BMC Plant Biology最新文献

{"title":"Genome-wide identification and expression profiling of WRKY gene family in grain Amaranth (Amaranthus hypochondriacus L.) under salinity and drought stresses.","authors":"Akshay Singh, Avantika Maurya, Rajat Gupta, Parampara Joshi, S Rajkumar, Amit Kumar Singh, Rakesh Bhardwaj, G P Singh, Rakesh Singh","doi":"10.1186/s12870-025-06270-x","DOIUrl":"10.1186/s12870-025-06270-x","url":null,"abstract":"<p><strong>Background: </strong>The WRKY gene family plays a significant role in plant growth, development, and responses to biotic and abiotic stresses. However, the role of the WRKY gene family has not been reported in Amaranthus hypochondriacus. This study presents a comprehensive genome-wide analysis of the WRKY gene family in grain amaranth (A. hypochondriacus L.), a resilient crop known for its high nutritional value and adaptability to challenging environments.</p><p><strong>Results: </strong>In this study, 55 WRKY genes (AhyWRKY1-55) were identified in A. hypochondriacus and distributed unevenly across 16 scaffolds. Of these, 50 contained conserved WRKY domains and were classified into three main groups. Group II was further divided into five subgroups (IIa-IIe) based on phylogenetic analysis, with each clade being well supported by conserved motifs. Additionally, the gene structure analysis revealed variations in exon-intron organization. In contrast, motif analysis showed the presence of conserved domains that were similar within the group but differed between groups, suggesting their functional diversity. Cis-acting elements related to plant growth and development and light, hormones, and stress responses were identified. Synteny analysis revealed that 34 (61.8%) of the genes originated from tandem duplication, indicating the role of tandem duplication in the expansion of the A. hypochondriacus WRKY gene family. Protein-protein interaction analysis suggested that AhyWRKY3, AhyWRKY27, AhyWRKY28, AhyWRKY36, and AhyWRKY52 were hub genes involved in the complex protein interaction network. Using in silico and real-time quantitative PCR, expression analysis revealed tissue- and condition-specific expression patterns of AhyWRKY genes. Notably, under drought stress, AhyWRKY39, AhyWRKY40, AhyWRKY54, and AhyWRKY01 showed increased expression, while under salt stress, AhyWRKY40, AhyWRKY54, AhyWRKY39, AhyWRKY49, and AhyWRKY8 were upregulated at 30 days, suggesting that these genes may play key role in response to salinity stress.</p><p><strong>Conclusions: </strong>The present study provides valuable insights into the organization and evolutionary patterns of the WRKY gene family in amaranth. It also identifies putative candidate WRKY genes that may play a role in conferring drought and salt tolerance. Overall, this study lays a foundation for further functional validation of these WRKY candidate genes, facilitating their exploitation in the amaranth genetic improvement programs to develop stress-resilient varieties.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"265"},"PeriodicalIF":4.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiological and transcriptomic analyses reveal that cell wall biosynthesis and expansion play an important role in the regulation of plant height in alfalfa<sup />.","authors":"Fang Jing, Shang-Li Shi, Wen-Juan Kang, Bei Wu, Bao-Fu Lu, Jian Guan","doi":"10.1186/s12870-025-06172-y","DOIUrl":"10.1186/s12870-025-06172-y","url":null,"abstract":"<p><strong>Background: </strong>Alfalfa (Medicago sativa L.) is a high-quality, high-protein forage, and the improvement and breeding of key traits are important for enhancing the productivity of alfalfa. Plant height is an important trait that affects crop yield, and its regulatory network mechanism has been widely reported in model plants, however, there are fewer studies on the developmental regulatory of plant height in alfalfa.