Biology Open最新文献_第4页

Catch up with authors across the history of Biology Open. 赶上作者在生物学开放的历史。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-09-15 DOI: 10.1242/bio.062200

Saanjbati Adhikari

引用次数: 0

Ultraviolet B radiation impairs coral reef fish development. 紫外线B辐射损害珊瑚礁鱼类的发育。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 Epub Date: 2025-08-26 DOI: 10.1242/bio.062107

Adam T Downie, Coen Hird, Rebecca L Cramp, Fabio Cortesi, Craig E Franklin

{"title":"Ultraviolet B radiation impairs coral reef fish development.","authors":"Adam T Downie, Coen Hird, Rebecca L Cramp, Fabio Cortesi, Craig E Franklin","doi":"10.1242/bio.062107","DOIUrl":"10.1242/bio.062107","url":null,"abstract":"Loss of structural habitat complexity associated with habitat degradation in marine systems may expose early life stages of fishes to harsh environmental conditions. Specifically, loss of coral cover means less suitable refuge is available for some reef fish species to lay their eggs, exposing them to pervasive stressors such as ultraviolet radiation (UVR). Here, using laboratory experiments, we exposed embryos of the clownfish Amphiprion ocellaris for 2 h daily to two UVR levels reflective of their depth at settlement; high UVR (280 µW m-2), reflective of shallow depths, and low UVR (80 µW m-2), reflective of deeper depths over their embryonic period, and then measured changes in mass, yolk sac volume, DNA damage, and survival. Despite being exposed to ecologically relevant levels of UV radiation, there was 100% mortality before hatching and inflated yolk sacs in both high and low UVR-treated animals. Exposure to UVR also resulted in DNA damage, albeit only in high UVR treatments. It is evident from our results that the protection that the reef can offer from UVR is critical for the survival of clownfish. Our results also underscore the need for future work to consider this often-neglected stressor and the role of adequate refuge for the healthy development of early-life stages of reef organisms.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Powered-gliding/climbing flight performed by bats for saving fuel. 动力滑翔/爬升飞行由蝙蝠执行，以节省燃料。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 Epub Date: 2025-09-01 DOI: 10.1242/bio.061779

Gottfried Sachs

{"title":"Powered-gliding/climbing flight performed by bats for saving fuel.","authors":"Gottfried Sachs","doi":"10.1242/bio.061779","DOIUrl":"10.1242/bio.061779","url":null,"abstract":"Results of recent research show that bats perform flights with continual altitude changes rather than flying at a constant altitude. However, the current state of knowledge suggests that the reason for these altitude changes is not known, and it is stated in the literature that further study is necessary in order to understand this behaviour. The goal of this paper is to provide an explanation by showing that flights with continual altitude changes constitute a fuel-saving flight mode in bats. The descents in the altitude changes - which were analysed using flight measurement data - show a power support by flapping the wings to yield a powered glide. Accordingly, this flight mode may be termed powered-gliding/climbing flight. Corresponding to the described flight characteristics, powered-gliding/climbing flight can be seen as an extension of flap-gliding flight, which is a flight mode known in the research on animal flight. This paper shows that the powered glide enables a decrease in aerodynamic drag, as well as an explanation of the underlying physical mechanism. I also developed a flight mechanics model of powered/gliding climbing flight in bats. Results based on this model show that fuel consumption can be reduced. Thus, a substantial fuel saving can be achieved when compared with the best flight at constant altitude, which is classically considered as the flight mode requiring the lowest fuel consumption.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"14 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SOX2 and NR2F1 coordinate the gene expression program of the early postnatal visual thalamus. SOX2和NR2F1协调出生后早期视丘脑的基因表达程序。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 Epub Date: 2025-08-01 DOI: 10.1242/bio.062014

Linda Serra, Anna Nordin, Mattias Jonasson, Carolina Marenco, Guido Rovelli, Annika Diebels, Francesca Gullo, Sergio Ottolenghi, Federico Zambelli, Michèle Studer, Giulio Pavesi, Claudio Cantù, Silvia K Nicolis, Sara Mercurio

