BioEssays最新文献_第3页

Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions 在宿主-病原体相互作用中可视化效应蛋白易位的实验方法。

IF 3.2 3区生物学

BioEssays Pub Date : 2025-03-13 DOI: 10.1002/bies.202400188

Verena Nadin Fritsch, Michael Hensel

{"title":"Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions","authors":"Verena Nadin Fritsch, Michael Hensel","doi":"10.1002/bies.202400188","DOIUrl":"10.1002/bies.202400188","url":null,"abstract":"<p>Bacterial pathogens deliver effector proteins into host cells by deploying sophisticated secretion systems. This effector translocation during host-pathogen interactions is a prerequisite for the manipulation of host cells and organisms and is important for pathogenesis. Analyses of dynamics and kinetics of translocation, subcellular localization, and cellular targets of effector proteins lead to understanding the mode of action and function of effector proteins in host-pathogen interplay. This review provides an overview of biochemical and genetic tools that have been developed to study protein effector translocation qualitatively or quantitatively. After introducing the challenges of analyses of effector translocation during host-pathogen interaction, we describe various methods ranging from static visualization in fixed cells to dynamic live-cell imaging of effector protein translocation. We show the main findings enabled by the approaches, emphasize the advantages and limitations of the methods, describe recent approaches that allow real-time tracking of effector proteins in living cells on a single molecule level, and highlight open questions in the field to be addressed by application of new methods.</p>","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"47 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.202400188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AAV Genome Topology Decides ITR Secondary Structure AAV 基因组拓扑决定 ITR 二级结构

IF 3.2 3区生物学

BioEssays Pub Date : 2025-03-05 DOI: 10.1002/bies.202400266

Patrick Wilmott, Leszek Lisowski

引用次数: 0

Phosphoregulation of Microtubule Assembly and Disassembly for Phragmoplast Expansion During Plant Cytokinesis 植物细胞质分裂过程中微管组装和拆卸的磷酸化调控。

IF 3.2 3区生物学

BioEssays Pub Date : 2025-03-03 DOI: 10.1002/bies.202500004

Yuh-Ru Julie Lee, Bo Liu

{"title":"Phosphoregulation of Microtubule Assembly and Disassembly for Phragmoplast Expansion During Plant Cytokinesis","authors":"Yuh-Ru Julie Lee, Bo Liu","doi":"10.1002/bies.202500004","DOIUrl":"10.1002/bies.202500004","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant cytokinesis results in the formation of the cell plate by the phragmoplast which contains dynamic microtubules serving as the track for the delivery of cell wall builders included in Golgi vesicles. During the centrifugal process of cell plate assembly, new microtubules are assembled and bundled at the leading edge to prepare for vesicle transport while older microtubules are disassembled at the lagging edge upon the completion of vesicle delivery. The turnover of phragmoplast microtubules in this process is thought to be regulated by phosphorylation of the key microtubule bundling factor MAP65. A recent study revealed a surprising role of the <i>α</i>-Aurora kinase, which is typically known for its role in governing the formation of the bipolar spindle apparatus, in phosphorylating the primary microtubule bundler MAP65-3 in Arabidopsis. This phosphorylation positively contributes to the expansion of the phragmoplast. The phragmoplast midzone is also the hub for other cytokinesis-important kinases. It is intriguing how these kinases are targeted and how they may crosstalk with each other to orchestrate the expansion of the phragmoplast.</p>\u0000 </div>","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"47 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Toxicants and Their Disruption of Integrin Signaling in Lipid Rafts 脂筏中整合素信号的环境及其干扰。

