BMC Microbiology最新文献

{"title":"Gamma-irradiated copper-based metal organic framework nanocomposites for photocatalytic degradation of water pollutants and disinfection of some pathogenic bacteria and fungi.","authors":"Gharieb S El-Sayyad, Ahmed M El-Khawaga, Huda R M Rashdan","doi":"10.1186/s12866-024-03587-9","DOIUrl":"10.1186/s12866-024-03587-9","url":null,"abstract":"<p><strong>Background: </strong>Although there are many uses for metal-organic framework (MOF) based nanocomposites, research shows that these materials have received a lot of interest in the field of water treatment, namely in the photodegradation of water contaminants, and disinfection of some pathogenic bacteria and fungi. This is brought on by excessive water pollution, a lack of available water, low-quality drinking water, and the emergence of persistent micro-pollutants in water bodies. Photocatalytic methods may be used to remove most water contaminants, and pathogenic microbes, and MOF is an excellent modifying and supporting material for photocatalytic degradation.</p><p><strong>Methods: </strong>This work involved the fabrication of a unique Cu-MOF based nanocomposite that was exposed to gamma radiation. The nanocomposite was subsequently employed for photocatalytic degradation and as an antimicrobial agent against certain harmful bacteria and fungi. The produced Cu-MOf nanocomposite was identified by XRD, SEM, and EDX. Growth curve analysis, UV lighting impact, and antibiofilm potential have been carried out to check antimicrobial potential. Additionally, the membrane leakage test was used to determine the mechanism of the antimicrobial action. In an experimental investigation of photocatalytic activity, a 50 mL aqueous solution including 10.0 ppm of Rhodamine B (RB) was used to solubilize 10 mg of Cu-MOF. It has been investigated how pH and starting concentration affect RB elimination by Cu-MOF. Ultimately, RB elimination mechanism and kinetic investigations have been carried out.</p><p><strong>Results: </strong>SEM images from the characterization techniques demonstrated the fact that the Cu-MOF was synthesized effectively and exhibited the Cu-MOF layers' flake-like form. Uneven clusters of rods make up each stratum. The primary peaks in the Cu-MOF's diffraction pattern were found at 2θ values of 8.75<sup>◦</sup>, 14.83<sup>◦</sup>, 17.75<sup>◦</sup>, 21.04<sup>◦</sup>, 22.17<sup>◦</sup>, 23.31<sup>◦</sup>, 25.41<sup>◦</sup>, and 26.38<sup>◦</sup>, according to the XRD data. After 135 min of UV irradiation, only 8% of RB had undergone photolytic destruction. On the other hand, the elimination resulting from adsorption during a 30-min period without light was around 16%. Conversely, after 135 min, Cu-MOF's photocatalytic breakdown of RB with UV light reached 81.3%. At pH 9.0, the greatest removal of RB at equilibrium was found, and when the amount of photocatalyst rose from 5 to 20 mg, the removal efficiency improved as well. The most sensitive organism to the synthesized Cu-MOF, according to antimicrobial data, was Candida albicans, with a documented MIC value of 62.5 µg mL<sup>-1</sup> and antibacterial ZOI as 32.5 mm after 1000 ppm treatment. Cu-MOF also showed the same MIC (62.5 µg mL<sup>-1</sup>) values against Staphylococcus aureus and Escherichia coli, and 35.0 and 32.0 mm ZOI after 1000 ppm treatment, respectively. Ultimately","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"453"},"PeriodicalIF":4.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11539452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus rhamnosus modulates murine neonatal gut microbiota and inflammation caused by pathogenic Escherichia coli.","authors":"Hao Xuan, Shahid Umar, Cuncong Zhong, Wei Yu, Ishfaq Ahmed, Joshua L Wheatley, Venkatesh Sampath, Susana Chavez-Bueno","doi":"10.1186/s12866-024-03598-6","DOIUrl":"10.1186/s12866-024-03598-6","url":null,"abstract":"<p><strong>Background: </strong>Pathogenic Escherichia coli strains produce neonatal septicemia after colonizing the neonatal gut. While the probiotic Lactobacillus rhamnosus GG (LGG) effectively reduces neonatal sepsis, LGG's effects on the neonatal intestinal microbiota alterations and inflammation triggered by E. coli are incompletely understood. We hypothesized that LGG significantly modulates the specific neonatal gut microbial populations changes and the inflammatory response elicited by the enteral introduction of septicemia-producing E. coli. To test this hypothesis, newborn rats were pretreated orally with LGG or placebo prior to infection with the neonatal E. coli septicemia clinical isolate SCB34. Amplicon 16S rRNA gene sequencing was performed on intestinal samples. Intestinal injury and expression of inflammatory mediators and apoptosis were determined.