BMC Microbiology最新文献

{"title":"Correction: Pan-genome and antibiotic resistance insights into Xanthomonas citri pv. punicae pathotypes.","authors":"V Rathna, Aditya Kukreti, M K Prasannakumar, Manjunatha C, Karan R, Swathi S Patil, Gopal Venkateshbabu, Pramesh Devanna, Harish J, Sahana N Banakar, Aditya Narayan, Keerthi Vaidya, Shibormi Rymbai, H B Mahesh, Raju Y Soolanayakanahally, Sateesh Kagale","doi":"10.1186/s12866-026-05400-1","DOIUrl":"10.1186/s12866-026-05400-1","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"26 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Depth-dependent differences between direct total DNA and an intracellular-DNA-enriched fraction in bulk-soil bacterial and fungal communities of a Lonicera japonica field.","authors":"Chenyi Mao, Xiuling Wang","doi":"10.1186/s12866-026-05436-3","DOIUrl":"10.1186/s12866-026-05436-3","url":null,"abstract":"<p><strong>Background: </strong>Direct total-DNA (tDNA) extraction from soil recovers both cell-associated and extracellular (relic) DNA, which can distort amplicon-based views of microbial communities; how strongly this biases bacteria versus fungi, and whether it varies with soil depth, remains poorly resolved for cultivated soils. Using a paired split-sample design, we compared bacterial 16S rRNA gene and fungal ITS profiles from directly extracted tDNA and an operationally defined intracellular-DNA-enriched (iDNA) fraction in bulk soil sampled at three depths from a continuously cultivated Lonicera japonica field.</p><p><strong>Results: </strong>The two DNA pools gave related but clearly non-interchangeable pictures of the community, and the two kingdoms responded asymmetrically: bacteria were consistently the more sensitive, with higher richness recovered from tDNA and a marked separation between DNA pools throughout the profile, whereas the fungal contrast was smaller overall and, for alpha diversity and within-pair divergence, concentrated in the surface soil. DNA-pool choice acted mainly by selectively redistributing specific lineages rather than replacing the dominant community, while the measured soil physicochemical variables explained only a small share of community variation within either pool.</p><p><strong>Conclusions: </strong>Direct total DNA and an iDNA-enriched fraction offer kingdom- and depth-dependent windows on the same soil, so the choice of DNA pool is a consequential design decision-especially for bacteria and for surface horizons-that should be matched to the ecological question and independently validated when a cell-associated fraction is the goal. More broadly, a single total-DNA snapshot cannot be assumed to represent the cell-associated community equally across taxa or depths; treating the DNA-extraction strategy as an ecological variable rather than a neutral technical step would strengthen the comparability and interpretation of bulk-soil bacterial and fungal community surveys.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13670670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ruminal bacterial and protozoal features associated with milk yield in Chinese Holstein cows.","authors":"Jing Yang, A La Teng Zhu La, Shouqian Zhu, Jie Qi, Mei Sun, Xiaoyu Niu, Dabiao Li, Yuanyuan Xing","doi":"10.1186/s12866-026-05411-y","DOIUrl":"10.1186/s12866-026-05411-y","url":null,"abstract":"<p><strong>Background: </strong>Milk yield is a key economic trait in dairy cows and is closely linked to rumen microbial function. However, the associations between rumen microbial communities and milk yield remain incompletely understood. This study compared the rumen microbial composition and function of Chinese Holstein cows with high and low milk yields to clarify the relationships between differential rumen microbes and lactation performance.</p><p><strong>Results: </strong>Results showed that HY cows had higher milk yield, milk fat yield, milk protein yield, lactose yield, and total milk solids (TMS) yield (P < 0.05), but lower milk fat concentration (P < 0.05). Regarding rumen fermentation, the yields of propionic acid and microbial crude protein (MCP) were increased in the HY group (P < 0.05), whereas the concentration of acetic acid and the acetic acid/propionic acid (A/P) ratio were significantly reduced (P < 0.05). Additionally, neutral detergent fiber (NDF) digestibility was higher in the HY group compared to the LY group (P < 0.05). 