Biology Open最新文献_第3页

Mass loss, timing and duration of catastrophic moult in little penguins. 小企鹅灾难性换羽的大量损失、时间和持续时间。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-02 DOI: 10.1242/bio.061989

Naomi C A Wells, Maddison J Ledwidge, Peter Dann, Melissa J Walker, John P Y Arnould

{"title":"Mass loss, timing and duration of catastrophic moult in little penguins.","authors":"Naomi C A Wells, Maddison J Ledwidge, Peter Dann, Melissa J Walker, John P Y Arnould","doi":"10.1242/bio.061989","DOIUrl":"10.1242/bio.061989","url":null,"abstract":"Feather regeneration is vital for birds' thermoregulation, courtship, breeding, camouflage, and locomotion, with strategies reflecting life history. Little penguins (Eudyptula minor) undergo catastrophic moult, replacing all feathers within a short timeframe while on land and not foraging. This study examined the 2015 and 2016 moult seasons on Phillip Island to explore factors influencing moult timing, duration, and mass. Moult started 9.6 days earlier in 2016 (∼Feb. 15) than in 2015 (∼Feb. 24), and year was found to be the only significant predictor of this moult start date. Moult duration was similar between years (medians: 18.0 days in 2015, 17.5 in 2016) and only slightly reduced with later start dates (-0.04 days per day delay; ∼58 min). Average daily mass loss during moult were best explained by moult duration and starting mass, with longer moult and greater starting mass leading to greater mass loss. The timing and duration of little penguins' moult, along with the need for significant pre-moult mass gain, are likely influenced by external factors like local prey availability. Moult plasticity likely benefits little penguin survival.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Steroid 21-hydroxylase deficiency dysregulates essential molecular pathways of metabolism and energy provision. 类固醇21-羟化酶缺乏症使代谢和能量供应的基本分子途径失调。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-08 DOI: 10.1242/bio.061977

Irina Bacila, Lara Oberski, Nan Li, Karl-Heinz Storbeck, Vincent T Cunliffe, Nils Krone

{"title":"Steroid 21-hydroxylase deficiency dysregulates essential molecular pathways of metabolism and energy provision.","authors":"Irina Bacila, Lara Oberski, Nan Li, Karl-Heinz Storbeck, Vincent T Cunliffe, Nils Krone","doi":"10.1242/bio.061977","DOIUrl":"10.1242/bio.061977","url":null,"abstract":"The prevalence of metabolic disease is increased in congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency. However, the underlying molecular mechanisms causing these problems are not fully understood. We aimed to elucidate the metabolic phenotype and conduct a transcriptomic analysis of a 21-hydroxylase-deficient zebrafish model, to unravel the molecular mechanisms underlying the metabolic pathophysiology of CAH. The morphology, anatomy and transcriptomic analysis of whole larvae, adult liver tissue from 18-month-old cyp21a2-/- zebrafish were compared to those of wild-type siblings. Our main phenotypical finding was that adult mutants were larger, with increased fat deposition compared to controls, in-keeping with the transcriptomic analysis showing the dysregulation of several biological processes involved in lipid metabolism. Importantly, we found that ATP synthesis and provision of energy precursors were included among the most significantly suppressed processes in both larvae and adult livers. We conclude that cortisol deficiency in cyp21a2-/- mutants causes growth and body fat abnormalities at adult stages, as well as transcriptomic dysregulation of metabolic processes, energy homeostasis and inflammatory responses in both larvae and adults. These findings reveal how GC deficiency in zebrafish contributes to the development of the metabolic comorbidities that are similar to those observed in patients with CAH.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal transcriptomic profiling of human three-dimensional neuromuscular co-cultures. 人类三维神经肌肉共培养的时间转录组分析。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-16 DOI: 10.1242/bio.062196

Neha Jadhav Giridhar, Bita Hambrecht, Maren Schenke, Bettina Seeger, Thorsten Bischler, Michael Briese, Patrick Lüningschrör

