Biology Open最新文献_第8页

Allometric scaling and social modulation of resting metabolic rate in a eusocial mammal (Heterocephalus glaber). 一种真社会性哺乳动物（异速缩放和静息代谢率的社会调节）。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-24 DOI: 10.1242/bio.062586

Jack E Thirkell, Chris G Faulkes, Hana N Merchant, James D Gilbert, Nigel C Bennett, Daniel W Hart, Monica A Daley, Steven J Portugal

{"title":"Allometric scaling and social modulation of resting metabolic rate in a eusocial mammal (Heterocephalus glaber).","authors":"Jack E Thirkell, Chris G Faulkes, Hana N Merchant, James D Gilbert, Nigel C Bennett, Daniel W Hart, Monica A Daley, Steven J Portugal","doi":"10.1242/bio.062586","DOIUrl":"10.1242/bio.062586","url":null,"abstract":"Resting metabolic rate (RMR) underpins energy allocation to survival, reproduction, and longevity, yet its drivers in highly structured social systems remain unclear. We measured RMR in 76 naked mole-rats (Heterocephalus glaber) from five colonies to test effects of body mass, sex, reproductive status, age, social rank, and colony stability in this eusocial mammal. Body mass was the strongest predictor of RMR. Nevertheless, absolute RMR values were far below those predicted for mammals of similar mass, confirming metabolic suppression in this strictly subterranean species. Social factors produced subtler patterns. Among non-breeders, males had slightly higher (but not significantly so) RMR than females despite similar body mass. Breeding females showed elevated RMR relative to non-breeding females, but not significantly so. Age and dominance rank did not influence RMR once body mass was controlled. Colony identity explained little variation overall, although one colony showed altered metabolic scaling after social conflict, suggesting colony-level metabolic plasticity associated with hierarchical instability.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13382701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the impact of poly(beta amino) ester-mediated FOXJ1 mRNA delivery on differentiation of primary human bronchial epithelial cells. 探讨聚氨基酯介导的FOXJ1 mRNA传递对人支气管上皮细胞分化的影响。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-12 DOI: 10.1242/bio.062419

Rafaela Konstantinidi, Mira Subramanian, Laura Yates, Clare M Lloyd, Sejal Saglani, Asha K Patel

{"title":"Investigating the impact of poly(beta amino) ester-mediated FOXJ1 mRNA delivery on differentiation of primary human bronchial epithelial cells.","authors":"Rafaela Konstantinidi, Mira Subramanian, Laura Yates, Clare M Lloyd, Sejal Saglani, Asha K Patel","doi":"10.1242/bio.062419","DOIUrl":"10.1242/bio.062419","url":null,"abstract":"Primary human basal bronchial epithelial cells (HBECs) are an important population of progenitor cells capable of self-renewal and differentiation to maintain airway homeostasis. In air-liquid interface (ALI) culture, HBECs undergo mucociliary differentiation, providing a robust physiologic model to evaluate novel therapeutics such as in vitro-transcribed messenger RNA (IVT-mRNA). However, the impact of IVT-mRNA delivery on the differentiation potential of basal HBECs remains poorly characterised. Poly(beta amino) ester (PBAE) nanoparticles have demonstrated effective airway delivery of IVT-mRNA in various preclinical studies. Here, we aimed to understand the impact of PBAE-mediated mRNA transfection on basal HBEC differentiation at the ALI. We investigated IVT-mRNA encoding the ciliogenesis transcription factor Forkhead box J1 (FOXJ1) as a model tool for transient overexpression in primary basal HBECs and characterised its subsequent impact on epithelial integrity and differentiation at the ALI. PBAE-mediated delivery of FOXJ1 mRNA to submerged primary HBECs resulted in approximately 50% FOXJ1-positive cells and transient upregulation of key ciliogenesis-related genes, including DNALI1 and RSPH9. Following 28 days of differentiation at the ALI, FOXJ1- or reporter mRNA-transfected cultures displayed normal epithelial morphology, with tight junction and differentiation markers, proportions of secretory and ciliated cells, and ciliary ultrastructure comparable to those of non-transfected controls. These data indicate that PBAE-mediated IVT-mRNA delivery can transiently increase encoded protein expression in basal primary HBECs without impeding mucociliary differentiation.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"15 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13312916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148223205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential response variability of black-capped chickadees to wingbeat sounds and vocalizations. 黑冠山雀对振翅声和发声的差异反应变异性。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-24 DOI: 10.1242/bio.062598

