Biology Open最新文献_第2页

A proposed GABAergic pathway in the feeding neural network is associated with learning performance in conditioned taste aversion in Lymnaea. 进食神经网络中的gaba能通路与淋巴细胞条件性味觉厌恶的学习表现有关。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-10-15 Epub Date: 2026-10-16 DOI: 10.1242/bio.062762

Ayaka Itoh, Samui Chiba, Yoshimasa Komatsuzaki, Minoru Saito

{"title":"A proposed GABAergic pathway in the feeding neural network is associated with learning performance in conditioned taste aversion in Lymnaea.","authors":"Ayaka Itoh, Samui Chiba, Yoshimasa Komatsuzaki, Minoru Saito","doi":"10.1242/bio.062762","DOIUrl":"10.1242/bio.062762","url":null,"abstract":"Conditioned taste aversion (CTA) provides a useful model for investigating the neural mechanisms underlying long-term memory (LTM). In Lymnaea stagnalis, CTA can be induced by pairing sucrose, which initially elicits feeding responses, with KCl, which suppresses feeding responses. Following such conditioning, snails exhibit a long-lasting reduction in feeding responses to sucrose, reflecting the formation of CTA-LTM. In the present study, however, individual snails exhibited marked variability in CTA-LTM performance. We therefore analyzed behavioral differences among learner phenotypes and examined how pharmacological manipulation of GABAergic signaling during CTA training influences learning performance. Behavioral analyses revealed that learner phenotypes were associated with differences in feeding responses to sucrose before training. External application of the GABAA receptor antagonist bicuculline significantly increased the proportion of good learners. Based on the present findings, together with previous results, we propose a working model incorporating a putative GABAergic pathway within the feeding neural network, possibly involving the cerebral giant cell, and suggest that this pathway may be associated with individual differences in learning performance. Although the precise neural mechanisms remain to be determined, the present study provides a framework for understanding how GABAergic signaling contributes to CTA-LTM formation and individual variation in learning performance.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From genetics to migration: emerging insights into salmonid responses to environmental change from NoWPaS 2026. 从遗传学到迁徙：从NoWPaS 2026开始对鲑鱼对环境变化的反应的新见解。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-10-15 Epub Date: 2026-10-02 DOI: 10.1242/bio.062614

Peter Betts, Hallie E Arno, Eliot Boulaire, Samantha Crowley, Anthony Fontaine, Phoebe Kaiser-Wilks, Gabrielle Ladurée, Aglaé H Lambert, Seán Neville, Vebjørn K Opsanger, Joris Philip, Hayleigh Pogmore, Anna E Silverstein

{"title":"From genetics to migration: emerging insights into salmonid responses to environmental change from NoWPaS 2026.","authors":"Peter Betts, Hallie E Arno, Eliot Boulaire, Samantha Crowley, Anthony Fontaine, Phoebe Kaiser-Wilks, Gabrielle Ladurée, Aglaé H Lambert, Seán Neville, Vebjørn K Opsanger, Joris Philip, Hayleigh Pogmore, Anna E Silverstein","doi":"10.1242/bio.062614","DOIUrl":"10.1242/bio.062614","url":null,"abstract":"The International Workshop of PhDs and Post-doctoral Fellows on Anadromous Salmonids (NoWPaS) provides a platform for early-career researchers to present and discuss emerging research on salmonid biology. The 2026 meeting, held in Iceland, brought together contributions spanning salmonid ecology, physiology, evolutionary genetics, conservation, and movement ecology. Here, we summarise the main themes of the meeting, highlighting how salmonids continue to serve as powerful model systems. Across sessions, studies emphasised the importance of intraspecific diversity, phenotypic plasticity, and local adaptation in shaping population dynamics, alongside advances in telemetry, genomics, and analytical approaches. Contributions also addressed key conservation challenges, including climate change, habitat fragmentation, and shifting trophic dynamics, while presenting novel tools and strategies to support management. Together, these findings reinforce the need for integrative, interdisciplinary approaches to understand and conserve salmonid populations in a rapidly changing world. The conference also facilitated holistic and collaborative research, continuing the long-held aim of NoWPaS of developing a network of early-career researchers from diverse backgrounds.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"15 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13670355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A protocol for sequential isolation, purification, and collection of Luticola nematophagus eggs to obtain high-quality genomic DNA. 序贯分离、纯化和收集食线虫卵以获得高质量基因组DNA的方案。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-10-15 Epub Date: 2026-10-01 DOI: 10.1242/bio.062663

