Biology Open最新文献_第4页

GSK3 coordinately regulates mitochondrial activity and nucleotide metabolism in quiescent oocytes. GSK3协调调节静止卵母细胞的线粒体活性和核苷酸代谢。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-24 DOI: 10.1242/bio.061815

Leah Eller, Lei Wang, Mehmet Oguz Gok, Helin Hocaoglu, Shenlu Qin, Parul Gupta, Matthew H Sieber

{"title":"GSK3 coordinately regulates mitochondrial activity and nucleotide metabolism in quiescent oocytes.","authors":"Leah Eller, Lei Wang, Mehmet Oguz Gok, Helin Hocaoglu, Shenlu Qin, Parul Gupta, Matthew H Sieber","doi":"10.1242/bio.061815","DOIUrl":"10.1242/bio.061815","url":null,"abstract":"As cells transition between periods of growth and quiescence, their metabolic demands change. During this transition, cells must coordinate changes in mitochondrial function with the induction of biosynthetic processes. Mitochondrial metabolism and nucleotide biosynthesis are key rate-limiting factors in regulating early growth. However, it remains unclear what coordinates these mechanisms in developmental systems. Here, we show that during quiescence, as mitochondrial activity drops, nucleotide breakdown increases. However, at fertilization, mitochondrial oxidative metabolism and nucleotide biosynthesis are coordinately activated to support early embryogenesis. We have found that the serine/threonine kinase GSK3 is a key factor in coordinating mitochondrial metabolism with nucleotide biosynthesis during transitions between quiescence and growth. Silencing GSK3 in quiescent oocytes causes increased levels of mitochondrial activity and a shift in the levels of several redox metabolites. Interestingly, silencing GSK3 in quiescent oocytes also leads to a precocious induction of nucleotide biosynthesis in quiescent oocytes. Taken together, these data indicate that GSK3 functions to suppress mitochondrial oxidative metabolism and prevent the premature onset of nucleotide biosynthesis in quiescent eggs. These data reveal a key mechanism that coordinates mitochondrial function and nucleotide synthesis with fertilization.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of silver and gold nanoparticles green synthesis by Artemisia annua hairy root extracts. 黄花蒿毛根提取物绿色合成银纳米粒子和金纳米粒子的比较。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-19 DOI: 10.1242/bio.061739

Taisa Bohdanovych, Pavlo Kuzema, Viktor Anishchenko, Volodymyr Duplij, Maksym Kharchuk, Viktoriia Lyzhniuk, Anatolij Shakhovsky, Nadiia Matvieieva

{"title":"Comparison of silver and gold nanoparticles green synthesis by Artemisia annua hairy root extracts.","authors":"Taisa Bohdanovych, Pavlo Kuzema, Viktor Anishchenko, Volodymyr Duplij, Maksym Kharchuk, Viktoriia Lyzhniuk, Anatolij Shakhovsky, Nadiia Matvieieva","doi":"10.1242/bio.061739","DOIUrl":"10.1242/bio.061739","url":null,"abstract":"The green synthesis of metal nanoparticles (NPs) has garnered significant attention due to its simplicity, cost-effectiveness, and environmental sustainability. Gold NPs (AuNPs) and silver NPs (AgNPs) are widely employed across various industries, agriculture, and medicine owing to their unique physicochemical properties. This study explores the feasibility of synthesizing metal NPs through green methods using ethanolic (70%) extracts from Artemisia annua hairy roots. These extracts were found to contain reducing agents, primarily phenolic compounds, as identified by HPLC and MALDI-MS analyses. The phenolic compounds included hydroxybenzoic acids (e.g. p-coumaric and gallic acids) and hydroxycinnamic acids (e.g. caffeic acid and its derivatives such as chlorogenic, dicaffeoylquinic, and rosmarinic acids). The synthesis and structural characteristics of AuNPs and AgNPs were systematically compared. AgNPs formed a stable colloidal solution over extended periods, while AuNPs exhibited instability due to significant NP aggregation and precipitation. Furthermore, the photocatalytic activities of these NPs in the degradation of Methylene Blue were evaluated. AuNPs demonstrated substantial photocatalytic activity, whereas AgNPs exhibited negligible catalytic effects. This study highlights the potential and limitations of A. annua hairy root extracts in the biosynthesis of AuNPs and AgNPs, providing insights into their structural and functional differences.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Science under siege: protecting scientific progress in turbulent times. 围攻中的科学：在动荡时期保护科学进步。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-13 DOI: 10.1242/bio.061972

