{"title":"Aging-related behavioral defects in Caenorhabditis elegans.","authors":"Jingxuan Zeng, Chung-Kuan Chen, Yuichi Iino, Yu Toyoshima","doi":"10.1242/bio.062711","DOIUrl":"10.1242/bio.062711","url":null,"abstract":"<p><p>With the rapid development of quantitative behavioral analysis, locomotion behaviors can be examined and described in greater detail. However, behavioral structures underlying the dysfunctions and locomotor defects observed with aging remain poorly understood. Here, we recorded and analyzed worms' locomotion movement for both young and aged individuals. The results revealed distinct locomotion patterns between the two groups. Compared to the young worms, the aged individuals exhibited more disorganized forward locomotion, characterized by reduced periodicity and lower cycle stability. The time duration and frequency of backward locomotion did not differ significantly between the two groups, whereas turn behavior following reversals was significantly reduced. Moreover, the aged worms showed increased individual heterogeneity in the usage of behavioral repertoire with age. In summary, this study systematically characterizes the behavior defects of aged animals by quantifying changes in locomotor states, event transitions, and behavior organization.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13552477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology OpenPub Date : 2026-08-15Epub Date: 2026-08-27DOI: 10.1242/bio.062621
Keegan Lutek, Alyssa Y Stark
{"title":"The effect of strain rate on adhesion in the purple sea urchin (Strongylocentrotus purpuratus).","authors":"Keegan Lutek, Alyssa Y Stark","doi":"10.1242/bio.062621","DOIUrl":"10.1242/bio.062621","url":null,"abstract":"<p><p>Sea urchins attach to intertidal substrates in the face of dynamic hydrodynamic forces using flexible, adhesive tube feet. Tube-foot-based adhesion varies between species and among populations with different habitats (i.e. temperature and hydrodynamic forces). While some sea urchin species have tube foot stem tissues (tested in anesthetized individuals) that better resist breaking under higher loading rates, it remains unclear whether this improves the whole-animal adhesive performance in unanesthetized animals. To test whether the loading rate and previous hydrodynamic exposure influence the adhesive performance of the intertidal purple sea urchin, Strongylocentrotus purpuratus, we measured the strain rate dependence of the tube foot disc, tube foot stem, and whole-animal adhesion, using three sets of sea urchins with different environmental histories. In unanesthetized S. purpuratus, strain rate influenced tube foot disc tenacity, but not the tube foot stem or whole-animal adhesion. Rather, sea urchins collected more recently had higher whole-animal adhesion, and reliance on the tube foot disc and the tube foot stem varied among animal sets. These results further support the hypothesis that hydrodynamic loading maintains adhesive performance in sea urchins and highlight the importance of investigating strain rate at levels of organization that impact organismal performance in situ.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13580561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology OpenPub Date : 2026-08-15Epub Date: 2026-08-07DOI: 10.1242/bio.061700
Renaud Balthazard, William Mitchell, Maxime Raymond, Arnau Ballestero Vidal, Dominic G Roy, Libia Cecilia Palma Zambrano, Mohamed Abdelwafi Moulefera, Denis Faubert, Sarah Pasquin, Jean-François Gauchat, Jacques Thibodeau
{"title":"A proximity ligation screen identifies SNAT2 as a novel target of the MARCH1 E3 ubiquitin ligase.","authors":"Renaud Balthazard, William Mitchell, Maxime Raymond, Arnau Ballestero Vidal, Dominic G Roy, Libia Cecilia Palma Zambrano, Mohamed Abdelwafi Moulefera, Denis Faubert, Sarah Pasquin, Jean-François Gauchat, Jacques Thibodeau","doi":"10.1242/bio.061700","DOIUrl":"10.1242/bio.061700","url":null,"abstract":"<p><p>E3 ubiquitin ligases include hundreds of members that can regulate the half-life of other proteins but also modulate their cellular localization and functions. While MARCH1 appears to target principally immune cell components, such as MHC class II molecules and the co-stimulatory molecule CD86, the repertory of its targets remains to be fully documented. Here, we adapted a proximity-dependent biotin identification (BioID)-based screening approach in live HEK293 cells. We transfected a fusion protein consisting of mouse MARCH1 linked to YFP at its N-terminus and to the biotin ligase of Aquifex aeolicus at its C-terminus. Upon transient overexpression in the presence of biotin, we could recover biotinylated proteins that are presumably found within 10 nm of MARCH1. CD98 and CD71, two previously described targets of MARCH1, were identified. Of 16 other biotinylated proteins identified by semi-quantitative mass spectrometry, ten were tested directly by flow cytometry to monitor their expression in the presence or absence of transfected MARCH1. SNAT2 was particularly sensitive to the presence of MARCH1 and was found to be ubiquitinated on western blots. Thus, BioID2 is an effective mean of characterizing the interactome of MARCH1, and the identification of SNAT2 suggests a role of this ubiquitin ligase in cellular metabolism.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13488409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology OpenPub Date : 2026-08-15Epub Date: 2026-08-14DOI: 10.1242/bio.062602
