BioEssays最新文献_第3页

The Role of Arp2/3 in End-Resection During DNA Double-Strand Break Repair Arp2/3在DNA双链断裂修复中末端切除中的作用。

IF 3.3 3区生物学

BioEssays Pub Date : 2026-09-09 DOI: 10.1002/bies.70171

Felix Y. Zhou, James E. Haber

{"title":"The Role of Arp2/3 in End-Resection During DNA Double-Strand Break Repair","authors":"Felix Y. Zhou, James E. Haber","doi":"10.1002/bies.70171","DOIUrl":"10.1002/bies.70171","url":null,"abstract":"The efficient repair of chromosome breaks by homologous recombination depends on the ability of the ends of a double-strand break (DSB) to locate homologous sequences to serve as templates to repair the lesion. These repair mechanisms are conserved across eukaryotic evolution. Repair depends on 5’ to 3’ resection of the broken ends, allowing the assembly of a Rad51 filament. In yeast, fruit flies, and mammals, broken chromosome ends display increased mobility, allowing the ends to explore a larger volume. The Arp2/3 actin nucleator complex plays a key role in increased local chromatin mobility near a DSB. In budding yeast, Arp2/3, LAS17WASP, and myosins are required both to initiate and to maintain long-range end resection, affecting both DNA repair and the maintenance of the DNA damage checkpoint. At least in budding yeast, the connection between controlling long-range resection and increased mobility is direct, as inhibiting resection prevents damage-induced chromatin mobility. Moreover, although the search for homology by Rad51-coated broken DNA ends would seem to be improved by greater exploration of the nuclear volume, increased local mobility through Arp2/3 per se does not affect the efficiency of ectopic recombination in yeast. Here, we review the recently defined roles of nuclear actin in DNA damage repair, with a focus on new observations in budding yeast.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"48 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.70171","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Roads of CENP-A for Centromere Identification CENP-A在着丝粒识别中的双重途径。

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-24 DOI: 10.1002/bies.70177

Tetsuya Hori, Tatsuo Fukagawa

{"title":"Dual Roads of CENP-A for Centromere Identification","authors":"Tetsuya Hori, Tatsuo Fukagawa","doi":"10.1002/bies.70177","DOIUrl":"10.1002/bies.70177","url":null,"abstract":"Faithful chromosome segregation requires the kinetochore, a macromolecular protein complex that assembles on centromeric chromatin. In vertebrates, centromere identity is epigenetically defined by the histone H3 variant CENP-A, whose nucleosomes are replenished during early G1 phase through a cell cycle-regulated deposition mechanism. The CENP-A chaperone HJURP is recruited to centromeres via the Mis18 complex to enable CENP-A incorporation (Mis18C pathway). Recent genetic analyses in chicken cells, however, revealed an additional, independent recruitment route via direct HJURP–CENP-C interaction (CENP-C pathway). Structural studies show that CENP-C and the Mis18C subunit KNL2/M18BP1 engage pre-existing CENP-A nucleosomes at different surfaces of the Constitutive Centromere-Associated Network (CCAN), constraining the spatial coordinates where new CENP-A is incorporated. This review summarizes current understanding of HJURP recruitment mechanisms for vertebrate CENP-A deposition and proposes that this structure-defined deposition geometry underlies epigenetic self-propagation of centromere positional information by maintaining proper CCAN spacing across cell divisions.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"48 8","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Binary: Cardiac Patterning as Probabilistic Fate Restriction 超越二元：作为概率命运限制的心脏模式

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-19 DOI: 10.1002/bies.70163

Kenzo Ivanovitch

{"title":"Beyond Binary: Cardiac Patterning as Probabilistic Fate Restriction","authors":"Kenzo Ivanovitch","doi":"10.1002/bies.70163","DOIUrl":"https://doi.org/10.1002/bies.70163","url":null,"abstract":"The mammalian heart is classically described as arising from two cardiac lineages, first and second. Yet, recent lineage tracing of Mesp1 mesoderm and live imaging show that cardiac progenitors are already partly biased toward specific heart regions during gastrulation, despite extensive cell mixing. We propose that cardiac fate is probabilistic, analogous to stochastic fate strategies in the retina. Epiblast cells carry probability distributions over fates that resolve during gastrulation into specific regional allocations. Reanalyzing a retrospective clonal dataset, we find that the clones do not single out one fixed lineage tree. Instead, the data permit a family of 361 distinct restriction topologies, none dominant, and only 40 of these are binary. Among the binary trees, the best-supported recover both where progenitors sit along the proximal–distal axis of the streak and the order in which they leave it, as recent prospective lineage tracing shows. Rather than replacing the first/second lineage concept, this framework shifts the focus from fixed lineage identity to fate probabilities. Testing this idea will require prospective live imaging from epiblast through heart tube formation.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"48 8","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.70163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Binary: Cardiac Patterning as Probabilistic Fate Restriction 超越二元：作为概率命运限制的心脏模式

