Biology Open最新文献_第2页

Coincident evolution and functional adaptation of the taxonomically-restricted genes ivph-3 and gon-14 in Caenorhabditis nematodes. 线虫分类限制性基因ivph-3和gon-14的同步进化和功能适应。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-25 DOI: 10.1242/bio.062018

Nikita Jhaveri, Bhagwati Gupta, Helen M Chamberlin

引用次数: 0

Saccharomyces cerevisiae malate dehydrogenase Mdh1p lacking mitochondrial targeting signal can be re-localized to peroxisomes. 缺乏线粒体靶向信号的酿酒酵母苹果酸脱氢酶Mdh1p可以重新定位到过氧化物酶体上。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-25 DOI: 10.1242/bio.062199

Chutima Chan, Naraporn Sirinonthanawech, Brian K Sato, James E Wilhelm, Chalongrat Noree

{"title":"Saccharomyces cerevisiae malate dehydrogenase Mdh1p lacking mitochondrial targeting signal can be re-localized to peroxisomes.","authors":"Chutima Chan, Naraporn Sirinonthanawech, Brian K Sato, James E Wilhelm, Chalongrat Noree","doi":"10.1242/bio.062199","DOIUrl":"10.1242/bio.062199","url":null,"abstract":"Yeast mitochondrial malate dehydrogenase, Mdh1p, is known to form supramolecular complexes with other tricarboxylic acid (TCA) cycle and mitochondrial dehydrogenase enzymes, including the aldehyde dehydrogenase, Ald4p. These complexes have been proposed to facilitate NADH channeling. Here, we demonstrate that in cells grown to saturation and stationary phases, the endogenous Mdh1p, expressed without its mitochondrial targeting signal (MTS), stays outside mitochondria, in both a diffuse cytoplasmic distribution and localized to distinct puncta. The puncta formed by MTS-lacking Mdh1p show no co-localization with the MTS-lacking Ald4p, suggesting that they do not co-assemble into a supramolecular complex in the cytoplasm. However, we found that the MTS-lacking Mdh1p does co-localize with its cytoplasmic counterpart, Mdh2p, in puncta. Interestingly, Mdh2p has recently been reported to form heterocomplexes with the peroxisomal Mdh3p and to be transported into peroxisomes to assist in the glyoxylate cycle. We also show that the MTS-lacking Mdh1p co-localizes with a fluorescent peroxisome marker, Pex3p. Our findings suggest that different malate dehydrogenases can enter peroxisomes, potentially as a means to make the glyoxylate pathway more efficient.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids. 利用薄片荧光显微镜和卤代烃基液体对蜜蜂胚胎进行长期荧光实时成像。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-26 DOI: 10.1242/bio.062151

Oksana Netschitailo, Paul Siefert, Markus M Middeke, Artemiy Golden, Christin Schrod, Tim Beringer, Bernd Grünewald, Ernst H K Stelzer, Frederic Strobl

{"title":"Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids.","authors":"Oksana Netschitailo, Paul Siefert, Markus M Middeke, Artemiy Golden, Christin Schrod, Tim Beringer, Bernd Grünewald, Ernst H K Stelzer, Frederic Strobl","doi":"10.1242/bio.062151","DOIUrl":"10.1242/bio.062151","url":null,"abstract":"The eusocial honeybee Apis mellifera is a key pollinator and model for insect development, offering insights into the evolutionary history of holometabolous insects. Honeybee embryos develop in a controlled hive environment, which has led to potential adaptations in embryogenesis compared to solitary insect species such as the fruit fly Drosophila melanogaster. However, previously applied static imaging techniques are not well-suited to study morphogenic events. Here, we combine mRNA-based transient fluorescence labeling, Perfluorodecalin as the imaging medium, and a custom sample chamber for light sheet fluorescence microscopy to enable long-term live imaging of honeybee embryos from blastoderm formation to hatching. Our approach provides the first dynamic visualization of extra-embryonic membrane formation in honeybees, which exhibits variable window closure locations at the posterior-ventral area of the embryo. This contrasts with the red flour beetle Tribolium castaneum, where the serosa window closes at a confined anterior-ventral area. Taken together, our methodological framework expands the toolkit for alternative insect models, enabling comparative studies and investigations of environmental stressors, such as pesticides, on development. Plasmids used and datasets acquired in this study are publicly available, supporting future studies on insect diversification and conservation.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"14 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of mice with combined Hexa Gly269Ser KI or KO and Neu3 KO alleles to create new models of GM2 gangliosidoses. 培养具有Hexa Gly269Ser KI或KO和Neu3 KO等位基因的小鼠，以建立新的GM2神经节苷脂剂量模型。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-30 DOI: 10.1242/bio.062045

