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Shared and unique consequences of Joubert Syndrome gene dysfunction on the zebrafish central nervous system. 朱伯特综合征基因功能失调对斑马鱼中枢神经系统的共同和独特影响
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-11-15 Epub Date: 2024-11-12 DOI: 10.1242/bio.060421
Alexandra R Noble, Markus Masek, Claudia Hofmann, Arianna Cuoco, Tamara D S Rusterholz, Hayriye Özkoc, Nadja R Greter, Ian G Phelps, Nikita Vladimirov, Sepp Kollmorgen, Esther Stoeckli, Ruxandra Bachmann-Gagescu
{"title":"Shared and unique consequences of Joubert Syndrome gene dysfunction on the zebrafish central nervous system.","authors":"Alexandra R Noble, Markus Masek, Claudia Hofmann, Arianna Cuoco, Tamara D S Rusterholz, Hayriye Özkoc, Nadja R Greter, Ian G Phelps, Nikita Vladimirov, Sepp Kollmorgen, Esther Stoeckli, Ruxandra Bachmann-Gagescu","doi":"10.1242/bio.060421","DOIUrl":"10.1242/bio.060421","url":null,"abstract":"<p><p>Joubert Syndrome (JBTS) is a neurodevelopmental ciliopathy defined by a highly specific midbrain-hindbrain malformation, variably associated with additional neurological features. JBTS displays prominent genetic heterogeneity with >40 causative genes that encode proteins localising to the primary cilium, a sensory organelle that is essential for transduction of signalling pathways during neurodevelopment, among other vital functions. JBTS proteins localise to distinct ciliary subcompartments, suggesting diverse functions in cilium biology. Currently, there is no unifying pathomechanism to explain how dysfunction of such diverse primary cilia-related proteins results in such a highly specific brain abnormality. To identify the shared consequence of JBTS gene dysfunction, we carried out transcriptomic analysis using zebrafish mutants for the JBTS-causative genes cc2d2aw38, cep290fh297, inpp5ezh506, talpid3i264 and togaram1zh510 and the Bardet-Biedl syndrome-causative gene bbs1k742. We identified no commonly dysregulated signalling pathways in these mutants and yet all mutants displayed an enrichment of altered gene sets related to central nervous system function. We found that JBTS mutants have altered primary cilia throughout the brain but do not display abnormal brain morphology. Nonetheless, behavioural analyses revealed reduced locomotion and loss of postural control which, together with the transcriptomic results, hint at underlying abnormalities in neuronal activity and/or neuronal circuit function. These zebrafish models therefore offer the unique opportunity to study the role of primary cilia in neuronal function beyond early patterning, proliferation and differentiation.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Response to 'Commentary on Thoral et al. (2024) The relationship between mitochondrial respiration, resting metabolic rate and blood cell count in great tits'. 对 "Thoral 等人(2024 年)关于大山雀线粒体呼吸、静息代谢率和血细胞计数之间关系的评论 "的回应。
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-11-15 Epub Date: 2024-11-20 DOI: 10.1242/bio.061771
Elisa Thoral, Carmen C García-Díaz, Elin Persson, Imen Chamkha, Eskil Elmér, Suvi Ruuskanen, Andreas Nord
{"title":"Response to 'Commentary on Thoral et al. (2024) The relationship between mitochondrial respiration, resting metabolic rate and blood cell count in great tits'.","authors":"Elisa Thoral, Carmen C García-Díaz, Elin Persson, Imen Chamkha, Eskil Elmér, Suvi Ruuskanen, Andreas Nord","doi":"10.1242/bio.061771","DOIUrl":"10.1242/bio.061771","url":null,"abstract":"","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11603115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunofluorescent detection of protein CoAlation in mammalian cells under oxidative stress. 免疫荧光检测氧化应激下哺乳动物细胞中的蛋白质 CoAlation。
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-10-15 Epub Date: 2024-09-30 DOI: 10.1242/bio.061685
Oksana Malanchuk, Antonina Khoruzhenko, Viktoriia Kosach, Anna Bdzhola, Dariy Bidiuk, Charlie Brett, Ivan Gout, Valeriy Filonenko
{"title":"Immunofluorescent detection of protein CoAlation in mammalian cells under oxidative stress.","authors":"Oksana Malanchuk, Antonina Khoruzhenko, Viktoriia Kosach, Anna Bdzhola, Dariy Bidiuk, Charlie Brett, Ivan Gout, Valeriy Filonenko","doi":"10.1242/bio.061685","DOIUrl":"10.1242/bio.061685","url":null,"abstract":"<p><p>Previously, we reported the generation and characterisation of highly specific anti-CoA monoclonal antibodies capable of recognizing CoA in various immunological assays. Utilizing these antibodies in conjunction with mass spectrometry, we identified a wide array of cellular proteins modified by CoA in bacteria and mammalian cells. Furthermore, our findings demonstrated that such modifications could be induced by oxidative or metabolic stress. This study advances the utility of anti-CoA monoclonal antibodies in analysing protein CoAlation, highlighting their effectiveness in immunofluorescent assay. Our data corroborates a significant increase in cellular protein CoAlation induced by oxidative agents. Additionally, we observed that hydrogen-peroxide induced protein CoAlation is predominantly associated with mitochondrial proteins.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11463958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An evaluation of thermal tolerance in six tardigrade species in an active and dry state. 对六种活动和干燥状态下的沙蜥耐热性进行评估。