</p><p><strong>Results: </strong>Alpha diversity of gut microbiota was greater in SCB34-infected pups in comparison to sham-infected pups, these changes were not modified by LGG pretreatment. Beta diversity analyses also showed differences between SCB34-infected vs. uninfected pups. LGG pretreatment before SCB34 infection did not result in significant beta diversity changes compared to placebo. Moreover, individual genera and species abundance analyses by linear discriminant analysis effect size (LEfSe) showed significant changes in Gram-negative, Gram-positive, and anaerobic populations resulting from LGG pretreatment and SCB34 infection. LGG significantly suppressed the expression of inflammatory cytokines but did not attenuate SCB34-induced apoptosis or histologic injury.</p><p><strong>Conclusions: </strong>LGG modulates clinically significant microbiota features and inflammation triggered by pathogenic E. coli intestinal infection shortly after birth. This new knowledge can potentially be harnessed to design novel interventions against gut-derived neonatal sepsis.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"452"},"PeriodicalIF":4.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11539828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere bacterial community is mainly determined by soil environmental factors, but the active bacterial diversity is mainly shaped by plant selection.","authors":"Yalong Xu, Jingjing Li, Chan Qiao, Jinchu Yang, Juan Li, Xueao Zheng, Chen Wang, Peijian Cao, Yan Li, Qiansi Chen","doi":"10.1186/s12866-024-03611-y","DOIUrl":"10.1186/s12866-024-03611-y","url":null,"abstract":"<p><strong>Background: </strong>The assembly of the rhizosphere community, even the diazotroph community, is mainly shaped by soil environmental factors (including soil climate and physiochemical characteristics) and plant selection. To better understand the driving forces on the active overall and nitrogen-fixing bacterial community compositions, we characterized the communities of tobacco rhizosphere soil collected from three sampling sites with a large geographic scale (> 600 km).</p><p><strong>Results: </strong>The results indicate that the diversity and community composition of the overall bacterial and diazotroph communities are obviously differed according to the sampling sites. Still, no significant difference is found between the communities in rootzone and rhizosphere samples. Climate variables including mean annual precipitation (MAP) and mean annual temperature (MAT), soil physiochemical characteristics including available nitrogen (AN), available potassium (AK) and pH are main factors that affect the bacterial and diazotroph community structures in the three sampling sites. Furthermore, MAP and MAT, AN and available phosphorus (AP), total nitrogen (TN) and organic carbon (OC), AK and electrical conductivity (EC) showed similar effects, but pH showed independent effect on the composition of the overall bacteria and diazotroph communities. However, the alpha diversity indices of active overall and nitrogen-fixing bacteria in the rhizosphere are obviously higher than in the rootzone samples, and no significant differences are observed among different sampling sites. Proteobacteria is the predominant active phylum of all samples for overall and nitrogen-fixing bacteria. Escherichia-Shigella, Achromobacter, Streptomyces and Sphingomonas are the dominant active bacterial genera, and Bradyrhizobium, Skermanella and Extensimonas are dominant active nitrogen-fixing bacteria genera in rhizosphere. Furthermore, the high active abundance of Escherichia-Shigella but low abundance of Ralstonia in all three sampling sites indicate high root-knot nematode infection and low wilt disease endemic risk.</p><p><strong>Conclusion: </strong>These results indicate that soil environmental factors contribute more to the tobacco rhizosphere bacterial community assemblage, but the rhizosphere contributes more to the diversity of active overall bacteria and nitrogen-fixing bacteria in the community. Our study provides novel knowledge for the assemble of rhizosphere bacterial and active bacteria communities across a large geographical scale.