{"title":"Temporal transcriptomic profiling of human three-dimensional neuromuscular co-cultures.","authors":"Neha Jadhav Giridhar, Bita Hambrecht, Maren Schenke, Bettina Seeger, Thorsten Bischler, Michael Briese, Patrick Lüningschrör","doi":"10.1242/bio.062196","DOIUrl":"10.1242/bio.062196","url":null,"abstract":"The principal organization of mammalian neuromuscular junctions (NMJs) shares essential features across species. However, human NMJs (hNMJs) exhibit distinct structural and physiological properties. While recent advances in stem-cell-based systems have significantly improved in vitro modeling of hNMJs, the extent to which these models recapitulate in vivo development remains unclear. Here, we performed temporal transcriptomic analysis of human three-dimensional (3D) neuromuscular co-cultures, composed of iPSC-derived motoneurons and skeletal muscle engineered from primary myoblasts. We found that the expression pattern follows a temporally coordinated gene expression program underlying NMJ maturation. The model recapitulates transcriptional features of NMJ development, including early myoblast fusion and presynaptic development, followed by a late-stage upregulation of postsynaptic markers and embryonic AChR subunits. Importantly, comparable transcriptional dynamics across two independent hiPSC lines confirm the reproducibility and robustness of this system. This study confirms on a transcriptional level that human 3D neuromuscular co-cultures are a robust and physiologically relevant model for investigating hNMJ development and function.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of protein-protein interactions and monolayer mechanics in tricellulin localization to tricellular tight junctions. 蛋白-蛋白相互作用和单层力学在三胞蛋白定位到三细胞紧密连接中的作用。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-26 DOI: 10.1242/bio.061987

Toiba Mushtaq, Jaakko Lehtimäki, Konstantin Kogan, Johan Peränen, Xionan Liu, Markku Varjosalo, Aki Manninen, Pekka Lappalainen

{"title":"Roles of protein-protein interactions and monolayer mechanics in tricellulin localization to tricellular tight junctions.","authors":"Toiba Mushtaq, Jaakko Lehtimäki, Konstantin Kogan, Johan Peränen, Xionan Liu, Markku Varjosalo, Aki Manninen, Pekka Lappalainen","doi":"10.1242/bio.061987","DOIUrl":"10.1242/bio.061987","url":null,"abstract":"Tricellular tight junctions (tTJs) seal the space between three or more cells in epithelial monolayers. These specialized tight junctions have distinct protein components, including a transmembrane protein tricellulin. However, the mechanisms by which tricellulin localizes specifically to tTJs are incompletely understood. We demonstrate that tricellulin undergoes rapid lateral diffusion along bicellular junctions but is a very stable component of tTJs. BioID proteomics identified several proximity partners of tricellulin, and knockout studies on angulin-1/LSR, occludin and afadin provided evidence that these proteins control tricellulin accumulation to tTJs to different extents and mechanisms. Tricellulin localization was disrupted in afadin and angulin-1/LSR knockout cells, although these proteins did not display similar accumulation to tTJs, suggesting that they contribute to tricellulin localization through indirect or context-dependent mechanisms. Importantly, experiments on mixed cultures revealed that defects of tricellulin localization in occludin knockout cells were affected by the proximity of wild-type cells, and treatment of monolayers with myosin-II inhibitor resulted in displacement of tricellulin from tTJs. These results suggest that, in addition to protein-protein interactions, proper epithelial monolayer mechanics are essential for stabilizing tricellulin at tTJs.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A functional comparison of two transplantable syngeneic mouse models of melanoma: B16F0 and YUMM1.7. 两种可移植的同基因黑色素瘤小鼠模型B16F0和YUMM1.7的功能比较

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-11 DOI: 10.1242/bio.062175

David J Klinke, Alanna Gould, Anika Pirkey, Atefeh Razazan, Wentao Deng

{"title":"A functional comparison of two transplantable syngeneic mouse models of melanoma: B16F0 and YUMM1.7.","authors":"David J Klinke, Alanna Gould, Anika Pirkey, Atefeh Razazan, Wentao Deng","doi":"10.1242/bio.062175","DOIUrl":"10.1242/bio.062175","url":null,"abstract":"The B16 murine melanoma cell lines are considered the gold standard for testing melanoma immunotherapies due to low treatment success rates. However, the clinical relevance of these models has been questioned due to a mutational landscape void of driver mutations typically seen in human melanomas and a tendency to form necrotic cores at high tumor volumes. Creating the YUMM1.7 line addressed these limitations by providing an additional contextually consistent model with a more clinically relevant genetic background. The combined use of both models can generate stronger studies in melanoma immunology and immunotherapy. However, to date, there have been no direct functional comparisons of the characteristics of these two models to inform the design of such studies. To address this, we conducted a series of functional experiments to characterize the kinetics of tumor growth, chemotherapeutic sensitivity, and immunogenicity of these models. We found that the B16F0 model had faster intrinsic tumor growth rates, was more susceptible to lysis by tumor-specific CD8+ T cells, and secreted higher levels of the angiogenic factors VEGF and Ang2. Meanwhile, the YUMM1.7 model was more sensitive to chemotherapeutic treatment, secreted higher levels of chemokines CCL2, CXCL1, and CX3CL1, and showed higher infiltration of lymphocyte and myeloid subsets at the same tumor size. Overall, YUMM1.7 model may be better suited for in vivo studies of mechanisms that require a wider observation window and intervention than the B16F0 model, such as immune response. However, angiogenesis and immunotherapy studies may benefit from a more in-depth comparative analyses of both models.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alpha-synuclein overexpression does not cause vocalization deficits in a mouse model of parkinsonism. 在帕金森小鼠模型中，α -突触核蛋白过表达不会引起发声缺陷。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-12 DOI: 10.1242/bio.062120