Prateek K Sahu, Kailyn Nottebrock, Julian Ratch, Sarah M L Smeltz, Katharine H Stenstrom, Moriah J Deimeke, Christopher B Sturdy

{"title":"Differential response variability of black-capped chickadees to wingbeat sounds and vocalizations.","authors":"Prateek K Sahu, Kailyn Nottebrock, Julian Ratch, Sarah M L Smeltz, Katharine H Stenstrom, Moriah J Deimeke, Christopher B Sturdy","doi":"10.1242/bio.062598","DOIUrl":"10.1242/bio.062598","url":null,"abstract":"Prey species use various cues (visual, acoustic, olfactory) to detect approaching predators. Among these, visual cues are generally considered reliable, providing immediate and accurate information about potential dangers. While acoustic cues also play a key role in threat assessment in birds, the relative importance of different auditory cues and their integration are less well studied. Here, we investigated how black-capped chickadees (Poecile atricapillus) respond to predator and non-predator wingbeats and vocalizations presented independently and in sequence with each other. In an exploratory playback experiment with 16 chickadees, we found no differences in how chickadees responded to predator versus non-predator acoustic cues when presented individually or sequentially. However, sequential presentations of acoustic cues had reduced response variance compared to solo playbacks. These findings suggest that black-capped chickadees may lack specific recognition mechanisms for discriminating predator from non-predator wingbeats in isolation but may integrate multiple cues to improve threat assessment reliability. Our results demonstrate the potential role of non-vocal cues in acoustic threat assessment in small passerines and the importance of considering within-modality cue integration in anti-predator behaviour studies. Future behavioural study designs would benefit from incorporating the current findings.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13382834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148216610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative stress: a sex-specific cost of parental care. 氧化应激：亲代抚育的性别特异性成本。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-24 DOI: 10.1242/bio.062571

Norma Martínez-Lendech, Ivette Marai Villa-Villaseñor, Luis Mendoza-Cuenca, Jorge Contreras-Garduño

{"title":"Oxidative stress: a sex-specific cost of parental care.","authors":"Norma Martínez-Lendech, Ivette Marai Villa-Villaseñor, Luis Mendoza-Cuenca, Jorge Contreras-Garduño","doi":"10.1242/bio.062571","DOIUrl":"10.1242/bio.062571","url":null,"abstract":"Parental care involves post-zygotic behaviors that promote offspring survival but can diminish parental survival and future reproductive potential. Although care is typically shared, males and females often differ in the nature and intensity of care provided. In the wild, we characterized pair formation and parental care (nesting and fry care) in Herichthys bartoni. Behavioral investment was quantified as the proportion of observation time allocated to each activity. Fin tissue samples were collected to evaluate oxidative stress across reproductive stages. We observed stage- and sex-specific behavioral differences. During pair formation, both sexes fed and engaged in synchronized swimming, with no significant sex differences. During nesting, females allocated a significantly greater proportion of observation time to nest cleaning and egg aeration, whereas no sex differences were found during fry care. Males were larger and exhibited higher total antioxidant levels, independent of body size. Antioxidant levels peaked during pair formation and fry care, with catalase activity being higher in females during fry care. No sex differences were detected in superoxide dismutase, hydrogen peroxide, or nitric oxide. These findings suggest that biparental care in H. bartoni involves distinct behavioral roles and physiological costs, with females bearing greater oxidative demands linked to their increased direct investment in offspring care.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13382992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation-induced cytidine deaminase promotes DNA demethylation and expression of the Ninjurin-2 gene. 激活诱导胞苷脱氨酶促进DNA去甲基化和Ninjurin-2基因的表达。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-12 DOI: 10.1242/bio.062001