Shouhui Pan, Chongtao Guo, Hai Zhang, Hao Cen, Quan Zhang, Fei Dai, Congyong Li, Qing Yang, Zhouqiong Zhang, Wang Yin, Renju Deng

{"title":"A protocol for sequential isolation, purification, and collection of Luticola nematophagus eggs to obtain high-quality genomic DNA.","authors":"Shouhui Pan, Chongtao Guo, Hai Zhang, Hao Cen, Quan Zhang, Fei Dai, Congyong Li, Qing Yang, Zhouqiong Zhang, Wang Yin, Renju Deng","doi":"10.1242/bio.062663","DOIUrl":"10.1242/bio.062663","url":null,"abstract":"Conventional strategies for obtaining planarian tissue for genomic DNA extraction rely on diet-based rearing or amputation-induced regeneration. These approaches are inapplicable to soil-dwelling flatworms that predominantly reproduce by egg-laying and lack regenerative capacity, such as the recently discovered nematode predator Luticola nematophagus. Here, we developed a protocol for the sequential isolation, purification, and collection of L. nematophagus eggs, comprising three steps: (1) physical sieving through sintered meshes of graded pore sizes under recirculating water flow to exclude nontarget eggs; (2) chemical digestion with 8% (w/w, weight/weight) urea or 5% (w/w) hydroxylamine hydrochloride under continuous aeration for 18 h to eliminate contaminating microinvertebrates; and (3) manual sorting under a stereomicroscope. Genomic DNA extracted from purified eggs exhibited A260/A280 ratios of 1.93-2.00 and A260/A230 ratios exceeding 2.0, meeting the quality thresholds for whole-genome sequencing. Successful PCR amplification of 18S and 28S ribosomal DNA (rDNA) fragments confirmed the suitability of the DNA for downstream molecular analyses. Compared with untreated controls, urea treatment (8% urea and manual sorting) reduced the final hatching rate by 5.2% and delayed the half-hatching time by 5.1 days, whereas hydroxylamine hydrochloride treatment (5% hydroxylamine hydrochloride and manual sorting) reduced the final hatching rate by 11.6% and delayed the half-hatching time by 7.9 days. However, the majority of the eggs remained viable. The quality of the extracted DNA was further confirmed by successful whole-genome sequencing, which yielded a chromosome-level genome assembly, indicating its suitability for the most demanding downstream applications.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"15 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13670354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlamydomonas γ-tubulin mutations reveal a critical role for γ-TuRC in maintaining the stability of centriolar microtubules. 衣藻γ-微管蛋白突变揭示了γ-TuRC在维持中心微管稳定性中的关键作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-10-12 DOI: 10.1242/bio.062609

Yuki Nakazawa, Naoto Kubota, Mao Horii, Akira Noga, Yoshikazu Koike, Hiroko Kawai-Toyooka, Hideo Dohra, Katsushi Yamaguchi, Shuji Shigenobu, Ken-Ichi Wakabayashi, Masafumi Hirono

{"title":"Chlamydomonas γ-tubulin mutations reveal a critical role for γ-TuRC in maintaining the stability of centriolar microtubules.","authors":"Yuki Nakazawa, Naoto Kubota, Mao Horii, Akira Noga, Yoshikazu Koike, Hiroko Kawai-Toyooka, Hideo Dohra, Katsushi Yamaguchi, Shuji Shigenobu, Ken-Ichi Wakabayashi, Masafumi Hirono","doi":"10.1242/bio.062609","DOIUrl":"https://doi.org/10.1242/bio.062609","url":null,"abstract":"The centriolar triplet microtubule consists of an A-tubule with 13 protofilaments, and B- and C-tubules, each with 10 protofilaments. Although the formation of the triplets has been shown to require γ-tubulin, its specific role in the formation of each tubule remains elusive. We isolated two novel Chlamydomonas reinhardtii mutants, bld13-1 and bld13-2, each expressing γ-tubulin with a single amino-acid substitution (T292I or E89D). Similar to known centriole-deficient mutants, both mutants exhibited defects in ciliary assembly, nuclear number, as well as the number and orientation of cytoplasmic microtubules. Genetic analyses of the mutants, along with the expression of the mutant γ-tubulins in the wild-type cells, suggested that both mutants exert dominant-negative effects over wild-type γ-tubulin. Interestingly, although the centrioles in these mutants retained the typical nine triplet structure, their triplets frequently lacked several protofilaments in specific regions of the A- and C-tubules. The protofilament loss occurs more frequently in the proximal region of the centriole. These structural defects suggest a critical role for γ-tubulin in maintaining the stability of the A- and C-tubules of centriolar triplets.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149165122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental evidence that dietary antioxidants induce an antiviral transcriptomic signature in a wild seabird. 实验证据表明，饮食抗氧化剂诱导野生海鸟抗病毒转录组特征。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-10-12 DOI: 10.1242/bio.062786

