Biology Open最新文献

First person - Julio Fierro Morales. 第一人称：胡里奥·菲耶罗·莫拉莱斯。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-15 Epub Date: 2025-10-08 DOI: 10.1242/bio.062275

引用次数: 0

Stress-induced elemental retention in an ectothermic vertebrate. 异温脊椎动物应力引起的元素滞留。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-15 Epub Date: 2025-10-02 DOI: 10.1242/bio.062237

Avik Banerjee, Maria Thaker

{"title":"Stress-induced elemental retention in an ectothermic vertebrate.","authors":"Avik Banerjee, Maria Thaker","doi":"10.1242/bio.062237","DOIUrl":"10.1242/bio.062237","url":null,"abstract":"Physiological stress responses are energy intensive. Animals can meet their stress-induced energetic demands by altering foraging or selectively retaining ingested nutrients, although the latter is poorly studied. We tested the effects of elevated stress on elemental retention in Psammophilus dorsalis. Adult lizards of both sexes were allotted to either a stressed group (daily constraint) or control group for 10 days. We measured baseline corticosterone, glucose, and triglyceride levels of lizards at the beginning and end of the experiment, as well as the total amounts of carbon and nitrogen retained based on the ingested and egested content during the treatment phase. Both control and stressed groups had higher corticosterone levels at the end of the experiment, with stressed group males showing the greatest increase. Glucose and triglyceride levels were variable. Contrary to expectation, lizards from both treatments retained similar amounts of carbon and nitrogen during the experiment phase. Our results do not show support for changes in elemental retention under stressful conditions, although the stress of captivity itself could have masked the potential effect on elemental retention. Our study highlights the need to test elemental retention as a potential strategy to meet stress-induced energetic demands when foraging opportunities are limited.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7618149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulated piggyBac and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells. 用于工程转基因纯合和杂合eHAP细胞的绝缘piggyBac和FRT载体。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-15 Epub Date: 2025-10-01 DOI: 10.1242/bio.061793

Annabel Y Minard, Stanley Winistorfer, Robert C Piper

引用次数: 0

Differential PaxillinB dynamics at Dictyostelium cell-substrate adhesions. PaxillinB在盘状骨细胞-底物粘附中的差异动力学。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-15 Epub Date: 2025-09-26 DOI: 10.1242/bio.062197

Julio C Fierro Morales, Minna Roh-Johnson

{"title":"Differential PaxillinB dynamics at Dictyostelium cell-substrate adhesions.","authors":"Julio C Fierro Morales, Minna Roh-Johnson","doi":"10.1242/bio.062197","DOIUrl":"10.1242/bio.062197","url":null,"abstract":"Adhesion-based migration is regulated by focal adhesions: multi-protein nanostructures linking the intracellular cytoskeleton to the extracellular substrate. Efficient adhesion-based migration has been shown to be regulated by focal adhesion dynamics such as lifetime, size and turnover, which in turn are influenced by the molecular composition of focal adhesions. We recently identified the formation of cell-substrate adhesion populations in Dictyostelium discoideum with differing molecular compositions, but it is unclear how these distinct compositions influence Dictyostelium adhesion dynamics and cell migration. Here, we further investigate the role of VinculinB - the Dictyostelium homologue of Vinculin - localization on Dictyostelium adhesion lifetime and protein turnover during cell migration. We show that co-localization of VinculinB to PaxillinB-positive cell-substrate adhesions increases adhesion lifetime without changing PaxillinB turnover. We further show that truncation of the PaxillinB N-terminus, which perturbs VinculinB co-localization to adhesions, surprisingly also increases adhesion lifetime and decreases PaxillinB turnover at adhesions. These findings suggest that similar to mammalian focal adhesions, molecular composition of Dictyostelium cell-substrate adhesion regulates their adhesion lifetimes and protein turnover, providing insight into how cell-substrate adhesions function during Dictyostelium cell migration.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the burrow: Body condition and sex influence exploratory behavior in desert kangaroo rats (Dipodomys deserti). 洞穴之外：身体状况和性别影响沙漠袋鼠大鼠（Dipodomys deserti）的探索行为。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-15 Epub Date: 2025-10-13 DOI: 10.1242/bio.062164

