BMC Microbiology最新文献

{"title":"Fecal microbiota transplantation mitigates postdieting weight regain in mice by modulating the gut-liver axis.","authors":"Hong Cao, Jiangwei Xu, Han Wang, Wanya Yi, Dandan Yang, Ju Yang, Jing Sun, Yingyu Wang, Feng Zhang, Jiai Yan, Dan Li","doi":"10.1186/s12866-025-03853-4","DOIUrl":"10.1186/s12866-025-03853-4","url":null,"abstract":"<p><strong>Background: </strong>Dysbiosis of the microbiome is strongly associated with weight rebound after dieting. However, the interactions between the host and microbiome and their relevance to the pathogenesis of post-diet weight rebound remain unclear.</p><p><strong>Purpose: </strong>This study aimed to evaluate the effects of fecal microbiota transplantation (FMT) on post-diet weight regain and to investigate the underlying mechanisms by which FMT inhibits weight regain.</p><p><strong>Methods: </strong>FMT was administered once daily to mice for 5 weeks. Gas chromatography tandem mass spectrometry was employed to analyze short-chain fatty acid levels in serum, ultrahigh-performance liquid chromatography tandem mass spectrometry was utilized for analyzing hepatic lipid metabolites, and shotgun metagenomic sequencing was applied to examine the intestinal microbiome.</p><p><strong>Results: </strong>FMT reduced weight regain and prevented lipid accumulation in both liver and adipose tissue while also improving glucose intolerance in mice. Furthermore, FMT increased the abundance of Enterorhabdus caecimuris and decreased the abundances of Burkholderiales, Sutterellaceae, Turicimonas muris, Bacteroides stercorirosoris, and Acetivibrio ethanolgignens within the gut microbiota. Additionally, elevated propionic acid levels and significant alterations in hepatic lipid metabolites were observed following FMT administration.</p><p><strong>Conclusions: </strong>Our findings demonstrate that FMT effectively mitigates post-diet weight regain and associated complications. These effects are mediated through interactions between the gut microbiota and the liver via the gut-propionic acid-liver axis.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"135"},"PeriodicalIF":4.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early prediction of invasive fungal infection risk in acute-on-chronic liver failure: a prediction model based on admission indicators.","authors":"Xu Yang, Jie Li, Yanli Yang, Li Zhang, Xuelian Dan, Dachuan Cai, Zhi Zhou, Hu Li, Xiaohao Wang, Shan Zhong","doi":"10.1186/s12866-025-03819-6","DOIUrl":"10.1186/s12866-025-03819-6","url":null,"abstract":"<p><strong>Background: </strong>Acute-on-chronic liver failure (ACLF) is a severe clinical syndrome, and the incidence of invasive fungal infection (IFI) among hospitalized patients with ACLF is steadily increasing. The aim of this study is to develop a diagnostic nomogram to assist in the identification of IFI in these patients.</p><p><strong>Methods: </strong>A retrospective study included 705 patients from January 1, 2019, to October 31, 2023, randomly divided into training (n = 493) and validation (n = 212) cohorts. The diagnosis of IFI includes proven diagnosis and probable diagnosis. Kaplan analysis was performed to analyze the survival prognosis of ACLF patients with and without IFI. A nomogram was developed based on a logistic regression model derived through least absolute shrinkage and selection operator (LASSO) regression. The discrimination, accuracy, and clinical utility of the model were assessed using receiver operating characteristic curves, Hosmer-Lemeshow tests, calibration plots, and decision curve analysis.</p><p><strong>Results: </strong>Kaplan-Meier survival analysis confirmed that the median survival time of ACLF patients with IFI was significantly lower (by 68 days) than that of ACLF patients without IFI, and there were significant differences in the 90-day, 180-day, and 360-day survival rates between the two groups (P < 0.05). Based on LASSO regression, the following factors were identified as significant risk factors for predicting IFI: aminotransferase levels, prothrombin activity, hemoglobin, neutrophil-to-lymphocyte ratio, and serum total bilirubin. A nomogram was constructed incorporating these variables. The nomogram demonstrated good discriminative ability, with an area under the receiver operating characteristic curve (AUC) of 0.78 (95% confidence interval [CI]: 0.72-0.84) in the training cohort and 0.79 (95% CI: 0.70-0.87) in the validation cohort. Decision curve analysis further validated the clinical applicability of the nomogram.