled duck eggs based on X-ray imaging.","authors":"B Pan, Z Shi, L Ma, W Zhu, C Wang, J Bai, L Sun, J Cai","doi":"10.1080/00071668.2025.2605500","DOIUrl":"10.1080/00071668.2025.2605500","url":null,"abstract":"<p><p>1. To solve the problem of difficult real-time detection of salt content in the process of salted duck egg preservation, X-ray imaging technology (including digital radiography technology DR and computed tomography technology CT) was adopted to explore non-destructive detection methods for salt content in salted eggs.2. By analysing the differences in greyscale values of DR images of saltwater samples with different salinities, the feasibility of DR technology in quantitative detection of salt content in salted eggs was verified. The greyscale changes of DR under different preservation times were used to create a linear quantitative model of greyscale-salinity (Validation R2 = 0.96). This was established using image subtraction combined with CT technology allowing the spatial distribution characteristics of salt diffusion from albumen to yolk in salted eggs to be assessed.3. The results showed that X-ray imaging technology can realise efficient non-destructive detection of salt content in eggs, providing an important basis for optimising the high-pressure pulsating preservation process.4. This study improved the production efficiency and quality of salted eggs. It provided theoretical support and practical guidance for the technological innovation of non-destructive detection and the optimisation of preservation technology in the salted egg industry.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"562-570"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tibia density increases and keel bone damage accumulates gradually; a live laying hen longitudinal study with validated measurement of cortical and medullary bone.","authors":"P W Wilson, H A McCormack, A Raper, I C Dunn","doi":"10.1080/00071668.2026.2693059","DOIUrl":"https://doi.org/10.1080/00071668.2026.2693059","url":null,"abstract":"<p><p>1. Bone quality, particularly keel bone damage, has become an important aspect of laying hen welfare. This study investigated bone density changes in individual hens.2. Methods used included 2D live-bird X-ray to assess tibia and keel density longitudinally from 10 to 60 weeks of age. The data were used to estimate medullary and cortical bone components.3. Results showed that tibial bone density increased or remained stable after puberty, rather than declining during the laying cycle. This suggested that bone quality post-puberty requires reconsideration and the term 'osteoporosis' may need redefining.4. Tibial density increased significantly at puberty, primarily due to medullary bone accretion, and remained consistently high through the laying period. Keel bone damage occurred gradually over time, with no sudden increase. Hence, puberty can be crucial for establishing good bone quality and may provide a point where intervention can reduce keel bone damage in commercial flocks.5. The novel methodology of apportioning tibia area under the curve (AUC) for estimating cortical bone density from 2D X-ray demonstrated strong correlations with CT-derived density estimates. This reinforced its validity as a relatively simple way of assessing bone quality in live hens. The medullary bone correlation from 2D X-ray and CT-derived density was weaker, but total density was strongly correlated, so both can have application in understanding genetics, nutritional interventions or management.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Rashidi, A R Alizadeh Moghadam Masouleh, S D Sharifi, A Najafi
{"title":"Determination of digestible valine requirements for optimal growth of Japanese quail during the starter phase (1-21 days).","authors":"A Rashidi, A R Alizadeh Moghadam Masouleh, S D Sharifi, A Najafi","doi":"10.1080/00071668.2026.2675386","DOIUrl":"https://doi.org/10.1080/00071668.2026.2675386","url":null,"abstract":"<p><p>1. This study determined the requirement of digestible valine (dVal) and the optimal digestible valine-to-digestible lysine (dVal:dLys) ratio for Japanese quails during the starter phase (1-21 d).2. A total of 1000 one day old unsexed Japanese quail chicks (<i>Coturnix japonica</i>) were randomly assigned to five experimental diets: dVal (7.5, 8.5, 9.5, 10.5 or 11.5 g/kg diet), as five pen replicates each holding 40 birds.3. The optimal dietary dVal level for weight gain (WG), feed intake (FI), and feed conversion ratio (FCR) during d 1-21 were estimated using linear broken-line (LBL), quadratic broken-line (QBL), quadratic and logistic regression models to determine the optimal responses. Model evaluation was based on the coefficient of determination (R<sup>2</sup>), Akaike information criterion (AIC) and Bayesian information criterion (BIC).4. Chicks receiving a diet containing 0.95% dVal exhibited significantly higher WG (<i>p</i> = 0.0003) and improved FCR (<i>p</i> = 0.0330) compared to other treatments. The FI was unaffected by dVal levels over the 1-21 d period.5. Dose-response analysis using regression models revealed that the quadratic model provided the most accurate estimation of dVal requirements. The optimal dVal requirement for maximising WG and minimising FCR was determined to be 0.95% of the diet. Higher dVal levels (>9.5 g dVal/kg) were associated with increased FCR (<i>p</i> < 0.05). The estimated ideal dVal:dLys ratio to optimise performance in starter phase for Japanese quail was 0.79.6. The data showed that precise Val supplementation at recommended levels in low-protein diets can enhance growth in Japanese quail.