James Briscoe, Craig E Franklin, Daniel A Gorelick, E Elizabeth Patton, Michael Way

引用次数: 0

Alveolar epithelial paxillin in postnatal lung alveolar development. 肺泡上皮 paxillin 在出生后肺泡发育过程中的作用

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-20 DOI: 10.1242/bio.061939

Mikaela Scheer, Priscilla Kyi, Tadanori Mammoto, Akiko Mammoto

引用次数: 0

Early-life environment shapes claw bilateral asymmetry in the European lobster (Homarus gammarus). 早期生活环境塑造了欧洲龙虾（Homarus gammarus）的双爪不对称。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-20 DOI: 10.1242/bio.061901

Lorenzo Latini, Gioia Burini, Valeria Mazza, Giacomo Grignani, Riccardo De Donno, Eleonora Bello, Elena Tricarico, Stefano Malavasi, Giuseppe Nascetti, Daniele Canestrelli, Claudio Carere

{"title":"Early-life environment shapes claw bilateral asymmetry in the European lobster (Homarus gammarus).","authors":"Lorenzo Latini, Gioia Burini, Valeria Mazza, Giacomo Grignani, Riccardo De Donno, Eleonora Bello, Elena Tricarico, Stefano Malavasi, Giuseppe Nascetti, Daniele Canestrelli, Claudio Carere","doi":"10.1242/bio.061901","DOIUrl":"10.1242/bio.061901","url":null,"abstract":"Developmental plasticity refers to an organism's ability to adjust its development in response to changing environmental conditions, leading to changes in behaviour, physiology, or morphology. This adaptability is crucial for survival and helps organisms to cope with environmental challenges throughout their lives. Understanding the mechanisms underlying developmental plasticity, particularly how environmental and ontogenetic factors shape functional traits, is fundamental for both evolutionary biology and conservation efforts. In this study we investigated the effects of early-life environmental conditions on the development of claw asymmetry in juvenile European lobsters (Homarus gammarus, N=244), a functional trait essential for survival and ecological success. Juveniles were randomly divided between four different rearing conditions characterized by the presence or absence of physical enrichments (e.g. substrate and shelters), which were introduced at different developmental stages in separated groups to assess the timing and nature of their effect. Results revealed that exposure to substrate alone, without additional stimuli, consistently promoted claw asymmetry, regardless of the timing of its introduction, while the 6th developmental stage emerged as the critical period for claw differentiation. By identifying the environmental factors that influence developmental outcomes in lobsters, and the timing of these effects, this study improves our understanding of developmental plasticity and offers valuable insights for optimizing conservation aquaculture and reintroduction strategies.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primed bovine embryonic stem cell lines can be derived at diverse stages of blastocyst development with similar efficiency and molecular characteristics. 引物牛胚胎干细胞系可以在囊胚发育的不同阶段获得，具有相似的效率和分子特征。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-07 DOI: 10.1242/bio.061819

Carly Guiltinan, Ramon C Botigelli, Juliana I Candelaria, Justin M Smith, Rachel B Arcanjo, Anna C Denicol

