Biology Open最新文献_第9页

An atlas of Brachypodium distachyon lateral root development. Brachypodium distachyon侧根发育图集。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-02 DOI: 10.1242/bio.060531

Cristovāo de Jesus Vieira Teixeira, Kevin Bellande, Alja van der Schuren, Devin O'Connor, Christian S Hardtke, Joop E M Vermeer

{"title":"An atlas of Brachypodium distachyon lateral root development.","authors":"Cristovāo de Jesus Vieira Teixeira, Kevin Bellande, Alja van der Schuren, Devin O'Connor, Christian S Hardtke, Joop E M Vermeer","doi":"10.1242/bio.060531","DOIUrl":"10.1242/bio.060531","url":null,"abstract":"The root system of plants is a vital part for successful development and adaptation to different soil types and environments. A major determinant of the shape of a plant root system is the formation of lateral roots, allowing for expansion of the root system. Arabidopsis thaliana, with its simple root anatomy, has been extensively studied to reveal the genetic program underlying root branching. However, to get a more general understanding of lateral root development, comparative studies in species with a more complex root anatomy are required. Here, by combining optimized clearing methods and histology, we describe an atlas of lateral root development in Brachypodium distachyon, a wild, temperate grass species. We show that lateral roots initiate from enlarged phloem pole pericycle cells and that the overlying endodermis reactivates its cell cycle and eventually forms the root cap. In addition, auxin signaling reported by the DR5 reporter was not detected in the phloem pole pericycle cells or young primordia. In contrast, auxin signaling was activated in the overlying cortical cell layers, including the exodermis. Thus, Brachypodium is a valuable model to investigate how signaling pathways and cellular responses have been repurposed to facilitate lateral root organogenesis.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of potential TAK1/Map3k7 phosphorylation targets in hypertrophy and cachexia models of skeletal muscle. 骨骼肌肥大和恶病质模型中潜在的 TAK1/Map3k7 磷酸化靶标分析

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-25 DOI: 10.1242/bio.060487

Fatemeh Nasehi, Cameron Rylance, Erin Schnell, Maslyn Ann Greene, Caroline Conway, Zachary Hough, Susan Duckett, Robin C Muise-Helmericks, Ann Catherine Foley

{"title":"Analysis of potential TAK1/Map3k7 phosphorylation targets in hypertrophy and cachexia models of skeletal muscle.","authors":"Fatemeh Nasehi, Cameron Rylance, Erin Schnell, Maslyn Ann Greene, Caroline Conway, Zachary Hough, Susan Duckett, Robin C Muise-Helmericks, Ann Catherine Foley","doi":"10.1242/bio.060487","DOIUrl":"10.1242/bio.060487","url":null,"abstract":"TGFβ-activated kinase-1 (TAK1) is phosphorylated during both muscle growth and muscle wasting. To understand how this can lead to such opposite effects, we first performed multiplex kinase array of mouse embryonic stem cells with and without stimulation of TAK1 to determine its potential downstream targets. The phosphorylation of these targets was then compared in three different models: hypertrophic longissimus muscle of Texel sheep, tibialis anterior muscle of mice with cancer-induced cachexia and C2C12-derived myofibers, with and without blockade of TAK1 phosphorylation. In both Texel sheep and in cancer-induced cachexia, phosphorylation of both TAK1 and p38 was increased. Whereas p90RSK was increased in Texel sheep but not cachexia and the phosphorylation of HSP27 and total Jnk were increased in cachexia but not Texel. To understand this further, we examined the expression of these proteins in C2C12 cells as they differentiated into myotubes, with and without blockade of TAK1 phosphorylation. In C2C12 cells, decreased phosphorylation of TAK1 leads to reduced phosphorylation of p38, JNK, and HSP27 after 16 h and muscle fiber hypertrophy after 3 days. However, continuous blockade of this pathway leads to muscle fiber failure, suggesting that the timing of TAK1 activation controls the expression of context-dependent targets.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Year at the Forefront of Streptophyte Algal Evolution. 链藻进化前沿的一年。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-19 DOI: 10.1242/bio.061673

Alexander M C Bowles

引用次数: 0

A novel technique for atraumatic transurethral catheterisation of male rats. 雄性大鼠无创伤经尿道导尿的新技术。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-08-30 DOI: 10.1242/bio.060476