Abigail Thuringer, Elizabeth Steinmetz, Muna Abdullahi, Afshan Ismat
{"title":"The extracellular protease AdamTS-B is a dosage-sensitive regulator of unicellular tracheal branch morphogenesis.","authors":"Abigail Thuringer, Elizabeth Steinmetz, Muna Abdullahi, Afshan Ismat","doi":"10.1242/bio.062602","DOIUrl":"10.1242/bio.062602","url":null,"abstract":"<p><p>Epithelial tube morphogenesis requires coordinated interactions between epithelial cells and their extracellular environment, yet the extracellular mechanisms that regulate this process remain poorly understood. The Drosophila trachea provides an excellent model for investigating how extracellular remodeling contributes to epithelial tube morphogenesis. AdamTS-B, an extracellular ADAMTS protease, is expressed in the embryonic trachea from early to late stages of tracheal development. Loss of AdamTS-B caused mild defects in unicellular branch organization, whereas overexpression produced ectopic cyst-like structures. Additionally, AdamTS-B localized intracellularly and to basal protrusions of tracheal cells. Constitutively active EGFR phenocopied the AdamTS-B overexpression phenotype. Together, our findings identify AdamTS-B as a dosage-sensitive regulator of unicellular tracheal branch morphogenesis and support a model in which extracellular proteolysis contributes to epithelial tube architecture. The phenotypic similarity between AdamTS-B overexpression and constitutive EGFR activation further suggests a potential functional relationship between extracellular proteolysis and EGFR signaling during tracheal development.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13552476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology OpenPub Date : 2026-07-15Epub Date: 2026-07-17DOI: 10.1242/bio.062747
Cristina Gildee, Patricia Ann Kramer
{"title":"Testing the limits: the impact of extreme functional hip width on the muscular demands of walking.","authors":"Cristina Gildee, Patricia Ann Kramer","doi":"10.1242/bio.062747","DOIUrl":"10.1242/bio.062747","url":null,"abstract":"<p><p>The obstetrical dilemma (OD) posits that hominin pelvic morphology reflects a trade-off between obstetric demands and efficient bipedal locomotion - wider pelves facilitate childbirth but increase mechanical demands on stabilizing muscles, potentially raising the energetic cost of walking. Several studies challenge this view, however, demonstrating no strong associations between pelvic width and increased energy expenditure, suggesting that musculoskeletal compensation minimizes locomotor costs via muscle coordination and posture dynamics. Because assessment of evolutionary constraints requires examination of forms outside observed species variation, we used musculoskeletal simulations to evaluate how extreme deviations in functional hip width affect gait parameters. Motion capture and ground reaction data from ten adults (45 trials) drove subject-specific musculoskeletal models where functional hip widths were altered by ±20% and ±40%. Simulations used identical gait kinematics to isolate the effect of pelvic breadth. Wider pelves increased hip abductor force and metabolic power during mid-stance, while narrower pelves showed inverse trends. Compensatory changes in antagonistic muscle groups (e.g. adductors and flexors) limited net effects. Across all pelvic conditions, the dominant contributors to stride and muscle metabolic cost remained consistent, and total muscle metabolic power stayed within observed inter-individual variation. These results support hypotheses suggesting human musculoskeletal systems can adapt to extreme shifts in pelvic width without substantial muscle metabolic penalties. Contrary to core assumptions of the OD, pelvic breadth alone does not constrain walking effectiveness, suggesting that pelvic evolution was shaped primarily by reproductive, developmental, and structural pressures than by locomotor energetics alone.