{"title":"SOX2 and NR2F1 coordinate the gene expression program of the early postnatal visual thalamus.","authors":"Linda Serra, Anna Nordin, Mattias Jonasson, Carolina Marenco, Guido Rovelli, Annika Diebels, Francesca Gullo, Sergio Ottolenghi, Federico Zambelli, Michèle Studer, Giulio Pavesi, Claudio Cantù, Silvia K Nicolis, Sara Mercurio","doi":"10.1242/bio.062014","DOIUrl":"10.1242/bio.062014","url":null,"abstract":"The thalamic dorsolateral geniculate nucleus (dLGN) receives visual input from the retina via the optic nerve, and projects to the cortical visual area, where eye-derived signals are elaborated. The transcription factors SOX2 and NR2F1 are directly involved in the differentiation of dLGN neurons, based on mouse work and patient mutations leading to vision defects. However, whether they regulate each other, or control common targets is still unclear. By RNA-seq analysis of neonatal dLGN from thalamo-specific Sox2 and Nr2f1 mouse mutants, we found a striking overlap of deregulated genes. Among them, Vgf, encoding a cytokine transported along thalamic-cortical axons is strongly downregulated in both mutants. Direct SOX2 binding to some of these genes was confirmed by CUT&RUN, which identified a SOX2 chromatin-binding pattern characteristic of the dLGN. Collectively, our genetic and molecular analyses on the SOX2 and NR2F1-coregulated genes contribute to our understanding of the gene regulatory network driving the differentiation and connectivity of thalamic neurons, and the vision impairments caused by mutations in these genes.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12352279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiomics analysis of GSTP1 knockdown pancreatic cancer cells reveals key regulators of redox and metabolic homeostasis. GSTP1敲低胰腺癌细胞的多组学分析揭示了氧化还原和代谢稳态的关键调节因子。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 Epub Date: 2025-08-14 DOI: 10.1242/bio.061986

Jenna N Duttenhefner, Rahul R Singh, Katherine Schmidt, Katie M Reindl

{"title":"Multiomics analysis of GSTP1 knockdown pancreatic cancer cells reveals key regulators of redox and metabolic homeostasis.","authors":"Jenna N Duttenhefner, Rahul R Singh, Katherine Schmidt, Katie M Reindl","doi":"10.1242/bio.061986","DOIUrl":"10.1242/bio.061986","url":null,"abstract":"Glutathione S transferase pi-1 (GSTP1) is a detoxification enzyme essential for oxidative homeostasis. In cancer, GSTP1 has been implicated in tumorigenicity, cell cycle progression, and chemoresistance. While GSTP1 depletion has been associated with decreased cancer growth in various models, the mechanism remains poorly understood. This study investigates GSTP1 as a therapeutic target for pancreatic ductal adenocarcinoma (PDAC) using inducible knockdown models. We demonstrate that GSTP1 loss disrupts redox balance, impairs cell survival, and induces metabolic adaptations. Multiomics analysis characterized the global impact of inducible GSTP1 knockdown on the transcriptome and proteome of PDAC cells, identifying 550 differentially expressed genes and 62 proteins. Notably, 43 of these showed consistent regulation at both the mRNA and protein levels. We identify dysregulation of key stress response proteins, including dimethylarginine dimethylaminohydrolase 1 (DDAH1), involved in nitric oxide metabolism, and protein disulfide isomerase A6 (PDIA6), which maintains protein homeostasis. The interplay between GSTP1, DDAH1 and PDIA6 highlights the complexity of redox regulation in pancreatic cancer and suggests that targeting GSTP1 may offer a new therapeutic approach for PDAC.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pro-angiogenic and hypoxic zebrafish model as a novel platform for anti-angiogenic drug testing. 促血管生成和缺氧斑马鱼模型作为抗血管生成药物测试的新平台。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 Epub Date: 2025-08-11 DOI: 10.1242/bio.061863