</p><p><strong>Results: </strong>In this study, we screened tall (WL525HQ) and short (WL343HQ) alfalfa materials through field experiments and analyzed the regulatory mechanism of plant height based on the multidimensional joint analysis of phenotype, cell, physiology, and molecular biology. The results showed that internode length was an important factor determining plant height in alfalfa, and cell size affected the internode elongation to a certain extent, whereas cell size was limited by cell wall. Moreover, changes in cell wall components play an important role in cell wall expansion, especially lignin synthesis. Transcriptome analysis showed that the high expression of hydrolase activity in T1 (initiation growth period) facilitates the expansion of the cell wall, the significant enrichment of the cellular modification process in T3 (rapid growth period) increases the cell size, and the synthesis of cell wall structural constituents and plant-type cell wall organization in T5 (growth stabilization) further improves and modifies the cell wall structure. Differential genes involved in cell wall biosynthesis and expansion were mainly enriched in cellulose synthesis, pectin cleavage, lignin formation, expansion protein (EXP), and xyloglucan endotransglycosidase (XTH).</p><p><strong>Conclusions: </strong>These findings elucidated the plant height regulation mechanisms throughout the alfalfa plant and provided a theoretical basis for the generation of ideal alfalfa plant height germplasm.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"267"},"PeriodicalIF":4.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological resistance of Dendrocalamus brandisii culms to alcohol stress during living bamboo wine production process.","authors":"Yingdan Yan, Yufang Wu, Chongsheng Zhao, Fangwei Zhu, Jiaxin Liu, Sushuang Wang, Shuguang Wang","doi":"10.1186/s12870-025-06255-w","DOIUrl":"10.1186/s12870-025-06255-w","url":null,"abstract":"<p><strong>Background: </strong>Currently, living bamboo wine has gained immense popularity in China. However, the effects of alcohol on the physiological processes of bamboo during the production of living bamboo wine have not yet been investigated.</p><p><strong>Methods: </strong>To investigate the resistance of bamboo culms to the alcohol-induced stress, we conducted a comprehensive analysis of various factors, including changes in cell wall chemical components, antioxidant defense systems, endogenous plant hormones and transcriptomics, both before and after the alcohol injection.</p><p><strong>Results: </strong>The results showed that alcohol injection caused more (8.33 ± 8.33%) significant damage to the 1-year-old culms (22.22 ± 4.81%) than that to those aged 2 and 3 years. Bamboos aged 2 years exhibited a superior capacity to rapidly activate their physiological defenses against alcohol stress. The upregulation of key genes in the jasmonic acid biosynthesis pathway, including LOX2S, AOS, MFP2, and OPR, along with an increased accumulation of jasmonic acid, were pivotal in conferring alcohol resistance to bamboo culms post-injection. Additionally, alcohol induced the upregulation of critical genes involved in the lignin and cellulose deposition pathways, such as PAL, 4CL, CAD, SUS and UGP, which lead to enhanced lignin and cellulose deposition, thereby reinforcing the bamboo's resistance to alcohol stress. The SOD enzyme and the AsA-GSH cycle played a crucial role in the bamboo's defense against alcohol-induced stress.</p><p><strong>Conclusions: </strong>The 2-year-old bamboo culms emerged as the optimal choice for living bamboo wine production, due to their exceptional tolerance to alcohol stress. This could provide theoretical guidance for the commercial production of the living bamboo wine and the administration of bamboo forests.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"263"},"PeriodicalIF":4.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis offers deep insights into the Increased Grain Length 1 (IGL1) regulation of grain length.","authors":"Liran Sang, Ending Xu, Yan Liu, Tiange Hu, Mengqi Yang, Jiayu Niu, Chong Lu, Yi Zhou, Yifei Sun, Zhaoyu Zhai, Dina Abdulmajid, Peijiang Zhang, Qianqian Wang, Honggui La, Yu Zou","doi":"10.1186/s12870-025-06279-2","DOIUrl":"10.1186/s12870-025-06279-2","url":null,"abstract":"<p><strong>Background: </strong>Although great progress has been made in recent years in identifying novel genes or natural alleles for rice yield improvement, the molecular mechanisms of how these genes/natural alleles regulate yield-associated traits, such as grain length and 1000-grain weight, remain largely unclear. An in-depth understanding of the roles of these genes/natural alleles in controlling yield traits become a necessity to ultimately increase rice yield via novel molecular techniques, such as gene editing.