Biology Open最新文献_第5页

Elemental stoichiometry and insect chill tolerance: evolved and plastic changes in organismal Na+ and K+ content in Drosophila. 元素化学计量学和昆虫抗寒性：果蝇有机Na+和K+含量的进化和塑性变化。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-30 DOI: 10.1242/bio.060597

Sarah C Chalmer, Seth M Rudman, Mads K Andersen, Paul Schmidt, Heath A MacMillan

{"title":"Elemental stoichiometry and insect chill tolerance: evolved and plastic changes in organismal Na+ and K+ content in Drosophila.","authors":"Sarah C Chalmer, Seth M Rudman, Mads K Andersen, Paul Schmidt, Heath A MacMillan","doi":"10.1242/bio.060597","DOIUrl":"10.1242/bio.060597","url":null,"abstract":"Acclimation and evolutionary adaptation can produce phenotypic changes that allow organisms to cope with challenges. Determining the relative contributions and the underlying mechanisms driving phenotypic shifts from acclimation and adaptation is of central importance to understanding animal responses to change. Rates of evolution have traditionally been considered slow relative to ecological processes that shape biodiversity. Many organisms nonetheless show patterns of genetic variation that suggest that adaptation may act sufficiently fast to allow continuous change in phenotypes in response to environmental change (called 'adaptive tracking'). In Drosophila, both plastic and evolved differences in chill tolerance are associated with ionoregulation. Here, we combine an acclimation experiment, field collections along a well-characterized latitudinal cline, and a replicated field experiment to assess the concordance in the direction, magnitude, and potential mechanisms of acclimation and adaptation on chill coma recovery and elemental (Na and K) stoichiometry in both sexes of Drosophila melanogaster. Acclimation strongly shaped chill coma recovery, spatial adaptation produced comparatively modest effects, and temporal adaptation had no significant effect. Leveraging knowledge on the mechanisms underlying variation in chill tolerance traits, we find that relationships between elemental stoichiometry and chill coma recovery in the context of acclimation may differ from those that are associated with spatial adaptive change.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral scanning and fluorescence lifetime imaging microscopy (FLIM) enable separation and characterization of C. elegans autofluorescence in the cuticle and gut. 光谱扫描和荧光寿命成像显微镜（FLIM）可以分离和表征秀丽隐杆线虫角质层和肠道中的自身荧光。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-30 DOI: 10.1242/bio.060613

Heino J Hulsey-Vincent, Elizabeth A Cameron, Caroline L Dahlberg, Domenico F Galati

{"title":"Spectral scanning and fluorescence lifetime imaging microscopy (FLIM) enable separation and characterization of C. elegans autofluorescence in the cuticle and gut.","authors":"Heino J Hulsey-Vincent, Elizabeth A Cameron, Caroline L Dahlberg, Domenico F Galati","doi":"10.1242/bio.060613","DOIUrl":"10.1242/bio.060613","url":null,"abstract":"Caenorhabditis elegans gut and cuticle produce a disruptive amount of autofluorescence during imaging. Although C. elegans autofluorescence has been characterized, it has not been characterized at high resolution using both spectral and fluorescence lifetime-based approaches. We performed high resolution spectral scans of whole, living animals to characterize autofluorescence of adult C. elegans. By scanning animals at 405 nm, 473 nm, 561 nm, and 647 nm excitations, we produced spectral profiles that confirm the brightest autofluorescence has a clear spectral overlap with the emission of green fluorescent protein (GFP). We then used fluorescence lifetime imaging microscopy (FLIM) to further characterize autofluorescence in the cuticle and the gut. Using FLIM, we were able to isolate and quantify dim GFP signal within the sensory cilia of a single pair of neurons that is often obscured by cuticle autofluorescence. In the gut, we found distinct spectral populations of autofluorescence that could be excited by 405 nm and 473 nm lasers. Further, we found lifetime differences between subregions of this autofluorescence when stimulated at 473 nm. Our results suggest that FLIM can be used to differentiate biochemically unique populations of gut autofluorescence without labeling. Further studies involving C. elegans may benefit from combining high resolution spectral and lifetime imaging to isolate fluorescent protein signal that is mixed with background autofluorescence and to perform useful characterization of subcellular structures in a label-free manner.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between locomotion and hindlimb morphology in the leopard (Panthera pardus) using a geometric morphometric approach. 用几何形态计量学方法研究豹（Panthera pardus）运动与后肢形态之间的关系。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-24 DOI: 10.1242/bio.061823