Manrico Sebastiano, Marcel Eens, Olivier Chastel, Louis Maigné, David Costantini

{"title":"Experimental evidence that dietary antioxidants induce an antiviral transcriptomic signature in a wild seabird.","authors":"Manrico Sebastiano, Marcel Eens, Olivier Chastel, Louis Maigné, David Costantini","doi":"10.1242/bio.062786","DOIUrl":"https://doi.org/10.1242/bio.062786","url":null,"abstract":"Infectious disease emergence in natural systems is driven by complex host-pathogen-environment interactions. Nutritional factors, particularly dietary antioxidants, can modulate physiological states by enhancing immune function and mitigating oxidative damage, but the underlying molecular mechanisms in wildlife remain poorly understood. To bridge this gap, we conducted a transcriptomic analysis of blood samples from both healthy and clinically affected Magnificent frigatebirds Fregata magnificens chicks treated with either resveratrol (a plant-derived polyphenol with known antiviral and antioxidant properties) or a placebo. Resveratrol supplementation induced significant gene expression changes, particularly in diseased individuals, where we identified the upregulation of ten key genes in treated sick chicks, of which two are unannotated novel transcripts, including the interferon-stimulated genes (IFI6, OASL2) and immune regulators (SOCS1, PLAC8). Furthermore, resveratrol treatment was associated with increased expression of DDX60, a potent antiviral factor, and CYGB1, which supports cytoprotection. In contrast, healthy chicks showed fewer differentially expressed genes, primarily associated with metabolism and cellular organization. Collectively, our results reveal a transcriptional signature consistent with dietary antioxidants such as resveratrol acting as active molecular modulators of host defence, rather than simple passive antioxidants, although direct evidence of enhanced antiviral protection (e.g. reduced viral load) remains to be established. This study highlights the utility of transcriptomic approaches to link nutritional ecology with disease dynamics, offering a framework for understanding how dietary quality shapes host defence strategies and population resilience under increasing pathogen pressure.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear blebs are composed of variable chromatin states and display increased transcription initiation relative to elongation. 核泡由可变的染色质状态组成，并表现出相对于伸长增加的转录起始。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-10-12 DOI: 10.1242/bio.062585

Madeleine Clark, Antonela Losada, Sonia Jahng, Allen Saini, Fawwaz Chowhan, Gwyneth Woods, Adelaide Cutler, Stephanie Hallerman, Minnah Gayed, Sayali Bhalerao, Emanuel Bullock, Chris Santry, Adriana Panagiotou, Bailey Lapolla, Nitish Bhatta, Savanah sFreidus, Gurnoor Kaur, David Bai, Daniel Hu, Kian Tadbiri, Mary Packard, Katherine Dorfman, Nickolas Borowski, Kelsey Prince, Nick Lang, Carline Fermino do Rosario, Andrew D Stephens