</p><p><strong>Conclusion: </strong>ACLF patients with IFI have lower survival time than those without IFI. A nomogram was developed and validated to assist clinicians in the early prediction of IFI in hospitalized patients with ACLF.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"131"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the Priestia megaterium ZS-3 siderophore and studies on its growth-promoting effects.","authors":"Xiao-Xia Zhu, Li-Na Shi, Hui-Min Shi, Jian-Ren Ye","doi":"10.1186/s12866-024-03669-8","DOIUrl":"10.1186/s12866-024-03669-8","url":null,"abstract":"<p><strong>Background: </strong>The ability of plant growth-promoting rhizobacteria (PGPR) to alleviate iron deficiency-induced chlorosis in plants has been widely reported, but the role of siderophores in the re-greening process has rarely been investigated. In this study, the Priestia megaterium ZS-3 (ZS-3) siderophore was first characterized, and a 100-fold concentration of the crude extract of the siderophore was extracted by solid-phase extraction and used to inoculate Arabidopsis thaliana to investigate whether the ZS-3 siderophore could alleviate plant iron deficiency-induced chlorosis in the presence of an insoluble iron source and to determine how it promoted plant growth.</p><p><strong>Results: </strong>The results indicated that -Fe + Fe₂O₃ (Fe₂O₃) treatment induced a decrease in plant growth and iron nutritional status compared with those in the 1/2 MS (one-half-strength Murashige and Skoog medium). Expression levels of representative genes for chlorophyll synthesis, CHLM and CHLG, increased by 85.41% and 77.05% compared to Fe₂O₃ treatment; the IRT1 and FRO₂ in Fe₂O₃ inoculated with the ZS-3 siderophore (T2 treatment) were upregulated by 88.1% and 87.20%, respectively. These results indicate that the ZS-3 siderophore upregulates the expressions of chlorophyll genes to increases photosynthesis and helps plants increase the transcription of iron and the activity of ferric-chelate reductase. Compared with the Fe₂O₃ treatment, the T2 group increased the soluble protein and chlorophyll contents by 2.64- and 3.47-fold, and improved the activities of ferric-chelate reductase and peroxidase (POD) by 3.69- and 2.9-fold, respectively, indicating that the ZS-3 siderophore maintained normal plant growth under Fe₂O₃ stress by increasing the activity of antioxidant enzymes.</p><p><strong>Conclusions: </strong>This study revealed that the ZS-3 siderophore Ferrioxamine E [M + Fe-2 H] enhances plant iron uptake and transport activity at the transcriptional level, confirming the important role of the ZS-3 siderophore in plant iron deficiency status, and the results suggest that the ZS-3 siderophore helps plants acquire iron, alleviates plant chlorosis and promotes plant growth through mechanism I of plant iron acquisition. In this study, we closely linked the structural characterization and quantification of siderophores with Fe deficiency-induced chlorosis to elucidate the promotional mechanism of siderophores in Fe-deficient environments.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"133"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11899797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of soil microbial community structure changes in the drainage field of the Shengli coalfield based on high-throughput sequencing.","authors":"Weixuan Zhao, Ruihong Hou, Mingjian Liu, Haowei Shen, Xiaochen Deng, Mingjiu Wang, Xiangjun Yun","doi":"10.1186/s12866-025-03761-7","DOIUrl":"10.1186/s12866-025-03761-7","url":null,"abstract":"<p><strong>Background: </strong>The study of soil environment in drainage fields is important for environmental management and ecological restoration, and there is currently a knowledge gap in understanding the impact of soil microbial communities in the Shengli coalfield drainage fields and the corresponding ecological effects. To investigate the changes in rhizosphere soil microbial communities of different dominant plants after years of restoration, this study examines the improvement effects of different dominant plants on the soil environment.