Biology Open最新文献_第10页

Normotensive metabolic syndrome in Transient Receptor Potential Canonical Channel type 1 Trpc1-/- mice. 删除 1 型瞬时受体电位典型通道基因的小鼠出现正常血压代谢综合征。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-07-15 Epub Date: 2024-07-31 DOI: 10.1242/bio.060280

Richard Matthew Atkins, Meghan Pantalia, Christopher Skaggs, Alexander Ku Lau, Muhammad Bilal Mahmood, Muhammad Mubeen Anwar, Lindsay Barron, Bonnie Eby, Usman Khan, Leo Tsiokas, Kai Lau

{"title":"Normotensive metabolic syndrome in Transient Receptor Potential Canonical Channel type 1 Trpc1-/- mice.","authors":"Richard Matthew Atkins, Meghan Pantalia, Christopher Skaggs, Alexander Ku Lau, Muhammad Bilal Mahmood, Muhammad Mubeen Anwar, Lindsay Barron, Bonnie Eby, Usman Khan, Leo Tsiokas, Kai Lau","doi":"10.1242/bio.060280","DOIUrl":"10.1242/bio.060280","url":null,"abstract":"Metabolic syndrome has become a global epidemic, affecting all developed countries and communities with growing economies. Worldwide, increasing efforts have been directed at curbing this growing problem. Mice deleted of the gene encoding Type 1 Transient Receptor Potential Canonical Channel (Trpc1) were found to weigh heavier than controls. They had fasting hyperglycemia and impaired glucose tolerance compared with wild-type controls. Beyond 1 year of age, plasma triglyceride level in Trpc1-/- mice was elevated. Plasma cholesterol levels tended to be higher than in controls. The livers of Trpc1-/- mice were heavier, richer in triglyceride, and more echogenic than those of controls on ultrasound evaluation. Hematocrit was lower in Trpc1-/- mice of both genders beginning at the second to third months of age in the absence of bleeding or hemolysis. Measured by the indirect tail-cuff method or by the direct arterial cannulation, blood pressures in null mice were lower than controls. We conclude that TRPC1 gene regulates body metabolism and that except for hypertension, phenotypes of mice after deletion of the Trpc1 gene resemble mice with metabolic syndrome, suggesting that this could be a good experimental model for future investigation of the pathogenesis and management of this disorder.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11317093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Older spiny mice (Acomys cahirinus) have delayed and spatially heterogenous ear wound regeneration. 老龄刺鼠（Acomys cahirinus）的耳部伤口再生延迟且在空间上存在异质性。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-07-15 Epub Date: 2024-10-10 DOI: 10.1242/bio.060565

Justin A Varholick, Jazmine Thermolice, Gizelle Godinez, Vanessa Dos Santos, Rishi Kondapaneni, Malcolm Maden

{"title":"Older spiny mice (Acomys cahirinus) have delayed and spatially heterogenous ear wound regeneration.","authors":"Justin A Varholick, Jazmine Thermolice, Gizelle Godinez, Vanessa Dos Santos, Rishi Kondapaneni, Malcolm Maden","doi":"10.1242/bio.060565","DOIUrl":"10.1242/bio.060565","url":null,"abstract":"The African spiny mouse (Acomys cahirinus) is a unique mammalian model of tissue regeneration, regenerating 4 mm ear-hole punches with cartilage, adipocytes, hair follicles, and muscle. However, the time to regenerate ear tissue varies from 20 to 90 days and muscle regeneration is inconsistent. Some report that older spiny mice have delayed regeneration without investigation on the regenerative capacity of muscle. We thought that delayed regeneration and inconsistent muscle regeneration could be linked via age-related nerve degeneration. While the current study found that spiny mice aged 6-9 months had delayed regeneration compared to 3-4 month-old spiny mice, the capacity of muscle regeneration was unrelated to age, and there was little evidence for age-related nerve degeneration. Instead, the regeneration of muscle, cartilage and adipocytes was spatially heterogeneous, declining in amount from the proximal to distal region of the regenerated tissue. Also, cartilage regeneration in the distal region was decreased in ≥22-month-old Acomys and adipocyte regeneration was decreased in those older than 6 months, compared to 3-4 month olds. While the underlying mechanisms for delayed and spatially heterogenous regeneration remain unclear, age and the spatial region of the regenerated tissue should be considered in experimental designs with spiny mice.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct roles of COMPASS subunits to Drosophila heart development. COMPASS 亚基对果蝇心脏发育的不同作用