</p><p><strong>Results: </strong>In this study, the roles of IGL1, which was previously identified through a map-based cloning approach, in the regulation of grain length were investigated by overexpressing and knocking out it in the Nipponbare genetic background. Overexpression and knockout of IGL1 (the resulting transgenic lines were hereafter designated IGL1-OE and IGL1-CR lines, respectively) led to elongation and shortening of grains, respectively. To further elucidate the molecular mechanisms behind the IGL1 action, young panicles from IGL1-OE and IGL1-CR lines were subjected to mRNA sequencing. The results showed that both overexpression and knockout of IGL1 all resulted in a large number of upregulated and downregulated differentially expression genes (DEGs) relative to wild-type NPB control lines. A total of 984 DEGs overlapped between upregulated DEGs from IGL1-OE and downregulated DEGs from IGL1-CR; 1146 DEGs were common to downregulated DEGs from IGL1-OE and upregulated DEGs from IGL1-CR. GO term and KEGG pathway analysis revealed that IGL1-upregulated DEGs were associated with extracellular region, protein ubiquitination, cell-wall modification, BR signaling, cell cycle, etc.; by comparison, the IGL1-downregulated DEGs were connected with extracellular region, response to wounding, flavonoid biosynthesis, jasmonic-acid signaling, glucose/sucrose metabolism, etc. Some phytohormone-associated genes (like OsYUCCA4, OsPIN10b, OsBAK1, and OsDLT), TF genes (like OsMADS1 and OsGASR9), grain length-regulating genes (like An-1, GS9, OsIQD14, and TGW2) showed significant upregulation or downregulation in IGL1-OE or IGL1-CR.</p><p><strong>Conclusion: </strong>Our result clearly demonstrated that IGL1 is an important regulator of grain length, and has profound impacts on genome-wide gene expression, suggesting that it may work together with certain TFs. Overexpression or knockout of IGL1 appears to cause complex expression changes of genes associated with phytohormones, TFs, grain length-regulating factors, which ultimately brings about the grain elongation.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"264"},"PeriodicalIF":4.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Microalgae for bioremediation: advances, challenges, and public perception on genetic engineering.","authors":"Victoria Calatrava, David Gonzalez-Ballester, Alexandra Dubini","doi":"10.1186/s12870-025-06277-4","DOIUrl":"10.1186/s12870-025-06277-4","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"261"},"PeriodicalIF":4.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cysteine-rich transmembrane module peptide GhCYSTM9 is involved in cold stress response.","authors":"Xiao Cai, Cunjing Liu, Liyuan Tang, Sujun Zhang, Xinghe Li, Haitao Wang, Jianhong Zhang","doi":"10.1186/s12870-025-06271-w","DOIUrl":"10.1186/s12870-025-06271-w","url":null,"abstract":"<p><strong>Background: </strong>Cysteine-rich transmembrane module (CYSTM) peptides, which are widely distributed and highly conserved in eukaryotes, are largely involved in stress response and defence. However, the role of cotton CYSTM genes in the stress response has not been functionally characterized.</p><p><strong>Results: </strong>In this study, we identified GhCYSTM9 as a cold stress-responsive CYSTM member from upland cotton. Compared with that in control cotton plants, GhCYSTM9 silencing in cotton resulted in reduced tolerance under cold stress, accompanied by higher MDA contents and lower proline contents and SOD activities in leaves. Overexpressing GhCYTMS9 in Arabidopsis significantly increased the seed germination rates and root elongation at the germination stage. Compared with wild-type seedlings, GhCYSTM9-overexpressing seedlings presented lower MDA contents and greater proline contents in leaves under cold stress. Transcriptome analysis of transgenic Arabidopsis revealed that GhCYSTM9 may contribute to the cold response by regulating oxidative stress-related genes to mediate ROS levels. Yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that GhCYSTM9 interacted with the light-harvesting chlorophyll a/b-binding protein GhLHBC2A1.</p><p><strong>Conclusions: </strong>Overall, our results revealed a positive role of GhCYSTM9 in cold stress defence and suggested candidate genes for the genetic breeding of cold defence.