Toshiyuki Okada, Yusuke Toyoda, Shigeaki Saitoh

{"title":"Activation-induced cytidine deaminase promotes DNA demethylation and expression of the Ninjurin-2 gene.","authors":"Toshiyuki Okada, Yusuke Toyoda, Shigeaki Saitoh","doi":"10.1242/bio.062001","DOIUrl":"10.1242/bio.062001","url":null,"abstract":"Activation-induced cytidine deaminase (AID) is expressed in germinal center B cells and contributes to somatic hypermutation and isotype switching of immunoglobulins. AID is also reportedly expressed in colonic epithelial cells (CECs) during inflammation. However, the physiological roles of AID in CECs are largely unknown. Here, we identify 619 genes, the expression of which is induced in a colorectal cancer (CRC) cell line (DLD-1) by overexpression of AID. These genes include those associated with the phosphoinositide-3-kinase-protein kinase B, mammalian target of rapamycin and mitogen-activated protein kinase signaling pathways. We focused on the NINJ2 gene, the expression of which increased more than fivefold in AID-overexpressing DLD-1 cells and decreased in colitis-induced CECs of AID-knockout mice, compared with those of wild-type mice. We demonstrate that AID binds to the promoter/enhancer region of NINJ2 and induces DNA demethylation, which is often accompanied by transcriptional upregulation. Additionally, the recovery from induced colitis was significantly delayed in AID-knockout mice. These findings collectively suggest that inflammation-increased AID upregulation in CECs enhances NINJ2 transcription through DNA demethylation, potentially facilitating wound healing during recovery.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"15 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13312926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148223182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fructose impairs brown adipogenesis by promoting thyroid hormone resistance in differentiated brown adipocytes. 果糖通过促进分化的棕色脂肪细胞对甲状腺激素的抵抗来损害棕色脂肪的形成。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-22 DOI: 10.1242/bio.062648

Xiangdong Wu, Salaheldeen Elsaid, Lei Zhang, Eric Y Chan, Junkai Hu, Liqing Yu, Sui Seng Tee

{"title":"Fructose impairs brown adipogenesis by promoting thyroid hormone resistance in differentiated brown adipocytes.","authors":"Xiangdong Wu, Salaheldeen Elsaid, Lei Zhang, Eric Y Chan, Junkai Hu, Liqing Yu, Sui Seng Tee","doi":"10.1242/bio.062648","DOIUrl":"10.1242/bio.062648","url":null,"abstract":"Excessive fructose consumption is increasingly linked to obesity, diabetes, non-alcoholic fatty liver disease (NAFLD), and cardiovascular disease, yet its impact on brown adipocytes remains poorly defined. Here, we investigated how fructose alters brown adipogenesis with a focus on thyroid hormone receptor (THR) signaling. Differentiation of mouse brown preadipocytes in fructose-containing medium resulted in markedly reduced lipid droplet size and number, impaired expression of adipogenic markers (PPARγ, FABP4), and a loss of the brown adipocyte identity protein UCP-1. Whereas triiodothyronine (T3) and the THRβ-selective agonist resmetirom dose-dependently enhanced UCP-1 expression in glucose medium, neither T3 nor resmetirom restored UCP-1 in fructose medium, revealing thyroid hormone resistance. Mechanistically, fructose specifically downregulated THR but not its heterodimer partner, retinoid X receptor (RXR), via ubiquitin-mediated degradation. Overexpression of THR rescued UCP-1 in glucose medium and promoted dynamic lipid turnover but failed to restore UCP-1 or lipid remodeling in fructose medium, indicating a defect in THR transcriptional regulation. Our secretome proteomics revealed that both the sugar source and THR overexpression strongly remodel the secreted protein landscape of brown adipocytes. The data indicate that fructose both reconfigures metabolic and signaling networks and blunts oxidative resilience, while excess THR activity in fructose conditions disrupts energy metabolism and increases the risk of protein misfolding. All these could contribute to THR dysfunction and impair adipogenesis. Collectively, these findings identify fructose as a potent inhibitor of brown adipogenesis, suppressing THR-dependent UCP-1 expression by inducing thyroid hormone resistance through a defect in THR-mediated transcriptional regulation. This mechanism links dietary fructose to impaired brown adipocytes and metabolic imbalance.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13382702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148155219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives from the 2024 School on Neuropharmacology Research and Drug Development in Harare, Zimbabwe: insights from participants. 来自津巴布韦哈拉雷2024年神经药理学研究和药物开发学院的观点：来自参与者的见解。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-10 DOI: 10.1242/bio.062217

