BMC Plant Biology最新文献

{"title":"Identification of candidate genes and proteins for tasseling stage drought tolerance through integrated transcriptomic and proteomic analysis approach in maize.","authors":"Songtao Liu, Hanbo Shi, Linan Yan, Chao Jiang, Haichao Zhao, Haibo Lu, Haoyang Li, Shuo Wang, Zhihong Huang","doi":"10.1186/s12870-025-07264-5","DOIUrl":"https://doi.org/10.1186/s12870-025-07264-5","url":null,"abstract":"<p><p>Drought stress, particularly at the tasseling stage, is the most devastating abiotic factor and major contributor to yield reduction in maize (Zea mays L.). Despite recent scientific advances in deciphering maize drought stress responses, the overall picture of key genes and proteins regulating maize tasseling drought tolerance remains less understood. In this study, we conducted comparative physiological, transcriptomic and proteomic analyses to monitor the changes in the leaf tissues of two contrasting maize hybrid cultivars exposed to drought stress at the tasseling stage. We identified 1701 differentially expressed genes (DEGs) in RNA-sequence runs and 424 differentially expressed proteins (DEPs) from an iTRAQ-based analysis. Mapman analysis revealed several regulatory processes influenced by drought conditions, including signal transduction, cell-wall remodeling, cellular redox homeostasis and hormone metabolism that were observed at both mRNA and protein levels. However, transcription factor regulation and secondary metabolism were specifically identified at the transcript level, whereas photosynthesis was uniquely identified to be affected by drought stress at the protein level. Meanwhile, a weak correlation between DEGs and DEPs was observed, indicating the drought response of maize at tasseling stage is largely regulated post-transcriptionally. Furthermore, comparative physiological analysis and qRT-PCR results substantiated the trancriptomic and proteomic findings. Additionally, we screened ZmPOD, ZmRAV1, ZmTPP and performed phenotypical and physiological characterizations of transgenic Arabidopsis thaliana (Arabidopsis) lines and wild-type. Resultantly, the transgenic Arabidopsis lines exhibited stronger tolerance to drought than the WT. This functional verification reinforces the reliability of our omics-based candidate gene selection. Overall, our research provides insights on the drought-responsive genes and pathways mediating maize drought tolerance at the tasseling stage.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1344"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of plant growth regulators and fe₃o₄ nanoparticles on in vitro organogenesis and bioactive compound production in hypericum perforatum.","authors":"Khawlah Mahmood Al Nooh, Aysar Mohammed Salim, Waad S Faizy, Soheila Aghaei Dargiri, Heidar Meftahizade","doi":"10.1186/s12870-025-07409-6","DOIUrl":"https://doi.org/10.1186/s12870-025-07409-6","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1335"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agronomic parameters and drought tolerance indices of bread wheat genotypes as influenced by well-watered and water deficit conditions.","authors":"Md Abu Hena Sorwar Jahan, Md Golam Azam, Mohammed Mohi-Ud-Din, Md Motiar Rohman, Ferdousi Begum, Md Hafizul Haque Khan, Istiak Ahmed, Md Noor Alam Chowdhury, Md Shamsul Alam, Md Robiul Alam, Mohammad Kamrul Hasan, Tasnim Zerin Khan, Ahmed Gaber, Viliam Bárek, Marian Brestic, Akbar Hossain","doi":"10.1186/s12870-025-07355-3","DOIUrl":"https://doi.org/10.1186/s12870-025-07355-3","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;A primary threat to food security stems from the expanding global population and climate change, which have increased the frequency of droughts. Owing to shifting climatic conditions, abiotic stresses such as severe drought are intensifying, reducing wheat productivity. This study aimed to evaluate the response of elite drought-tolerant wheat genotypes to water deficit stress by analysing agronomic and physio-biochemical traits, with the goal of identifying promising genotypes for breeding.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Twenty wheat genotypes sourced from various national and international drought-tolerant nurseries, including a benchmark variety, were tested under water deficit and well-watered conditions over two consecutive years. The data collected included agronomic traits such as plant height (PH), days to heading (DH), days to anthesis (DA), days to physiological maturity (DPM), canopy temperature, SPAD values at different growth stages, intercepted photosynthetically active radiation above the canopy (IPARAC) and on the ground (IPAR OG), yield stability index (YSI), stress tolerance index (STI), stress index (SI), leaf area index (LAI), spike length (SL), grains per spike (GPS), 1000-grain weight (TSW), grain yield (GY; t/ha), and biomass yield (BY; t/ha).