Biology Open最新文献_第7页

The relationship between locomotion and hindlimb morphology in the leopard (Panthera pardus) using a geometric morphometric approach. 用几何形态计量学方法研究豹（Panthera pardus）运动与后肢形态之间的关系。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-24 DOI: 10.1242/bio.061823

Riyanta Naidoo, Safiyyah Iqbal

{"title":"The relationship between locomotion and hindlimb morphology in the leopard (Panthera pardus) using a geometric morphometric approach.","authors":"Riyanta Naidoo, Safiyyah Iqbal","doi":"10.1242/bio.061823","DOIUrl":"10.1242/bio.061823","url":null,"abstract":"Felid bone morphology is highly influenced by factors such as locomotion, body size, and foraging behaviour. Understanding how these factors influence bone morphology is important for interpreting the behaviour and ecology of such species. This study aimed to determine the extent to which Panthera pardus (i.e. leopard) hindlimb morphology differs from that of other Panthera species, particularly Panthera leo (i.e. lion). Landmark-based geometric morphometric analyses were used to compare 27 Panthera femurs in the anterior and posterior views, by the use of principal component analyses. Distinct clusters were found linking the Panthera species for both the anterior and posterior views, inferring a difference in the femur morphology of the species. The Procrustes ANOVA regression further showed a significant difference in the mean shape between the Panthera femurs, for both the anterior and posterior views. A clear relationship was found between femur morphology and body size, with leopards possessing a more gracile and elongated femur to support a smaller body mass and lions possessing a more robust and stunted femur to support a larger body mass. It was found that femur morphology also correlates with locomotive flexibility and hunting success in felids. Leopard femur morphology aids in speed and flexibility during hunting, as well as aids in propulsion that allows for arboreal locomotion. It was ultimately deduced that femur morphology differs between Panthera species, according to their mechanical demands during locomotion.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 12","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of nitric oxide in Octopus maya nervous system and its potential role in sensory perception. 章鱼玛雅神经系统中一氧化氮的特征及其在感官知觉中的潜在作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-11-28 DOI: 10.1242/bio.061756

Fabián Vergara-Ovalle, Martha León-Olea, Eduardo Sánchez-Islas, Francisco Pellicer

引用次数: 0

Constructing a doxycycline-inducible system for an epithelial-to-mesenchymal transition model in MCF10A cells. 构建强力霉素诱导的MCF10A细胞上皮-间质转化模型。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-09 DOI: 10.1242/bio.061790

Yaxuan Sun, Xun Zhou, Xiaohui Hu

{"title":"Constructing a doxycycline-inducible system for an epithelial-to-mesenchymal transition model in MCF10A cells.","authors":"Yaxuan Sun, Xun Zhou, Xiaohui Hu","doi":"10.1242/bio.061790","DOIUrl":"10.1242/bio.061790","url":null,"abstract":"Epithelial to mesenchymal transition (EMT) has been shown to play an essential role in the early stages of cancer cell invasion and metastasis. Inducible EMT models can initiate EMT in a controlled manner, thereby providing the opportunity to determine whether a cancer-associated gene influences cancer metastasis by triggering EMT. Moreover, different inducible EMT models enable the investigation of specific mechanisms of EMT modulation by various genes, facilitating a more precise understanding of how these genes influence cancer metastasis through the induction of EMT. Unfortunately, current inducible EMT models still present unmet needs. Therefore, we aimed to establish an inducible EMT model in MCF10A cells, a spontaneously immortalized human fibrocystic mammary cell line, by manipulating the expression of mouse Twist1 (mTwist1). In this study, we first compared the EMT induction capacity between human TWIST1 (hTWIST1) and mTwist1, and selected mTwist1 for further investigation. By monitoring the changes in epithelial and mesenchymal markers at different induction time points, we examined the EMT process in both polyclonal and monoclonal MCF10A cells that express doxycycline (DOX)-inducible mTwist1. Furthermore, our results showed that doxycycline-induced mTwist1 expression triggered EMT at a similar rate to TGFβ1-induced EMT in MCF10A cells. Additionally, this process was reversible upon DOX withdrawal. Thus, we have established a robust inducible EMT model in MCF10A cells, which can be used to further study cancer metastasis-driving genes.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 12","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of lin-12 notch in C. elegans anchor cell proliferation. in-12缺口在线虫锚定细胞增殖中的作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-30 DOI: 10.1242/bio.061816