{"title":"Primed bovine embryonic stem cell lines can be derived at diverse stages of blastocyst development with similar efficiency and molecular characteristics.","authors":"Carly Guiltinan, Ramon C Botigelli, Juliana I Candelaria, Justin M Smith, Rachel B Arcanjo, Anna C Denicol","doi":"10.1242/bio.061819","DOIUrl":"10.1242/bio.061819","url":null,"abstract":"In this study, we established bovine embryonic stem cell (bESC) lines from early (eBL) and full (BL) blastocysts to determine the efficiency of bESC derivation from an earlier embryonic stage and compare the characteristics of the resulting lines. Using established medium and protocols for derivation of primed bESCs from expanded blastocysts, we derived bESC lines from eBLs and BLs with the same efficiency (4/12 each, 33%). Regardless of original blastocyst stage, bESC lines had a similar phenotype, including differentiation capacity, stable karyotype, and pluripotency marker expression over feeder-free transition and long-term culture. Transcriptome and functional analyses indicated that eBL- and BL-derived lines were in primed pluripotency. We additionally compared RNA-sequencing data from our lines to bovine embryos and stem cells from other recent reports, finding that base medium was the predominant source of variation among cell lines. In conclusion, our results show that indistinguishable bESC lines can be readily derived from eBL and BL, widening the pool of embryos available for bESC establishment. Finally, our investigation points to sources of variation in cell phenotype among recently reported bESC conditions, opening the door to future studies investigating the impact of factors aside from signaling molecules on ESC derivation, maintenance, and performance.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast & Fair peer review: a bold experiment in scientific publishing. 快速公平的同行评议：科学出版领域的一次大胆实验。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-25 DOI: 10.1242/bio.061982

Daniel A Gorelick, Alejandra Clark

引用次数: 0

From fossils to future - Jane Francis on polar exploration and changing climates. 从化石到未来——简·弗朗西斯谈极地探险和气候变化。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-24 DOI: 10.1242/bio.061973

Katie Pickup

引用次数: 0

Deciphering the flapping frequency allometry: unveiling the role of sustained body attitude in the aerodynamic scaling of normal hovering animals. 解析扑动频率异速：揭示持续身体姿态在正常悬停动物气动尺度中的作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-14 DOI: 10.1242/bio.061932

Jeremy Pohly, Chang-Kwon Kang, Hikaru Aono

引用次数: 0

Neurogenetic disorders associated with mutations in the FERRY complex: a novel disease class? 与FERRY复合物突变相关的神经遗传疾病：一种新的疾病类别？

IF 1.8 4区生物学

Biology Open Pub Date : 2025-03-15 Epub Date: 2025-03-10 DOI: 10.1242/bio.061808

R Madison Riffe, Gerald B Downes

{"title":"Neurogenetic disorders associated with mutations in the FERRY complex: a novel disease class?","authors":"R Madison Riffe, Gerald B Downes","doi":"10.1242/bio.061808","DOIUrl":"10.1242/bio.061808","url":null,"abstract":"The five-subunit endosomal Rab5 and RNA/ribose intermediary (FERRY) complex is a newly described protein complex consisting of TBCK, PPP1R21, FERRY3 (previously C12orf4), CRYZL1, and GATD1. The FERRY complex is proposed to function as a Rab5 effector to shuttle mRNA to the cell periphery for local translation, a process especially important in cells with far reaching processes. Interestingly, three members of the FERRY complex are associated with ultra-rare neurogenetic disorders. Mutation of TBCK causes TBCK syndrome, mutation of PPP1R21 is associated with PPP1R21-related intellectual disability, and mutation of FERRY3 results in an autosomal recessive intellectual disability. Neurologic disorders have yet to be associated with mutation of GATD1 or CRYZL1. Here, we provide a review of each FERRY complex-related neurologic disorder and draw clinical comparisons between the disease states. We also discuss data from the current cellular and animal models available to study these disorders, which is notably disparate and scattered across different cell types and systems. Taken together, we explore the possibility that these three diseases may represent one shared disease class, which could be further understood by combining and comparing known information about each individual disease. If true, this could have substantial implications on our understanding of the cellular role of the FERRY complex and on treatment strategies for affected individuals, allowing researchers, clinicians, and patient organizations to maximize the utility of research efforts and resources to support patients with these disorders.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"14 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0