16 S rRNA gene sequencing revealed that the relative abundance of the bacterial genera such as Succiniclasticum, Prevotella, and Ruminococcus was increased in the HY group, while 18 S rRNA gene sequencing showed that the relative abundance of the protozoan genus such as Entodinium was higher in the HY group (P < 0.05). Furthermore, the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) analysis revealed that the pyruvate fermentation to propanoate I pathway and the synthesis-related enzymes within this pathway were upregulated in the HY group. Notably, we found that Prevotella and Succiniclasticum were positively correlated with milk yield and propionic acid concentration, whereas Entodinium was positively correlated with Prevotella, Succiniclasticum, and Ruminococcus, and the partial least square-structural equation model (PLS-SEM) revealed that rumen microbes influence milk yield via enhanced rumen fermentation and nutrient digestibility.</p><p><strong>Conclusions: </strong>Collectively, HY cows exhibited higher NDF digestibility, propionic acid concentration, and MCP production than LY cows, and also showed higher abundances of Prevotella, Succiniclasticum, Ruminococcus, and Entodinium, as well as enrichment of the pyruvate fermentation to propanoate I pathway. These microbial characteristics were associated with differences in rumen function and milk yield between HY and LY dairy cows.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High soil fertility supports greater soil quality, microbial network complexity, and keystone taxa in arable black soil.","authors":"Feng Shi, Kang-Kang Wang, Chong Luo, Pin-Jiao Jin, Huan-Jun Liu, Shuang Wang","doi":"10.1186/s12866-026-05412-x","DOIUrl":"10.1186/s12866-026-05412-x","url":null,"abstract":"<p><p>The depletion of soil organic matter (SOM) represents one of the most conspicuous hallmarks of Mollisol degradation in Northeast China. This study focuses on typical black soil with high, medium, and low fertility levels to investigate the responses of soil properties and microbial communities to varying soil fertility. We observed a significant decline in the Soil Quality Index (SQI) and key enzyme activities from high to low fertility levels, which mirrored the synchronous depletion of soil carbon fractions (SOM, POC, and MBC). Our results indicated that while microbial alpha diversity peaked in soils with medium fertility, overarching network complexity was highest in environments with high fertility. Compositionally, this robust community structure was primarily associated with significant increases in the abundance of Actinobacterota and Mortierellomycota. Furthermore, network analysis identified specific keystone taxa, including Gemmatimonadaceae, Roseiflexaceae, Penicillium, and Trichoderma, which are closely associated with efficient nutrient cycling and enhanced disease resistance. This study elucidates the microbial taxa associated with high-fertility soils. And by elucidating these underlying microbial associations, this study provides a solid theoretical foundation for restoring degraded black soils through targeted microbiome regulation. Ultimately, our findings highlight promising avenues for developing novel soil conservation technologies centered on core functional microorganisms.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13670726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of intestinal protozoa in an oil wastewater treatment facility; risk for contamination of downstream farmlands.","authors":"Hanieh Mohammad-Rahimi, Saeid Andalib, Maryam Niyyati, Hamed Mirjalali","doi":"10.1186/s12866-026-05419-4","DOIUrl":"10.1186/s12866-026-05419-4","url":null,"abstract":"<p><strong>Background: </strong>Oil refinery (OR) facilities consume vast volumes of water during their processes, generating a large amount of wastewater (WW). The oil refinery wastewater (ORWW) contains toxic materials, and few microorganisms are believed to remain active in this environment.</p><p><strong>Methods: </strong>Water samples were collected from an ORWW facility for nine months. After recording physical-chemical features, filtration and sedimentation were employed. DNA extraction was performed for all samples and the presence of targeted parasites was evaluated using real-time PCR. Genotype/subtype of positive samples was characterized using sequencing.</p><p><strong>Results: </strong>Real-time PCR showed that from 76 ORWW samples, targeted protozoa were identified in 26 (34.2%) samples. Accordingly, Blastocystis sp., Enterocytozoon bieneusi, and Giardia duodenalis were detected among 9.21% (7/76), 25.0% (19/76), and 2.63% (2/76) of samples, respectively. Enterocytozoon bieneusi was the most common detected species in samples followed by Blastocystis sp. and G. duodenalis. There was a statistically association between the presence of Blastocystis sp. and the type of wastewater treatment plants (WWTPs). Enterocytozoon bieneusi genotypes BEB6 (3/5; 60%), C (1/5; 20%), and an unknown genotype were detected among samples. Also, Blastocystis sp., ST1 (2/4; 50%), ST3 (1/4; 25%), uncultured uncharacterized stramenopile (1/4; 25%) and ST2 (1; 100%), were characterized in samples. Giardia duodenalis was the assemblage AII (1/2; 50%). Phylogenetic analysis showed that E. bieneusi genotypes were grouped within the groups 1 and 2, human-infecting genotypes. In addition, Blastocystis sp., subtypes were clustered within subtypes isolated from humans.