Biology Open最新文献_第2页

Science under siege: protecting scientific progress in turbulent times. 围攻中的科学：在动荡时期保护科学进步。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-13 DOI: 10.1242/bio.061972

James Briscoe, Craig E Franklin, Daniel A Gorelick, E Elizabeth Patton, Michael Way

引用次数: 0

Primed bovine embryonic stem cell lines can be derived at diverse stages of blastocyst development with similar efficiency and molecular characteristics.

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-07 DOI: 10.1242/bio.061819

Carly Guiltinan, Ramon C Botigelli, Juliana I Candelaria, Justin M Smith, Rachel B Arcanjo, Anna C Denicol

{"title":"Primed bovine embryonic stem cell lines can be derived at diverse stages of blastocyst development with similar efficiency and molecular characteristics.","authors":"Carly Guiltinan, Ramon C Botigelli, Juliana I Candelaria, Justin M Smith, Rachel B Arcanjo, Anna C Denicol","doi":"10.1242/bio.061819","DOIUrl":"10.1242/bio.061819","url":null,"abstract":"In this study, we established bovine embryonic stem cell (bESC) lines from early (eBL) and full (BL) blastocysts to determine the efficiency of bESC derivation from an earlier embryonic stage and compare the characteristics of the resulting lines. Using established medium and protocols for derivation of primed bESCs from expanded blastocysts, we derived bESC lines from eBLs and BLs with the same efficiency (4/12 each, 33%). Regardless of original blastocyst stage, bESC lines had a similar phenotype, including differentiation capacity, stable karyotype, and pluripotency marker expression over feeder-free transition and long-term culture. Transcriptome and functional analyses indicated that eBL- and BL-derived lines were in primed pluripotency. We additionally compared RNA-sequencing data from our lines to bovine embryos and stem cells from other recent reports, finding that base medium was the predominant source of variation among cell lines. In conclusion, our results show that indistinguishable bESC lines can be readily derived from eBL and BL, widening the pool of embryos available for bESC establishment. Finally, our investigation points to sources of variation in cell phenotype among recently reported bESC conditions, opening the door to future studies investigating the impact of factors aside from signaling molecules on ESC derivation, maintenance, and performance.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast & Fair peer review: a bold experiment in scientific publishing. 快速公平的同行评议：科学出版领域的一次大胆实验。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-25 DOI: 10.1242/bio.061982

Daniel A Gorelick, Alejandra Clark

引用次数: 0

From fossils to future - Jane Francis on polar exploration and changing climates. 从化石到未来——简·弗朗西斯谈极地探险和气候变化。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-24 DOI: 10.1242/bio.061973

Katie Pickup

引用次数: 0

Deciphering the flapping frequency allometry: unveiling the role of sustained body attitude in the aerodynamic scaling of normal hovering animals.

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-14 DOI: 10.1242/bio.061932

Jeremy Pohly, Chang-Kwon Kang, Hikaru Aono

引用次数: 0

Neurogenetic disorders associated with mutations in the FERRY complex: a novel disease class?

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-10 DOI: 10.1242/bio.061808

R Madison Riffe, Gerald B Downes

{"title":"Neurogenetic disorders associated with mutations in the FERRY complex: a novel disease class?","authors":"R Madison Riffe, Gerald B Downes","doi":"10.1242/bio.061808","DOIUrl":"10.1242/bio.061808","url":null,"abstract":"The five-subunit endosomal Rab5 and RNA/ribose intermediary (FERRY) complex is a newly described protein complex consisting of TBCK, PPP1R21, FERRY3 (previously C12orf4), CRYZL1, and GATD1. The FERRY complex is proposed to function as a Rab5 effector to shuttle mRNA to the cell periphery for local translation, a process especially important in cells with far reaching processes. Interestingly, three members of the FERRY complex are associated with ultra-rare neurogenetic disorders. Mutation of TBCK causes TBCK syndrome, mutation of PPP1R21 is associated with PPP1R21-related intellectual disability, and mutation of FERRY3 results in an autosomal recessive intellectual disability. Neurologic disorders have yet to be associated with mutation of GATD1 or CRYZL1. Here, we provide a review of each FERRY complex-related neurologic disorder and draw clinical comparisons between the disease states. We also discuss data from the current cellular and animal models available to study these disorders, which is notably disparate and scattered across different cell types and systems. Taken together, we explore the possibility that these three diseases may represent one shared disease class, which could be further understood by combining and comparing known information about each individual disease. If true, this could have substantial implications on our understanding of the cellular role of the FERRY complex and on treatment strategies for affected individuals, allowing researchers, clinicians, and patient organizations to maximize the utility of research efforts and resources to support patients with these disorders.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"14 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR mis-insertion in the Zic3 5'UTR inhibits in vivo translation and is predicted to result in formation of an mRNA stem-loop hairpin.

