BMC Plant Biology最新文献

{"title":"Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences","authors":"Yue Zhang, Meifang Song, Deying Tang, Xianjing Li, Niaojiao Xu, Haitao Li, Lu Qu, Yunqiang Wang, Cuiyun Yin, Lixia Zhang, Zhonglian Zhang","doi":"10.1186/s12870-024-05383-z","DOIUrl":"https://doi.org/10.1186/s12870-024-05383-z","url":null,"abstract":"Lasianthus species are widely used in traditional Chinese folk medicine with high medicinal value. However, source materials and herbarium specimens are often misidentified due to morphological characteristics and commonly used DNA barcode fragments are not sufficient for accurately identifying Lasianthus species. To improve the molecular methods for distinguishing among Lasianthus species, we report the complete chloroplast (CP) genomes of Lasianthus attenuatus, Lasianthus henryi, Lasianthus hookeri, Lasianthus sikkimensis, obtained via high-throughput Illumina sequencing. These showed CP genomes size of 160164-160246 bp and a typical quadripartite structure, including a large single-copy region (86675–86848 bp), a small single-copy region (17177–17326 bp), and a pair of inverted repeats (28089–28135 bp). As a whole, the gene order, GC content and IR/SC boundary structure were remarkably similar among of the four Lasianthus CP genomes, the partial gene length and IR, LSC and SSC regions length are still different. The average GC content of the CP genomes was 36.71–36.75%, and a total of 129 genes were detected, including 83 different protein-coding genes, 8 different rRNA genes and 38 different tRNA genes. Furthermore, we compared our 4 complete CP genomes data with publicly available CP genome data from six other Lasianthus species, and we initially screened eleven highly variable region fragments were initially screened. We then evaluated the identification efficiency of eleven highly variable region fragments and 5 regular barcode fragments. Ultimately, we found that the optimal combination fragment' ITS2 + psaI-ycf4' could authenticated the Lasianthus species well. Additionally, the results of genome comparison of Rubiaceae species showed that the coding region is more conservative than the non-coding region, and the ycf1 gene shows the most significant variation. Finally, 49 species of CP genome sequences belonging to 16 genera of the Rubiaceae family were used to construct phylogenetic trees. Our research is the first to analyze the chloroplast genomes of four species of Lasianthus in detail and we ultimately determined that the combination fragment' ITS2 + psaI-ycf4' is the optimal barcode combination for identifying the genus of Lasianthus. Meanwhile, we gathered the available CP genome sequences from the Rubiaceae and used them to construct the most comprehensive phylogenetic tree for the Rubiaceae family. These investigations provide an important reference point for further studies in the species identification, genetic diversity, and phylogenetic analyses of Rubiaceae species.","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"27 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the complete mitochondrial genome of the rice bean (Vigna umbellata).","authors":"Yuqing Wu, Kai Zhang, Boyang Zhang, Yuqian Li, Guiming Liu, Zhen Liang, Jiewei Zhang","doi":"10.1186/s12870-024-05963-z","DOIUrl":"https://doi.org/10.1186/s12870-024-05963-z","url":null,"abstract":"<p><strong>Background: </strong>Rice bean (Vigna umbellata), an underrated legume crop, demonstrates strong adaptability to poor soil fertility and has significant potential to enhance global food security. It is valuable both as a vegetable and fodder crop due to its high protein content, essential fatty acids, and micronutrients. Despite the sequencing of a high-quality genome of rice bean, its mitochondrial genome (mitogenome) sequence has not yet been reported.</p><p><strong>Results: </strong>For the first time, the rice bean mitogenome was assembled and annotated using PacBio HiFi sequencing and Geseq software. The mitogenome is a circular molecule with a length of 404,493 bp, containing 32 protein-coding genes, 17 tRNAs, and 3 rRNAs. Codon usage and sequence repeats were also determined. Six gene migration events from the chloroplast to the mitogenome were detected in rice bean. A phylogenetic analysis, including the rice bean mitogenome and 25 other taxa (23 of which are Fabales species), clarified the evolutionary and taxonomic status of rice bean. Additionally, a collinearity analysis of seven Fabales mitogenomes revealed high structural variability. In total, 473 RNA editing sites in protein-coding genes were identified.</p><p><strong>Conclusions: </strong>This study presents the first sequencing, assembly, annotation, and analysis of the rice bean mitogenome, providing valuable background information for understanding the evolution of this species. These findings lay the groundwork for future genetic studies and molecular breeding efforts aimed at improving rice bean.