{"title":"Nuclear blebs are composed of variable chromatin states and display increased transcription initiation relative to elongation.","authors":"Madeleine Clark, Antonela Losada, Sonia Jahng, Allen Saini, Fawwaz Chowhan, Gwyneth Woods, Adelaide Cutler, Stephanie Hallerman, Minnah Gayed, Sayali Bhalerao, Emanuel Bullock, Chris Santry, Adriana Panagiotou, Bailey Lapolla, Nitish Bhatta, Savanah sFreidus, Gurnoor Kaur, David Bai, Daniel Hu, Kian Tadbiri, Mary Packard, Katherine Dorfman, Nickolas Borowski, Kelsey Prince, Nick Lang, Carline Fermino do Rosario, Andrew D Stephens","doi":"10.1242/bio.062585","DOIUrl":"https://doi.org/10.1242/bio.062585","url":null,"abstract":"Nuclear blebs are herniations of the nucleus that occur in many human conditions including aging, heart disease, muscular dystrophy, and many cancers. Nuclear blebbing causes nuclear rupture and cellular dysfunction. However, understanding the formation, stability, and identification of nuclear blebs remains an ongoing challenge. Our previous studies reveal that nuclear blebs are best hallmarked by decreased DNA density. To determine if chromatin decompaction underlies decreased DNA density in nuclear blebs, we investigated the histone composition of nuclear blebs across multiple cell lines. Time lapse and immunofluorescence imaging revealed that histone levels decreased in the nuclear bleb compared to the nuclear body but were more variable than DNA. Next, we imaged histone modification states of euchromatin and heterochromatin, which respectively track decompact and compact states of chromatin. We find that nuclear blebs display variable histone modification state across cell lines, as euchromatin does not consistently enrich nor is heterochromatin consistently depleted. Nuclear blebs did consistently show active RNA Pol II initiation is significantly increased relative to elongation. Thus, decreased DNA density that hallmarks nuclear blebs is not due to local histone modification state and transcription initiation increased relative to elongation is reproducible in nuclear blebs.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149165276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and autofluorescence of the thallus of the golden-brown alga Hydrurus foetidus by optical and fluorescence-lifetime imaging. 用光学和荧光寿命成像技术观察金褐藻胎水螅体的形态和自身荧光。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-10-07 DOI: 10.1242/bio.062873

Jens Ådne Rekkedal Haga, Live Lislerud Vestli, Kjetill Sigurd Jakobsen, Julien Alain Andre Resseguier, Felix Margadant, Oddmund Bakke, Xian Hu

{"title":"Morphology and autofluorescence of the thallus of the golden-brown alga Hydrurus foetidus by optical and fluorescence-lifetime imaging.","authors":"Jens Ådne Rekkedal Haga, Live Lislerud Vestli, Kjetill Sigurd Jakobsen, Julien Alain Andre Resseguier, Felix Margadant, Oddmund Bakke, Xian Hu","doi":"10.1242/bio.062873","DOIUrl":"https://doi.org/10.1242/bio.062873","url":null,"abstract":"The chrysophyte Hydrurus foetidus belongs to the psychrophilic, rheophilic algae of cold, fast-flowing glacial and alpine streams but remains poorly characterised at the cellular level. We present a systematic optical-microscopy study of field-collected material of this organism, combining confocal imaging, quantitative morphometry, three-dimensional volumetry and fluorescence-lifetime imaging (FLIM). Confocal imaging revealed a pseudoparenchymatous architecture: elongated, longitudinally aligned cells dominate the central axis, whereas round cells occupy the peripheral branches, and the two populations differ in volume. High-magnification imaging showed a consistent apical bias in nuclear position along the cell files of the central axis. FLIM of endogenous autofluorescence yielded reproducible lifetime signatures across the green, orange and far-red emission channels; in the far-red the chlorophyll a signal resolved into a two-component mixture, and the round-cell population was bimodal in lifetime whereas the elongated population was not. All material derives from a single winter collection; the whole-thallus spatial analyses come from one intact thallus, while morphometry, volumetry and FLIM sampled cells from multiple thalli of that collection, and the data are analysed accordingly. Together, these data provide a morphological and photophysical foundation, and a label-free autofluorescence reference, for future cell-biological study of H. foetidus and of multicellular organisation among the chrysophytes.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultraviolet radiation exposure during larval development is associated with changes in colouration of Green and Golden Bell Frogs (Ranoidea aurea). 幼虫发育过程中暴露在紫外线下与绿铃蛙和金铃蛙（Ranoidea aurea）的颜色变化有关。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-10-02 DOI: 10.1242/bio.062793