Brooke Rodgers, Allison Schaser

{"title":"Alpha-synuclein overexpression does not cause vocalization deficits in a mouse model of parkinsonism.","authors":"Brooke Rodgers, Allison Schaser","doi":"10.1242/bio.062120","DOIUrl":"10.1242/bio.062120","url":null,"abstract":"Voice deficits are common in Parkinson's disease (PD) and significantly impact quality of life by increasing stress, social isolation, and caregiver burden. However, despite this impact, there are currently no treatments that target the underlying pathophysiology of PD in the vocalization system. The goal of this study was to examine the effect of one possible underlying mechanism responsible for the complex voice deficits that exist in PD; overexpression of the protein alpha-synuclein. Results show that overexpression of alpha-synuclein, prior to the development of alpha-synuclein aggregate pathology, does not result in significant vocalization deficits. A small but statistically significant increase in the total number of complex vocalizations was found in mice overexpressing alpha-synuclein compared to wild-type mice, but there were no differences in complexity ratio or any of the other specific vocalization parameters tested. Results provide a critical foundational understanding of the impact of overexpression versus aggregation of alpha-synuclein on voice deficits in PD. Future work will focus on manipulation of alpha-synuclein aggregate pathology, and not overexpression alone, to reduce or eliminate the burden of PD specific voice disorders.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple, fast and inexpensive approach using E. coli to detect and estimate vitamin B12 content in microbial extracts. 一种利用大肠杆菌检测和估计微生物提取物中维生素B12含量的简单、快速和廉价的方法。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-03 DOI: 10.1242/bio.062017

Katarzyna Hencel, Matthew J Sullivan, Alper Akay

{"title":"A simple, fast and inexpensive approach using E. coli to detect and estimate vitamin B12 content in microbial extracts.","authors":"Katarzyna Hencel, Matthew J Sullivan, Alper Akay","doi":"10.1242/bio.062017","DOIUrl":"10.1242/bio.062017","url":null,"abstract":"Vitamin B12 is an essential micronutrient produced only by prokaryotes, and animals must acquire it from their diet. Vitamin B12 is critical for the synthesis of methionine and propionyl-CoA metabolism. In humans, vitamin B12 deficiency has been linked to many disorders, including infertility and developmental abnormalities. The growing trend towards plant-based diets and ageing populations increases the risk of vitamin B12 deficiency, and, therefore, there is an increasing interest in understanding vitamin B12 biology. Accurate approaches for detecting and quantifying vitamin B12 are essential in studying its complex biology, from its biogenesis in Bacteria and Archaea to its effects in complex organisms. Here, we present an approach using the commonly available E. coli methionine auxotroph strain B834 (DE3) and a multi-well spectrophotometer to detect and estimate the levels of vitamin B12 from biological samples at picomolar concentrations. We further show that our method is sufficient to reveal important differences in the production of vitamin B12 from vitamin B12-synthesising bacteria commonly found in the microbiome of wild Caenorhabditis elegans isolates. Our results establish a high-throughput and simple assay platform for detecting and estimating vitamin B12 levels using the E. coli B834 (DE3) strain.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Giving their all for their offspring: physiological trade-offs in an Andean-Patagonian viviparous lizard in response to global warming. 为后代付出一切：安第斯-巴塔哥尼亚胎生蜥蜴对全球变暖的生理权衡。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-22 DOI: 10.1242/bio.062159