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"450"},"PeriodicalIF":4.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of three methods for generating the coccoid form of Helicobacter pylori and proteomic analysis.","authors":"Kyoungwon Jung, Haram Bae, Jiyeun Kate Kim, Bohyun Jeong, Moo In Park, Jee Young Lee","doi":"10.1186/s12866-024-03599-5","DOIUrl":"10.1186/s12866-024-03599-5","url":null,"abstract":"<p><strong>Background: </strong>Helicobacter pylori changes from spiral to coccoid depending on the host state, environmental factors, and surrounding microbial communities. The coccoid form of H. pylori still maintains its complete cellular structure, retains virulence genes, and thus plays a role in pathogenicity. To understand the coccoid form, it is crucial to establish the in vitro generation of the coccoid H. pylori. Although some conditions have been studied for the generation of the coccoid form, few studies have compared these conditions for coccoid generation. Here, we generated coccoid forms via three methods and compared the differences in morphology, viability, culturability, and protein expression.</p><p><strong>Results: </strong>The coccoid H. pylori was generated in vitro via three methods: a starvation method, a method using amoxicillin, and a method using the culture supernatant of Streptococcus mitis. The morphology and viability of the cells were examined by fluorescence microscopy after staining with SYTO9 and propidium iodide. The culturability of H. pylori was examined by counting colony-forming units on chocolate agar plates. In the starvation group, no colonies formed after 7 days, but viable coccoids were continuously observed. In the amoxicillin-treated group, the culturability decreased rapidly after 12 h, and showed a viable but non culturable (VBNC) state after the third day. Most cells treated with S. mitis supernatant changed to coccoid forms after 7 days, but colonies were continuously formed, probably due to living spiral forms. We performed proteomics to analyse the differences in protein profiles between the spiral and coccoid forms and protein profiles among the coccoid forms generated by the three methods.</p><p><strong>Conclusion: </strong>Amoxicillin treatment changed H. pylori to VBNC cells faster than starvation. Treatment with the S. mitis supernatant prolonged the culturability of H. pylori, suggesting that the S. mitis supernatant may contain substances that support spiral form maintenance. Proteomic analysis revealed that the expression of proteins differed between the spiral form and coccoid form of H. pylori, and this variation was observed among the coccoid forms produced via three different methods. The proteins in the coccoid forms produced by the three methods differed from each other, but common proteins were also observed among them.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"448"},"PeriodicalIF":4.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of two Friunavirus phages and their inhibitory effects on biofilms of extremely drug resistant Acinetobacter baumannii in Dakar, Senegal.","authors":"Issa Ndiaye, Laurent Debarbieux, Ousmane Sow, Bissoume Sambe Ba, Moussa Moise Diagne, Abdoulaye Cissé, Cheikh Fall, Yakhya Dieye, Ndongo Dia, Guillaume Constantin de Magny, Abdoulaye Seck","doi":"10.1186/s12866-024-03608-7","DOIUrl":"10.1186/s12866-024-03608-7","url":null,"abstract":"<p><strong>Background: </strong>Acinetobacter baumannii is a gram-negative, opportunistic pathogen, that is responsible for a wide variety of infections and is a significant cause of hospital-acquired infections. A. baumannii is listed by the World Health Organization (WHO) as a critical priority pathogen because of its high level of antibiotic resistance and the urgent need for alternative treatment solutions. To address this challenge, bacteriophages have been used to combat bacterial infections for more than a century, and phage research has regained interest in recent years due to antimicrobial resistance (AMR). However, although the vast majority of deaths from the AMR crisis will occur in developing countries in Africa and Asia, few phages' studies have been conducted in these regions. In this study, we present a comprehensive characterization of the bacteriophages vAbBal23 and vAbAbd25, actives against extremely drug-resistant (XDR) A. baumannii.</p><p><strong>Methods: </strong>Phages were isolated from environmental wastewaters in Dakar, Senegal. The host-range, thermal and pH stabilities, infection kinetics, one step growth assay, antibiofilm activity assay, sequencing, and genomic analysis, were performed to characterize the isolated phages.