</p><p><strong>Results: </strong>This study is based on high-throughput sequencing to restore the slope of coal mine spoil after 15 years as the sampling site. The rhizosphere soil of five dominant plants was selected for microbial community analysis, and functional prediction of the microbial community was conducted. The dominant plants selected included Erect Milkvetch (Astragalus adsurgens), Lemongrass (Caragana korshinskii), Alfalfa (Medicago sativa), Phyllanthus pinnatifida (Elymus dahuricus), and Brassica Rapa (Brassica campestris). The results showed that after 15 years of restoration, the soil physicochemical properties in the Phyllanthus pinnatifida group were better than those in the other groups overall, but some of them were inferior to those in the lemon-stripped mallard group. Abundant saprophytic fungal communities were found in different dominant plant groups, mainly belonging to the phyla Ascomycota and Basidiomycota, resulting in significantly higher organic matter content in the dominant plant groups compared to the CK group. The bacterial communities were dominated by the phyla Actinobacteriota, Proteobacteria, Chloroflexi, and Firmicutes. Among these microbial phyla, the Phyllanthus pinnatifida group had higher abundance, which is beneficial for vegetation colonization. Redundancy analysis showed that soil pH was significantly correlated with microbial communities. Organic matter content and pH are the main factors influencing the composition of soil microbial communities, significantly affecting the composition of microorganisms in different groups. After years of restoration, the environment of the Shengli Coalfield's spoil heap has been greatly improved.</p><p><strong>Conclusions: </strong>The planting of various beneficial plants has resulted in significant improvements to the soil microbial community and physicochemical properties, with Phyllanthus pinnatifida having the most positive impact. This lays the foundation for the subsequent restoration of the slope of the spoil heap.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"132"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11899194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual role of KHCO₃ priming: reduces seed-borne pathogens on sorghum seeds and promotes seed germination.","authors":"Shuai Dong, Keqin Peng, Qiyuan Liu, Min Zhang, Yan Luo, Zhen Hua Li","doi":"10.1186/s12866-025-03824-9","DOIUrl":"10.1186/s12866-025-03824-9","url":null,"abstract":"<p><strong>Background: </strong>Seed-borne diseases have seriously affected the sustainability of sorghum cultivation in China as the demand for organic products in the winemaking industry has limited the use of chemical fungicides.</p><p><strong>Results: </strong>This study conducted a comprehensive analysis of fungal diversity in sorghum seeds from three major growing regions in Guizhou Province. Using a combination of traditional tissue separation and high-throughput sequencing, we identified Colletotrichum, Fusarium, Cladosporium, and Alternaria as dominant fungi. Pathogenicity tests revealed that strains GD202206, GD202219, and GD202242 were pathogenic and were identified as C. sublineola through morphological and multi-locus phylogeny analysis (ITS、CAPDH、ACT、CHS-1 and TUB2). 16 fungicides for seed priming experiments with sorghum seeds, the results indicated that priming with KHCO₃ significantly enhanced germination of the sorghum seeds, with both indoor and outdoor emergence rates notably higher. Analysis of the fungal changes before and after KHCO₃ priming revealed a significant reduction in the abundance of the Colletotrichum genus. Additionally, KHCO₃ altered the community structure of fungi within the sorghum seeds, reducing population richness. Inter-generic relationships were rebalanced, with antagonism decreased and synergy increased following KHCO₃ treatment. Non-target metabolomic analysis indicated that KHCO₃ enhances sorghum seed germination via the phenylalanine and flavonoid pathways and exhibits antifungal properties through the cyanoamino acid metabolic pathway.