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"262"},"PeriodicalIF":4.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Correlation between aboveground vegetation composition and soil seed bank of Raudhat desert habitat: a case study of Raudhat Alkhafs, Saudi Arabia.","authors":"Asma A Al-Huqail, Hanan F Al-Harbi, Abdullah M Alowaifeer, Mohamed A El-Sheikh, Abdulaziz M Assaeed, Turki S Alsaleem, Hazem S Kassem, Omar M Azab, Basharat A Dar, Jahangir A Malik, Ahmed M Abd-ElGawad","doi":"10.1186/s12870-025-06261-y","DOIUrl":"10.1186/s12870-025-06261-y","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"260"},"PeriodicalIF":4.3,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11863945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing the path from conservation to expansion evolutionary insights into NLR genes in oleaceae.","authors":"Saba Parvez, Maryam Asif, Alizay Ahmad, Iqra Javaid, Muhammad Zaman Rasheed, Romana Iftikhar, Nada H Aljarba, Rabia Zafar, Aqsa Sarwar, Rao Sohail Ahmed Khan, Saad Serfraz","doi":"10.1186/s12870-025-06233-2","DOIUrl":"10.1186/s12870-025-06233-2","url":null,"abstract":"<p><p>The Oleaceae family, encompassing key genera such as Fraxinus (ash trees), Olea (olives), Jasminum (jasmine), Syringa (lilac), and Forsythia, plays a crucial ecological and economic role. Despite their importance, the evolutionary dynamics and immune system adaptations of their NLR (Nucleotide binding leucine-rich repeats) gene family remain largely unexplored. This study employs high-throughput comparative genomics to investigate NLR gene evolution across the Oleaceae family. The genus Fraxinus is widely distributed across both the New and Old Worlds, with 23 distinct species analyzed in this study. Our results reveal a predominant strategy of gene conservation in the evolution of the NLR gene family across these species. Geographical adaptation has played a significant role, particularly in Old World ash tree species, which exhibit dynamic patterns of gene expansion and contraction within the last 50 million years. Notably, genes acquired from an ancient whole genome duplication event (~ 35 Mya) have been retained across Fraxinus lineages. In contrast, the genus Olea (olives) has undergone extensive gene expansion driven by recent duplications and significant birth of novel NLR gene families. These differences in NLR gene evolution likely enhance Olea's ability to recognize diverse pathogens through recent expansions, while Fraxinus maintains specialized immune responses through conserved genes, with potential trade-offs in pathogen adaptation and energy efficiency. In terms of NLR distribution, all species of the Oleaceae family show an enhanced pseudogenization of TIR-NLRs and expansion in CCG10-NLR. However, the comparative RNA-seq expression analysis in olive suggests that partial NLR genes, despite their incomplete structure, have significant expression and may play important roles in plant immune responses. This study provides a comprehensive analysis of NLR gene evolution within the Oleaceae family, offering insights into the adaptive mechanisms of immune response evolution across diverse genera.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"259"},"PeriodicalIF":4.3,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11863892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the molecular mechanism underlying proline metabolic and catabolic pathways and some morpho-physiological traits of tobacco (Nicotiana tabacum L.) plants under arsenic stress.","authors":"Nader Adamipour, Farzad Nazari, Ayoub Molaahmad Nalousi, Jaime A Teixeira da Silva","doi":"10.1186/s12870-025-06262-x","DOIUrl":"10.1186/s12870-025-06262-x","url":null,"abstract":"<p><strong>Background: </strong>In recent decades, arsenic (As) toxicity has emerged as a significant challenge in many countries. It not only reduces the growth and performance of plants, but also poses a threat to human health. The synthesis of compatible solutes, particularly proline, is a mechanism plants utilize to cope with stress. Investigating the metabolic pathways of proline would deepen our understanding for future molecular breeding or genetic engineering efforts. Therefore, the aim of this study was to explore the metabolic and catabolic pathways of proline, as well as the morpho-physiological traits of tobacco, under As stress.