Steven Liben Zhang, Allen Wei-Jiat Wong

{"title":"A novel technique for atraumatic transurethral catheterisation of male rats.","authors":"Steven Liben Zhang, Allen Wei-Jiat Wong","doi":"10.1242/bio.060476","DOIUrl":"10.1242/bio.060476","url":null,"abstract":"Transurethral catheterisation of male rats is technically difficult owing to anatomical peculiarities. In the male rat, the urethral striated sphincter consists of two lateral fascicles separated by an anterior and a posterior strip of connective tissue, which impedes the smooth insertion of a urinary catheter. For rat studies requiring continuous collection of urine, bladder irrigation, or measurement of bladder pressure, investigators either have to exclude the male population (be limited to the female population) or perform percutaneous (suprapubic) bladder puncture in male rats, which is more traumatic and invasive than transurethral catheterisation. This paper describes a novel, atraumatic method of transurethral catheterisation in the male rat, with the aid of a microscope and microsurgical instruments. Six Wistar rats were used for this experiment, all of which were catheterised successfully, with no evidence of bladder or urethral injury. The study shows that male rats can be safely catheterised via the urethra with the aid of a microscope and microsurgical instruments for both visual and tactile feedback. This is a relatively straightforward technique to learn and can allow for inclusion of male rats in future studies requiring urinary analysis or bladder irrigation, without the need for traumatic percutaneous (suprapubic) bladder puncture.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11381925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of BMP signaling and stable gene expression in the early Drosophila embryo. 果蝇早期胚胎中 BMP 信号传递和稳定基因表达的动态变化

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-08-29 DOI: 10.1242/bio.061646

Hadel Al Asafen, Aydin Beseli, Hung-Yuan Chen, Sharva Hiremath, Cranos M Williams, Gregory T Reeves

{"title":"Dynamics of BMP signaling and stable gene expression in the early Drosophila embryo.","authors":"Hadel Al Asafen, Aydin Beseli, Hung-Yuan Chen, Sharva Hiremath, Cranos M Williams, Gregory T Reeves","doi":"10.1242/bio.061646","DOIUrl":"10.1242/bio.061646","url":null,"abstract":"In developing tissues, morphogen gradients are thought to initialize gene expression patterns. However, the relationship between the dynamics of morphogen-encoded signals and gene expression decisions is largely unknown. Here we examine the dynamics of the Bone Morphogenetic Protein (BMP) pathway in Drosophila blastoderm-stage embryos. In this tissue, the BMP pathway is highly dynamic: it begins as a broad and weak signal on the dorsal half of the embryo, then 20-30 min later refines into a narrow, intense peak centered on the dorsal midline. This dynamical progression of the BMP signal raises questions of how it stably activates target genes. Therefore, we performed live imaging of the BMP signal and found that dorsal-lateral cells experience only a short transient in BMP signaling, after which the signal is lost completely. Moreover, we measured the transcriptional response of the BMP target gene pannier in live embryos and found it to remain activated in dorsal-lateral cells, even after the BMP signal is lost. Our findings may suggest that the BMP pathway activates a memory, or 'ratchet' mechanism that may sustain gene expression.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11381920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase. 通过 CRISPR-Cas9 和 phiC31 整合酶实现瞬时可视基因分型和便捷的特定位点转基因。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-03 DOI: 10.1242/bio.061666

Junyan Ma, Weiting Zhang, Simin Rahimialiabadi, Nikkitha Umesh Ganesh, Zhengwang Sun, Saba Parvez, Randall T Peterson, Jing-Ruey Joanna Yeh

{"title":"Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase.","authors":"Junyan Ma, Weiting Zhang, Simin Rahimialiabadi, Nikkitha Umesh Ganesh, Zhengwang Sun, Saba Parvez, Randall T Peterson, Jing-Ruey Joanna Yeh","doi":"10.1242/bio.061666","DOIUrl":"10.1242/bio.061666","url":null,"abstract":"Here, we introduce 'TICIT', targeted integration by CRISPR-Cas9 and integrase technologies, which utilizes the site-specific DNA recombinase - phiC31 integrase - to insert large DNA fragments into CRISPR-Cas9 target loci. This technique, which relies on first knocking in a 39-basepair phiC31 landing site via CRISPR-Cas9, enables researchers to repeatedly perform site-specific transgenesis at the exact genomic location with high precision and efficiency. We applied this approach to devise a method for the instantaneous determination of a zebrafish's genotype simply by examining its color. When a zebrafish mutant line must be propagated as heterozygotes due to homozygous lethality, employing this method allows facile identification of a population of homozygous mutant embryos even before the mutant phenotypes manifest. Thus, it should facilitate various downstream applications, such as large-scale chemical screens. We demonstrated that TICIT could also create reporter fish driven by an endogenous promoter. Further, we identified a landing site in the tyrosinase gene that could support transgene expression in a broad spectrum of tissue and cell types. In sum, TICIT enables site-specific DNA integration without requiring complex donor DNA construction. It can yield consistent transgene expression, facilitate diverse applications in zebrafish, and may be applicable to cells in culture and other model organisms.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutational cooperativity of RUNX1::RUNX1T1 isoform 9a and oncogenic NRAS in zebrafish myeloid leukaemia. 斑马鱼髓性白血病中 RUNX1::RUNX1T1 异构体 9a 和致癌 NRAS 的突变协同作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-08-30 DOI: 10.1242/bio.060523