Emily N Barker, Mehrafarin Ashiri, Jennifer T Saville, Richard Hemming, Nikolas Furletti, Shreya H Dhume, Shirley Yu, Elaine Anjos, Xiaoli Wu, Agnes Fresnoza, David C Merz, Mike Jackson, Marc R Del Bigio, Tabrez J Siddiqui, Maria Fuller, Brian L Mark, Barbara Triggs-Raine

{"title":"Generation of mice with combined Hexa Gly269Ser KI or KO and Neu3 KO alleles to create new models of GM2 gangliosidoses.","authors":"Emily N Barker, Mehrafarin Ashiri, Jennifer T Saville, Richard Hemming, Nikolas Furletti, Shreya H Dhume, Shirley Yu, Elaine Anjos, Xiaoli Wu, Agnes Fresnoza, David C Merz, Mike Jackson, Marc R Del Bigio, Tabrez J Siddiqui, Maria Fuller, Brian L Mark, Barbara Triggs-Raine","doi":"10.1242/bio.062045","DOIUrl":"10.1242/bio.062045","url":null,"abstract":"The GM2 gangliosidoses are lysosomal storage disorders exhibiting a spectrum of neurological phenotypes ranging from childhood death to debilitating adult-onset neurological impairment. To date, no mouse model harbouring a specific human mutation causing GM2 gangliosidosis has been created. We used CRISPR/Cas9 to generate knockin (KI) mice with the common adult-onset Hexa Gly269Ser variant as well as knockout (KO) mice with Hexa mutations expected to cause complete HexA deficiency. We also created Neu3 KO alleles that combined with Hexa KO or KI alleles were expected to create acute and chronic models of GM2 gangliosidosis, respectively. However, both models accumulated GM2 ganglioside throughout the brain when compared to controls (CON), and exhibited progressive loss of reflexes, gait abnormalities, and premature death by 24 weeks of age. Although survival and behavioural phenotypes did not differ between KO and KI models, the KI model had substantial Hexa mRNA and evidence of GM2 turnover. This KI model will be useful for developing gene editing to correct the variant causing the Gly269Ser substitution and its novel biochemical phenotype suggests it may be suitable for testing therapies that treat partial β-hexosaminidase A deficiency.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epithelial fusion is mediated by a partial epithelial-mesenchymal transition. 上皮融合是由部分上皮-间质转化介导的。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-26 DOI: 10.1242/bio.062213

Varsha N Tamilkumar, Harsha Purushothama, Raj K Ladher

{"title":"Epithelial fusion is mediated by a partial epithelial-mesenchymal transition.","authors":"Varsha N Tamilkumar, Harsha Purushothama, Raj K Ladher","doi":"10.1242/bio.062213","DOIUrl":"10.1242/bio.062213","url":null,"abstract":"Epithelial fusion is a fundamental morphogenetic process critical for the closure and compartmentalisation of developing organs. While widely studied in systems such as neural tube and palatal closure, the cellular transitions that enable fusion remain poorly understood. Here, we investigate epithelial fusion during chick otic vesicle closure and identify a transient population of cells at the epithelial interface that mediate this process. These otic epithelial edge (OE) cells exhibit distinct morphology, reduced apicobasal polarity, and dynamic junctional remodelling, including altered distribution of ZO-1, CDH1 and RAC1. Notably, OE cells lack basal contact and display high sphericity, consistent with a partial epithelial-to-mesenchymal transition (EMT) phenotype. Transcriptomic profiling of microdissected tissues reveals that OE cells constitute a transcriptionally distinct population, enriched for EMT regulators, extracellular matrix remodelling genes, and WNT pathway components. Among these, the transcription factors Grhl2 and Sp8 were specifically expressed at the OE and exhibited opposing roles in epithelial identity. CRISPR-Cas9-mediated knockdown of either gene led to disrupted CDH1 localisation, loss of OE cell morphology and failure in epithelial segregation. These results suggest that epithelial fusion requires a regulated, hybrid EMT state that balances junctional plasticity with tissue cohesion. Our findings demonstrate that fusion-competent epithelial cells are not merely passive participants but actively modulate their shape, polarity, adhesion and genetic identity to enable morphogenesis.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking peak intensity of mechanically stimulated bioluminescence and cell surface area in dinoflagellates. 鞭毛藻中机械刺激生物发光的峰值强度与细胞表面积的联系。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-19 DOI: 10.1242/bio.062190