</p><p><strong>Results: </strong>Comparative genomic and phylogenetic analyses revealed that vAbBal23 and vAbAbd25 belong to the Caudoviricetes class, Autographiviridae family and Friunavirus genus. Both phages demonstrated activity against strains with capsular type KL230. They were stable over a wide pH range (pH 3 to 9) and at temperatures ranging from 25 °C to 40 °C. Additionally, the phages exhibited notable activity against both planktonic and biofilm cells of targeted extremely drug resistant A. baumannii. The results presented here indicate the lytic nature of vAbBal23 and vAbAbd25. This is further supported by the absence of genes encoding toxins, resistance genes and bacterial virulence factors, highlighting their potential for future phage applications.</p><p><strong>Conclusion: </strong>Phages vAbBal23 and vAbAbd25 are promising biological agents that can infect A. baumannii, making them suitable candidates for use in phage therapies.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"449"},"PeriodicalIF":4.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variable sensitivity of clinical Candida auris strains to Biocides: implications for infection control in Healthcare Settings.","authors":"Sidre Erganis, Ali Ozturk, Sema Turan Uzuntas, Fusun Kirca, Alper Dogan, Bedia Dinc, Ayse Kalkanci","doi":"10.1186/s12866-024-03605-w","DOIUrl":"10.1186/s12866-024-03605-w","url":null,"abstract":"<p><strong>Purpose: </strong>Candida auris, a multidrug-resistant yeast, poses significant challenges in healthcare settings due to its ability to form biofilms and resistance to common disinfectants. Understanding its susceptibility to biocides used in hospital disinfection practices is crucial for infection control. We investigated the biocide sensitivity of eight clinical C. auris strains from different patients and one reference strain (CDC B11903) using the biocide activity tests.</p><p><strong>Methods: </strong>Species identification was confirmed through MALDI-TOF MS, while clade differentiation and phylogenetic classification were determined via whole-genome sequencing. Biofilm formation was assessed using the MTT assay. Antifungal susceptibilities were tested according to CLSI standards. The effectiveness of biocides, including chlorine, chlorhexidine, and benzalkonium chloride, was evaluated through broth microdilution following CLSI standards and quantitative suspension and carrier tests, following EN standards.</p><p><strong>Results: </strong>All clinical strains were identified as clade 1, and the reference strain as clade 4, with all exhibiting biofilm formation. Clade 1 strains showed resistance to fluconazole, with MIC values ranging from 8 to 32 µg/ml, while being susceptible to other antifungals. Broth microdilution MIC assays for biocides demonstrated that all strains exhibited resistance to benzalkonium chloride. Chlorine and chlorhexidine showed variable efficacy, dependent on concentration and environmental cleanliness. Alcohol-based hand sanitizers demonstrated effectiveness against C. auris from the first minute of application.</p><p><strong>Conclusion: </strong>The study highlights the variable susceptibility of C.auris to different biocides, underscoring the challenge in eradicating this pathogen from healthcare environments. Our findings advocate for the careful selection of disinfectants in hospital settings, emphasizing the need for high-concentration chlorine and chlorhexidine solutions to combat C. auris, even in especially clean environments.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"447"},"PeriodicalIF":4.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flue-cured tobacco intercropping with insectary floral plants improves rhizosphere soil microbial communities and chemical properties of flue-cured tobacco.","authors":"Jin Zhong, Wenze Pan, Shenglan Jiang, Yanxia Hu, Guangyuan Yang, Ke Zhang, Zhenyuan Xia, Bin Chen","doi":"10.1186/s12866-024-03597-7","DOIUrl":"10.1186/s12866-024-03597-7","url":null,"abstract":"<p><strong>Background: </strong>Continuous cropping of the same crop leads to land degradation. This is also called the continuous-cropping obstacle. Currently, intercropping tobacco with other crops can serve as an effective strategy to alleviate continuous cropping obstacles.