Jimena B Fernández, Erika L Kubisch, Fernando Duran, Jorgelina M Boretto

{"title":"Giving their all for their offspring: physiological trade-offs in an Andean-Patagonian viviparous lizard in response to global warming.","authors":"Jimena B Fernández, Erika L Kubisch, Fernando Duran, Jorgelina M Boretto","doi":"10.1242/bio.062159","DOIUrl":"10.1242/bio.062159","url":null,"abstract":"Global warming threatens biodiversity, particularly affecting ectothermic animals, which must seek refuge to avoid overheating when ambient temperatures exceed their critical thresholds. Extended shelter use limits the time for essential activities such as foraging, social interactions, and reproduction, potentially reducing survival and increasing local extinction risk. Viviparous Liolaemids inhabiting cold-temperate Andean regions are considered vulnerable to rising temperatures and are predicted to experience local extinctions this century. We evaluated the effects of thermal restriction on pregnancy outcomes and offspring in the viviparous lizard Liolaemus pictus under two conditions. One group of pregnant females experienced simulated future thermal restrictions (restriction group, RG; n=12), while another group experienced identical laboratory conditions without thermal restrictions (no-restriction group, NRG; n=14). In RG females, 41.7% were removed due to feeding cessation or spontaneous abortions (versus 12.3% in NRG), reflecting the consequences of thermal restriction. The remaining RG females selected lower preferred body temperatures after 2 weeks of experimentation and maintained a stable body condition throughout pregnancy. However, both groups of offspring showed similar body condition and locomotor performance, suggesting physiological compensation by RG females. This physiological plasticity of L. pictus may help buffer the adverse effects of global warming on reproductive success.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PIWIL2 downregulation in colon cancer promotes transposon activity and pro-tumorigenic phenotypes. PIWIL2在结肠癌中的下调促进转座子活性和促肿瘤表型。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 Epub Date: 2025-09-04 DOI: 10.1242/bio.061942

Alyssa Risner, Joyce Nair-Menon, Abhinav Cheedipudi, Joe R Delaney, Vamsi Gangaraju, Antonis Kourtidis

{"title":"PIWIL2 downregulation in colon cancer promotes transposon activity and pro-tumorigenic phenotypes.","authors":"Alyssa Risner, Joyce Nair-Menon, Abhinav Cheedipudi, Joe R Delaney, Vamsi Gangaraju, Antonis Kourtidis","doi":"10.1242/bio.061942","DOIUrl":"10.1242/bio.061942","url":null,"abstract":"Reactivation of transposable elements (TEs) in somatic tissues, particularly of LINE-1, is associated with disease by causing gene mutations and DNA damage. Previous work has shown that the PIWI pathway is crucial for TE suppression in the germline. However, the status and function of this pathway is not well characterized in differentiated somatic cells and there is a lack of consensus on the role of the pathway in somatic tumorigenesis. To shed light on this conundrum, we examined the PIWI pathway in colon cancer through combining bioinformatic analyses and cell-based assays. Shifted weighted annotation network (SWAN) analysis revealed that the pathway experiences significant allelic losses in colon cancer and that PIWIL2, the main catalytic component of the pathway responsible for TE silencing, experiences the highest percent deletions. PIWIL2 is downregulated in colon tumors of advanced stage, nodal metastasis, and in certain subtypes, correlating with poor survival, while it is also downregulated in ulcerative colitis, an inflammatory bowel disease that predisposes to colon cancer. PIWIL2 depletion in colon epithelial Caco2 cells leads to increased anchorage-independent growth, decreased levels of TE-targeting non-canonical piRNAs, increased LINE-1 levels and activity, and in DNA damage, altogether highlighting a tumor-suppressing role of PIWIL2 in the colon.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ecological approach to honeybee olfactory conditioning: challenges and potential for the monitoring of potato virus Y infection. 蜜蜂嗅觉调节的生态学方法：马铃薯Y病毒感染监测的挑战和潜力。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 DOI: 10.1242/bio.061680

Lorenzo Corsico, Thibault P M Costaz, Jolanda Tom, Martin Verbeek, Aria Samimi, Klaas van Rozen

{"title":"An ecological approach to honeybee olfactory conditioning: challenges and potential for the monitoring of potato virus Y infection.","authors":"Lorenzo Corsico, Thibault P M Costaz, Jolanda Tom, Martin Verbeek, Aria Samimi, Klaas van Rozen","doi":"10.1242/bio.061680","DOIUrl":"10.1242/bio.061680","url":null,"abstract":"Potato (Solanum tuberosum L.) is a global crop with a vital role in food security and economic significance in many countries. Potato virus Y (PVY) is one of its major viral threats, causing severe yield and quality losses when not controlled properly. PVY spreads primarily through aphids and infected tubers, and current management relies on insecticides and protective oils. Novel detection methods are needed to identify infected plants accurately at an early stage of plant development, thus reducing pesticide use. Trained honeybees (Apis mellifera L.) can detect specific volatiles emitted by plants infected by viruses like PVY. Using associative conditioning and the proboscis extension reflex, we tested the capacity of harnessed worker bees to distinguish PVY-infected and healthy potato leaves as a first step towards field application. As a whole, the results were impeded by low response levels and no significant result was obtained. However, we were able to show the capability of honeybees to learn and differentiate between two conditioned stimuli (healthy potato leaves versus clean air). Our findings therefore suggest that honeybees, as a globally accessible resource, have the potential to be used as a cost-effective solution in crop health monitoring, with further investigation and protocol refinement needed to achieve accurate PVY detection in agricultural settings.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0