John Senanu, Bertrand Yuwong Wanyu, Jessica Burns, Victor Bassey Archibong, Rosa M Chinheya, Nji S Ombel Musa, Shingirayi Zengeni, Asmaa Haj-Khlifa, Kehinde Oluwaseyi Adeniji, Rhoda Mama Kolo, Mooki Caroline, Fatma Zahra Sakhri, Elias Chipofya

{"title":"Perspectives from the 2024 School on Neuropharmacology Research and Drug Development in Harare, Zimbabwe: insights from participants.","authors":"John Senanu, Bertrand Yuwong Wanyu, Jessica Burns, Victor Bassey Archibong, Rosa M Chinheya, Nji S Ombel Musa, Shingirayi Zengeni, Asmaa Haj-Khlifa, Kehinde Oluwaseyi Adeniji, Rhoda Mama Kolo, Mooki Caroline, Fatma Zahra Sakhri, Elias Chipofya","doi":"10.1242/bio.062217","DOIUrl":"10.1242/bio.062217","url":null,"abstract":"Sub-Saharan Africa is experiencing a rising burden of neurological and mental disorders. The International Brain Research Organization (IBRO)-funded 2nd School on Neuropharmacology Research and Drug Development (NRDD) was held in Harare, Zimbabwe, 2024. The meeting aimed to (1) provide a theoretical foundation in neuropharmacology; (2) develop skills in experimental design, pharmacokinetics, and drug evaluation; (3) foster interdisciplinary collaboration and networking; and (4) promote cultural and scientific exchange. Thirteen participants from eight African countries attended the 1-week program. This Meeting Review evaluates the extent to which the meeting achieved its goals, drawing on structured reflections from all participants, and describes the scientific, networking, cultural, and logistical dimensions of the experience. The school successfully equipped participants with foundational knowledge in neuropharmacology and drug development. Future editions would benefit from extended hands-on sessions and structured post-program follow-up.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":"15 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13382971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148216563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Assessment of seasonal, substrate and hormonal factors influencing the propagation and rooting of Camellia rubriflora's cuttings. 回顾：季节、基质和激素因素对红山茶扦插繁殖和生根的影响。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-23 DOI: 10.1242/bio.062662

Tinh Thi Nguyen, Vinh Thi Pham, Huong Thi Nguyen, Hoai Thi Duong, Cuong Manh Duong

引用次数: 0

Glimpse into the role of Kupffer cells in a spheroid model of metabolic dysfunction-associated steatohepatitis (MASH). Kupffer细胞在代谢功能障碍相关脂肪性肝炎（MASH）球形模型中的作用。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 Epub Date: 2026-06-03 DOI: 10.1242/bio.062552

Niklas Bogovic, Maria Drießlein, Marie Keller, Hans J Schlitt, Edward K Geissler, Lydia Schneider, Philipp Kreiner, Henrik Junger, Elke Eggenhofer