Robyn Lints, Christina A Walker, Omid Delfi, Matthew Prouse, Mandy PohLui De Silva, Stefan K Bohlander, Andrew C Wood

{"title":"Mutational cooperativity of RUNX1::RUNX1T1 isoform 9a and oncogenic NRAS in zebrafish myeloid leukaemia.","authors":"Robyn Lints, Christina A Walker, Omid Delfi, Matthew Prouse, Mandy PohLui De Silva, Stefan K Bohlander, Andrew C Wood","doi":"10.1242/bio.060523","DOIUrl":"10.1242/bio.060523","url":null,"abstract":"RUNX1::RUNX1T1 (R::RT1) acute myeloid leukaemia (AML) remains a clinical challenge, and further research is required to model and understand leukaemogenesis. Previous zebrafish R::RT1 models were hampered by embryonic lethality and low penetrance of the malignant phenotype. Here, we overcome this by developing an adult zebrafish model in which the human R::RT1 isoform 9a is co-expressed with the frequently co-occurring oncogenic NRASG12D mutation in haematopoietic stem and progenitor cells (HSPCs), using the Runx1+23 enhancer. Approximately 50% of F0 9a+NRASG12D transgenic zebrafish developed signs of haematological disease between 5 and 14 months, with 27% exhibiting AML-like pathology: myeloid precursor expansion, erythrocyte reduction, kidney marrow hypercellularity and the presence of blasts. Moreover, only 9a+NRASG12D transplant recipients developed leukaemia with high rates of mortality within 40 days, inferring the presence of leukaemia stem cells. These leukaemic features were rare or not observed in animals expressing either the NRAS or 9a oncogenes alone, suggesting 9a and NRAS cooperation drives leukaemogenesis. This novel adult AML zebrafish model provides a powerful new tool for investigating the basis of R::RT1 - NRAS cooperativity with the potential to uncover new therapeutic targets.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11381922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data transformation and model selection in bivariate allometry. 二元异构测量中的数据转换和模型选择。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-16 DOI: 10.1242/bio.060587

Gary C Packard

{"title":"Data transformation and model selection in bivariate allometry.","authors":"Gary C Packard","doi":"10.1242/bio.060587","DOIUrl":"10.1242/bio.060587","url":null,"abstract":"Students of biological allometry have used the logarithmic transformation for over a century to linearize bivariate distributions that are curvilinear on the arithmetic scale. When the distribution is linear, the equation for a straight line fitted to the distribution can be back-transformed to form a two-parameter power function for describing the original observations. However, many of the data in contemporary studies of allometry fail to meet the requirement for log-linearity, thereby precluding the use of the aforementioned protocol. Even when data are linear in logarithmic form, the two-parameter power equation estimated by back-transformation may yield a misleading or erroneous perception of pattern in the original distribution. A better approach to bivariate allometry would be to forego transformation altogether and to fit multiple models to untransformed observations by nonlinear regression, thereby creating a pool of candidate models with different functional form and different assumptions regarding random error. The best model in the pool of candidate models could then be identified by a selection procedure based on maximum likelihood. Two examples are presented to illustrate the power and versatility of newer methods for studying allometric variation. It always is better to examine the original data when it is possible to do so.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"13 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High mobility group box 1 (HMGB1) is a potential disease biomarker in cell and mouse models of Duchenne muscular dystrophy. 高迁移率基团框 1（HMGB1）是杜氏肌营养不良症细胞和小鼠模型中一种潜在的疾病生物标志物。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-05 DOI: 10.1242/bio.060542