Vinoth S, Kirankumar Santhakumar

{"title":"A pro-angiogenic and hypoxic zebrafish model as a novel platform for anti-angiogenic drug testing.","authors":"Vinoth S, Kirankumar Santhakumar","doi":"10.1242/bio.061863","DOIUrl":"10.1242/bio.061863","url":null,"abstract":"Zebrafish is a valuable model for antiangiogenic drug testing. We hypothesized that the efficacy of antiangiogenic compounds might vary in hypoxic tissue environments compared to normal tissue. To explore this, we established a chemically induced zebrafish model using DMOG, which inhibits prolyl hydroxylases, and a genetic model by knocking out vhl gene via CRISPR/Cas9 to activate hypoxia signaling. In wild-type larvae, the antiangiogenic drug sorafenib inhibited blood vessel growth. However, in the DMOG model and vhl-/- model, no inhibition occurred in sub-intestinal vessel (SIV) upon sorafenib treatment. Also, gene expression analysis showed that the DMOG induced hypoxia had 20-fold increase in phd3 expression, a marker for hypoxia signaling activation, which rose to 65-fold and 280-fold with sorafenib treatment at the concentration 0.1 μM and 0.2 μM, respectively. In the vhl-/- model phd3 expression was found to be increased to 220-fold and reaching up to 400-fold with sorafenib treatment. This increased activation of hypoxia signaling elevated the proangiogenic factors like vegfaa, vegfab and vegfd, which might have protected the SIV region from sorafenib treatment in hypoxic models. This confirms that the hypoxia zebrafish models gained resistance against chemotherapeutic drugs by increasing the cellular hypoxia levels. Thus, our zebrafish model for hypoxia provides evidence that the efficacy of chemotherapy for cancer significantly depends on hypoxic microenvironment.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"14 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of lmx1b paralogues in zebrafish reveals divergent roles in skeletal, kidney and muscle development. 斑马鱼中lmx1b同源物的特征揭示了在骨骼、肾脏和肌肉发育中的不同作用。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 Epub Date: 2025-08-19 DOI: 10.1242/bio.062038

Joanna J Moss, Chris R Neal, Erika Kague, Jon D Lane, Chrissy L Hammond

{"title":"Characterisation of lmx1b paralogues in zebrafish reveals divergent roles in skeletal, kidney and muscle development.","authors":"Joanna J Moss, Chris R Neal, Erika Kague, Jon D Lane, Chrissy L Hammond","doi":"10.1242/bio.062038","DOIUrl":"10.1242/bio.062038","url":null,"abstract":"LMX1B, a LIM-homeodomain family transcription factor, plays critical roles in the development of multiple tissues, including limbs, eyes, kidneys, brain, and spinal cord. Mutations in the human LMX1B gene cause the rare autosomal-dominant disorder Nail-patella syndrome, which affects development of limbs, eyes, brain, and kidneys. In zebrafish, lmx1b has two paralogues: lmx1ba and lmx1bb. While lmx1b morpholino data exists, stable mutants were previously lacking. Here, we describe the characterisation of lmx1b stable mutant lines, with a focus on development of tissues that are affected in Nail-patella syndrome. We demonstrate that the lmx1b paralogues have divergent developmental roles in zebrafish, with lmx1ba affecting skeletal and neuronal development, and lmx1bb affecting renal development. The double mutant, representing loss of both paralogues (lmx1b dKO) showed a stronger phenotype, which included additional defects to trunk muscle patterning, and a failure to fully inflate the notochord leading to a dramatic reduction in body length. Overall, these mutant lines demonstrate the utility of zebrafish for modelling Nail-patella syndrome and describe a previously undescribed role for lmx1b in notochord cell inflation.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"14 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanosensitive localization of Diversin highlights its function in vertebrate morphogenesis and planar cell polarity. Diversin的机械敏感性定位突出了其在脊椎动物形态发生和平面细胞极性中的作用。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 Epub Date: 2025-08-11 DOI: 10.1242/bio.062128