{"title":"Glimpse into the role of Kupffer cells in a spheroid model of metabolic dysfunction-associated steatohepatitis (MASH).","authors":"Niklas Bogovic, Maria Drießlein, Marie Keller, Hans J Schlitt, Edward K Geissler, Lydia Schneider, Philipp Kreiner, Henrik Junger, Elke Eggenhofer","doi":"10.1242/bio.062552","DOIUrl":"10.1242/bio.062552","url":null,"abstract":"Metabolic dysfunction-associated steatotic liver disease (MASLD) and its progressive form, metabolic dysfunction-associated steatohepatitis (MASH), are characterized by lipid accumulation, inflammation, and fibrosis. Existing preclinical models often fail to capture multicellular interactions that shape disease progression. In this study, we established a human multicellular 3D liver spheroid model comprising HepaRG hepatocytes, hepatic stellate cells, liver sinusoidal endothelial cells, and Kupffer cells. Spheroids were analyzed for lipid accumulation, cytokine secretion, hepatocellular injury, and extracellular matrix remodeling using immunofluorescence, qPCR, ELISA, and LDH release assays. The model exhibited multicellular spatial organization resembling the native liver microarchitecture. Lipid accumulation was most pronounced under MASLD conditions, whereas progression to MASH was associated with stronger inflammatory and fibrosis-related changes. TNF-α showed the clearest Kupffer cell-associated increase, whereas IL-6 changes were less consistent and likely reflected the broader inflammatory response of a multicellular system. LDH release increased with disease severity and was most pronounced in MASH without evidence of a robust Kupffer cell-specific effect. Fibrotic remodeling was reflected by increased expression of fibronectin, collagen III, and procollagen I. Together, these findings establish a human multicellular in vitro platform that recapitulates the key disease-relevant features of MASLD/MASH under controlled conditions.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13312915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147962280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interferon signaling is required for early neutrophil recruitment after zebrafish heart injury. 斑马鱼心脏损伤后早期中性粒细胞募集需要干扰素信号。

IF 1.8 4区生物学

Biology Open Pub Date : 2026-06-15 DOI: 10.1242/bio.062374

Alexis V Schmid, James A Gagnon

{"title":"Interferon signaling is required for early neutrophil recruitment after zebrafish heart injury.","authors":"Alexis V Schmid, James A Gagnon","doi":"10.1242/bio.062374","DOIUrl":"10.1242/bio.062374","url":null,"abstract":"Heart injury triggers a robust cellular response in zebrafish, characterized by neovascularization, immune cell activation, and the infiltration of proliferative cardiomyocytes that collectively lead to scarless regeneration. Upon injury, damage-associated molecular patterns are released by dying cells and injured extracellular matrix. These molecules bind to pattern recognition receptors on various cell types, promoting inflammation and immune cell recruitment by upregulating chemokines and pro-inflammatory cytokines. We previously identified the activation of injury-induced interferon signaling as a distinguishing feature between the regenerative zebrafish and non-regenerative medaka. Here, we establish interferon-Φ1 (IFNφ1) as the primary driver of interferon signaling after zebrafish heart injury. IFNφ1 expression is induced hours after injury and directs interferon-stimulated gene expression, which peaks at 3 days post-injury. This response is lost in ifnphi1 mutants, which disrupt IFNφ1 expression. ifnphi1 mutants have reduced neutrophil recruitment to the injured myocardium, while macrophages and neovascularization are unaffected. By studying later stages of regeneration, we find that ifnphi1 mutant hearts have a modest defect in fibrotic tissue resolution. Collectively, these findings uncover a critical early signaling cascade during the inflammatory response to heart injury and provide new insights into the mechanisms that choreograph zebrafish heart regeneration.","PeriodicalId":9216,"journal":{"name":"Biology Open","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13312930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0