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-19 DOI: 10.1002/bies.70163

Kenzo Ivanovitch

{"title":"Beyond Binary: Cardiac Patterning as Probabilistic Fate Restriction","authors":"Kenzo Ivanovitch","doi":"10.1002/bies.70163","DOIUrl":"https://doi.org/10.1002/bies.70163","url":null,"abstract":"The mammalian heart is classically described as arising from two cardiac lineages, first and second. Yet, recent lineage tracing of Mesp1 mesoderm and live imaging show that cardiac progenitors are already partly biased toward specific heart regions during gastrulation, despite extensive cell mixing. We propose that cardiac fate is probabilistic, analogous to stochastic fate strategies in the retina. Epiblast cells carry probability distributions over fates that resolve during gastrulation into specific regional allocations. Reanalyzing a retrospective clonal dataset, we find that the clones do not single out one fixed lineage tree. Instead, the data permit a family of 361 distinct restriction topologies, none dominant, and only 40 of these are binary. Among the binary trees, the best-supported recover both where progenitors sit along the proximal–distal axis of the streak and the order in which they leave it, as recent prospective lineage tracing shows. Rather than replacing the first/second lineage concept, this framework shifts the focus from fixed lineage identity to fate probabilities. Testing this idea will require prospective live imaging from epiblast through heart tube formation.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"48 8","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.70163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Dynamic Alliance of p53 and Metabolism in the Tumor Microenvironment Shapes Tumor Evolution 肿瘤微环境中p53与代谢的动态联盟影响肿瘤进化

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-19 DOI: 10.1002/bies.70175

Sebastien M. Joruiz, Francesco Napoletano, Rebecca Bertolio, Giannino Del Sal

{"title":"The Dynamic Alliance of p53 and Metabolism in the Tumor Microenvironment Shapes Tumor Evolution","authors":"Sebastien M. Joruiz, Francesco Napoletano, Rebecca Bertolio, Giannino Del Sal","doi":"10.1002/bies.70175","DOIUrl":"https://doi.org/10.1002/bies.70175","url":null,"abstract":"Tumor evolution, from premalignant lesions to metastasis, is increasingly recognized as shaped by continuous interplay between tumor cells metabolism and their microenvironment. During tumor initiation, major oncogenic pathways drive early metabolic reprogramming of lipid, amino acid, and energy pathways to promote cell competition and clonal expansion. These metabolic changes reciprocally shape the tumor microenvironment (TME) through metabolite fluxes, extracellular matrix remodeling, and immune reprogramming, generating adaptive niches that sustain tumor progression and metastasis. Cancer cell metabolic adaptability becomes even more crucial to survive dissemination and adapt to a new, distant microenvironment.Here, we discuss these dynamic interplays and highlight the p53 pathway as an integrative hub linking oncogenic signaling, metabolic rewiring, and tumor microenvironmental adaptation throughout carcinogenesis. We will also outline how emerging technologies may redefine the TME-p53-metabolism interplay uncovering therapeutically exploitable metabolic vulnerabilities.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"48 8","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.70175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Dynamic Alliance of p53 and Metabolism in the Tumor Microenvironment Shapes Tumor Evolution 肿瘤微环境中p53与代谢的动态联盟影响肿瘤进化

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-19 DOI: 10.1002/bies.70175

Sebastien M. Joruiz, Francesco Napoletano, Rebecca Bertolio, Giannino Del Sal

{"title":"The Dynamic Alliance of p53 and Metabolism in the Tumor Microenvironment Shapes Tumor Evolution","authors":"Sebastien M. Joruiz, Francesco Napoletano, Rebecca Bertolio, Giannino Del Sal","doi":"10.1002/bies.70175","DOIUrl":"https://doi.org/10.1002/bies.70175","url":null,"abstract":"Tumor evolution, from premalignant lesions to metastasis, is increasingly recognized as shaped by continuous interplay between tumor cells metabolism and their microenvironment. During tumor initiation, major oncogenic pathways drive early metabolic reprogramming of lipid, amino acid, and energy pathways to promote cell competition and clonal expansion. These metabolic changes reciprocally shape the tumor microenvironment (TME) through metabolite fluxes, extracellular matrix remodeling, and immune reprogramming, generating adaptive niches that sustain tumor progression and metastasis. Cancer cell metabolic adaptability becomes even more crucial to survive dissemination and adapt to a new, distant microenvironment.Here, we discuss these dynamic interplays and highlight the p53 pathway as an integrative hub linking oncogenic signaling, metabolic rewiring, and tumor microenvironmental adaptation throughout carcinogenesis. We will also outline how emerging technologies may redefine the TME-p53-metabolism interplay uncovering therapeutically exploitable metabolic vulnerabilities.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"48 8","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.70175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying Evolutionary Dynamics of Arthropodization and Arthrodization 量化节肢化和节肢化的进化动力学。