IF 1.8 4区生物学

Biology Open Pub Date : 2024-07-15 Epub Date: 2024-10-17 DOI: 10.1242/bio.061736

Jun-Yi Zhu, Joyce van de Leemput, Zhe Han

{"title":"Distinct roles of COMPASS subunits to Drosophila heart development.","authors":"Jun-Yi Zhu, Joyce van de Leemput, Zhe Han","doi":"10.1242/bio.061736","DOIUrl":"10.1242/bio.061736","url":null,"abstract":"The multiprotein complexes known as the complex of proteins associated with Set1 (COMPASS) play a crucial role in the methylation of histone 3 lysine 4 (H3K4). In Drosophila, the COMPASS series complexes comprise core subunits Set1, Trx, and Trr, which share several common subunits such as ash2, Dpy30-L1, Rbbp5, and wds, alongside their unique subunits: Wdr82 for Set1/COMPASS, Mnn1 for Trx/COMPASS-like, and Ptip for Trr/COMPASS-like. Our research has shown that flies deficient in any of these common or unique subunits exhibited high lethality at eclosion (the emergence of adult flies from their pupal cases) and significantly shortened lifespans of the few adults that do emerge. Silencing these common or unique subunits led to severe heart morphological and functional defects. Moreover, specifically silencing the unique subunits of the COMPASS series complexes, Wdr82, Mnn1, and Ptip, in the heart results in decreased levels of H3K4 monomethylation and dimethylation, consistent with effects observed from silencing the core subunits Set1, Trx, and Trr. These findings underscore the critical roles of each subunit of the COMPASS series complexes in regulating histone methylation during heart development and provide valuable insights into their potential involvement in congenital heart diseases, thereby informing ongoing research in heart disease.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-assessment of the subcellular localization of Bazooka/Par-3 in Drosophila: no evidence for localization to the nucleus and the neuromuscular junction. 重新评估果蝇中 Bazooka/Par-3 的亚细胞定位：没有证据表明其定位在细胞核和神经肌肉接头处。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-06-15 Epub Date: 2024-06-27 DOI: 10.1242/bio.060544

Soya Kim, Jaffer Shahab, Elisabeth Vogelsang, Andreas Wodarz

{"title":"Re-assessment of the subcellular localization of Bazooka/Par-3 in Drosophila: no evidence for localization to the nucleus and the neuromuscular junction.","authors":"Soya Kim, Jaffer Shahab, Elisabeth Vogelsang, Andreas Wodarz","doi":"10.1242/bio.060544","DOIUrl":"10.1242/bio.060544","url":null,"abstract":"Bazooka/Par-3 (Baz) is an evolutionarily conserved scaffold protein that functions as a master regulator for the establishment and maintenance of cell polarity in many different cell types. In the vast majority of published research papers Baz has been reported to localize at the cell cortex and at intercellular junctions. However, there have also been several reports showing localization and function of Baz at additional subcellular sites, in particular the nuclear envelope and the neuromuscular junction. In this study we have re-assessed the localization of Baz to these subcellular sites in a systematic manner. We used antibodies raised in different host animals against different epitopes of Baz for confocal imaging of Drosophila tissues. We tested the specificity of these antisera by mosaic analysis with null mutant baz alleles and tissue-specific RNAi against baz. In addition, we used a GFP-tagged gene trap line for Baz and a bacterial artificial chromosome (BAC) expressing GFP-tagged Baz under control of its endogenous promoter in a baz mutant background to compare the subcellular localization of the GFP-Baz fusion proteins to the staining with anti-Baz antisera. Together, these experiments did not provide evidence for specific localization of Baz to the nucleus or the neuromuscular junction.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11225583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

microRNA-guided immunity against respiratory virus infection in human and mouse lung cells. 人和小鼠肺细胞在 microRNA 引导下对呼吸道病毒感染的免疫力。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-06-15 Epub Date: 2024-06-14 DOI: 10.1242/bio.060172