</p><p><strong>Conclusion: </strong>This study identified C. sublineola as the primary pathogenic fungus carried by sorghum seeds. KHCO₃ treatment has a dual effect on sorghum seeds: on one hand, it suppresses pathogen transmission by reducing the abundance of the Colletotrichum genus; on the other hand, it promotes germination and seedling emergence, thereby enhancing both germination and emergence rates.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"134"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different concentrations of chlormequat chloride on bacterial community composition and diversity in peanut soil.","authors":"Qiujun Lin, Xianxin Wu, Chunjing Guo, Lina Li, Tianshu Peng, Xun Zou, Guang Li, Jianzhong Wang","doi":"10.1186/s12866-025-03828-5","DOIUrl":"10.1186/s12866-025-03828-5","url":null,"abstract":"<p><p>The application of pesticides may have significant impacts on soil environment and communities. In order to understand the deep relationship between the application of chlormequat chloride (CC) and the bacterial community in peanut soil, high-resolution characterization was performed using peanut soil samples (12 points; 0-20 cm rhizosphere soil) from untreated and sprayed with different concentrations of CC. Experimental data showed that with the increase of concentration, operational taxonomic units (OTUs) richness showed a decreasing tendency. The OTUs richness at low concentration (D, 50% CC diluted 5000 times, 45 g ai/ha), medium concentration (M, 50% CC diluted 300 times, 75 g ai/ha), and high concentration (G, 50% CC diluted 1000 times, 225 g ai/ha) were 5583, 5430, and 3910, respectively. Low concentrations increased the composition and relative abundance of soil bacterial communities. In contrast, high concentrations significantly reduced bacterial diversity. As the concentration of CC increases, the abundance of Proteobacteria decreases, while the abundance of Firmicutes and Bacteroidetes increases. The number of Acidobacterium and Bacteroidetes increased in groups D and M, while it decreased in group G. D, M and G groups showed a decrease in the abundance of Pseudomonas, polaromonas, and Azovibrio compared to CK, while the abundance of Flavobacterium increased. In addition, the abundance of Rahnella1 decreased in groups D and M, while the abundance increased in group G. The main metabolic pathways included the metabolisms of nucleotides, terpenoids, polyketides, other amino acids, cofactors, vitamins, lipids, glycan biosynthesis, energy, carbohydrates, xenobiotics, amino acids, and other secondary metabolites.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"129"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiome reveals the trophic variation and significant adaption of three sympatric forest-dwelling ungulates on the eastern Qinghai-Xizang Plateau.","authors":"Haonan Zhang, Yichen Wang, Zhengwei Luo, Baofeng Zhang, Xianna Lan, Liancheng Xu, Xuxin Li, Zhixin Huang, Jin Bai, Defu Hu","doi":"10.1186/s12866-025-03812-z","DOIUrl":"10.1186/s12866-025-03812-z","url":null,"abstract":"<p><strong>Background: </strong>The gut microbiome of herbivorous mammals regulates numerous physiological processes, including digestion and energy metabolism. The complex stomach architecture of ruminants, in conjunction with the metabolic capabilities of their microbiota, confers a considerable adaptive advantage to these animals. Nevertheless, a significant gap persists in comparative studies on the variations in the gut microbiome among sympatric ruminants and their potential adaptive implications. Accordingly, in this study, 16S rRNA gene sequencing and metagenomic approaches were used to analyse the composition and functional attributes of the gut microbiome of sympatric Moschus chrysogaster, Capricornis sumatraensis, and Cervus albirostris inhabiting the eastern periphery of the Qinghai-Xizang Plateau.</p><p><strong>Results: </strong>The gut microbiome of C. albirostris exhibited a higher diversity than that of M. chrysogaster and C. sumatraensis, whereas those of M. chrysogaster and C. sumatraensis were similar. Although species-specific variations existed among the three mammalian microbiomes, the microbiomes of C. albirostris and C. sumatraensis were more similar, whereas that of M. chrysogaster was markedly distinct. Metagenomic analysis revealed a pattern of functional convergence in the gut microbiome of the three species, with the gut microbiome of C. albirostris exhibiting a pronounced emphasis on carbohydrate metabolism, significantly surpassing that of M. chrysogaster and C. sumatraensis. Compared to the other two species, the gut microbiome of C. sumatraensis presented significantly elevated levels of amino acids and energy metabolism, whereas that of M. chrysogaster presented an increased capacity for 3-hydroxyacyl- [acyl carrier protein]-dehydratase production.