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148307140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Three-dimensional analysis of spawning behaviour of skipjack tuna Katsuwonus pelamis using stereo-image measurements.","authors":"Hiroto Yamamoto, Akira Sasaki, Tomoki Kanna, Yasushi Mitsunaga, Shinsuke Torisawa","doi":"10.1242/bio.062671","DOIUrl":"10.1242/bio.062671","url":null,"abstract":"<p><p>Although many pelagic fish species exhibit group spawning, the mechanisms underlying this mating system remain unclear; more fundamentally, detailed descriptions of group-spawning behaviour itself are lacking. Here, the spawning behaviour of skipjack tuna, Katsuwonus pelamis, characterised by 'group spawning', was recorded in an aquarium tank using stereo-image measurements to estimate the size of reproductive participants and quantify swimming kinematics during reproduction. Events were observed in which an individual (referred to as the 'leader') was chased by multiple conspecifics (referred to as the 'follower') and subsequently engaged in spawning. However, leaders (presumed female) were rarely chased by multiple followers (presumed male), a scenario more conducive to visible sperm release. In contrast, single followers chased leaders of varying body sizes, including smaller and likely immature individuals. Furthermore, leaders chased by multiple followers darted and turned at higher speeds. Reproduction may begin with one male pursuing one female; when the male targets a female with a high likelihood of spawning, the female accelerates, potentially attracting additional males to join the chase. By linking locomotor performance to reproductive outcomes, this study advances understanding of group-spawning mechanisms in pelagic fish species and contributes to broader theories of sexual selection and mating system evolution.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13488411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology OpenPub Date : 2026-07-15Epub Date: 2026-07-14DOI: 10.1242/bio.062726
Nataliya Timoshevskaya, Raissa F Cecil, James Schwartz, Jeramiah J Smith, S Randal Voss
{"title":"Histone and non-histone protein acetylation in a tail regeneration model (Ambystoma mexicanum).","authors":"Nataliya Timoshevskaya, Raissa F Cecil, James Schwartz, Jeramiah J Smith, S Randal Voss","doi":"10.1242/bio.062726","DOIUrl":"10.1242/bio.062726","url":null,"abstract":"<p><p>Histone deacetylase inhibitors (HDACi) potently inhibit appendage regeneration, but how HDACi alter protein acetylation is relatively unexplored. Using genomic, transcriptomic, and proteomic approaches, we report HDACi-mediated changes in gene expression and protein acetylation at the outset of Ambystoma mexicanum tail regeneration. HDACi (romidepsin) treatment globally reversed a genome-wide deacetylation response after tail amputation, broadly increasing H3K9ac and H3K27ac within intergenic regions, gene promoters, and gene bodies. Changes in promoter acetylation were only weakly associated with changes in gene expression when considering all acetylated genes. However, the magnitude of acetylation change was significantly greater for upregulated genes, including previously identified regeneration-inhibitory genes. We further detected hundreds of differentially acetylated non-histone proteins after tail amputation, including proteins that function in transcriptional regulation and hemostasis. We used a small molecule (A-485) to inhibit differentially acetylated transcription factors (Crebbp/Ep300) to show their requirement for tail regeneration. A-485 downregulated many of the same genes that were downregulated by romidepsin, but the upregulated gene set was relatively unique. Our results implicated histone and non-histone protein acetylation in the early transcriptional regulation of regeneration associated genes.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148223204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology OpenPub Date : 2026-07-15DOI: 10.1242/bio.062593
Fabio Cortesi, Karen L Cheney, Georgina M Schlub, Zuzana Musilova, Terry J Ord
{"title":"Opsin gene evolution in aquatic, amphibious and terrestrial blennies (Blenniidae).","authors":"Fabio Cortesi, Karen L Cheney, Georgina M Schlub, Zuzana Musilova, Terry J Ord","doi":"10.1242/bio.062593","DOIUrl":"10.1242/bio.062593","url":null,"abstract":"<p><p>Evolutionary adaptations to life on land include changes to an animal's physiology, morphology and behaviour. The visual systems of amphibious fishes show pronounced morphological adaptations; however, whether molecular changes also occur remains largely unknown. Here, we investigated the molecular evolution of visual opsin genes in blennies (Blenniidae), with a primary focus on the amphibious and terrestrial Salariini. Using retinal transcriptomes and amino acid comparisons in nine species from fully aquatic to terrestrial, we found limited sequence changes and no correlation between habitat and cone opsin gene expression. The 'red-sensitive' lws, the 'green-sensitive' rh2a, and two 'blue-sensitive' sws2aα and sws2aβ paralogues were expressed in all species, with the latter two showing pronounced phylogenetic inertia. Long-wavelength-dominated vision is likely beneficial for feeding on algae and detritus, the primary food source of most study species, and may be co-adapted to perceive red-coloured displays in terrestrial blennies. Conversely, a lack of 'ultraviolet-sensitive' sws1 expression coincides with ultraviolet-absorbing lenses in blennies, which likely evolved to protect the retina from the damaging effects of short-wavelength radiation, independent of habitat. Our data suggest that, at the molecular level, the visual systems that evolved in aquatic blennies have been retained in species that have progressively transitioned onto land.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology OpenPub Date : 2026-07-15Epub Date: 2026-07-28DOI: 10.1242/bio.062757