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;To streamline the study, two years of aggregated data were analysed for each parameter. Drought tolerance was assessed based on grain yield, and multitrait genotype‒ideotype distance (MGIDI) indices were employed to select drought-tolerant wheat genotypes. Significant differences were observed among the wheat genotypes across all measured parameters under both conditions. Under normal conditions, correlation analysis revealed that grain yield (GY) and biomass yield (BY) had the strongest positive relationship (r = 0.75**), followed by TSW, LAI, GPS, SL, PH, DPM, and DA. In contrast, under water deficit stress, BY exhibited a notable correlation with plant height (PH) (r = 0.42). Under both irrigated and water deficit stress situations, GY had positive and substantial correlations with PH, DA, DPM, GPS, SL, the STI, and the YSI. Two of the ten main components (PCs) accounted for 52.3% and 50.4% of the overall variation under water deficit and well-watered conditions, respectively. Additionally, the genotypes were separated into three clusters via a cluster heatmap, and the most tolerant genotypes (E38, E40, E41, E35, and E33) were found to be in cluster 3, which revealed their genetic relatedness. Genotypes E9 and E29 were found to be sensitive to water deficit, whereas genotypes E40, E38, and E35 were drought tolerant, according to tolerance indices.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Plant breeders may find the MGIDI useful for selecting genotypes on the basis of a variety of characteristics because it is a straightforward and robust selection method. Among the 20 wheat genotypes, the most stable and productive were E38,","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1342"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and ecological response of Cinnamomum camphora under drought stress.","authors":"Taoyuan Wang, Demei Xia, Ting Li","doi":"10.1186/s12870-025-07282-3","DOIUrl":"https://doi.org/10.1186/s12870-025-07282-3","url":null,"abstract":"<p><p>The study focused on one-year-old Cinnamomum camphora seedlings and set up three stress environments: drought, waterlogging, and drought rehydration. The physiological and ecological responses of Cinnamomum camphora under different stress conditions were comprehensively analyzed using methods such as biomass measurement, gas exchange parameters (including net photosynthetic rate, stomatal conductance, transpiration rate), stable carbon isotope ratios, and analysis of non structural carbohydrate content. The results showed that drought stress significantly reduced the total biomass of Cinnamomum camphora (23.57 ± 0.56 g, compared to the control group of 40.84 ± 0.61 g), especially the leaf biomass (4.30 ± 0.45 g), which was 59.28% lower than the control group. The root biomass of the waterlogging stress group was 8.86 ± 1.15 g, which was 38.51% lower than the control group. Stable carbon isotope analysis showed that under drought stress, the δ 13 C value of Cinnamomum camphora significantly increased (-26.7‰), indicating that plants improve water use efficiency by closing stomata; The waterlogging stress reduced the δ 13 C value (-29.1‰), indicating limited photosynthetic efficiency. The rehydration experiment showed that the leaf water potential quickly recovered after rehydration, and the photosynthetic parameters (such as photosynthetic rate and stomatal conductance) gradually increased, demonstrating good drought tolerance. Compared with previous studies on single stress or a single organ, this study analyzed the effects of drought, waterlogging, and rehydration stress on the biomass of different organs in Cinnamomum camphora, revealing the resource allocation strategies and physiological adaptation mechanisms of Cinnamomum camphora under different stresses. This study provides a scientific basis for the adaptive management of Cinnamomum camphora in ecological restoration and urban greening.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1333"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A LjBAK1-associated E3 ubiquitin ligase, LjPUB7, negatively regulates early symbiosis by targeting NFRs.","authors":"Yong Feng, Zhongming Zou, Chao Liu, Dandan Zhang, Yanan Wang, Yi Ma, Yangrong Cao, Ping Wu","doi":"10.1186/s12870-025-07265-4","DOIUrl":"https://doi.org/10.1186/s12870-025-07265-4","url":null,"abstract":"<p><p>Nod factor receptors (NFRs) are essential for initiating symbiotic signaling in legumes, mediating rhizobial infection and nodule development. Tight regulation of NFR levels is crucial to prevent inappropriate immune responses and maintain cellular homeostasis. Co-expression of LjNFR1 and LjNFR5 triggers cell death in Nicotiana benthamiana, which is specifically inhibited by LjBAK1-mediated ubiquitination and subsequent degradation, suggesting the existence of a LjBAK1-E3 ligase complex for NFR protein turnover. Further analysis identified LjPUB7, a plant U-box E3 ubiquitin ligase in Lotus japonicus, as a regulator of early symbiotic interactions. LjPUB7 interacts with both LjBAK1 and NFRs, and directly ubiquitinates NFRs. Loss-of-function Ljpub7 mutants display increased infection thread formation, enhanced nodule development, and elevated expression of early nodulation genes. These findings reveal that LjPUB7 negatively regulates early rhizobial infection by targeting NFR1 and NFR5 for ubiquitination and degradation, thereby providing insights into the fine-tuned control of symbiotic signaling in legumes.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1334"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent transcriptional reprogramming and lipid-associated defense under salt stress in mung bean (Vigna radiata).","authors":"Zhi-Wei Wang, Guan Li, Min Liu, Xing Cheng, Lei-Lei Li, Ru-Zhi Li, Ru-Mei Tian, Song Hou, Jiu-Yan Zhao, Yong-Yi Yang, Kun Xie, Xue Chen, Dong Wang, Longxin Wang, Lian-He Zhang, Kai-Hua Jia, Na-Na Li","doi":"10.1186/s12870-025-07281-4","DOIUrl":"https://doi.org/10.1186/s12870-025-07281-4","url":null,"abstract":"<p><strong>Background: </strong>Salt stress severely limits crop productivity worldwide. However, in mung bean (Vigna radiata), an important legume for agriculture and nutrition, the transcriptional mechanisms underlying dose-dependent salt adaptation remain largely unexplored.