</p><p><strong>Conclusion: </strong>These findings suggest that the gut microbiome of sympatric M. chrysogaster, C. sumatraensis, and C. albirostris tend to converge. Metabolic variations within their gut microbiome may result in differential food resource utilisation, potentially indicating significant nutritional and ecological trait characteristics for stable coexistence.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"128"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulence factors, molecular characteristics, and resistance mechanisms of carbapenem-resistant Pseudomonas aeruginosa isolated from pediatric patients in Shanghai, China.","authors":"Lijun Yin, Zihao Bao, Leiyan He, Lu Lu, Guoping Lu, Xiaowen Zhai, Chuanqing Wang","doi":"10.1186/s12866-025-03856-1","DOIUrl":"10.1186/s12866-025-03856-1","url":null,"abstract":"<p><strong>Background: </strong>The investigation into virulence factors, clinical and molecular characteristics, and resistance mechanisms of carbapenem-resistant Pseudomonas aeruginosa (CRPA) in pediatric populations is currently inadequate.</p><p><strong>Purpose: </strong>This study aimed to investigate the virulence factors, clinical and molecular characteristics, and resistance mechanisms of 135 CRPA isolates in Shanghai, China.</p><p><strong>Methods: </strong>Analysis of virulence-associated genes and multilocus sequence typing (MLST) provided epidemiological and molecular insights into the isolates. Resistance mechanisms were identified via PCR, sequencing, and qRT-PCR.</p><p><strong>Results: </strong>The predominant resistance mechanism to carbapenems was the decreased production of outer membrane porin OprD (75.6%), accompanied by mutational inactivation of the oprD (87.4%). However, elevated production of AmpC (7.4%) and mexB overexpression (5.2%) were uncommon. Thirty-five sequence types (STs) were identified, with clonal complex 244 (CC244;59.3%) representing the majority of infections. Sixteen virulence factor genes were detected, with a significant portion of isolates (40.7%) concurrently possessing Toxin A (toxA), Elastase B (lasB), Exoenzyme S (exoS), staphylolysin (lasA), and Pilin (pilA). Almost all CC244 isolates carried toxA (100%), exoS (100%), pilA (100%), lasB (98.6%), and lasA (82.5%) while all ST2100, ST274, ST1129, ST446, and ST2069 isolates contained exoY. CC244 + isolates exhibited significantly increased antibiotic resistance, and the isolates from diseased or discharged patients showed comparatively higher resistance than others, except against gentamicin. Most patients (71.9%) received combination therapy, with 65.2% achieving clinical cure or improvement.</p><p><strong>Conclusion: </strong>This study predominantly identified OprD-mediated carbapenem resistance in pediatric patients. The CRPA isolates were characterized by a variety of STs and a widespread distribution of virulence-associated genes. CC244 demonstrated significantly higher resistance, with potential outbreaks occurring in 2018 and 2019. These findings could aid in managing nosocomial CRPA infections and enhancing clinical practices.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"130"},"PeriodicalIF":4.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of katG and inhA mutations associated with isoniazid resistance in Mycobacterium tuberculosis clinical isolates in Cameroon.","authors":"Vanessa Ninkeh Nono, Edouard Akono Nantia, Awelani Mutshembele, Sorelle Nguimfack Teagho, Yannick Willy Kamdem Simo, Brenda Shile Takong, Yvonne Josiane Djieugoue, Yannick Patrick Assolo, Suzanne Magloire Ongboulal, Stanley Nkemnji Awungafac, Sara Eyangoh, Eric Mensah, Ndivhuho Agnes Makhado, Valerie Flore Donkeng Donfack","doi":"10.1186/s12866-025-03816-9","DOIUrl":"10.1186/s12866-025-03816-9","url":null,"abstract":"<p><strong>Background: </strong>The acquisition of isoniazid (INH) resistance is alarming, considering its importance as a key drug that forms the core of multidrug treatment regimens for tuberculosis (TB). Genetic mutations in the katG and inhA promoter regions play crucial roles in INH resistance, but their prevalence varies geographically. This study aimed to identify the most common mutations in the katG and inhA genes in INH-resistant (INH-R) Mycobacterium tuberculosis (MTB) clinical isolates in Cameroon. The research also explored the relationships between these mutations and patients' demographics (age, sex, and sample type).