BioEssays最新文献_第2页

Biosensors for Detecting Small Rho GTPases: Monitoring Expression and Activation. 用于检测小Rho gtpase的生物传感器：监测表达和激活。

IF 2.7 3区生物学

BioEssays Pub Date : 2025-09-10 DOI: 10.1002/bies.70069

Nik Yasmin Umaira Hasnizan, Chong Chien Fung, Saw Keat Chuan, Nurul Syafawani Fizal, Noorhayati Idros, Ana Masara Ahmad Mokhtar

{"title":"Biosensors for Detecting Small Rho GTPases: Monitoring Expression and Activation.","authors":"Nik Yasmin Umaira Hasnizan, Chong Chien Fung, Saw Keat Chuan, Nurul Syafawani Fizal, Noorhayati Idros, Ana Masara Ahmad Mokhtar","doi":"10.1002/bies.70069","DOIUrl":"https://doi.org/10.1002/bies.70069","url":null,"abstract":"Advanced biosensing technologies, such as Förster resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET), have enabled real-time, high-resolution tracking of Rho GTPase activity, surpassing traditional methods like pull-down assays. However, current biosensors mainly detect the GTP-bound active state through effector interactions, without directly measuring Rho GTPase expression or identifying related biomarkers of abnormal activation. Small Rho GTPases are essential molecular switches that regulate key cellular processes such as cytoskeletal organization, cell movement, polarity, vesicle trafficking, and the cell cycle. Their precise activation and deactivation are critical for cellular balance, and disruptions in their signaling pathways are linked to diseases like cancer and immune disorders. Monitoring their activity is vital for understanding these processes and developing treatments. This study highlights the need for next-generation biosensors capable of directly monitoring expression levels and novel biomarkers, offering new avenues for research and therapeutic development targeting small Rho GTPases.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":" ","pages":"e70069"},"PeriodicalIF":2.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Perspectives on Gonadotropin Regulation in Vertebrates Revealed by the Discovery of FSH-RH in Teleosts. 硬骨鱼中FSH-RH的发现揭示了脊椎动物促性腺激素调节的新观点。

IF 2.7 3区生物学

BioEssays Pub Date : 2025-09-10 DOI: 10.1002/bies.70066

Daichi Kayo, Shun Kenny Uehara, Muhammad Rahmad Royan, Shinji Kanda

引用次数: 0

Fusion Pores as Regulators of Quantal Size and Cellular Physiology. 融合孔作为量子大小和细胞生理的调节因子。

IF 2.7 3区生物学

BioEssays Pub Date : 2025-09-07 DOI: 10.1002/bies.70064

Bhavya R Bhaskar, Shahina Mazumdar, Sruthilaya Dayanandan, Debasis Das

引用次数: 0

Shear Stress as a Danger Signal: Inducing Inflammation and Thrombosis via Mechanosensitive NETosis. 剪切应力作为危险信号：通过机械敏感性NETosis诱导炎症和血栓形成。

IF 2.7 3区生物学

BioEssays Pub Date : 2025-09-04 DOI: 10.1002/bies.70065

Sara Baratchi, Karlheinz Peter

{"title":"Shear Stress as a Danger Signal: Inducing Inflammation and Thrombosis via Mechanosensitive NETosis.","authors":"Sara Baratchi, Karlheinz Peter","doi":"10.1002/bies.70065","DOIUrl":"https://doi.org/10.1002/bies.70065","url":null,"abstract":"Neutrophil extracellular traps (NETs)-web-like DNA structures extruded by neutrophils in response to various stimuli, including pathogens, sterile inflammation, and mechanical stress-play a dual role in immunity and disease. While NETs serve to trap and neutralize pathogens during host defense, excessive or dysregulated NET formation, known as NETosis, can amplify inflammation and contribute to thrombotic complications such as atherosclerosis and valve disease. Increasing evidence supports that NETosis is a regulated, signaling-driven process, and that mechanical forces-including shear stress, tensile force, and matrix stiffness-can act as noncanonical danger signals capable of inducing NETosis. Mechanosensitive ion channels such as Piezo1, have emerged as key transducers of these biophysical cues, enabling cells to convert changes in shear stress levels into intracellular calcium flux, cytoskeletal remodeling, and ultimately NET release. Furthermore, exposure to pathologically high levels of shear stress may improve the sensitivity of neutrophils to secondary stimuli, lowering their activation threshold and amplifying inflammatory and thrombotic cascades. This mechanosensitive framework highlights shear-induced NETosis as a critical pathway by which neutrophils contribute to inflammation and thrombosis in mechanically stressed vascular environments.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":" ","pages":"e70065"},"PeriodicalIF":2.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conformational Rearrangement of Fission DSPs. 裂变dsp的构象重排。