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-17 DOI: 10.1242/bio.061677

Helen M Bellchambers, Maria B Padua, Stephanie M Ware

{"title":"A CRISPR mis-insertion in the Zic3 5'UTR inhibits in vivo translation and is predicted to result in formation of an mRNA stem-loop hairpin.","authors":"Helen M Bellchambers, Maria B Padua, Stephanie M Ware","doi":"10.1242/bio.061677","DOIUrl":"10.1242/bio.061677","url":null,"abstract":"Zic3 loss of function is associated with a range of congenital defects, including heterotaxy and isolated heart defects in humans, as well as neural tube defects, situs anomalies, and tail kinks in model organisms. Here, we describe a novel Zic3ins5V mouse line generated due to a mis-insertion during the CRISPR genome editing process, which altered the Zic3 5'UTR structure. Mice with this insertion developed similar phenotypes to Zic3LacZ null mice, including heterotaxy, isolated heart defects, neural tube defects and tail kinks. Surprisingly, gene expression analysis revealed that the novel Zic3ins5V line displays higher levels of Zic3 mRNA, but western blot analysis confirmed that levels of ZIC3 were greatly reduced in vivo. RNAfold, an RNA secondary structure prediction tool, showed that this mis-insertion may cause the formation of a large stem-loop hairpin incorporating some of the 5'UTR and first exon of Zic3, and the insertion of similar hairpins in a cell-based assay caused the loss of ZIC3 expression. Thus, this mouse line displays a loss of ZIC3 protein consistent with the inhibitory effects of 5'UTR stem-loop hairpin structures.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of vessel noise on beluga (Delphinapterus leucas) call type use: ultrasonic communication as an adaptation to noisy environments?

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-20 DOI: 10.1242/bio.061783

Valeria Vergara, Marie-Ana Mikus, Clément Chion, Dominic Lagrois, Marianne Marcoux, Robert Michaud

{"title":"Effects of vessel noise on beluga (Delphinapterus leucas) call type use: ultrasonic communication as an adaptation to noisy environments?","authors":"Valeria Vergara, Marie-Ana Mikus, Clément Chion, Dominic Lagrois, Marianne Marcoux, Robert Michaud","doi":"10.1242/bio.061783","DOIUrl":"10.1242/bio.061783","url":null,"abstract":"Animal vocalizations can evolve structural features as long-term adaptations to noisy environments. Using such signals, cetaceans could mitigate masking from vessel noise. This study investigates whether beluga whales (Delphinapterus leucas) use ultrasonic high-frequency burst pulse (HFBP) calls to communicate in noisy conditions. We identified HFBP calls in three populations: St Lawrence Estuary, Eastern High Arctic-Baffin Bay, and Western Hudson Bay. Focusing on the industrialized St Lawrence, we investigated the effects of vessel noise on HFBP call rates compared to other call types. Ultrasonic calls, spanning a bandwidth of 36.4±6.5 to 144 kHz (Nyquist frequency), comprised 13% of the St Lawrence beluga repertoire (n=25,435). Noise events (n=21) were defined as periods when at least one vessel was visible within 2 km of the hydrophone while belugas were within 500 m. Sound pressure levels were measured before, during, and after exposure. Generalized linear mixed models revealed consistent HFBP call rates before, during, and after vessel noise exposure, while contact calls and other call types declined during exposure (n=4528). These findings suggest that ultrasonic signals that evolved in the Arctic - where ice-associated noise may have created a need for high-frequency communication - remain a viable communication channel in vessel noise, allowing belugas to exploit these signals to maintain communication. Understanding how belugas use signals in noisy environments can inform conservation strategies for noise-impacted marine mammals.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential effect of ubiquitous and germline depletion of Integrator complex function on C. elegans physiology.