Francis Letendre, Abigail Blackburn, Michael Twardowski

{"title":"Linking peak intensity of mechanically stimulated bioluminescence and cell surface area in dinoflagellates.","authors":"Francis Letendre, Abigail Blackburn, Michael Twardowski","doi":"10.1242/bio.062190","DOIUrl":"10.1242/bio.062190","url":null,"abstract":"Mechanically stimulated bioluminescence (MSL) is present in most planktonic clades and marine ecosystems. The first flash kinetic parameters (FFKPs) and spectral properties are often species specific, making MSL a powerful tool for in situ ID and biodiversity assessments. The peak intensity (PI) of mechanically stimulated bioluminescence was measured for five species of dinoflagellates: Alexandrium monilatum, Lingulodinium polyedra, Pyrocystis fusiformis, Pyrocystis noctiluca and Pyrodinium bahamense. Peak intensity was assessed with respect to organism cell surface area and volume, building upon Seliger's rule, where previously a relationship was found linking cell surface area and total mechanically stimulated light (TMSL) ( Buskey and Swift, 1990). These dinoflagellate species were chosen to cover a wide range of peak intensities (108 to 1010 photons/s) and surface area (104 to 106μm2). Individual cells were isolated and individually photographed under a compound microscope, where cell size was measured. They were then dark-adapted and first flash emission from mechanical stimulation was measured with the Underwater Bioluminescence Assessment Tool (UBAT) from Seabird Scientific (www.seabird.com). Distributions of PI and surface area across all species were compared using non-parametric ANOVAs and a linear regression model, uncovering a strong positive correlation and strength of fit across all species between peak intensity and both cell surface area and volume. This study provides insight into understanding and potentially predicting the bioluminescence of organisms often responsible for significant primary and secondary productivity in marine waters with subsequent global impacts on fisheries and ecology. Bioluminescence measurements may also be a powerful tool for understanding plankton composition, ecology, and diversity.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem cell-specific NF-κB is required for stem cell survival and epithelial regeneration upon intestinal damage. 干细胞特异性NF-κB是肠损伤后干细胞存活和上皮再生所必需的。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-18 DOI: 10.1242/bio.062025

Aurélia Joly, Meghan Ferguson, Minjeong Shin, Edan Foley

引用次数: 0

Interactions of husbandry, landscape, and immunity in regulating viral loads for managed honey bees. 饲养、景观和免疫在调节管理蜜蜂病毒载量中的相互作用。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-22 DOI: 10.1242/bio.062201

Allison Malay, Rachel Weavers, Kenneth M Fedorka

{"title":"Interactions of husbandry, landscape, and immunity in regulating viral loads for managed honey bees.","authors":"Allison Malay, Rachel Weavers, Kenneth M Fedorka","doi":"10.1242/bio.062201","DOIUrl":"10.1242/bio.062201","url":null,"abstract":"The western honey bee, Apis mellifera, continues to experience widespread die-offs that threaten their critical ecological and agricultural roles. Given the recognized impact of viruses on the increased mortality rates, it is imperative to understand the forces shaping viral infections. In this study, we explore how hive husbandry, landscape, and immunity influence viral loads in managed bees. We characterized 43 apiaries across Central Florida for eight husbandry interventions, five landscape variables, transcription of four immune genes, and infection intensities of four viruses: Black Queen cell virus (BQCV), deformed wing virus type A (DWV-A), Lake Sinai virus (LSV-2), and Israeli acute paralysis virus (IAPV). We found that colonies surrounded by more floral resources and fresh water bodies were associated with increased viral loads and increased viral coinfections. We speculate that increased floral resources increased pollinator abundance, thereby increasing transmission rates and viral richness. We further speculate that increased open water similarly increased pollinator abundance and/or exposure to immunity-altering pesticides. Last, we show that husbandry interventions aimed at reducing Varroa destructor mites can have positive and negative off-target viral impacts. Our data underscore the importance of landscape, immunity, and husbandry in honey bee disease dynamics and highlight the complexity of their interactions.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physical confinement and phagocytic uptake induce persistent cell migration. 物理限制和吞噬摄取诱导持续的细胞迁移。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-17 DOI: 10.1242/bio.062021