Ayaka Shibamoto, Yoshiaki Kitsu, Keiko Shibata, Yuka Kaneko, Harune Moriizumi, Tomoko Takahashi

{"title":"microRNA-guided immunity against respiratory virus infection in human and mouse lung cells.","authors":"Ayaka Shibamoto, Yoshiaki Kitsu, Keiko Shibata, Yuka Kaneko, Harune Moriizumi, Tomoko Takahashi","doi":"10.1242/bio.060172","DOIUrl":"10.1242/bio.060172","url":null,"abstract":"Viral infectivity depends on multiple factors. Recent studies showed that the interaction between viral RNAs and endogenous microRNAs (miRNAs) regulates viral infectivity; viral RNAs function as a sponge of endogenous miRNAs and result in upregulation of its original target genes, while endogenous miRNAs target viral RNAs directly and result in repression of viral gene expression. In this study, we analyzed the possible interaction between parainfluenza virus RNA and endogenous miRNAs in human and mouse lungs. We showed that the parainfluenza virus can form base pairs with human miRNAs abundantly than mouse miRNAs. Furthermore, we analyzed that the sponge effect of endogenous miRNAs on viral RNAs may induce the upregulation of transcription regulatory factors. Then, we performed RNA-sequence analysis and observed the upregulation of transcription regulatory factors in the early stages of parainfluenza virus infection. Our studies showed how the differential expression of endogenous miRNAs in lungs could contribute to respiratory virus infection and species- or tissue-specific mechanisms and common mechanisms could be conserved in humans and mice and regulated by miRNAs during viral infection.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11212637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural crest development and disorders: from patient to model system and back again - the NEUcrest conference. 神经嵴发育与疾病：从病人到模型系统再到病人--神经嵴会议。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-06-15 Epub Date: 2024-06-13 DOI: 10.1242/bio.060530

Marco Antonaci, Amy Kerr, Merin Lawrence, Francesca Lorenzini, Nitin Narwade, Chloé Paka, Anna Magdalena Wulf

{"title":"Neural crest development and disorders: from patient to model system and back again - the NEUcrest conference.","authors":"Marco Antonaci, Amy Kerr, Merin Lawrence, Francesca Lorenzini, Nitin Narwade, Chloé Paka, Anna Magdalena Wulf","doi":"10.1242/bio.060530","DOIUrl":"10.1242/bio.060530","url":null,"abstract":"The neural crest (NC) is an embryonic multipotent and transitory population of cells that appears during late gastrulation/early neurulation in the developing embryos of vertebrate organisms. Often called \"the fourth germ layer\", the NC is characterised by incredible mobility, which allows the NC cells to migrate throughout the whole embryo, giving rise to an astonishing number of different derivatives in the adult organism, such as craniofacial skeleton, adrenal gland, enteric nervous system and melanocytes. Because of these properties, neurocristopathies (NCPs), which is the term used to classify genetic diseases associated with NC developmental defects, are often syndromic and, taken all together, are the most common type of genetic disease. The NEUcrest consortium is an EU funded innovative training network (ITN) that aims to study the NC and NCPs. In March 2024, the early stage researchers (ESRs) in the NEUcrest consortium organised an in-person conference for well-established and early career researchers to discuss new advances in the NC and NCPs field, starting from the induction of the NC, and then moving on to migration and differentiation processes they undergo. The conference focused heavily on NCPs associated with each of these steps. The conference also included events, such as a round table to discuss the future of the NC research, plus a talk by a person living with an NCP. This 3-day conference aimed to bring together the past, present and future of this field to try and unravel the mysteries of this unique cell population.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11190565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing metabolic rates in zebrafish using a 3D-printed intermittent-flow respirometer and swim tunnel system. 利用三维打印间歇流呼吸器和游泳隧道系统评估斑马鱼的新陈代谢率。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-06-15 Epub Date: 2024-06-18 DOI: 10.1242/bio.060375

Rasmus Hejlesen, Freja Burkarl Scheffler, Clara Garfiel Byrge, Kasper Kjær-Sørensen, Claus Oxvig, Angela Fago, Hans Malte