Rebecca A Slick, Jessica Sutton, Margaret Haberman, Benjamin S O'Brien, Jennifer A Tinklenberg, Aashay Mardikar, Mariah J Prom, Margaret Beatka, Melanie Gartz, Mark A Vanden Avond, Emily Siebers, David L Mack, J Patrick Gonzalez, Allison D Ebert, Kanneboyina Nagaraju, Michael W Lawlor

{"title":"High mobility group box 1 (HMGB1) is a potential disease biomarker in cell and mouse models of Duchenne muscular dystrophy.","authors":"Rebecca A Slick, Jessica Sutton, Margaret Haberman, Benjamin S O'Brien, Jennifer A Tinklenberg, Aashay Mardikar, Mariah J Prom, Margaret Beatka, Melanie Gartz, Mark A Vanden Avond, Emily Siebers, David L Mack, J Patrick Gonzalez, Allison D Ebert, Kanneboyina Nagaraju, Michael W Lawlor","doi":"10.1242/bio.060542","DOIUrl":"10.1242/bio.060542","url":null,"abstract":"Duchenne muscular dystrophy (DMD) is a progressive muscle wasting disorder affecting 1:3500 male births and is associated with myofiber degeneration, regeneration, and inflammation. Glucocorticoid treatments have been the standard of care due to immunomodulatory/immunosuppressive properties but novel genetic approaches, including exon skipping and gene replacement therapy, are currently being developed. The identification of additional biomarkers to assess DMD-related inflammatory responses and the potential efficacy of these therapeutic approaches are thus of critical importance. The current study uses RNA sequencing of skeletal muscle from two mdx mouse models to identify high mobility group box 1 (HMGB1) as a candidate biomarker potentially contributing to DMD-related inflammation. HMGB1 protein content was increased in a human iPSC-derived skeletal myocyte model of DMD and microdystrophin treatment decreased HMGB1 back to control levels. In vivo, HMGB1 protein levels were increased in vehicle treated B10-mdx skeletal muscle compared to B10-WT and significantly decreased in B10-mdx animals treated with adeno-associated virus (AAV)-microdystrophin. However, HMGB1 protein levels were not increased in D2-mdx skeletal muscle compared to D2-WT, demonstrating a strain-specific difference in DMD-related immunopathology.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Octopus maya enzyme activity of the digestive gland and gastric juice. 评估章鱼玛雅消化腺和胃液的酶活性。

IF 1.8 4区生物学

Biology Open Pub Date : 2024-09-15 Epub Date: 2024-09-12 DOI: 10.1242/bio.060429

Daisy Pineda-Suazo, Wendy Escobedo-Hinojosa, Lenin E Fabian-Canseco, Pedro Gallardo, Cintia Moguel-Ojeda, Claudia Caamal-Monsreal, Ariadna Sánchez-Arteaga, Carlos Rosas

{"title":"Evaluation of Octopus maya enzyme activity of the digestive gland and gastric juice.","authors":"Daisy Pineda-Suazo, Wendy Escobedo-Hinojosa, Lenin E Fabian-Canseco, Pedro Gallardo, Cintia Moguel-Ojeda, Claudia Caamal-Monsreal, Ariadna Sánchez-Arteaga, Carlos Rosas","doi":"10.1242/bio.060429","DOIUrl":"10.1242/bio.060429","url":null,"abstract":"As the demand for Octopus maya grows, sustainable farming practices become essential to prevent overexploitation, so that farming can be developed as a sustainable alternative to traditional fishing. Understanding the digestive dynamics of the octopus is essential for devising optimal dietary formulations in aquaculture. Despite the progress in understanding cephalopod digestion, little is known about the specific functioning of the digestive enzymes responsible for breaking down protein substrates. This knowledge gap underscores the need for further research to support sustainable O. maya population management. In this paper, dietary formulations are identified for cephalopods by characterizing O. maya digestive enzymes present in the digestive gland and gastric juice. The investigation revealed that acidic proteases showed a peak activity at higher temperatures than alkaline proteases. Inhibitors confirmed the presence of H, L, and D cathepsins. The lower activation energy of alkaline enzymes compared to acidic ones observed highlights an intriguing aspect of O. maya's digestive physiology. This research provides valuable insights into O. maya digestive enzyme functions, representing a significant advancement in formulating diets crucial for successful octopus farming that may help to fully understand its physiology.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0