Lichao He, Chao Jiang, He Dong, Yinuo Wang, Jiaxin Tang, Mengjie Hu, Junjie Luo, Shuai Du, Yushan Jia, Yanzi Xiao, Sihan You
{"title":"纤维素酶和植物乳杆菌对高粱秸秆青贮性能和细菌群落的影响。","authors":"Lichao He, Chao Jiang, He Dong, Yinuo Wang, Jiaxin Tang, Mengjie Hu, Junjie Luo, Shuai Du, Yushan Jia, Yanzi Xiao, Sihan You","doi":"10.1186/s12866-025-03982-w","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to evaluate the effects of cellulase or Lactobacillus plantarum (L. plantarum) on the fermentation characteristics and microbial community structure of the sorghum straw silage. Sorghum straw was treated with the following four experimental conditions: distilled water (control, CK), cellulase (CEL), Lactobacillus plantarum (LP), and a combined treatment of Lactobacillus plantarum with cellulase (LPCEL). These results indicated that the LP treatment could markedly (p < 0.05) preserve the crude protein content compared to that in other treatments, whereas the CEL significantly (p < 0.05) reduced the acid detergent fiber content, while the LPCEL had the highest lactic acid content and lowest pH value. Proteobacteria and Pantoea were identified as the dominant phylum and genus in fresh materials, respectively. This phylum level dominance transitioned to Firmicutes post-treatment, while at the genus level, the community shifted from Pantoea to co-dominance of Lactobacillus and Prevotella, with Lactobacillus being the most abundant in both the CEL and LPCEL treatments. In conclusion, adding L. plantarum and cellulase to sorghum straw can significantly improve the fermentation quality of sorghum straw silage, and improve the nutritional value of silage by affecting the microbial community structure and metabolic pathways.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"300"},"PeriodicalIF":4.0000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083020/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effects of cellulase or Lactobacillus plantarum on ensiling performance and bacterial community of sorghum straw.\",\"authors\":\"Lichao He, Chao Jiang, He Dong, Yinuo Wang, Jiaxin Tang, Mengjie Hu, Junjie Luo, Shuai Du, Yushan Jia, Yanzi Xiao, Sihan You\",\"doi\":\"10.1186/s12866-025-03982-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to evaluate the effects of cellulase or Lactobacillus plantarum (L. plantarum) on the fermentation characteristics and microbial community structure of the sorghum straw silage. Sorghum straw was treated with the following four experimental conditions: distilled water (control, CK), cellulase (CEL), Lactobacillus plantarum (LP), and a combined treatment of Lactobacillus plantarum with cellulase (LPCEL). These results indicated that the LP treatment could markedly (p < 0.05) preserve the crude protein content compared to that in other treatments, whereas the CEL significantly (p < 0.05) reduced the acid detergent fiber content, while the LPCEL had the highest lactic acid content and lowest pH value. Proteobacteria and Pantoea were identified as the dominant phylum and genus in fresh materials, respectively. This phylum level dominance transitioned to Firmicutes post-treatment, while at the genus level, the community shifted from Pantoea to co-dominance of Lactobacillus and Prevotella, with Lactobacillus being the most abundant in both the CEL and LPCEL treatments. In conclusion, adding L. plantarum and cellulase to sorghum straw can significantly improve the fermentation quality of sorghum straw silage, and improve the nutritional value of silage by affecting the microbial community structure and metabolic pathways.</p>\",\"PeriodicalId\":9233,\"journal\":{\"name\":\"BMC Microbiology\",\"volume\":\"25 1\",\"pages\":\"300\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083020/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1186/s12866-025-03982-w\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12866-025-03982-w","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Effects of cellulase or Lactobacillus plantarum on ensiling performance and bacterial community of sorghum straw.
This study aimed to evaluate the effects of cellulase or Lactobacillus plantarum (L. plantarum) on the fermentation characteristics and microbial community structure of the sorghum straw silage. Sorghum straw was treated with the following four experimental conditions: distilled water (control, CK), cellulase (CEL), Lactobacillus plantarum (LP), and a combined treatment of Lactobacillus plantarum with cellulase (LPCEL). These results indicated that the LP treatment could markedly (p < 0.05) preserve the crude protein content compared to that in other treatments, whereas the CEL significantly (p < 0.05) reduced the acid detergent fiber content, while the LPCEL had the highest lactic acid content and lowest pH value. Proteobacteria and Pantoea were identified as the dominant phylum and genus in fresh materials, respectively. This phylum level dominance transitioned to Firmicutes post-treatment, while at the genus level, the community shifted from Pantoea to co-dominance of Lactobacillus and Prevotella, with Lactobacillus being the most abundant in both the CEL and LPCEL treatments. In conclusion, adding L. plantarum and cellulase to sorghum straw can significantly improve the fermentation quality of sorghum straw silage, and improve the nutritional value of silage by affecting the microbial community structure and metabolic pathways.
期刊介绍:
BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.