Riyanta Naidoo, Safiyyah Iqbal

{"title":"The relationship between locomotion and hindlimb morphology in the leopard (Panthera pardus) using a geometric morphometric approach.","authors":"Riyanta Naidoo, Safiyyah Iqbal","doi":"10.1242/bio.061823","DOIUrl":"10.1242/bio.061823","url":null,"abstract":"Felid bone morphology is highly influenced by factors such as locomotion, body size, and foraging behaviour. Understanding how these factors influence bone morphology is important for interpreting the behaviour and ecology of such species. This study aimed to determine the extent to which Panthera pardus (i.e. leopard) hindlimb morphology differs from that of other Panthera species, particularly Panthera leo (i.e. lion). Landmark-based geometric morphometric analyses were used to compare 27 Panthera femurs in the anterior and posterior views, by the use of principal component analyses. Distinct clusters were found linking the Panthera species for both the anterior and posterior views, inferring a difference in the femur morphology of the species. The Procrustes ANOVA regression further showed a significant difference in the mean shape between the Panthera femurs, for both the anterior and posterior views. A clear relationship was found between femur morphology and body size, with leopards possessing a more gracile and elongated femur to support a smaller body mass and lions possessing a more robust and stunted femur to support a larger body mass. It was found that femur morphology also correlates with locomotive flexibility and hunting success in felids. Leopard femur morphology aids in speed and flexibility during hunting, as well as aids in propulsion that allows for arboreal locomotion. It was ultimately deduced that femur morphology differs between Panthera species, according to their mechanical demands during locomotion.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 12","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response to Correspondence: Hajnal et al. 'The role of lin-12 notch in C. elegans anchor cell proliferation'. 回复：Hajnal等。“in-12 notch在线虫锚定细胞增殖中的作用”。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-30 DOI: 10.1242/bio.061817

Michael A Q Martinez, David Q Matus

引用次数: 0

Characterization of nitric oxide in Octopus maya nervous system and its potential role in sensory perception. 章鱼玛雅神经系统中一氧化氮的特征及其在感官知觉中的潜在作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-11-28 DOI: 10.1242/bio.061756

Fabián Vergara-Ovalle, Martha León-Olea, Eduardo Sánchez-Islas, Francisco Pellicer

引用次数: 0

Maternal immune activation does not affect maternal microchimeric cells. 母体免疫激活不影响母体嵌合细胞。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-23 DOI: 10.1242/bio.061830

Alexandria Borges, Naoki Irie

{"title":"Maternal immune activation does not affect maternal microchimeric cells.","authors":"Alexandria Borges, Naoki Irie","doi":"10.1242/bio.061830","DOIUrl":"10.1242/bio.061830","url":null,"abstract":"We are naturally chimeras. Apart from our own cells originating from the fertilized egg, placental mammals receive small numbers of maternal cells called maternal microchimerism (MMc) that persist throughout one's whole life. Not only are varying frequencies of MMc cells reported in seemingly contradicting phenomena, including immune tolerance and possible contribution to autoimmune-like disease, but frequencies are observable even among healthy littermates showing varying MMc frequencies and cell type repertoire. These varying differences in MMc frequencies or cell types could be contributing to the diverse phenomena related to MMc. However, factors biasing these MMc differences remain largely unknown. Here, we tested whether immunological activation leads to differing MMc frequencies, based on our recent study that suggests that most maternal cells are immune-related. Unexpectedly, fluorescence-activated cell sorting analysis on the murine spleen, thymus, and liver following maternal immune activation by mid-gestational lipopolysaccharide intraperitoneal injections detected no significant difference in the number, or ratio of, immune-related maternal cells in the tested embryonic organs of healthy offspring. These findings suggest that MMc frequencies remain stable even under immune-activated conditions, implying a possible control system of MMc migration against changes in the immunological conditions.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 12","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11695574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of lin-12 notch in C. elegans anchor cell proliferation. in-12缺口在线虫锚定细胞增殖中的作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-30 DOI: 10.1242/bio.061816