</p><p><strong>Conclusion: </strong>This is the first study investigating intestinal protozoa in a super harsh condition. Our findings signify the concern due to the distribution of potentially pathogenic parasites to downstream farmlands through industrial treated wastewaters which are used for irrigation.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148497069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbial remodeling as an ecological strategy for host metabolic management under environmental stress on the Xizang Plateau.","authors":"Cheng Pan, Yangzong Zhaxi, Junru Pan, Cisang Zhuoma, Xiaofeng Luo, Wenrui Ye, Pan Tan, Haiyan Li, Zhenzhen Zhang, Deji Quzong, Duoji Ouzhu, Qiongla, Tianzeng Song, Wangsheng Zhao","doi":"10.1186/s12866-026-05423-8","DOIUrl":"10.1186/s12866-026-05423-8","url":null,"abstract":"<p><p>The adaptive responses of gut bacteria and host metabolism in Xizang sheep to the decline in forage quality and the extremehigh-altitude environment remain largely unclear. This study utilized Xizang sheep as a model, and employed 16S rRNA sequencing, metabolomics, and RT-qPCR to investigate changes in the composition and functional pathways of the gut bacteria, as well as hepatic and muscular metabolism, in response to these stressors. The results indicated that in response to these stressors, the complexity of the gut microbial network in Xizang sheep was reduced, yet the overall positive correlations within the community were enhanced, accompanied by a significant increase in the abundance of Fibrobacterota and Lachnoclostridium, while the abundance of Bacteroides decreased. Furthermore, metabolomic analysis based on gene co-expression network (WGCNA) revealed that the glycerophospholipid metabolism pathway in the liver was suppressed, while the lysine degradation pathway was activated in muscle. These findings reveal the physiological adaptations of the gut bacteria, liver, and muscle of Xizang sheep in response to seasonal variations in grass quality at high altitudes. This research provides a theoretical framework for understanding ruminant adaptation to high-altitude environments.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148469132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Altered microbial cargo in fecal microbiome-derived outer membrane vesicles as novel biomarkers for vascular dementia.","authors":"Xin Li, Wei Wei, Shouchao Wei, Wenwei Xu, Lang Mo, Junjun Wang, He Zhu, Zhou Liu, Fengri Jin","doi":"10.1186/s12866-026-05413-w","DOIUrl":"10.1186/s12866-026-05413-w","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"26 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13379966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148469062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"cAMP-response element binding protein (CREB)-mediated glycolysis enhancement promotes apoptosis of RAW264.7 macrophages infected with BCG under high glucose conditions.","authors":"Yiming Wu, FuYang Song, Gang Zhao, Yujiong Wang","doi":"10.1186/s12866-026-05430-9","DOIUrl":"10.1186/s12866-026-05430-9","url":null,"abstract":"<p><strong>Background: </strong>Macrophages serve as the primary host defense against Mycobacterium tuberculosis (M.tb). Cellular glycolysis plays a critical role in macrophage-mediated resistance to mycobacterial infection. A hyperglycemic microenvironment impairs host defense and exacerbates mycobacterial infection. However, the role of cAMP response element-binding protein (CREB), a key transcription factor regulating metabolism and apoptosis, in macrophages' anti-mycobacterial response under high glucose (HG) conditions remains unclear. Here, we used the attenuated M.tb strain Bacillus Calmette‑Guérin (BCG) to investigate the role of CREB in HG‑modulated glycolysis and apoptosis in infected macrophages.</p><p><strong>Results: </strong>In the present study, we found that BCG infection significantly induced apoptosis and upregulated the expression of key glycolytic enzymes in RAW264.7 macrophages in vitro, and these effects were further amplified under HG conditions. In vivo, C57BL/6J mice fed a high-fat high-sugar (HFHS) diet and infected with BCG showed more pronounced lung tissue inflammatory cell infiltration and bacterial burden, along with upregulated p-CREB, GLUT4 and Cleaved-Caspase 9, compared with infected mice fed normal chow. siRNA-mediated CREB knockdown significantly reduced apoptosis-related proteins and glycolytic enzymes, decreased intracellular ROS accumulation, and improved cell survival in BCG-infected RAW264.7 cells under HG conditions. The glycolysis inhibitor 2-deoxy-D-glucose (2-DG) suppressed glycolytic enzyme expression and apoptosis, with further enhanced protection when combined with CREB siRNA.