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1239"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of microRNAs and lncRNAs expression profiles reveals regulatory modules during adventitious shoot induction in Moringa oleifera Lam.","authors":"Endian Yang, Mengxia Zheng, Limei Zhang, Xiaoyang Chen, Junjie Zhang","doi":"10.1186/s12870-024-05983-9","DOIUrl":"https://doi.org/10.1186/s12870-024-05983-9","url":null,"abstract":"<p><strong>Background: </strong>Embryogenic callus (EC) has strong regenerative potential, useful for propagation and genetic transformation. miRNAs have been confirmed to play key regulatory roles in EC regeneration across various plants. However, challenges in EC induction have hindered the breeding of drumstick (Moringa oleifera Lam.), a tree with significant commercial potential. Understanding the regulatory networks of miRNAs-lncRNAs during EC formation in drumstick is crucial for overcoming these barriers.</p><p><strong>Results: </strong>In this study, three drumstick EC small RNA libraries were sequenced using an Illumina Nova 6000 system. We identified 50 known miRNAs and 233 novel miRNAs. Target prediction and functional analysis showed that these miRNAs are involved in plant hormone signal transduction. Notably, miR319a and miR319b were upregulated throughout the entire process, while miR171 and miR160 were downregulated in the earlier stage but upregulated in the later stage. The expression patterns of 6 miRNAs detected by qRT-PCR were consistent with those observed in RNA-seq. The regulatory relationships between 6 selected highly expressed miRNAs and their target genes generally conformed to a negative regulatory pattern. Furthermore, miR156 and MolncRNA2275 were identified as key regulators in miRNA-mRNA-lncRNA network.</p><p><strong>Conclusions: </strong>In summary, our study provides valuable insights into the molecular mechanisms underlying EC formation and enhances the understanding of the miRNA networks involved in this process.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1237"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on molecular response of alfalfa to low temperature stress based on transcriptomic analysis.","authors":"Hongyu Xu, Zipei Zhang, Qingcui Zhao, Yaqi Gao, Yan Xiang, Jialong Chai, Yuying Li, Xiangyang Hou","doi":"10.1186/s12870-024-05987-5","DOIUrl":"https://doi.org/10.1186/s12870-024-05987-5","url":null,"abstract":"<p><strong>Background: </strong>Alfalfa (Medicago sativa L.) is an important high-quality forage crop. Low temperature is an abiotic stress factor that affects the distribution and productivity of alfalfa. To further understand the molecular response to low temperature, and to identify additional genes and metabolic pathways associated with cold tolerance in alfalfa, in this study we conducted transcriptome sequencing, weighted gene co-expression network analysis, KEGG pathway enrichment analysis, and quantitative real-time PCR validation in alfalfa cultivars subjected to low-temperature treatment.</p><p><strong>Results: </strong>Weighted gene co-expression network analysis revealed that three gene modules were significantly negatively correlated with the semi-lethal temperature for alfalfa. Genes in the three modules were used to construct gene co-expression networks, from which MS.gene46105, MS.gene044087, MS.gene76894, MS.gene44620, MS.gene22005, MS.gene045060, MS.gene31405, and MS.gene74761 were selected as important genes associated with cold tolerance. Quantitative real-time PCR analysis of these eight genes validated the reliability of the transcriptome sequencing data. In addition, further analysis of the genes within the three modules revealed that several transcription factors (AP2/ERF, bZIP, C3H, NAC, and others) and metabolic pathways (N-glycan biosynthesis, citrate cycle, glycolysis/gluconeogenesis, and carbon metabolism, and others) responded well to the low temperature.</p><p><strong>Conclusions: </strong>Three gene modules, eight genes, several transcription factors and multiple metabolic pathways associated with cold tolerance were screened. This results will provide a valuable reference for further clarification of the cold tolerance mechanism and breeding for cold tolerance in alfalfa.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1244"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal fungus and Pseudomonas bacteria affect tomato response to Tuta absoluta (Lepidoptera: Gelechiidae) herbivory.","authors":"Wenyuan Zhao, Ewei Du, Rongchao Luo, Yaping Chen, Zhongxiang Sun, Furong Gui","doi":"10.1186/s12870-024-05952-2","DOIUrl":"https://doi.org/10.1186/s12870-024-05952-2","url":null,"abstract":"<p><p>Tuta absoluta (Lepidoptera: Gelechiidae) is one of the most significant invasive and destructive pests worldwide, causing serious economic losses to the tomato industry. Rhizosphere microorganism, such as arbuscular mycorrhizal fungi (AMF) and Pseudomonas bacteria, can interact with plants individually or collectively to improve plant growth and resistance to pests and disease. However, the effects of AMF, Pseudomonas, and their interactions on plant responses to insect herbivores remain unclear. A pot experiment was conducted to investigate the effects of single/dual inoculation with AMF (Funneliformis mosseae, M) and Pseudomonas putida (P) on the growth and defense of tomato variety Dafen (Solanum lycopersicum L.) in response to infestation by