Alex Hajnal, Ting Deng, Evelyn Lattmann

{"title":"The role of lin-12 notch in C. elegans anchor cell proliferation.","authors":"Alex Hajnal, Ting Deng, Evelyn Lattmann","doi":"10.1242/bio.061816","DOIUrl":"10.1242/bio.061816","url":null,"abstract":"The gonadal anchor cell (AC) is an essential organizer for the development of the egg-laying organ in the C. elegans hermaphrodite. Recent work has investigated the mechanisms that control the quiescent state the AC adopts while fulfilling its functions. In this context, the transcription factors EGL-43 and NHR-67 are required to maintain the G1 cell cycle arrest of the AC and prevent proliferation. While NHR-67 acts primarily by up-regulating the CDK inhibitor CKI-1, the role of EGL-43 in this process has been subject to debate. Deng et al. (2020) reported that inhibition of the notch receptor lin-12 by RNAi partially suppressed the AC proliferation phenotype caused by egl-43 RNAi. By contrast, Martinez et al. (2022) found that down-regulation of LIN-12 NOTCH via the auxin-inducible degradation system did not reduce AC proliferation. To resolve this issue, we performed egl-43 RNAi in the background of a lin-12 null allele and observed a similar suppression of AC proliferation as reported previously by Deng et al. (2020). Hence, AC proliferation caused by the downregulation of egl-43 partially depends on LIN-12 NOTCH signaling.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 12","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defensive tactics: lessons from Drosophila. 防御战术：来自果蝇的教训。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-24 DOI: 10.1242/bio.061609

Madhumala K Sadanandappa, Subhana Ahmad, Robinson Mohanraj, Mrunal Ratnaparkhi, Shivaprasad H Sathyanarayana

引用次数: 0

Progressive Palaeontology 2024 conference report. 进步古生物学2024年会议报告。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-06 DOI: 10.1242/bio.061822

Hady George, Thomas Farrell, James R G Rawson, Isaura Aguilar-Pedrayes, Benton Walters, Kirsten E Flett

引用次数: 0

Identification of a postnatal period of interdependent neurogenesis and apoptosis in peripheral neurons. 确定外周神经元的神经发生和凋亡相互依存的出生后时期。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-11-15 Epub Date: 2024-11-11 DOI: 10.1242/bio.060541

Catherine L Kaminski, Debarghya Dutta Banik, Ligia B Schmitd, Brian A Pierchala

{"title":"Identification of a postnatal period of interdependent neurogenesis and apoptosis in peripheral neurons.","authors":"Catherine L Kaminski, Debarghya Dutta Banik, Ligia B Schmitd, Brian A Pierchala","doi":"10.1242/bio.060541","DOIUrl":"10.1242/bio.060541","url":null,"abstract":"During neurogenesis, excessive numbers of neurons are produced in most regions of the central and peripheral nervous systems. Nonessential neurons are eliminated by apoptosis, or programmed cell death. This has been most thoroughly characterized in the peripheral nervous system (PNS) where targets of innervation play a key role in this process. As maturing neurons project axons towards their targets of innervation, they become dependent upon these targets for survival. Survival factors, also called neurotrophic factors, are produced by targets, inhibit apoptosis cascades, and promote further growth and differentiation. Because neurotrophic factors are limited, as is target size, neurons that do not correctly and efficiently innervate targets undergo apoptosis ( Levi-Montalcini, 1987; Davies, 1996). Thus, excessive neurogenesis acts to ensure that sufficient numbers of neurons are produced during development. In the superior cervical ganglion (SCG), this process of neurogenesis and subsequent apoptosis is reported to be complete by postnatal day 3-4 (P3-P4) in mice. Surprisingly, we observed significant numbers of apoptotic neurons out to P14, and neurogenesis was still present at P14 as well. In both the SCG and geniculate ganglion (GG), postnatal neurogenesis was dependent on apoptosis because little or no postnatal neurogenesis was observed in Bax-/- mice, in which apoptosis is eliminated. These results indicate that both neurogenesis and apoptosis continue to occur well after birth in peripheral ganglia, and that neurogenesis depends on apoptosis, suggesting that neurogenesis continues postnatally to replace neurons that are eliminated during synaptic refinement.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reliable replicative lifespan determination of yeast with a single-channel microfluidic chip. 利用单通道微流控芯片可靠测定酵母的复制寿命

IF 1.8 4区生物学

Biology Open Pub Date : 2024-11-15 Epub Date: 2024-11-26 DOI: 10.1242/bio.060596

Valentina Salzman, Moises R Bustamante Torres, Francisco G Correa Tedesco, Nahuel Tarkowski, María J Godás Willems, Joaquín N Bravo, Magalí Mercuri, Dante G Mercado, Guido Berlin, Martín G Bellino, Pablo S Aguilar, Laura C Estrada