Satheeja Santhi Velayudhan, Chih-Wen Chu, Keiji Itoh, Sergei Y Sokol

{"title":"Mechanosensitive localization of Diversin highlights its function in vertebrate morphogenesis and planar cell polarity.","authors":"Satheeja Santhi Velayudhan, Chih-Wen Chu, Keiji Itoh, Sergei Y Sokol","doi":"10.1242/bio.062128","DOIUrl":"10.1242/bio.062128","url":null,"abstract":"Diversin is a vertebrate homolog of the core planar cell polarity (PCP) protein Diego. Here we studied the function of Diversin in Xenopus embryo morphogenesis and its subcellular localization at different locations in superficial ectoderm cells. Depletion of Diversin in the neuroectoderm inhibited apical domain size and neural tube closure and disrupted the polarized localization of endogenous Vangl2, another PCP protein. Whereas Diversin puncta were randomly distributed in early ectoderm, they acquired planar polarity in the neuroectoderm in a stage- and position-specific manner. We find that Diversin is accumulated at the cell junctions adjacent to apically constricting cells at the Xenopus neural plate border and the gastrula blastopore lip. Moreover, Diversin cytoplasmic puncta redistributed in the direction of the pulling forces from the cells with constricting apical domains, suggesting a mechanosensitive process. PCP complexes of Dishevelled (Dvl2) and Diversin or the mechanosensitive adaptor ADIP exhibited planar polarity in the neural plate and the wound edge and promoted wound healing. We propose that Diversin- and Dvl2-containing PCP complexes control morphogenesis in a tension-dependent manner.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of rising temperatures on the bacterial communities of Aphaenogaster ants. 温度升高对隐腹蚁细菌群落的影响。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 DOI: 10.1242/bio.062145

Lily A Kelleher, Manuela O Ramalho

{"title":"Impact of rising temperatures on the bacterial communities of Aphaenogaster ants.","authors":"Lily A Kelleher, Manuela O Ramalho","doi":"10.1242/bio.062145","DOIUrl":"10.1242/bio.062145","url":null,"abstract":"Studies have shown that biodiversity will be impacted by global climate change, with the effect on ants just beginning to be documented. The influence on ant symbiotic bacterial communities remains understudied. Aphaenogaster Mayr, 1853, are seed-dispersing ants in deciduous forests and their bacterial communities have just been uncovered; however, much is unknown. We aim to determine the impact that warming temperatures will have on Aphaenogaster survival and on their bacterial communities. Ants from four colonies were collected from West Chester, PA, USA and entire colonies were subjected to a control temperature (22°C). After 6-12 months, the same colonies were subjected to an experimental temperature (32°C). DNA was then extracted from ants of all development stages and the 16S rRNA gene was amplified and sequenced following the NGS amplicon approach. The findings revealed that Aphaenogaster ant mortality rates increased, and their symbiotic bacterial communities changed in warmer temperatures. This resulted in a decrease in the presence of Wolbachia spp. and an increase in the presence of Corynebacterium sp. This study reveals important information about the impact of warming temperature on Aphaenogaster ants, and we suggest methods to help protect these ants and other insects in the future.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent protein tags for human tropomyosin isoform comparison. 人原肌球蛋白异构体比较的荧光蛋白标记。

IF 1.7 4区生物学

Biology Open Pub Date : 2025-08-15 Epub Date: 2025-07-29 DOI: 10.1242/bio.061992

Will Scott, Vitaliia Polutranko, Jakub Milczarek, Ian Hands-Portman, Mohan K Balasubramanian

{"title":"Fluorescent protein tags for human tropomyosin isoform comparison.","authors":"Will Scott, Vitaliia Polutranko, Jakub Milczarek, Ian Hands-Portman, Mohan K Balasubramanian","doi":"10.1242/bio.061992","DOIUrl":"10.1242/bio.061992","url":null,"abstract":"Tropomyosin is an important actin cytoskeletal protein underpinning processes such as muscle contraction, cell shape and cell division. Defects in tropomyosin function can lead to diseases, including some myopathies and allergies. In cells, tropomyosin molecules form coiled-coil dimers, which then polymerise end-to-end with other dimers for actin association. Tropomyosin is challenging to tag for in vivo fluorescence microscopy without perturbing its polymerisation interfaces. We recently developed a fluorescent tag comprising a 40-amino acid flexible linker capable of detecting tropomyosin in S. pombe actin cables and the actomyosin ring, and in patch-like structures that were previously unappreciated. We also used this strategy successfully to tag human TPM2.2, a prominent human muscle isoform. Here, we expanded this tool to visualise eight other human tropomyosin isoforms, using mNeonGreen, mCherry, mStayGold(E138D) and mScarlet3-H tags. All showed typical tropomyosin fluorescence, no signs of cytotoxicity and are compatible with super-resolution microscopy. These tools singly or in combination should aid detailed mechanistic investigations of tropomyosin isoforms.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12352278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0