Katrina Moore, Anahita Sadrossadat, Zhuoyang Zhang, Charles Huang, Joey Huynh, Craig McGowan, Monica Daley

{"title":"Beyond the burrow: Body condition and sex influence exploratory behavior in desert kangaroo rats (Dipodomys deserti).","authors":"Katrina Moore, Anahita Sadrossadat, Zhuoyang Zhang, Charles Huang, Joey Huynh, Craig McGowan, Monica Daley","doi":"10.1242/bio.062164","DOIUrl":"10.1242/bio.062164","url":null,"abstract":"Behavioral variation within a population can be influenced by physical factors such as size, sex, and body condition. This variation may contribute to intraspecific niche breadth by enabling individuals to exploit different niches. To examine how anatomy shapes behavior, we conducted open field tests on desert kangaroo rats (Dipodomys deserti, n=16) and compared their activity to sex, morphology, and body condition. We constructed an arena within the species' natural habitat to simulate ecologically relevant conditions and recorded behavior over 15 min. We quantified speed and distance traveled, used principal component analysis to explore behavioral patterns, and used linear models to test for associations between behavior, locomotor traits, and anatomical variables. We found that individuals with lower body condition scores spent more time exploring, males were more exploratory than females, and individuals with longer feet - a proxy for skeletal size - traveled further. However, behavior and locomotor performance were not significantly correlated. Lastly, individuals moved faster and farther on full moon nights compared to new moon nights, indicating that moonlight influences movement strategy - potentially reflecting trade-offs between foraging and predation risk. These findings highlight species-specific drivers of behavioral variation and underscore the importance of understanding behavioral variability of desert mammals.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of zebrafish fear responses to eight different fish species using three-dimensional locomotion-tracking assays. 斑马鱼对八种不同鱼类的恐惧反应的3D运动跟踪分析。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-15 Epub Date: 2025-10-08 DOI: 10.1242/bio.062110

Kevin Adi Kurnia, Gilbert Audira, Michael Edbert Suryanto, Tzong-Rong Ger, Chung-Der Hsiao

{"title":"Comparative analysis of zebrafish fear responses to eight different fish species using three-dimensional locomotion-tracking assays.","authors":"Kevin Adi Kurnia, Gilbert Audira, Michael Edbert Suryanto, Tzong-Rong Ger, Chung-Der Hsiao","doi":"10.1242/bio.062110","DOIUrl":"10.1242/bio.062110","url":null,"abstract":"Zebrafish (Danio rerio) are widely used in neurobehavioral research due to their translational relevance in studying fear. Eight different fish species and variations were tested to induce fear responses in zebrafish, including one positive control (convict cichlid, Amatitlania nigrofasciata) and negative control (tiger barb, Puntigrus tetrazona) through a shared-environment test. The observation was done in three dimensions (3D) and two dimensions (2D) to assess the impact of dimensionality on the outcome. A single-camera system was used to capture two viewpoints by mirror reflection installed above the fish tank and reconstructed to 3D using F3LA software. Zebrafish showed a similar behavioral response towards Demason's cichlid (Pseudotrophus demasoni) and threadfin acara (Acarichthys heckelii) as they did to A. nigrofasciata, with some minor differences, and a lesser response to green Gymnocorymbus ternetzi, during the shared-environment tests. Meanwhile, presence of B. melanopterus caused zebrafish to have a higher tendency to freeze and display higher entropy, similar to an anxiety-like response. We found no correlation between behavioral response and the body size of the test fishes. However, a correlation was observed when we tested convict cichlids of different ages. Finally, zebrafish color preference was also observed through the use of G. ternetzi with different body colors as test fish, with the zebrafish preferring orange and red G. ternetzi and mostly avoiding green G. ternetzi. We found use of 3D observation superior to 2D observation because several important endpoints are obtainable only from certain viewpoints.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the genetic architecture of migratory timing in a songbird migrant, the great reed warbler, Acrocephalus arundinaceus. 阐明鸣禽大苇莺（Acrocephalus arundinaceus）迁徙时间的遗传结构。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-09 DOI: 10.1242/bio.062039