{"title":"Reliable replicative lifespan determination of yeast with a single-channel microfluidic chip.","authors":"Valentina Salzman, Moises R Bustamante Torres, Francisco G Correa Tedesco, Nahuel Tarkowski, María J Godás Willems, Joaquín N Bravo, Magalí Mercuri, Dante G Mercado, Guido Berlin, Martín G Bellino, Pablo S Aguilar, Laura C Estrada","doi":"10.1242/bio.060596","DOIUrl":"10.1242/bio.060596","url":null,"abstract":"Saccharomyces cerevisiae is a powerful model for aging research due to its short lifespan and genetic malleability. Microfluidic devices offer an attractive approach enabling rapid monitoring of hundreds of cells during their entire replicative lifespan (RLS). Yet, key operational issues such as contaminations, cell loss, and cell-aggregates-dependent flow obstruction can hinder RLS experiments. We report the development of a microfluidic device configuration that effectively prevents flow blockage. We conducted comprehensive performance characterization, evaluating trapping efficiency, cell retention, budding orientation, and cell aggregate formation. The optimized device successfully supported long-term culturing and reliable RLS measurements of budding yeast strains. For accurate lifespan determination, a detailed workflow is provided that includes device fabrication, live microscopy setup, and characterization of cell age distribution. This work describes an accessible and reliable microfluidic device for yeast RLS studies, promoting further exploration in aging research.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular dynamics of ubiquitin-like 3 visualized using an inducible fluorescent timer expression system. 利用可诱导荧光定时器表达系统观察泛素样 3 的胞内动态。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-11-15 Epub Date: 2024-11-05 DOI: 10.1242/bio.060345

Yuka Terada, Kumi Obara, Yusuke Yoshioka, Takahiro Ochiya, Haruhiko Bito, Kunihiro Tsuchida, Hiroshi Ageta, Natsumi Ageta-Ishihara

{"title":"Intracellular dynamics of ubiquitin-like 3 visualized using an inducible fluorescent timer expression system.","authors":"Yuka Terada, Kumi Obara, Yusuke Yoshioka, Takahiro Ochiya, Haruhiko Bito, Kunihiro Tsuchida, Hiroshi Ageta, Natsumi Ageta-Ishihara","doi":"10.1242/bio.060345","DOIUrl":"10.1242/bio.060345","url":null,"abstract":"Exosomes are small extracellular vesicles (sEVs) secreted via multivesicular bodies (MVBs)/late endosomes and mediators of cell-cell communication. We previously reported a novel post-translational modification by ubiquitin-like 3 (UBL3). UBL3 is localized in MVBs and the plasma membrane and released outside as sEVs, including exosomes. Approximately 60% of proteins sorted in sEVs are affected by UBL3 and localized in various organelles, the plasma membrane, and the cytosol, suggesting that its dynamic movement in the cell before entering the MVBs. To examine the intracellular dynamics of UBL3, we constructed a sophisticated visualization system via fusing fluorescent timers that changed from blue to red form over time with UBL3 and by its expression under Tet-on regulation. Intriguingly, we found that after synthesis, UBL3 was initially distributed within the cytosol. Subsequently, UBL3 was localized to MVBs and the plasma membrane and finally showed predominant accumulation in MVBs. Furthermore, by super-resolution microscopy analysis, UBL3 was found to be associated with one of its substrates, α-tubulin, in the cytosol, and the complex was subsequently transported to MVBs. This spatiotemporal visualization system for UBL3 will form a basis for further studies to elucidate when and where UBL3 associates with its substrates/binding proteins before localization in MVBs.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feeding a rich diet supplemented with the translation inhibitor cycloheximide decreases lifespan and ovary size in Drosophila. 喂食富含翻译抑制剂环己亚胺的食物会降低果蝇的寿命和卵巢大小。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-11-15 Epub Date: 2024-11-25 DOI: 10.1242/bio.061697

Hye Jin Hwang, Rachel T Cox

{"title":"Feeding a rich diet supplemented with the translation inhibitor cycloheximide decreases lifespan and ovary size in Drosophila.","authors":"Hye Jin Hwang, Rachel T Cox","doi":"10.1242/bio.061697","DOIUrl":"10.1242/bio.061697","url":null,"abstract":"Drosophila oogenesis has long been an important model for understanding myriad cellular processes controlling development, RNA biology and patterning. Flies are easily fed drugs to disrupt various molecular pathways. However, this is often done under poor nutrient conditions that adversely affect oogenesis, thus making analysis challenging. Cycloheximide is a widely used compound that binds to and stalls the ribosome, therefore reducing protein synthesis. As egg production is a highly nutrient-dependent process, we developed a method to feed female Drosophila a rich diet of yeast paste supplemented with cycloheximide to better determine the effect of cycloheximide treatment on oogenesis. We found that flies readily consumed cycloheximide-supplemented yeast paste. Males and females had reduced lifespans when maintained on cycloheximide, with males exhibiting a dose-dependent decrease. Although females did not exhibit decreased egg laying, their ovaries were smaller and the number of progeny reduced, indicating substandard egg quality. Finally, females fed cycloheximide had disrupted oogenesis, with smaller ovaries, missing ovariole stages, and an increase in apoptotic follicles. Together, these data support that reduced protein synthesis adversely affects oogenesis with a rich diet that provides optimal nutrient conditions. In addition, this method could be used more broadly to test the effect of other drugs on Drosophila oogenesis without the confounding effects caused by poor nutrition.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0