</p><p><strong>Results: </strong>The results revealed a significant decrease in morphological traits and photosynthetic efficiency, chlorophyll content, and total soluble protein content with increasing As concentration. The results also showed that proline content, total soluble carbohydrates, hydrogen peroxide, and malondialdehyde, as well as the activity of two antioxidant enzymes, superoxide dismutase and ascorbate peroxidase, increased with increasing As concentration. At 10 mg As Kg^-1 soil, the expression of Δ¹-pyrroline-carboxylate synthetase (P5CS) and P5C reductase (P5CR) genes was not different from the control, but their expression increased significantly at 20 and 40 mg As Kg^-1 soil. At 10 mg As Kg^-1 soil, the expression of proline dehydrogenase (PDH) and P5C dehydrogenase (P5CDH) genes decreased sharply compared to the control but remained unchanged at 20 and 40 mg As Kg^-1 soil. At 10 and 20 mg As Kg^-1 soil, expression of the ornithine δ-aminotransferase (OAT) gene was unchanged compared to the control, but at 40 mg As Kg^-1 soil, it increased sharply.</p><p><strong>Conclusion: </strong>The results showed that the accumulation of proline at the lowest (10 mg As Kg^-1 soil) tested As concentration was due to a decrease in the expression of proline catabolic genes (PDH and P5CDH), while the genes involved in proline synthesis did not play a role. At 20 mg As Kg^-1 soil, proline accumulation was caused by the increased expression of genes (P5CS and P5CR) involved in the glutamate pathway of proline synthesis. Additionally, at the highest concentration of arsenic (40 mg As Kg^-1 soil), the OAT gene, which is active in the ornithine pathway, was also involved in proline synthesis, along with the P5CS and P5CR genes.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"258"},"PeriodicalIF":4.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143498997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative proteome and transcriptome analyses suggest the regulation of starch and sucrose metabolism and rubber biosynthesis pathways in the recovery of tapping panel dryness in rubber tree.","authors":"Kun Yuan, Chengtian Feng, Qiguang He, Yiyu Hu, Hui Liu, Zhenhui Wang","doi":"10.1186/s12870-025-06254-x","DOIUrl":"10.1186/s12870-025-06254-x","url":null,"abstract":"<p><strong>Background: </strong>Tapping panel dryness (TPD) in rubber tree has become the most severe restricting factor of natural rubber production. To date, there is no effective measures to prevent and control TPD. Previous studies primarily focused on analyzing the molecular mechanism underlying TPD occurrence. However, there is no research on the molecular mechanism of TPD recovery.</p><p><strong>Results: </strong>In this study, the TPD trees were recovered by treatment with TPD rehabilitation nutrient agents that could promote the recovery of latex flow on the tapping panel of TPD trees. The genes and proteins involved in TPD recovery were first identified by employing integrated transcriptomics and proteomics analyses. In total, 2029 differentially expressed genes (DEGs) and 951 differentially expressed proteins (DEPs) were detected in the bark of recovery trees compared to that of TPD trees. Among them, 19 DEPs and 11 DEGs were found to be involved in the starch and sucrose metabolism pathway, suggesting their important roles in regulating the syntheses of sucrose and D-glucose, which were the key precursors of natural rubber biosynthesis. Furthermore, 16 DEPs and 15 DEGs were identified in the rubber biosynthesis pathway. Interestingly, almost all the DEPs and DEGs related to rubber biosynthesis exhibited significantly up-regulated expressions in the recovery trees, indicating that latex biosynthesis were probably markedly enhanced during TPD recovery.</p><p><strong>Conclusions: </strong>These results provide new insights into the molecular mechanisms underlying TPD recovery, as well as excellent supplements to the mechanisms of TPD occurrence, which will contribute to the development of more effective agents for the prevention and treatment of TPD in the future.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"250"},"PeriodicalIF":4.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}