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effects of walking with or without obstacles on broiler and duck growth and leg health.","authors":"K Trejo, G S Archer","doi":"10.1080/00071668.2026.2690659","DOIUrl":"https://doi.org/10.1080/00071668.2026.2690659","url":null,"abstract":"<p><p>1. Leg health is a persistent welfare concern in commercial poultry production. This study investigated whether increasing walking distance, alone or in combination with physical obstacles, could improve growth and leg health in broiler chickens and Pekin ducks.2. The experiment comprised four treatments applied from hatch to market weight: a short control pen (2 m long) with feed and water in close proximity (CON); a long pen (6 m long) with feed and water at opposite ends (WALK); a long pen with a step-over obstacle at the midpoint (OBS); and a long pen with a ramp at the midpoint (RAMP). Broiler chickens were reared to d 42 and Pekin ducks to d 35.3. Feed intake (FI), bird weight, feed conversion ratio (FCR), mortality, gait score and tibia ash and breaking strength were measured in both species.4. No differences (<i>p</i> > 0.05) were observed between treatments in mortality or tibia ash in either broilers or ducks. In broilers, CON birds consumed less feed (91.5 g/bird/d), weighed less at d 42 (2.50 kg), had higher gait scores (2.5) and lower tibia breaking strength (37.1 g) than all locomotion treatments (<i>p</i> < 0.05). No differences were found among the WALK, OBS or RAMP treatments (<i>p</i> > 0.05).5. In ducks, CON birds consumed more feed (125.5 g/bird/d), weighed less at d 35 (2.98 kg), had a higher FCR (1.80) and lower tibia breaking strength (27.0 g) than all locomotion treatments (<i>p</i> < 0.05). No differences were observed among the three locomotion treatments (<i>p</i> > 0.05).</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hen individual health traits and their association with keel bone damage.","authors":"J Marggraff, C Ahlers, K Donat, S Rautenschlein","doi":"10.1080/00071668.2026.2693064","DOIUrl":"https://doi.org/10.1080/00071668.2026.2693064","url":null,"abstract":"<p><p>1. Keel bone damage is a major welfare issue in laying hens. In this study, brown layers kept in commercial multi-tier systems were examined individually to gain information on individual health traits associated with keel bone deformations (KBD) and keel bone fractures (KBF).2. Ten laying hen flocks were assessed at six timepoints during the laying period for KBD, KBF and various health traits (<i>n</i> = 2900). On each visit, five hens with and three hens without keel bone damage were selected for further diagnostics (<i>n</i> = 462). This included X‑rays of the keel bone, <i>post-mortem</i> examination with histopathology (liver and intestines) and analysis of the keel bones (incineration) and tubular bones (breaking strength tests).3. Prevalence for KBD and KBF increased continuously with age (<i>p</i> < 0.0001) with highest mean prevalence at 65 weeks of age (KBD 47%, KBF 52%). Factors associated with KBD were presence of foot pad lesions (<i>p</i> = 0.0124) and lower dry matter (<i>p</i> = 0.0201) in dissected hens. Factors associated with KBF on-farm were higher body mass (<i>p</i> = 0.0239).4. After further diagnostics, KBF was associated with lower dry matter of the keel bone (<i>p</i> = 0.0049), lower relative calcium content of the keel bone (<i>p</i> = 0.0296), wing feather damage (<i>p</i> < 0.05) and enteritis (<i>p</i> = 0.0231). Overall, the occurrence of KBF was associated with KBD and vice versa (<i>p</i> < 0.0001). Palpated sharp bends in the keel on-farm were highly indicative for KBF (<i>p</i> < 0.0001).5. There might be a potential of reducing prevalence of KBF by stabilising gut health of laying hens and preventing foot and feather damage.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148444502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N S Akkaya, B Halaç, A I Kekeç, B Çelik, B Başaran Kahraman, B Diren Sığırcı, A F Bağcıgil, D Kesen, Y Çokal, S Ak, C Oz, M C Adıgüzel
{"title":"High-level Ciprofloxacin and Tigecycline resistance in <i>Salmonella enterica</i> sv. Molade from a slaughterhouse-based poultry sampling in the Turkish region: a cross-sectional study.","authors":"N S Akkaya, B Halaç, A I Kekeç, B Çelik, B Başaran Kahraman, B Diren Sığırcı, A F Bağcıgil, D Kesen, Y Çokal, S Ak, C Oz, M C Adıgüzel","doi":"10.1080/00071668.2026.2693063","DOIUrl":"10.1080/00071668.2026.2693063","url":null,"abstract":"<p><p>1. The aim of this study was to descriptively assess Ciprofloxacin and Tigecycline resistance in <i>Salmonella enterica</i> serovars isolated from poultry samples obtained from a limited number of farms and a single slaughterhouse in the Turkish region.2. A total of 550 samples were collected from slaughterhouses and farms. <i>Salmonella</i> spp. were isolated using the ISO 6579:2002/Amd 1:2007 culture method and presumptive isolates were confirmed using conventional biochemical tests, the BD Phoenix™ automated system and PCR targeting the <i>invA</i> gene.3. Selective media initially yielded 76 isolates with suspected <i>Salmonella</i> spp. morphology, of which 50 (9.1%) were confirmed as <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Molade (8, 20:z10:z6) according to the White-Kauffmann-Le Minor scheme.4. Antimicrobial susceptibility testing, interpreted according to EUCAST 2023 breakpoints, demonstrated resistance to Ciprofloxacin in all confirmed isolates (50/50, 100%) and resistance to Tigecycline in 44 isolates (88%). No isolates produced extended-spectrum β-lactamases, carbapenemases or AmpC β-lactamases and no multidrug-resistant profiles were detected.5. Given the restricted epidemiological scope of the study, these findings should be interpreted with caution. The observed resistance patterns in this rarely reported serovar suggest that further investigations involving broader, multi-site sampling are required to better elucidate its epidemiology and antimicrobial resistance characteristics.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-7"},"PeriodicalIF":1.7,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}