</p><p><strong>Results: </strong>We combined physiological assays, transcriptomic profiling, and a dose-adapted time-ordered gene co-expression network (TO-GCN) analysis to dissect the regulatory responses of mung bean under a gradient of salt stress concentrations. Salt exposure increased reactive oxygen species and osmolyte accumulation, accompanied by transcriptional reprogramming that intensified with higher salinity. Network analysis identified eight hierarchically organized regulatory layers corresponding to distinct salt stress stages, revealing coordinated activation of ethylene and jasmonic acid signaling pathways. Notably, lipid transfer proteins (VrLTPs) were predominantly induced under high-salt conditions, co-expressed with key transcription factors such as WRKY, MYB, and NAC, and likely function as central regulators of late-stage stress adaptation.</p><p><strong>Conclusion: </strong>These findings reveal a multi-tiered regulatory framework-captured through a dose-responsive TO-GCN approach-integrating hormonal signaling and lipid-mediated defense mechanisms. The identification of VrLTPs as salt-induced transcriptional hubs highlights their critical role in mung bean's adaptive strategy under high salinity.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1343"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal dynamics and optimal dose effects of biochar on soil properties, cotton growth, and bacterial community assembly in saline-alkali soils.","authors":"Yuting Wang, Guangli Tian, Qingqing Zhao, Dongwei Li, Shuai He","doi":"10.1186/s12870-025-07415-8","DOIUrl":"https://doi.org/10.1186/s12870-025-07415-8","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1337"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molybdenum enhances soybean growth and seed quality by modulating osmoregulation, ion homeostasis, and antioxidant activity under saline-alkaline conditions.","authors":"Tayyaba Zulfiqar, Shakeel Ahmad, Jianrong Shao, Haichang Yang, Jian Wei, Fenghua Zhang","doi":"10.1186/s12870-025-07304-0","DOIUrl":"https://doi.org/10.1186/s12870-025-07304-0","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1336"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field resistance to Orobanche crenata in pea (Pisum sativum L.): beyond strigolactones.","authors":"Francesco Arcieri, Gaetano Giudice, Marzia Guerriero, Chiara Delvento, Mario Schilder, Angelica Giancaspro, Pasquale Luca Curci, Catherine Rameau, Luigi Ricciardi, Concetta Lotti, Harro Bouwmeester, Imran Haider, Stefano Pavan","doi":"10.1186/s12870-025-07296-x","DOIUrl":"https://doi.org/10.1186/s12870-025-07296-x","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1340"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Artemisia argyi and Stevia rebaudiana substrate composition on the nutritional quality, yield and mycelial growth of L. edodes addressing future food challenges.","authors":"Hafiz Umair Asdullah, Yue Xu, Asad Abbas, Muhammad Ahmad Hassan, Shoukat Sajad, Muhammad Rafiq, Dongliang Wang, Yougen Chen","doi":"10.1186/s12870-025-07340-w","DOIUrl":"https://doi.org/10.1186/s12870-025-07340-w","url":null,"abstract":"<p><p>Shiitake mushroom (Lentinula edodes), the second-largest edible fungus globally, boasts high nutritional value and medicinal benefits. The increasing planting scale has led to a shortage of traditional wood chips. Using agricultural waste as a substitute substrate can solve the resource shortage and environmental problems in China's mushroom production industry. An experimental study on Shiitake mushroom strain 868 explored the effects of various cultivation substrates through stages like spawn production, synthetic log preparation, and incubation to fructification, aiming to provide a theoretical basis for mushroom production. Optimal solid and liquid media for biomass production were identified, with potato sucrose agar (PSA) and potato sucrose broth (PSB) being most conducive. Temperature and pH effects revealed the highest radial mycelial growth at 24 °C and pH 5.0 and 7.0. Wormwood (A. argyi) and stevia (S. rebaudiana) were tested as substrates. The addition of wormwood at 60% concentration yielded the highest fruiting body yield and nutritional content, except for carbohydrate and protein content. While stevia substrate composition with a 45% concentration produced the highest yield and nutritional content, with the exception of carbohydrate and protein content. A. argyi and S. rebaudiana substrates effectively enhance the yield and nutritional quality of L. edodes, offering sustainable alternatives to traditional sawdust. This study demonstrates that media type, environmental conditions, and substrate formulation significantly affect the growth, yield, and nutritional composition of Lentinula edodes strain 868. Optimal results were achieved with PSA or MEA media, 24 °C, pH 5.0-6.0, and substrates containing moderate levels of wormwood or stevia.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1338"},"PeriodicalIF":4.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}