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-13 DOI: 10.1002/bies.70174

Cong Liu, Yu Wu, Dongjing Fu, Xingliang Zhang

{"title":"Quantifying Evolutionary Dynamics of Arthropodization and Arthrodization","authors":"Cong Liu, Yu Wu, Dongjing Fu, Xingliang Zhang","doi":"10.1002/bies.70174","DOIUrl":"10.1002/bies.70174","url":null,"abstract":"<div>\u0000 \u0000 Euarthropods are characterized by segmented, sclerotized, and articulated bodies and appendages, expressed through arthrodization and arthropodization. Both are regarded as defining features of Euarthropoda and have been proposed to evolve in a specific sequence, during which arthropodization preceded arthrodization, and initiated in frontal appendages and was subsequently co-opted into other appendages. Here, we investigate their evolution across Ecdysozoa using morphological comparison, ancestral-state reconstruction (ASR), and semi-quantitative methods. Morphological comparisons and ASR reveal repeated gains, reductions, losses, and regional decoupling of segmentation, sclerotization, and articulation in both body and appendicular systems. Semi-quantitative indices indicate variable degrees of arthrodization and arthropodization across ecdysozoan lineages and do not show a progressive increase from the basal panarthropods and the stem-group euarthropods to crown-group euarthropods. Together, these results support a modular, convergent evolution of arthrodization and arthropodization in different lineages, probably through repeated modification, reduction, or redeployment of the ancestral developmental toolkit.\u0000 </div>","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"48 8","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Single-Stranded DNA Platform: A Potential Broad-Spectrum Vector for Editing Genes in Prokaryotes 单链DNA平台：原核生物基因编辑的潜在广谱载体：ssDNA可能突破非模式细菌基因操作的困难。

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-13 DOI: 10.1002/bies.70172

Huayi Wang, Yefeng Wang, Dingkun Chang, Jie Fang, Zixuan Zheng, Xiangfeng Wang, Hongju Yang, Zhi Liu

引用次数: 0

Mechanisms That Govern Recombinase Fidelity Control During Eukaryotic Homologous Recombination 真核生物同源重组过程中控制重组酶保真度的机制。

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-13 DOI: 10.1002/bies.70173

Ilayda Korkmaz, J. Brooks Crickard

引用次数: 0

Flower Delivery: The Emergence of FLOWER (FWE) as a Multifunctional Regulator of Endo-Lysosome-Related Organelle Trafficking Across Cell Types 花传递：花（FWE）作为内切溶酶体相关细胞器跨细胞运输的多功能调节剂的出现。

IF 3.3 3区生物学

BioEssays Pub Date : 2026-08-13 DOI: 10.1002/bies.70167

Justin C. Rudd, Laura A. Hansen

{"title":"Flower Delivery: The Emergence of FLOWER (FWE) as a Multifunctional Regulator of Endo-Lysosome-Related Organelle Trafficking Across Cell Types","authors":"Justin C. Rudd, Laura A. Hansen","doi":"10.1002/bies.70167","DOIUrl":"10.1002/bies.70167","url":null,"abstract":"In humans, the FLOWER (FWE or CACFD1) gene encodes a set of small, alternatively spliced transmembrane protein isoforms (hFWE1-4). The canonical human isoform, hFWE4, and its various orthologues, which we collectively refer to as FWE for simplicity, assumes a four transmembrane pass structure with cytosolic N- and C-termini. This topology exposes YXXΦ motifs enabling interaction with trafficking machinery and positions highly-conserved cationic residues for interaction with membrane phospholipids. While identified initially as a mediator of synaptic vesicle endocytosis in presynaptic neurons, FWE is now recognized as a critical mediator of endo-lysosome related organelle (ELRO) trafficking across diverse cell types including cytotoxic T-lymphocytes and epidermal keratinocytes. A unifying feature of FWE function appears to be elevation of cytosolic Ca2+ levels. However, the underlying molecular mechanisms remain elusive. This review synthesizes current literature on FWE-mediated membrane trafficking, highlighting the evolutionary conservation of its coding sequence and tertiary structure. We propose new structure-compatible hypotheses for FWE-dependent cytosolic Ca2+ elevation and subsequent ELRO trafficking, highlighting experimental strategies that may aid testing of these hypotheses. Finally, we examine the distinct localizations and potential functions of non-canonical FWE isoforms.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":"48 8","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.70167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148722052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0