引用次数: 0

The Endosomal Sorting Complex, ESCRT, has diverse roles in blood progenitor maintenance, lineage choice and immune response. 内体分拣复合物（ESCRT）在血液祖细胞维持、血系选择和免疫反应中发挥着多种作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-06-15 Epub Date: 2024-06-18 DOI: 10.1242/bio.060412

Arindam Ray, Yashashwinee Rai, Maneesha S Inamdar

{"title":"The Endosomal Sorting Complex, ESCRT, has diverse roles in blood progenitor maintenance, lineage choice and immune response.","authors":"Arindam Ray, Yashashwinee Rai, Maneesha S Inamdar","doi":"10.1242/bio.060412","DOIUrl":"10.1242/bio.060412","url":null,"abstract":"Most hematological malignancies are associated with reduced expression of one or more components of the Endosomal Sorting Complex Required for Transport (ESCRT). However, the roles of ESCRT in stem cell and progenitor maintenance are not resolved. Parsing signaling pathways in relation to the canonical role of ESCRT poses a challenge. The Drosophila hematopoietic organ, the larval lymph gland, provides a path to dissect the roles of cellular trafficking pathways such as ESCRT in blood development and maintenance. Drosophila has 13 core ESCRT components. Knockdown of individual ESCRTs showed that only Vps28 and Vp36 were required in all lymph gland progenitors. Using the well-conserved ESCRT-II complex as an example of the range of phenotypes seen upon ESCRT depletion, we show that ESCRTs have cell-autonomous as well as non-autonomous roles in progenitor maintenance and differentiation. ESCRT depletion also sensitized posterior lobe progenitors to respond to immunogenic wasp infestation. We also identify key heterotypic roles for ESCRT in position-dependent control of Notch activation to suppress crystal cell differentiation. Our study shows that the cargo sorting machinery determines the identity of progenitors and their adaptability to the dynamic microenvironment. These mechanisms for control of cell fate may tailor developmental diversity in multiple contexts.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11212638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single target acuity for moving targets in the common sunfish (Lepomis gibbosus). 普通太阳鱼（Lepomis gibbosus）对移动目标的单目标敏锐度。

IF 2.4 4区生物学

Biology Open Pub Date : 2024-06-15 Epub Date: 2024-06-06 DOI: 10.1242/bio.060455

Marius Hoppe, Caroline Spratte, Frederike D Hanke, Kenneth Sørensen

引用次数: 0

An arch worth revisiting: a study on the feline humeral supracondylar foramen and its evolutionary significance. 值得重新审视的拱门：关于猫科动物肱骨髁上孔及其进化意义的研究。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-06-15 Epub Date: 2024-06-25 DOI: 10.1242/bio.060420

Eimear Byrne, Robert D Johnston, David Kilroy, Sourav Bhattacharjee

{"title":"An arch worth revisiting: a study on the feline humeral supracondylar foramen and its evolutionary significance.","authors":"Eimear Byrne, Robert D Johnston, David Kilroy, Sourav Bhattacharjee","doi":"10.1242/bio.060420","DOIUrl":"10.1242/bio.060420","url":null,"abstract":"The supracondylar foramen with a (seemingly) osseous peripheral arch noticed on the medio-distal feline humeri had remained disputed among anatomists. Some scholars have argued in favor of homology between this foramen and the supracondyloid foramen formed in presence of the ligament of Struthers in humans. Other theories include its presence as a retinaculum holding the median nerve and brachial artery to their anatomical position in a flexed elbow. Unfortunately, these theories lack investigative rigor. The emergence of non-invasive imaging modalities, such as micro-computed tomography, has enabled researchers to inspect the internal anatomy of bones without dismantling them. Thus, a micro-computed tomographic investigation was conducted on three feline (Felis catus) humeri specimens while the internal anatomy of the supracondylar foramina was examined. Unlike the humerus, the thin peripheral arch of the feline supracondylar foramen failed to elicit any osseous trabeculae or foci of calcification. While adhering to the humeral periosteum at its origin, the non-osseous arch, typical of a muscular tendon, attaches into the bony saddle related to the medial humeral epicondyle suggestive of a tendon or aponeurotic extension of a (vestigial) brachial muscle, with the coracobrachialis longus emerging to be the most likely candidate.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11225584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0