Hunter J Weaver, Kimberly A Turner, Van Thuan Nguyen, Noah T Ashley
{"title":"Pharmacological corticosterone suppresses pro-inflammatory cytokine gene expression in a tissue-dependent manner independent of sleep fragmentation in male mice.","authors":"Hunter J Weaver, Kimberly A Turner, Van Thuan Nguyen, Noah T Ashley","doi":"10.1242/bio.062757","DOIUrl":"10.1242/bio.062757","url":null,"abstract":"<p><p>Disrupted sleep from obstructive sleep apnea or insomnia promotes an inflammatory environment. Studies in mice have shown that experimental sleep fragmentation (SF) increases pro-inflammatory cytokine gene expression in the brain and peripheral tissues as well as increases circulating glucocorticoids. Although glucocorticoids classically exert anti-inflammatory effects at pharmacological concentrations, they may also modulate immune function in a context- and dose-dependent manner. To investigate whether glucocorticoids regulate pro-inflammatory responses, we used a classical adrenalectomy (ADX) ablation and corticosterone (CORT) replacement design. Adult male C57BL/6J mice received bilateral ADX, sham surgery, or ADX with CORT replacement in drinking water and were exposed to acute (24 h) SF or no SF (NSF). Pro-inflammatory gene expression (interleukin-1 beta and tumor necrosis factor-alpha) was quantified in the hippocampus, hypothalamus, and prefrontal cortex, as well as peripheral tissues including the liver, spleen, heart, and epididymal white adipose tissue. ADX effectively reduced serum CORT, while CORT replacement produced pharmacological hormone levels. Pharmacological CORT treatment robustly suppressed pro-inflammatory gene expression across multiple brain regions (prefrontal cortex, hypothalamus) and peripheral tissues (liver, heart, spleen) compared with ADX and/or sham mice, indicating a strong anti-inflammatory effect at elevated glucocorticoid concentrations. In contrast, ADX mice showed modest, tissue-specific increases in inflammatory gene expression, primarily in the liver and heart, suggesting limited regulation by physiological CORT levels. SF did not significantly increase pro-inflammatory gene expression compared with controls. In conclusion, CORT exerts potent, dose-dependent, and tissue-specific suppression of pro-inflammatory gene expression that is not modulated by acute SF in male mice.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13474571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology OpenPub Date : 2026-07-15Epub Date: 2026-07-20DOI: 10.1242/bio.062699
Kai Koike, Matasaburo Fukutomi, Yuichi Takeuchi
{"title":"Prey capture strategies relate to prey position in the scale-eating cichlid Perissodus microlepis.","authors":"Kai Koike, Matasaburo Fukutomi, Yuichi Takeuchi","doi":"10.1242/bio.062699","DOIUrl":"10.1242/bio.062699","url":null,"abstract":"<p><p>Predators have evolved sophisticated behavioral patterns that enable efficient prey capture. The scale-eating cichlid Perissodus microlepis exhibits pronounced morphological asymmetry in mouth-opening direction, corresponding to behavioral laterality in attack direction. Given that recognizing and tracking the flank of prey are crucial for successful predation, we hypothesized that the morphological asymmetry also influences their spatial positioning relative to prey during attack initiation. To test this idea, we simultaneously tracked predator and prey movements using DeepLabCut and analyzed the predatory sequence. Not only strike direction but also the lateralized approach initiation position was correlated with mouth asymmetry. Additionally, trajectory analyses revealed two distinct predation strategies based on the predator's relative position to the prey at the start of the approach: a rear approach, whereby predators move in from behind and strike at close range with strong body flexion, and a side approach, whereby predators initiate from lateral positions at shorter distances and rush forward. Although rear and side attacks differ in their kinematics and strike timing, both strategies achieve high predation success by applying strong forces to the prey's flank. Overall, this study advances our understanding of the predation strategies of scale-eating cichlids, providing insights into their evolutionary significance.</p>","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"15 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}