Alex Hajnal, Ting Deng, Evelyn Lattmann

{"title":"The role of lin-12 notch in C. elegans anchor cell proliferation.","authors":"Alex Hajnal, Ting Deng, Evelyn Lattmann","doi":"10.1242/bio.061816","DOIUrl":"10.1242/bio.061816","url":null,"abstract":"The gonadal anchor cell (AC) is an essential organizer for the development of the egg-laying organ in the C. elegans hermaphrodite. Recent work has investigated the mechanisms that control the quiescent state the AC adopts while fulfilling its functions. In this context, the transcription factors EGL-43 and NHR-67 are required to maintain the G1 cell cycle arrest of the AC and prevent proliferation. While NHR-67 acts primarily by up-regulating the CDK inhibitor CKI-1, the role of EGL-43 in this process has been subject to debate. Deng et al. (2020) reported that inhibition of the notch receptor lin-12 by RNAi partially suppressed the AC proliferation phenotype caused by egl-43 RNAi. By contrast, Martinez et al. (2022) found that down-regulation of LIN-12 NOTCH via the auxin-inducible degradation system did not reduce AC proliferation. To resolve this issue, we performed egl-43 RNAi in the background of a lin-12 null allele and observed a similar suppression of AC proliferation as reported previously by Deng et al. (2020). Hence, AC proliferation caused by the downregulation of egl-43 partially depends on LIN-12 NOTCH signaling.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 12","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructing a doxycycline-inducible system for an epithelial-to-mesenchymal transition model in MCF10A cells. 构建强力霉素诱导的MCF10A细胞上皮-间质转化模型。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-09 DOI: 10.1242/bio.061790

Yaxuan Sun, Xun Zhou, Xiaohui Hu

{"title":"Constructing a doxycycline-inducible system for an epithelial-to-mesenchymal transition model in MCF10A cells.","authors":"Yaxuan Sun, Xun Zhou, Xiaohui Hu","doi":"10.1242/bio.061790","DOIUrl":"10.1242/bio.061790","url":null,"abstract":"Epithelial to mesenchymal transition (EMT) has been shown to play an essential role in the early stages of cancer cell invasion and metastasis. Inducible EMT models can initiate EMT in a controlled manner, thereby providing the opportunity to determine whether a cancer-associated gene influences cancer metastasis by triggering EMT. Moreover, different inducible EMT models enable the investigation of specific mechanisms of EMT modulation by various genes, facilitating a more precise understanding of how these genes influence cancer metastasis through the induction of EMT. Unfortunately, current inducible EMT models still present unmet needs. Therefore, we aimed to establish an inducible EMT model in MCF10A cells, a spontaneously immortalized human fibrocystic mammary cell line, by manipulating the expression of mouse Twist1 (mTwist1). In this study, we first compared the EMT induction capacity between human TWIST1 (hTWIST1) and mTwist1, and selected mTwist1 for further investigation. By monitoring the changes in epithelial and mesenchymal markers at different induction time points, we examined the EMT process in both polyclonal and monoclonal MCF10A cells that express doxycycline (DOX)-inducible mTwist1. Furthermore, our results showed that doxycycline-induced mTwist1 expression triggered EMT at a similar rate to TGFβ1-induced EMT in MCF10A cells. Additionally, this process was reversible upon DOX withdrawal. Thus, we have established a robust inducible EMT model in MCF10A cells, which can be used to further study cancer metastasis-driving genes.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 12","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defensive tactics: lessons from Drosophila. 防御战术：来自果蝇的教训。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-24 DOI: 10.1242/bio.061609

Madhumala K Sadanandappa, Subhana Ahmad, Robinson Mohanraj, Mrunal Ratnaparkhi, Shivaprasad H Sathyanarayana

引用次数: 0

Progressive Palaeontology 2024 conference report. 进步古生物学2024年会议报告。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-12-15 Epub Date: 2024-12-06 DOI: 10.1242/bio.061822

Hady George, Thomas Farrell, James R G Rawson, Isaura Aguilar-Pedrayes, Benton Walters, Kirsten E Flett

引用次数: 0