肺炎克雷伯菌对氯霉素异耐药的检测与鉴定。

IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Qihong Kuang, Xiaorui Zhang, Fangping Ou, Lingling Liu, Hui Deng, Bo Yang, Lingxian Yi, Daojin Yu
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引用次数: 0

摘要

背景:异耐药是敏感菌进化为耐药菌株的重要途径,对目前的临床实验室检测方法提出了挑战。目的:研究不同来源肺炎克雷伯菌的耐药性差异,评估氯霉素异源耐药(CHR)的流行情况,探讨其可能的原因及相关关键基因。方法:从不同来源的801份样品中分离出肺炎克雷伯菌,并对其进行抗菌药物敏感性评价。采用改良的Kirby-Bauer圆盘扩散法、种群分析谱法(PAP)和杀菌曲线法鉴定异耐药菌。测定了CHR菌株的生长曲线和稳定性。为了分析影响CHR形成的因素,我们在17个抗性亚群中检测了抗性基因cmlA、cat1和floR,以及毒力基因fimH、wabG、kfu、uge和aerobactin。结果:198株肺炎克雷伯菌对呋喃妥因、四环素和氯霉素的耐药率分别为73.74%、57.58%和51.01%。CHR患病率为8.59%(198例中有17例),显著降低了氯霉素的体外杀菌效果。值得注意的是,76.47%(13/17)的菌株携带cat1和/或floR基因,而毒力基因wabG、fimH、uge和kfu的感染率分别为100%、100%、76.47%和47.06%。结论:floR和/或cat1基因在氯霉素异源耐药机制中起关键作用,毒力基因的存在可能进一步促进CHR的发生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection and characterization of heteroresistance to chloramphenicol in Klebsiella pneumoniae isolates.

Background: Heteroresistance represents a significant pathway through which sensitive bacteria evolve into resistant strains, posing challenges for current clinical laboratory detection methods.

Objectives: This study aimed to investigate the differences in resistance among K. pneumoniae isolates from various sources, assess the prevalence of chloramphenicol heteroresistance (CHR), and explore the potential causes and key genes associated with CHR.

Methods: K. pneumoniae was isolated from 801 samples obtained from various sources, and its susceptibility to antibacterial agents was assessed. The modified Kirby-Bauer disk diffusion method, population analysis profiling (PAP), and bactericidal curve assays were employed to identify heteroresistant bacteria. Additionally, the growth curve and stability of CHR strains were measured. To analyze the factors influencing the formation of CHR, we detected the resistance genes cmlA, cat1, and floR across 17 resistant subpopulations, along with virulence genes such as fimH, wabG, kfu, uge, and aerobactin.

Results: Among the 198 K. pneumoniae tested, resistance rates to nitrofurantoin, tetracycline, and chloramphenicol were found to be 73.74%, 57.58%, and 51.01%, respectively. The prevalence of CHR was determined to be 8.59% (17 out of 198), which significantly diminished the in vitro bactericidal efficacy of chloramphenicol. Notably, 76.47% (13/17) of the isolates harbored the cat1 and/or floR genes, while the prevalence of the virulence genes wabG, fimH, uge, and kfu was 100%, 100%, 76.47%, and 47.06%, respectively.

Conclusion: The floR and/or cat1 genes are pivotal in the mechanism underlying heteroresistance to chloramphenicol, and the presence of virulence genes could further contribute to the development of CHR.

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来源期刊
BMC Microbiology
BMC Microbiology 生物-微生物学
CiteScore
7.20
自引率
0.00%
发文量
280
审稿时长
3 months
期刊介绍: BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.
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