Emily R Fackler, Dmitry Kishkinev, Petr Procházka, Robert R Fitak

{"title":"Elucidating the genetic architecture of migratory timing in a songbird migrant, the great reed warbler, Acrocephalus arundinaceus.","authors":"Emily R Fackler, Dmitry Kishkinev, Petr Procházka, Robert R Fitak","doi":"10.1242/bio.062039","DOIUrl":"https://doi.org/10.1242/bio.062039","url":null,"abstract":"Great reed warblers (Acrocephalus arundinaceus) have become an important species for understanding long-distance avian migration, yet the genetic basis of their migratory timing remains unknown. While previous studies have identified candidate genes influencing migration timing in other species, their role in great reed warblers remains unexplored. Additionally, it is unclear whether the genetic basis of migratory timing differs between spring and autumn migrations. This study aims to uncover genetic factors influencing migration timing, providing insights into the evolutionary and ecological processes shaping long-distance migration. We conducted pooled whole-genome sequencing representing four great reed warbler migratory chronotypes: early spring, late spring, early autumn, and late autumn. By comparing FST and allele frequency differences, we determined that the spring migration had a larger genetic contribution than the autumn migration; however, the effect sizes were small (0.03 and 0.001, respectively). When comparing the early and late spring pools, we identified 93 candidate genes enriched for functions related to lipid hydrolysis that putatively influence great reed warbler migratory behavior. Our results provide insight into the genetic differentiation underlying migratory timing in great reed warblers which is crucial for predicting how they will adapt to shifting environmental conditions due to climate change and habitat loss.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafish model reveals developmental and hematopoietic functions of ADAMTS13. 斑马鱼模型揭示了ADAMTS13的发育和造血功能。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-06 DOI: 10.1242/bio.062265

Samuele Sartori, Ignacio Babiloni Chust, Marco Varinelli, Alessandro Mattè, Piera Trionfini, Susanna Tomasoni, Lucia Poggi

{"title":"Zebrafish model reveals developmental and hematopoietic functions of ADAMTS13.","authors":"Samuele Sartori, Ignacio Babiloni Chust, Marco Varinelli, Alessandro Mattè, Piera Trionfini, Susanna Tomasoni, Lucia Poggi","doi":"10.1242/bio.062265","DOIUrl":"https://doi.org/10.1242/bio.062265","url":null,"abstract":"ADAMTS13 is a metalloprotease that cleaves the von Willebrand factor and prevents pathological thrombosis. Severe genetic deficiency of ADAMTS13 causes congenital thrombotic thrombocytopenic purpura, a life-threatening thrombotic microangiopathy. Increasing evidence suggests that ADAMTS13 contributes to physiological processes beyond hemostasis, including vascular development and tissue homeostasis, but these functions remain poorly understood. To address this gap, we generated a transparent, multitransgenic adamts13i5 zebrafish model and began investigating the developmental and disease-related roles of ADAMTS13 in vivo. The adamts13i5 mutants recapitulated hallmark features of congenital thrombotic thrombocytopenic purpura, including erythrocyte fragmentation and schistocyte formation in adults. In larvae, ADAMTS13 loss unveiled a prothrombotic response to vascular injury, a phenotype masked in patients by thrombocytopenia. Mechanistically, ADAMTS13 deficiency impaired developmental vascular patterning, suppressed vegfa expression, and reduced macrophage number, accompanied by diminished inflammatory and pro-angiogenic signaling. ADAMTS13 loss disrupted hematopoietic homeostasis in adulthood, with myeloid expansion and lymphoid depletion in the kidney marrow. These findings establish ADAMTS13 as a multifaceted regulator of thrombosis, vascular development, inflammation, and hematopoietic lineage specification. The adamts13i5 Zebrafish provides a powerful vertebrate model for dissecting the mechanisms of thrombotic thrombocytopenic purpura pathogenesis and identifying therapeutic strategies extending beyond hemostasis.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic stress and muscle mechanics: Acute response of isolated soleus and EDL muscles to prolonged fasting in mice with distinct muscle phenotypes. 代谢应激和肌肉力学：在具有不同肌肉表型的小鼠中，分离的比目鱼和EDL肌肉对长时间禁食的急性反应。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-10-06 DOI: 10.1242/bio.062245