IF 3.2 3区生物学

BioEssays Pub Date : 2025-02-26 DOI: 10.1002/bies.202400276

Tina Izard

{"title":"Environmental Toxicants and Their Disruption of Integrin Signaling in Lipid Rafts","authors":"Tina Izard","doi":"10.1002/bies.202400276","DOIUrl":"10.1002/bies.202400276","url":null,"abstract":"<div>\u0000 \u0000 <p>Talin, a key integrin activator, is essential for cellular adhesion, signal transduction, and mechanical stability. Its transition between autoinhibited and active conformations allows dynamic regulation of adhesion in response to environmental cues. Cholesterol-rich membrane microdomains, such as lipid rafts, organize and stabilize signaling platforms, influencing talin and integrin conformational states. Cholesterol is a switch modulating talin activation, integrin binding, and adhesion. Environmental pollutants, including heavy metals and air toxins, disrupt cholesterol homeostasis, destabilize lipid rafts, and interfere with talin–integrin interactions. These disruptions impair adhesion, tissue repair, and signaling fidelity, contributing to atherosclerosis and cancer metastasis. Understanding talin's interaction with cholesterol-rich domains offers critical insights into adhesion regulation and reveals the broader impact of environmental toxicants on cellular function. This framework emphasizes the importance of membrane composition, particularly cholesterol, in mediating the effects of environmental stressors and suggests potential therapeutic interventions.</p>\u0000 </div>","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"47 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The LAM Is Not Enough–An Idea to Watch Regarding Adipose Tissue Macrophages and Their Disease Relevance LAM是不够的-一个关于脂肪组织巨噬细胞及其疾病相关性的观点：为什么脂质相关巨噬细胞（LAM）在脂肪组织中的积累是一个系统生物学问题。

IF 3.2 3区生物学

BioEssays Pub Date : 2025-02-26 DOI: 10.1002/bies.202500020

Lorenz Adlung

引用次数: 0

Gene Duplication and Alternative Splicing as Evolutionary Drivers of Proteome Specialization 基因复制和选择性剪接是蛋白质组特化的进化驱动因素。

IF 3.2 3区生物学

BioEssays Pub Date : 2025-02-25 DOI: 10.1002/bies.202400202

Federica Mantica, Manuel Irimia

引用次数: 0

Issue Information: BioEssays 3/2025 期刊信息：BioEssays 3/2025

IF 3.2 3区生物学

BioEssays Pub Date : 2025-02-23 DOI: 10.1002/bies.202570003

引用次数: 0

Evolutionary Origins and Adaptive Significance of A-to-I RNA Editing in Animals and Fungi A-to-I RNA编辑在动物和真菌中的进化起源和适应性意义。

IF 3.2 3区生物学

BioEssays Pub Date : 2025-02-21 DOI: 10.1002/bies.202400220

Yanfei Du, Chenhui Wang, Yu Zhang, Huiquan Liu

{"title":"Evolutionary Origins and Adaptive Significance of A-to-I RNA Editing in Animals and Fungi","authors":"Yanfei Du, Chenhui Wang, Yu Zhang, Huiquan Liu","doi":"10.1002/bies.202400220","DOIUrl":"10.1002/bies.202400220","url":null,"abstract":"<div>\u0000 \u0000 <p>Adenosine-to-inosine (A-to-I) RNA editing, capable of protein recoding, has evolved independently in animals and fungi. This study proposes adaptive hypotheses regarding its origins and phenotypic significance, suggesting that A-to-I editing enhances adaptability by alleviating genetic trade-offs. In metazoans, its emergence may have been driven by a development-defense trade-off associated with transposable element activation during the evolution of multicellularity. Late Devonian cooling and End-Permian warming are hypothesized to have driven the emergence of extensive A-to-I recoding in coleoid nervous systems and Sordariomycete sexual fruiting bodies, respectively. These adaptations may have influenced key evolutionary innovations, including the evolution of metazoan nervous systems, coleoid intelligence, and shell loss, and fungal sexual reproductive structures. Additionally, extensive A-to-I recoding is proposed to facilitate accelerated development and specific life-history strategies in both animals and fungi. This paper provides new perspectives on the evolutionary forces shaping A-to-I RNA editing and its role in phenotypic diversity across taxa.</p>\u0000 </div>","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"47 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor-Associated Macrophages Write the Script of Cancer Obesity Paradox 肿瘤相关巨噬细胞书写癌症肥胖悖论的脚本。

IF 3.2 3区生物学

BioEssays Pub Date : 2025-02-19 DOI: 10.1002/bies.202400264

Anshul Sharma, Alok K. Mishra

引用次数: 0

Intron Retention, an Orchestrated Program of Gene Expression Regulation 内含子保留，基因表达调控的一个精心安排的程序。

IF 3.2 3区生物学

BioEssays Pub Date : 2025-02-14 DOI: 10.1002/bies.202400248

Hua Zhou, Xing Wang Deng

引用次数: 0