</p><p><strong>Methods: </strong>We conducted a retrospective cross-sectional laboratory-based study on 500 INH-R isolates (with or without resistance to other first-line drugs) at the National Tuberculosis Reference Laboratory (NTRL) in Cameroon between January 2014 and December 2020. GenoType MTBDRplus assay was performed on the retrieved isolates and the frequency of katG and inhA mutations were calculated. Chi-square tests were utilized to assess the associations between these mutations and patients' age, sex and sample type.</p><p><strong>Results: </strong>A total of 410 (85.8%) culture-positive MTB isolates were analyzed, with a male-to-female ratio of 228 (55.6%) to 182 (44.4%) and an average age of 36.3 ± 13.4 years. Mutations in the katG and inhA genes were detected in 354 (86.3%) of cases, while 56 (13.7%) showed no mutations. Among the INH-R isolates, mutations in katG, inhA, and dual katG and inhA genes were present in 247 (60.2%), 76 (18.5%), and 31 (7.6%) isolates, respectively. Our analysis revealed significant associations between mutation prevalence and patient characteristics.</p><p><strong>Conclusion: </strong>This study reaffirmed the importance of the katG S315T substitution as a key indicator of INH resistance, with the inhA C-15T mutation providing additional support. However, a notable proportion of isoniazid-resistant isolates did not exhibit these mutations, underscoring the need to comprehend resistance mechanisms. Given that these mechanisms are strongly associated with varying levels of INH resistance, it is crucial that TB management strategies incorporate genetic profiling alongside patient demographics to optimize treatment outcomes and enhance control measures.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"127"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of Mycobacterium smegmatis small noncoding RNA B11 in triacylglycerol accumulation and altered cell wall permeability.","authors":"Zhuhua Wu, Weilong Liu, Qiuchan Tan, Yuhui Chen, Xiaoyu Lai, Jianming Hong, Hongdi Liang, Huizhong Wu, Jing Liang, Xunxun Chen","doi":"10.1186/s12866-025-03826-7","DOIUrl":"10.1186/s12866-025-03826-7","url":null,"abstract":"<p><strong>Background: </strong>Pathways involving triacylglycerol (TAG) accumulation are thought to play a crucial regulatory role in bacterial growth and metabolism. Despite this understanding, little is known about the biological functions and regulatory mechanisms of small RNAs in Mycobacterium. Mycobacterium smegmatis (M. smegmatis), a type of Mycobacterium, serves as a model organism to investigate the molecular, physiological, and drug resistance features of M. tuberculosis.</p><p><strong>Results: </strong>In this study, we demonstrated that overexpression of B11 significantly affects bacterial growth and colony morphology, increases antibiotic sensitivity and sodium dodecyl sulfate (SDS) surface stress, decreases intracellular survival, and suppresses cytokine secretion in macrophages. Transcriptomic and lipidomic analyses revealed a metabolic downshift in the B11 overexpression strain, characterized by reduced levels of TAG. Furthermore, transmission electron microscopy showed that the B11 overexpression strain exhibited decreased cell wall thickness, leading to reduced biofilm formation and altered cell wall permeability. Additionally, we observed that B11 regulated certain target genes but did not directly bind to those proteins tested.</p><p><strong>Conclusions: </strong>Taken together, these findings suggest that B11 plays important roles in Mycobacterium survival under antibiotic and SDS stresses, TAG accumulation, and contributes to antibiotic sensitivity through altered cell wall permeability.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"124"},"PeriodicalIF":4.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}