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-10-15 Epub Date: 2024-09-25 DOI: 10.1242/bio.060485
Jacob Loeffelholz, Emma Meese, Ilaria Giovannini, Karsyn Ullibarri, Sogol Momeni, Nicholas Merfeld, Jessica Wessel, Roberto Guidetti, Lorena Rebecchi, Thomas C Boothby
{"title":"An evaluation of thermal tolerance in six tardigrade species in an active and dry state.","authors":"Jacob Loeffelholz, Emma Meese, Ilaria Giovannini, Karsyn Ullibarri, Sogol Momeni, Nicholas Merfeld, Jessica Wessel, Roberto Guidetti, Lorena Rebecchi, Thomas C Boothby","doi":"10.1242/bio.060485","DOIUrl":"10.1242/bio.060485","url":null,"abstract":"<p><p>Tardigrades are known for their ability to survive extreme conditions. Reports indicate that tardigrade thermal tolerance is enhanced in the desiccated state; however, these reports have almost always used a single tardigrade species and drying/heating methods vary between studies. Using six different species of tardigrades we confirm that desiccation enhances thermal tolerance in tardigrades. Furthermore, we show that differences in thermal tolerance exist between tardigrade species both when hydrated and desiccated. While Viridiscus viridianus survives the highest temperatures in the hydrated state of any species tested here, under hydrated conditions, the thermal tolerance of V. viridianus is restricted to an acute transient stress. Furthermore, unlike other stresses, such as desiccation, where mild initial exposure preconditions some species to survive subsequent harsher treatment, for V. viridianus exposure to mild thermal stress in the hydrated state does not confer protection to harsher heating. Our results suggest that while tardigrades have the capacity to tolerate mild thermal stress while hydrated, survival of high temperatures in a desiccated state is a by-product of tardigrades' ability to survive desiccation.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11451804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Year at the Forefront of Streptophyte Algal Evolution. 链藻进化前沿的一年。
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-19 DOI: 10.1242/bio.061673
Alexander M C Bowles
{"title":"A Year at the Forefront of Streptophyte Algal Evolution.","authors":"Alexander M C Bowles","doi":"10.1242/bio.061673","DOIUrl":"10.1242/bio.061673","url":null,"abstract":"<p><p>Land plants originated from an algal ancestor ∼500 million years ago in one of the most important evolutionary events for life on Earth. Extant streptophyte algae, their closest living relatives, have subsequently received much attention to better understand this major evolutionary transition. Streptophyte algae occupy many different environments, have diverse genomes and display contrasting morphologies (e.g. unicellular, filamentous, three-dimensional). This has historically made inferring these evolutionary events challenging. This A Year at the Forefront Review focusses on research published between July 2023 and June 2024 and intends to provide a short overview of recent discoveries, innovations, resources, and hypotheses regarding streptophyte algal evolution. This work has provided mechanistic insights into ancient evolutionary events that prefigured the origin of land plants and raises new questions for future research into streptophyte algae.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An atlas of Brachypodium distachyon lateral root development. Brachypodium distachyon侧根发育图集。
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-02 DOI: 10.1242/bio.060531
Cristovāo de Jesus Vieira Teixeira, Kevin Bellande, Alja van der Schuren, Devin O'Connor, Christian S Hardtke, Joop E M Vermeer