L Cesanelli, B Ylaite, M Brazaitis, N Eimantas, A Ratkevicius, D Satkunskiene, P Minderis

{"title":"Metabolic stress and muscle mechanics: Acute response of isolated soleus and EDL muscles to prolonged fasting in mice with distinct muscle phenotypes.","authors":"L Cesanelli, B Ylaite, M Brazaitis, N Eimantas, A Ratkevicius, D Satkunskiene, P Minderis","doi":"10.1242/bio.062245","DOIUrl":"https://doi.org/10.1242/bio.062245","url":null,"abstract":"Prolonged fasting impacts skeletal muscle by inducing atrophy, thereby limiting contractile capacity and altering tissue mechanical behavior. This study investigated the effects of 48 h of fasting (FAS) versus ad libitum food consumption (CON) on the mechanical properties of fast-twitch (extensor digitorum longus, EDL) and slow-twitch (soleus, SOL) muscles in three mouse strains with distinct muscle phenotypes: C57BL/6J (normal-sized), BEH+/+ (larger muscles), and BEH (myostatin-deficient with markedly larger muscles). Isolated SOL and EDL were subjected to 100 isometric-eccentric contraction cycles, and peak and specific force, rate of force development, fatigue, stiffness, and tangent modulus were assessed. Fasting significantly reduced muscle size and force production capacity (isometric and eccentric) across all strains (p<0.05). SOL muscles showed a greater decline in tetanic force (fatigue index: SOL 67% vs. EDL 33%, p<0.05), while BEH mice exhibited the steepest contractile impairment (p<0.05). Fasting also reduced stiffness and tangent modulus across all strains and muscle types (p<0.05). These findings demonstrate that fasting consistently impairs contractile and mechanical properties of skeletal muscle, with slow-twitch muscles and larger muscles phenotypes being particularly vulnerable. Muscle type and genetic background thus play key roles in determining the extent of functional decline under metabolic stress.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Do honey phytochemicals modulate forager aggression and the gut microbiome in the honey bee (Apis mellifera L.)? 蜂蜜植物化学物质是否会调节蜜蜂的觅食攻击和肠道微生物群？

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-30 DOI: 10.1242/bio.062233

Wade A Pike, Jaesylin Stephens, Mariah Donohue, Katsuri Rajandran, Erin D Treanore, Abdallah Sher, Emily Croteau, Clare C Rittschof

{"title":"Do honey phytochemicals modulate forager aggression and the gut microbiome in the honey bee (Apis mellifera L.)?","authors":"Wade A Pike, Jaesylin Stephens, Mariah Donohue, Katsuri Rajandran, Erin D Treanore, Abdallah Sher, Emily Croteau, Clare C Rittschof","doi":"10.1242/bio.062233","DOIUrl":"https://doi.org/10.1242/bio.062233","url":null,"abstract":"Plant phytochemicals found in nectar impact bee learning and memory and plant pollination success. Especially for generalist pollinators, dietary changes that alter phytochemical consumption could be common sources of behavioral variation. For honey bee (Apis mellifera L.) foragers, a major potential change in phytochemical consumption occurs when individuals switch from collecting nectar from flowers to collecting honey from neighboring colonies, a phenomenon known as honey robbing. In this study we investigated whether phytochemicals dominant in honey compared to nectar act as a short-term trigger of robbing behaviors in honey bee, which include increased aggression. We fed forager honey bees sucrose diets containing different phytochemicals found in nectar and honey and tested aggression using a lab-based assay. We found no evidence that phytochemicals altered forager behavior. We also compared the microbiome composition for foragers fed different phytochemicals and again found no effects. Our results suggest that neither direct effects of neuroactive phytochemicals, nor indirect effects through the structure or function of the gut microbiome, trigger honey robbing behaviors.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0