Ewan S Auld, Aimee J Silla, John A Endler, Phillip G Byrne

{"title":"Ultraviolet radiation exposure during larval development is associated with changes in colouration of Green and Golden Bell Frogs (Ranoidea aurea).","authors":"Ewan S Auld, Aimee J Silla, John A Endler, Phillip G Byrne","doi":"10.1242/bio.062793","DOIUrl":"https://doi.org/10.1242/bio.062793","url":null,"abstract":"Ultraviolet (UV) radiation can be harmful to amphibians by causing DNA damage, impairing immune systems and restricting growth. In response to elevated UV, amphibians have been observed to invest in skin darkening for photoprotection. However, it is unclear whether investment in pigmentation persists across life stages, or whether there are other carry-over effects of UV exposure on amphibian colouration. Here, we experimentally investigate the effects of larval UV exposure on larval and post-metamorphic colouration of the threatened Green and Golden Bell Frog (Ranoidea aurea). UV exposure was provided via two treatments: low UV (∼0.3 - 0.9 UVI) and high UV (∼0.8 - 2.6 UVI), both within the intensity range in nature. Over the experimental period, colouration was measured at weeks 4, 6, and 8, and then immediately post-metamorphosis. As expected, larvae reared under higher UV exhibited stronger skin darkening up until metamorphosis. This trend persisted post-metamorphosis, with metamorphs from the high UV treatment exhibiting darker colouration. Metamorphs from the high UV treatment also displayed a higher proportion of gold colouration that was long-wave shifted in hue and lower in chroma. Collectively, these findings provide some of the first evidence that UV exposure has carry-over effects on amphibian colouration.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical fragility in the meta-analytic evidence base of animal cognition: A meta-meta-analytic reappraisal. 动物认知的元分析证据基础中的统计脆弱性：一个元分析的再评价。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-09-28 DOI: 10.1242/bio.062841

Ayumi Mizuno, Pietro Pollo, Malgorzata Lagisz, Lauren Guillette, Masayo Soma, Shinichi Nakagawa

{"title":"Statistical fragility in the meta-analytic evidence base of animal cognition: A meta-meta-analytic reappraisal.","authors":"Ayumi Mizuno, Pietro Pollo, Malgorzata Lagisz, Lauren Guillette, Masayo Soma, Shinichi Nakagawa","doi":"10.1242/bio.062841","DOIUrl":"https://doi.org/10.1242/bio.062841","url":null,"abstract":"How reliable is the meta-analytic evidence base in animal cognition? We conducted a pre-registered second-order meta-analysis of 28 published meta-analytical papers, comprising 48 eligible models and 5,740 effect-size observations from primary studies. We assessed statistical power, Type M magnitude error, and Type S sign error at both primary-study and meta-analysis levels. Because these quantities depend on the assumed underlying effect, we compared results based on uncorrected pooled effects, a regression-based bias correction, and a bias-robust FE+VCV sensitivity analysis. At the primary-study level, estimated power was low under all three empirically estimated effect proxies: 17.4%, 9.0%, and 13.4%, respectively. Type M and Type S errors were also sensitive to the assumed effect and correction method. Power increased when larger externally specified effects were assumed, confirming that these quantities are conditional on the assumed effect. Meta-analysis-level results were generally more favourable but sensitive to analytical choices. The evidence base was concentrated in experimental rodent studies of learning and memory, limiting field-wide generalisation. These findings support improving study design and reporting within the represented literature while expanding meta-analytic coverage across taxa and cognitive domains.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early injury responses to nutrient treatment in adult Drosophila limb. 成年果蝇肢体对营养处理的早期损伤反应。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-09-28 DOI: 10.1242/bio.062737

Yutian Li, Jesus Del Rio Salgado, Gloria Bates, Zevin J Condiotte, Maya Caskey, Christian M Dimayuga, Brian Williams, Ingileif Hallgrimsdottir, Lior Pachter, Lea Goentoro

{"title":"Early injury responses to nutrient treatment in adult Drosophila limb.","authors":"Yutian Li, Jesus Del Rio Salgado, Gloria Bates, Zevin J Condiotte, Maya Caskey, Christian M Dimayuga, Brian Williams, Ingileif Hallgrimsdottir, Lior Pachter, Lea Goentoro","doi":"10.1242/bio.062737","DOIUrl":"https://doi.org/10.1242/bio.062737","url":null,"abstract":"It was previously observed that partial regrowth can be induced to begin in adult Drosophila limb by modulating nutrients. In this work, to further investigate the observation, we characterized the early cellular and tissue-level processes that are promoted by nutrient treatment after injury. We find that response to nutrient treatment can be readily identified within hours, by alteration in wound healing. Subsequently, at day-scale, dramatic reversal of large-scale tissue loss occurs in treated flies. Finally, at week-scale, tissue remodeling begins and a dome-shaped protrusion forms at the injury site. Performing single-nucleus RNA sequencing at this stage, we find widespread transcriptional modulations across cell types. Distinct muscle and epithelial cell types emerge that are enriched in mitotic and morphogenetic genes, including those associated with regeneration in larval tissues. The tissue-, cell-, and gene-level processes characterized here provide a framework for the previously observed partial limb growth and for further investigating the suggestion of regeneration induction in adult Drosophila by nutrient treatment.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0