Summer G Paulson, Sophia Liu, Jeremy D Rotty

{"title":"Physical confinement and phagocytic uptake induce persistent cell migration.","authors":"Summer G Paulson, Sophia Liu, Jeremy D Rotty","doi":"10.1242/bio.062021","DOIUrl":"10.1242/bio.062021","url":null,"abstract":"Physical confinement is not routinely considered as a factor that influences phagocytosis, which is typically investigated using unconfined in vitro assays. BV2 microglia-like cells were used to interrogate the impact of confinement on IgG-mediated phagocytosis side by side with unconfined cells. Confinement acted as a potent phagocytic driver, greatly increasing the fraction of phagocytic cells in the population compared to the unconfined setting. Arp2/3 complex and myosin II contributed to this effect. Remarkably, confinement partially rescued phagocytic uptake upon myosin II disruption. In addition, cells under confinement were partially resistant to the actin-depolymerizing drug cytochalasin D. Unexpectedly, we observed that bead uptake stimulated persistent migration, a process we term 'phagocytic priming'. Integrin-dependent adhesion was required for phagocytic priming in unconfined and confined settings but was dispensable for phagocytic uptake. The cytoskeletal requirements for phagocytic priming differed depending on confinement state. Myosin II and Arp2/3 complex were required for phagocytic priming under confinement, but not in unconfined settings. As with phagocytosis, cytoskeleton-dependent priming of motility varies based on physical confinement status. Phagocytic priming may be a crucial innate immune mechanism by which cells respond to wounds or trauma with increased surveillance of the local microenvironment.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invasive goldfish (Carassius auratus) maintain aerobic scope across acute warm water temperatures. 入侵金鱼（Carassius auratus）在急性温暖的水温下维持有氧范围。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-03 DOI: 10.1242/bio.062160

Nazeefa A Nashrah, Nicholas E Mandrak, Melanie D Massey

{"title":"Invasive goldfish (Carassius auratus) maintain aerobic scope across acute warm water temperatures.","authors":"Nazeefa A Nashrah, Nicholas E Mandrak, Melanie D Massey","doi":"10.1242/bio.062160","DOIUrl":"10.1242/bio.062160","url":null,"abstract":"Goldfish (Carassius auratus) were first introduced to the Laurentian Great Lakes when it was first introduced into Lake Ontario in the 1800s. In the past 15 years, there have been dramatic increases in both goldfish abundance and geographic spread across North America, including the Great Lakes, raising concerns about its potential for negative impacts on aquatic ecosystems. Climate studies suggest that habitat ranges suitable for goldfish will continue to expand in the future as water temperatures rise towards its thermal optima. We explore whether warmer temperatures are physiologically suitable for a population of wild, invasive goldfish (Hamilton, ON, Canada) by testing aerobic scope (AS) at current (26°C) and predicted (30°C) peak summer water temperatures. Goldfish were first acclimated to a common-garden average summer pond temperature (22°C), then their AS was estimated by calculating the difference between routine and maximum metabolic rates (RMR and MMR) at the two test temperatures. Our results demonstrate that wild goldfish sustain their AS through increases in both RMR and MMR from 26°C to 30°C (mass-standardized means of 1.07 versus 1.17 mgO2h-1 at 26°C versus 30°C, respectively). This ability to sustain aerobic energy budget at high peak water temperatures could offer physiological benefits to this invasive population in a warming climate.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0