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-12 DOI: 10.1242/bio.061930

Brandon M Waddell, Alice Ronita Roy, Carlos Zapien Verdugo, Cheng-Wei Wu

{"title":"Differential effect of ubiquitous and germline depletion of Integrator complex function on C. elegans physiology.","authors":"Brandon M Waddell, Alice Ronita Roy, Carlos Zapien Verdugo, Cheng-Wei Wu","doi":"10.1242/bio.061930","DOIUrl":"https://doi.org/10.1242/bio.061930","url":null,"abstract":"The Integrator is a metazoan-conserved protein complex with endonuclease activity that functions to cleave various RNA substrates to shape transcriptome homeostasis by coordinating small nuclear RNA biogenesis to premature transcription termination. Depletion of Integrator results in developmental defects across different model systems and has emerged as a causative factor in human neurodevelopmental syndromes. Here, we use the model system Caenorhabditis elegans to enable studying the temporal effects of Integrator depletion on various physiological parameters with the auxin-inducible degron system that permitted depletion of INTS-4 (Integrator subunit) catalytic subunit of the protein complex. We found that Integrator activity is critical and required for C. elegans development within the L1 larval stage, but becomes dispensable for development and lifespan after the animals have reached the L2/L3 stage. Depletion of INTS-4 only shortened lifespan if auxin was introduced at the L1 stage, suggesting that the previously described lifespan reduction by Integrator inhibition is linked to developmental growth defects. We also found that while germline-specific degradation of Integrator results in the accumulation of misprocessed snRNA transcript, it did not impair the development or lifespan but surprisingly increased progeny production. Together, our study illustrates a temporal, and a potential tissue-specific requirement of the Integrator complex function in shaping whole organism development, aging, and reproduction.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental short-term heatwaves negatively impact body weight gain and survival during larval development in Bombus terrestris L. (Hymenoptera: Apidae). 实验性短期热浪对地蜂幼虫发育过程中体重增加和存活率有负面影响。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-06 DOI: 10.1242/bio.061781

Laura Wögler, Christoph Kurze

{"title":"Experimental short-term heatwaves negatively impact body weight gain and survival during larval development in Bombus terrestris L. (Hymenoptera: Apidae).","authors":"Laura Wögler, Christoph Kurze","doi":"10.1242/bio.061781","DOIUrl":"https://doi.org/10.1242/bio.061781","url":null,"abstract":"Climate change-induced heatwaves threaten global biodiversity, including crucial pollinators like bumblebees. In particular, the increasing frequency, duration and intensity of heatwaves is alarming. Despite these projections, little is known about the effects of short-term heatwaves on insect larval development. Hence, we investigated the impact of simulated heatwaves on the development of 4th instar larvae (L4) of Bombus terrestris L. (Hymenoptera: Apidae) using an in vitro rearing method. Individual larvae were incubated at 37°C and 38°C for a period of 4 days, with a constant rearing temperature of 34°C as the control. We examined body weight gain, developmental duration, survival to adult stage, and adult body size (i.e. dry mass, intertegular distance, and head width). A simulated heatwave of 37°C did not significantly affect larval development, but 38°C impaired larval body mass gain. While developmental duration and adult body size were unaffected, an acute heat stress of 38°C during the L4 stage reduced the probability of pupae reaching adulthood. These findings highlight the potential for heatwaves to negatively affect bee populations by impairing larval growth and reducing survival to the adult stage, which may have severe implication for colony fitness.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0