IF 2.7 3区生物学

BioEssays Pub Date : 2025-09-01 DOI: 10.1002/bies.70062

Anelise N Hutson, Kristy Rochon, Jason A Mears

{"title":"Conformational Rearrangement of Fission DSPs.","authors":"Anelise N Hutson, Kristy Rochon, Jason A Mears","doi":"10.1002/bies.70062","DOIUrl":"https://doi.org/10.1002/bies.70062","url":null,"abstract":"Dynamin superfamily proteins (DSPs) are large GTPases that play crucial roles in membrane remodeling processes, including vesicle uptake, mitochondrial fission, and opposing fusion events. Among them, dynamin and dynamin-related protein 1 (Drp1) share a conserved domain architecture, yet exhibit unique structural and regulatory features that tailor their functions. This review explores the conformational rearrangements of the mammalian fission DSPs, dynamin and Drp1, focusing on their dimeric and tetrameric structures, lipid-bound assemblies, and key regulatory elements that drive membrane constriction. Structural biology methods, including x-ray crystallography and cryo-electron microscopy, have provided insight into the mechanism of activation and constriction of these DSPs, revealing how domain interactions and intrinsically disordered regions regulate self-assembly and enzymatic activity. We briefly examine the role of sequence modifications and partner proteins in modulating DSP function, highlighting the impact of regulatory factors on their respective cellular functions. An ongoing goal is to better understand the molecular mechanisms governing the transitions from a pre-assembled cytosolic state to a self-assembled state for dynamin and Drp1 on membranes, which provides a foundation for studying subsequent helical constriction. This insight will enhance our knowledge of organelle dynamics and provide new avenues for therapeutic interventions targeting DSP-related pathologies.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":" ","pages":"e70062"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taking Down the Primary Cilium: Pathways for Disassembly in Differentiating Cells. 初级纤毛的脱落：分化细胞的拆卸途径。

IF 2.7 3区生物学

BioEssays Pub Date : 2025-09-01 DOI: 10.1002/bies.70060

Carolyn M Ott, Saikat Mukhopadhyay

引用次数: 0

When the Clock Is Ticking: The Role of Mitotic Duration in Cell Fate Determination. 当时钟滴答作响：有丝分裂持续时间在细胞命运决定中的作用。

IF 2.7 3区生物学

BioEssays Pub Date : 2025-08-30 DOI: 10.1002/bies.70061

Cornelia Sala, Elmar Schiebel

{"title":"When the Clock Is Ticking: The Role of Mitotic Duration in Cell Fate Determination.","authors":"Cornelia Sala, Elmar Schiebel","doi":"10.1002/bies.70061","DOIUrl":"https://doi.org/10.1002/bies.70061","url":null,"abstract":"Mitosis is a crucial phase of the cell cycle, during which several mechanisms work together to ensure accurate chromosome segregation and to eliminate defective cells if errors occur. One key mechanism is the spindle assembly checkpoint (SAC), which upon mitotic errors-such as those induced by genetic mutations, drug treatments, or environmental stresses-arrest cells in mitosis. Arrested cells may undergo apoptosis during mitosis or eventually exit mitosis even if the damage remains unrepaired. Mitotic exit is driven by a reduction in cyclin B1 levels, regulated during mitosis by multiple mechanisms affecting both its synthesis and degradation. Strikingly, cells harboring the tumor suppressor p53 can monitor the duration of mitosis and encode this information as a form of \"mitotic memory\". This memory influences the fate of daughter cells after mitotic exit by inducing G1 arrest through p53-dependent expression of the cyclin-dependent kinase (CDK) inhibitor p21. Recent studies have proposed mechanisms by which cyclin B1 levels are regulated during mitotic arrest and how p53 promotes mitotic-arrest-dependent transcription of p21 in G1. These findings indicate that both the expression of regulators that control mitotic duration and the activity of proteins that monitor the duration of mitosis and halt proliferation work together to determine cell fate following mitotic errors. Understanding these mechanisms offers valuable insights for cancer therapy, particularly regarding the strategic application of antimitotic agents.","PeriodicalId":9264,"journal":{"name":"BioEssays","volume":" ","pages":"e70061"},"PeriodicalIF":2.7,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BioEssays 9/2025 BioEssays 9/2025

IF 2.7 3区生物学

BioEssays Pub Date : 2025-08-25 DOI: 10.1002/bies.70053