{"title":"An atlas of Brachypodium distachyon lateral root development.","authors":"Cristovāo de Jesus Vieira Teixeira, Kevin Bellande, Alja van der Schuren, Devin O'Connor, Christian S Hardtke, Joop E M Vermeer","doi":"10.1242/bio.060531","DOIUrl":"10.1242/bio.060531","url":null,"abstract":"<p><p>The root system of plants is a vital part for successful development and adaptation to different soil types and environments. A major determinant of the shape of a plant root system is the formation of lateral roots, allowing for expansion of the root system. Arabidopsis thaliana, with its simple root anatomy, has been extensively studied to reveal the genetic program underlying root branching. However, to get a more general understanding of lateral root development, comparative studies in species with a more complex root anatomy are required. Here, by combining optimized clearing methods and histology, we describe an atlas of lateral root development in Brachypodium distachyon, a wild, temperate grass species. We show that lateral roots initiate from enlarged phloem pole pericycle cells and that the overlying endodermis reactivates its cell cycle and eventually forms the root cap. In addition, auxin signaling reported by the DR5 reporter was not detected in the phloem pole pericycle cells or young primordia. In contrast, auxin signaling was activated in the overlying cortical cell layers, including the exodermis. Thus, Brachypodium is a valuable model to investigate how signaling pathways and cellular responses have been repurposed to facilitate lateral root organogenesis.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of potential TAK1/Map3k7 phosphorylation targets in hypertrophy and cachexia models of skeletal muscle. 骨骼肌肥大和恶病质模型中潜在的 TAK1/Map3k7 磷酸化靶标分析
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-25 DOI: 10.1242/bio.060487
Fatemeh Nasehi, Cameron Rylance, Erin Schnell, Maslyn Ann Greene, Caroline Conway, Zachary Hough, Susan Duckett, Robin C Muise-Helmericks, Ann Catherine Foley
{"title":"Analysis of potential TAK1/Map3k7 phosphorylation targets in hypertrophy and cachexia models of skeletal muscle.","authors":"Fatemeh Nasehi, Cameron Rylance, Erin Schnell, Maslyn Ann Greene, Caroline Conway, Zachary Hough, Susan Duckett, Robin C Muise-Helmericks, Ann Catherine Foley","doi":"10.1242/bio.060487","DOIUrl":"10.1242/bio.060487","url":null,"abstract":"<p><p>TGFβ-activated kinase-1 (TAK1) is phosphorylated during both muscle growth and muscle wasting. To understand how this can lead to such opposite effects, we first performed multiplex kinase array of mouse embryonic stem cells with and without stimulation of TAK1 to determine its potential downstream targets. The phosphorylation of these targets was then compared in three different models: hypertrophic longissimus muscle of Texel sheep, tibialis anterior muscle of mice with cancer-induced cachexia and C2C12-derived myofibers, with and without blockade of TAK1 phosphorylation. In both Texel sheep and in cancer-induced cachexia, phosphorylation of both TAK1 and p38 was increased. Whereas p90RSK was increased in Texel sheep but not cachexia and the phosphorylation of HSP27 and total Jnk were increased in cachexia but not Texel. To understand this further, we examined the expression of these proteins in C2C12 cells as they differentiated into myotubes, with and without blockade of TAK1 phosphorylation. In C2C12 cells, decreased phosphorylation of TAK1 leads to reduced phosphorylation of p38, JNK, and HSP27 after 16 h and muscle fiber hypertrophy after 3 days. However, continuous blockade of this pathway leads to muscle fiber failure, suggesting that the timing of TAK1 activation controls the expression of context-dependent targets.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel technique for atraumatic transurethral catheterisation of male rats. 雄性大鼠无创伤经尿道导尿的新技术。
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-09-15 Epub Date: 2024-08-30 DOI: 10.1242/bio.060476
Steven Liben Zhang, Allen Wei-Jiat Wong
{"title":"A novel technique for atraumatic transurethral catheterisation of male rats.","authors":"Steven Liben Zhang, Allen Wei-Jiat Wong","doi":"10.1242/bio.060476","DOIUrl":"10.1242/bio.060476","url":null,"abstract":"<p><p>Transurethral catheterisation of male rats is technically difficult owing to anatomical peculiarities. In the male rat, the urethral striated sphincter consists of two lateral fascicles separated by an anterior and a posterior strip of connective tissue, which impedes the smooth insertion of a urinary catheter. For rat studies requiring continuous collection of urine, bladder irrigation, or measurement of bladder pressure, investigators either have to exclude the male population (be limited to the female population) or perform percutaneous (suprapubic) bladder puncture in male rats, which is more traumatic and invasive than transurethral catheterisation. This paper describes a novel, atraumatic method of transurethral catheterisation in the male rat, with the aid of a microscope and microsurgical instruments. Six Wistar rats were used for this experiment, all of which were catheterised successfully, with no evidence of bladder or urethral injury. The study shows that male rats can be safely catheterised via the urethra with the aid of a microscope and microsurgical instruments for both visual and tactile feedback. This is a relatively straightforward technique to learn and can allow for inclusion of male rats in future studies requiring urinary analysis or bladder irrigation, without the need for traumatic percutaneous (suprapubic) bladder puncture.