</p><p><strong>Results: </strong>In this study, tobacco K326 and insectary floral plants were used as materials, and seven treatments of tobacco monoculture (CK), tobacco intercropped with Tagetes erecta, Vicia villosa, Fagopyrum esculentum, Lobularia maritima, Trifolium repens, and Argyranthemum frutescens respectively, were set up to study their effects on rhizosphere soil chemical properties and composition and structure of rhizosphere soil microbial community of tobacco. The 16 S rRNA gene and ITS amplicons were sequenced using Illumina high-throughput sequencing. tobacco/insectary floral plants intercropping can influence rhizosphere soil chemical properties, which also change rhizosphere microbial communities. The CK and treatment groups tobacco rhizosphere soil microorganisms had significantly different genera, such as tobacco intercropping with T. repens and A. frutescens significantly increased the number of Fusarium and intercropping T. erecta, V. villosa, L. maritima, T. repens, and A. frutescens significantly increased the number of Sphingomonas and unknown Gemmatimonadaceae. Additionally, intercropping T. erecta, V. villosa and L. maritima changed the rhizosphere fungal and bacteria community and composition of tobacco and the positive correlation between tobacco rhizosphere the genera of fungi and bacterial were greater than CK. The pathway of the carbohydrate metabolism, amino acid metabolism, and energy metabolism in rhizosphere bacteria were significantly decreased after continuous cropping. Fungal symbiotic trophic and saprophytic trophic were significantly increased after intercropping V. villosa, L. maritima and plant pathogen and animal pathogen were increased after intercropping T. repens and A. frutescens. Additionally, bacterial and fungal communities significantly correlated with soil chemical properties, respectively.</p><p><strong>Conclusion: </strong>This study reveals that intercropping tobacco with insectary floral plants, particularly T. erecta, V. villosa, L. maritima and A. frutescens significantly affects soil chemical properties and alters rhizosphere microbial communities, increasing the abundance of certain microbial genera. Additionally, intercropping enhances pathways related to carbohydrate, amino acid, and energy metabolism in rhizosphere bacteria. These findings suggest that intercropping could provide a promising strategy to overcome challenges associated with continuous tobacco cropping by regulating the rhizosphere environment.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"446"},"PeriodicalIF":4.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaginal microbiota in term pregnant women with differences in cervical ripeness revealed by 2bRAD-M.","authors":"Saihu Lu, Qi Wu, Wenzhu He, Xue Du, Qianqian Cui, Yuanyuan Yang, Zongzhi Yin","doi":"10.1186/s12866-024-03612-x","DOIUrl":"10.1186/s12866-024-03612-x","url":null,"abstract":"<p><strong>Background: </strong>Cervical ripening is a multifactorial outcome, and the association between cervical ripening and vaginal microbiota remains unexplored in term primiparous women. A new sequencing technology, microbiome 2bRAD sequencing (2bRAD-M) that provides a higher level of species discrimination compared to amplicon sequencing. We applied 2bRAD-M to analyze the vaginal microbiota in a population with variations in cervical ripeness and to explore potential microbiota factors influencing cervical ripening.</p><p><strong>Methods: </strong>A total of 30 full-term primigravid women participated in this study, with 15 belonging to the low scoring group of cervical ripeness and 15 to the high scoring group. Clinical information was collected from the participants, and the vaginal microbiota and community structure of both groups were analyzed using 2bRAD-M sequencing. Microbiota diversity and differential analyses were conducted to explore potential factors influencing cervical ripening.</p><p><strong>Results: </strong>A total of 605 species were detected. There was no difference in vaginal microbiota diversity between the two groups, and the vaginal microbial composition was structurally similar. In the two groups, Lactobacillus crispatus and Lactobacillus iners were identified as the two pivotal species through random forest analysis. Concurrent, extensive and close connections between species within the two groups were observed in the correlation analysis, influencing the aforementioned two species. Pairwise comparisons showed that Sphingomonas (P = 0.0017) and three others were abundant in high scoring group, while Alloprevotella (P = 0.0014), Tannerella (P = 0.0033), Bacteroides (P = 0.0132), Malassezia (P = 0.0296), Catonella (P = 0.0353) and Pseudomonas (P = 0.0353) and so on showed higher abundance in low scoring group. Linear discriminant analysis effect size identified 29 discriminative feature taxa.