</p><p><strong>Conclusion: </strong>BCG infection upregulated CREB expression in a time-dependent manner, and this effect was further potentiated under HG conditions. CREB upregulation was accompanied by enhanced glycolysis, increased ROS accumulation, and elevated macrophage apoptosis, and these changes were associated with exacerbated BCG infection under HG conditions. These findings suggest a mechanistic link between hyperglycemia and impaired antimycobacterial defense, and point to CREB as a candidate for further investigation under HG metabolic stress.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13602477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148469071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ramadan fasting-induced modulation of beneficial and pathobiont gut bacteria in a healthy Iranian population.","authors":"Abbas Asoudeh-Fard, Mahmood Nejabat, Kamran Bagheri Lankarani, Mohadeseh Asoudeh-Fard, Ahmad Gholami","doi":"10.1186/s12866-026-05432-7","DOIUrl":"10.1186/s12866-026-05432-7","url":null,"abstract":"<p><strong>Background: </strong>Ramadan fasting induces dietary and metabolic changes that may alter gut microbiota, but species-level effects are not well studied in Middle Eastern populations.</p><p><strong>Methods: </strong>Fecal samples were collected from 30 healthy fasting and 30 non-fasting adults in Shiraz, Iran. Species-level bacterial abundance was quantified using culture-based isolation and SYBR Green qRT-PCR with universal and species-specific primers.</p><p><strong>Results: </strong>Beneficial bacteria-including Bacteroides vulgatus (Ab.352 TH, NCBI PX796484), Bacteroides ovatus (Ab.353 TH, NCBI PX796487), and Odoribacter splanchnicus (Ab.354 TH, NCBI PX796490)-were significantly increased in fasting individuals, while pathobionts such as Prevotella stercorea (Ab.355 TH, NCBI PX796493), Alistipes putredinis (Ab.356 TH, NCBI PX796496), Collinsella aerofaciens (Ab.357 TH, NCBI PX796499), and Enterobacter cloacae (Ab.358 TH, NCBI PX796500) were reduced.</p><p><strong>Conclusion: </strong>Ramadan fasting promotes a gut microbiota enriched in SCFA-producing beneficial bacteria and depleted of inflammation-associated pathobionts, suggesting species-specific microbial adaptations that may enhance gastrointestinal, immunological, and systemic health.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148497028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended lag phases and variable antibiotic tolerance in β-lactam-induced small colony variants of Enterococcus faecalis.","authors":"Lara Thieme, Kamran A Mirza, Mehri Azimi, Nicole Enslinger, Mara Lohde, Mateusz Jundzill, Christian Brandt, Mathias W Pletz, Oliwia Makarewicz","doi":"10.1186/s12866-026-05403-y","DOIUrl":"10.1186/s12866-026-05403-y","url":null,"abstract":"<p><p>Small colony variants (SCVs) of Enterococcus faecalis are associated with persistent infections and have been linked to altered antibiotic susceptibility and tolerance. Here, we investigated SCVs emerging under β-lactam exposure in comparison to corresponding normal colony phenotypes (NCPs). SCVs were generated in vitro under benzylpenicillin, ceftriaxone, and combination therapy and characterized by growth kinetics, antibiotic susceptibility (MIC), killing dynamics (MDK), and bactericidal activity (MBC). Virulence and infection dynamics were further assessed using the Galleria mellonella model. SCVs displayed reduced growth rates and prolonged lag phases, which were partially reversible upon subcultivation. β-Lactam exposure induced substantial MIC increases across multiple lineages, including both SCV and NCP variants, indicating that reduced susceptibility represents a general adaptive response rather than a phenotype-specific trait. In contrast, killing dynamics revealed heterogeneous tolerance profiles that were not consistently associated with SCV formation. While selected SCVs exhibited prolonged MDK values consistent with increased tolerance, other variants showed rapid killing despite elevated MICs. MBC values further supported this dissociation, particularly for ceftriaxone, where bactericidal activity remained limited across variants. In the G. mellonella model, SCVs showed delayed infection onset but did not uniformly result in reduced virulence. Together, these findings indicate that SCVs in E. faecalis represent a heterogeneous and reversible adaptive state. Antibiotic tolerance depends on growth dynamics rather than phenotype alone, while MIC shifts occur independently of SCV formation, highlighting the importance of distinguishing between resistance, tolerance, and phenotypic adaptation.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"26 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13377840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148469087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}