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11381925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamics of BMP signaling and stable gene expression in the early Drosophila embryo. 果蝇早期胚胎中 BMP 信号传递和稳定基因表达的动态变化
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-09-15 Epub Date: 2024-08-29 DOI: 10.1242/bio.061646
Hadel Al Asafen, Aydin Beseli, Hung-Yuan Chen, Sharva Hiremath, Cranos M Williams, Gregory T Reeves
{"title":"Dynamics of BMP signaling and stable gene expression in the early Drosophila embryo.","authors":"Hadel Al Asafen, Aydin Beseli, Hung-Yuan Chen, Sharva Hiremath, Cranos M Williams, Gregory T Reeves","doi":"10.1242/bio.061646","DOIUrl":"10.1242/bio.061646","url":null,"abstract":"<p><p>In developing tissues, morphogen gradients are thought to initialize gene expression patterns. However, the relationship between the dynamics of morphogen-encoded signals and gene expression decisions is largely unknown. Here we examine the dynamics of the Bone Morphogenetic Protein (BMP) pathway in Drosophila blastoderm-stage embryos. In this tissue, the BMP pathway is highly dynamic: it begins as a broad and weak signal on the dorsal half of the embryo, then 20-30 min later refines into a narrow, intense peak centered on the dorsal midline. This dynamical progression of the BMP signal raises questions of how it stably activates target genes. Therefore, we performed live imaging of the BMP signal and found that dorsal-lateral cells experience only a short transient in BMP signaling, after which the signal is lost completely. Moreover, we measured the transcriptional response of the BMP target gene pannier in live embryos and found it to remain activated in dorsal-lateral cells, even after the BMP signal is lost. Our findings may suggest that the BMP pathway activates a memory, or 'ratchet' mechanism that may sustain gene expression.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11381920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase. 通过 CRISPR-Cas9 和 phiC31 整合酶实现瞬时可视基因分型和便捷的特定位点转基因。
IF 1.8 4区 生物学
Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-03 DOI: 10.1242/bio.061666
Junyan Ma, Weiting Zhang, Simin Rahimialiabadi, Nikkitha Umesh Ganesh, Zhengwang Sun, Saba Parvez, Randall T Peterson, Jing-Ruey Joanna Yeh
{"title":"Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase.","authors":"Junyan Ma, Weiting Zhang, Simin Rahimialiabadi, Nikkitha Umesh Ganesh, Zhengwang Sun, Saba Parvez, Randall T Peterson, Jing-Ruey Joanna Yeh","doi":"10.1242/bio.061666","DOIUrl":"10.1242/bio.061666","url":null,"abstract":"<p><p>Here, we introduce 'TICIT', targeted integration by CRISPR-Cas9 and integrase technologies, which utilizes the site-specific DNA recombinase - phiC31 integrase - to insert large DNA fragments into CRISPR-Cas9 target loci. This technique, which relies on first knocking in a 39-basepair phiC31 landing site via CRISPR-Cas9, enables researchers to repeatedly perform site-specific transgenesis at the exact genomic location with high precision and efficiency. We applied this approach to devise a method for the instantaneous determination of a zebrafish's genotype simply by examining its color. When a zebrafish mutant line must be propagated as heterozygotes due to homozygous lethality, employing this method allows facile identification of a population of homozygous mutant embryos even before the mutant phenotypes manifest. Thus, it should facilitate various downstream applications, such as large-scale chemical screens. We demonstrated that TICIT could also create reporter fish driven by an endogenous promoter. Further, we identified a landing site in the tyrosinase gene that could support transgene expression in a broad spectrum of tissue and cell types. In sum, TICIT enables site-specific DNA integration without requiring complex donor DNA construction. It can yield consistent transgene expression, facilitate diverse applications in zebrafish, and may be applicable to cells in culture and other model organisms.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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