</p><p><strong>Conclusion: </strong>For the first time, vaginal microbiota was sequenced using 2bRAD-M. With a relatively simple structure, a more stable vaginal microbiota is associated with higher cervical ripeness, and certain microorganisms, such as Sphingomonas, may play a beneficial role in cervical ripening.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"444"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyrtios sp.-associated Cladosporium sp. UR3 as a potential source of antiproliferative metabolites.","authors":"Omnia Hesham Abdelhafez, Abeer H Elmaidomy, Mohamed Hisham, Stefanie P Glaeser, Peter Kämpfer, Jun Wu, Usama Ramadan Abdelmohsen","doi":"10.1186/s12866-024-03560-6","DOIUrl":"10.1186/s12866-024-03560-6","url":null,"abstract":"<p><strong>Background: </strong>Sponge-associated microorganisms are promising resources for the production of bioactive compounds with cytotoxic potential. The main goal of our study is to isolate the fungal endophytes from the Red Sea sponge Hyrtios sp. followed by investigating their cytotoxicity against number of cell lines.</p><p><strong>Results: </strong>The fungal strain UR3 was isolated from the Red Sea sponge using Sabouraud dextrose agar media. It was identified based on partial 18 S rRNA gene and ITS sequence analyses as Cladosporium sp. UR3. The in vitro cytotoxic potential of the ethyl acetate extract of the fungal isolate was evaluated using MTT assay against three cancer cell lines: CACO2, MCF7, and HEPG2. Metabolomics profiling of the obtained ethyl acetate extract using LC-HR-ESI-MS, along with molecular docking and pharmacological network studies for the dereplicated compounds were performed to explore its chemical profile and the possible cytotoxic mechanism of the sponge-associated fungi.</p><p><strong>Conclusion: </strong>These results highlighted the role of sponge-associated fungi as a fruitful resource for the discovery of cytotoxic metabolites.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"445"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic potential of Leu₁₀-teixobactin and cefepime against multidrug-resistant Staphylococcus aureus.","authors":"Augustine Jing Jie Koh, Maytham Hussein, Varsha Thombare, Simon Crawford, Jian Li, Tony Velkov","doi":"10.1186/s12866-024-03577-x","DOIUrl":"10.1186/s12866-024-03577-x","url":null,"abstract":"<p><p>Staphylococcus aureus (S. aureus) is a significant Gram-positive opportunistic pathogen behind many debilitating infections. β-lactam antibiotics are conventionally prescribed for treating S. aureus infections. However, the adaptability of S. aureus in evolving resistance to multiple β-lactams contributed to the persistence and spread of infections, exemplified in the emergence of methicillin-resistant S. aureus (MRSA). In the present study, we investigated the efficacies of the synthetic teixobactin analogue, Leu₁₀-teixobactin, combined with the penicillinase-resistant cephalosporin cefepime against MRSA strains. The Leu₁₀-teixobactin and cefepime combination exerted synergism against most strains tested in broth microdilution assay. Time-kill profiles showed that both Leu₁₀-teixobactin and cefepime predominantly exhibited synergistic activity, with > 2.0-log₁₀CFU decrease compared to monotherapy at 24 h. Moreover, biofilm assays revealed a significant inhibition of biofilm production in ATCC™43300 cells treated with sub-MICs of Leu₁₀-teixobactin and cefepime. Subsequent electron microscopy studies showed more extensive damage with the combination therapy compared to monotherapies, including aberrant bacterial morphology, vesicle formation and substantial lysis, indicating combined damage to the cell wall. Quantitative real-time PCR revealed marked perturbation of genes mecA, sarA, atlA, and icaA, substantiating the apparent mode of combined antibacterial action of both antibiotics against peptidoglycan synthesis and initial biofilm production. Hence, the study highlights the prospective utility of the Leu₁₀-teixobactin-cefepime combination in treating MRSA infections via β-lactam potentiation.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"442"},"PeriodicalIF":4.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}