T. absoluta, as well as the growth, development, and enzyme activity of insect. The results showed that M, P, and MP promoted tomato growth by increasing nutrient concentrations, with the growth-promoting effect of dual-inoculation significantly surpassing that of single inoculation. M, P, and MP still improved tomato growth in T. absoluta infestation, with biomass increases of 57.34%, 54.46%, and 255.49%. M, P, and MP significantly increased the defense ability of tomato, with jasmonic acid concentrations increasing by 42.15%, 60.87% and 90.02%, and phenylalanine ammonia-lyase activity increasing by 47.40%, 47.68%, and 59.97%. The inoculation treatments inhibited the growth and development of T. absoluta, reduced its feeding, prolonged its growth and development, decreased egg weight, and increased the activity of protective and detoxifying enzymes. Overall, our results indicated that AMF and bacteria can stimulate each other, positively influence tomato growth and enhance resistance to T. absoluta. These findings indicate the feasibility of AMF and bacteria in combinations as potential biocontrol agents for the management of T. absoluta.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1236"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and expression analysis of TALE superfamily genes explore their key roles in response to abiotic stress in Brassica napus.","authors":"Meili Xie, Xiaojuan Zhang, Kexin Liu, Zhixian Qiao, Xiaohui Cheng","doi":"10.1186/s12870-024-05953-1","DOIUrl":"https://doi.org/10.1186/s12870-024-05953-1","url":null,"abstract":"<p><strong>Background: </strong>The three-amino-acid-loop-extension (TALE) superfamily genes are broadly present in plants and play important roles in plant growth, development, and abiotic stress responses. So far, the TALE family in B.napus have not been systematically studied, especially their potential roles in response to abiotic stress.</p><p><strong>Results: </strong>In this study, we identified 74 TALE family genes distributed on 19 chromosomes in the B. napus genome using bioinformatics methods. Phylogenetic analysis divided the BnTALE superfamily into two subfamilies, the BEL1-like (BLH/BELL homeodomain) and the KNOX (KNOTTED-like homeodomain) subfamilies. Moreover, the KNOX subfamily could be further categorized into three clades (KNOX Class I, KNOX Class II, and KNOX Class III). BnTALE members in the same subclass or branch of the phylogenetic tree generally showed similar gene structures and conserved domain compositions, which may indicate that they have similar biological functions. The BnTALE promoter regions contained many hormone-related elements and stress response elements. Duplication events identification analysis showed that WGD/segmental duplications were the main drivers of amplification during the evolution of TALE genes, and most of the duplicated BnTALE genes underwent purifying selection pressures during evolution. Potential protein interaction network analysis showed that a total of 12,615 proteins might interact with TALE proteins in B. napus. RNA-seq and qRT-PCR analyses showed that the expression of BnTALE was tissue-differentiated and can be induced by abiotic stresses such as dehydration, cold, and NaCl stress. In addition, weighted gene co-expression network analysis (WGCNA) identified four co-expression modules containing the most BnTALE genes, which would be notably related to dehydration and cold stresses.</p><p><strong>Conclusions: </strong>Our study paves the way for future gene functional research of BnTALE and facilitate their applications in the genetic improvement of B. napus in response to abiotic stresses.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1238"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of QTLs for early maturity-related traits based on RIL population of two elite cotton cultivars.","authors":"Xiaoge Wang, Fanjin Kong, Liying Gao, Guifang Shen, Bing Duan, Zongwen Wang, Dongdong Xu, Degang Fan, Yongsheng Deng, Zongfu Han","doi":"10.1186/s12870-024-05947-z","DOIUrl":"https://doi.org/10.1186/s12870-024-05947-z","url":null,"abstract":"<p><strong>Background: </strong>Early-maturity cotton varieties have the potential to be cultivated in a wider geographical area, extending as far north as 46 °N in China, and confer to address the issue of competition for land between grain and cotton by reducing their whole growth period (WGP). Therefore, it is of great importance to develop cotton varieties with comprehensive early maturity and high yield following investigating the regulatory mechanism underlying early maturity and identifying early maturity-related genes.</p><p><strong>Results: </strong>In this study, 'SCRC19' and 'SCRC21', two excellent cultivars with significantly different WGP, along with their recombinant inbred lines (RILs) consisting of 150 individuals were re-sequenced, yielding 4,092,677 high-quality single nucleotide polymorphisms (SNPs) and 794 bin markers across 26 chromosomes. A genetic map spanning 2213.71 cM was constructed using the 794 bin markers. Based on this map, we identified a total of 78 early maturity-related QTLs, including 12 QTLs for WGP, 4 for SSP, 12 for SFP, 3 for FBP, 11 for NFFB, 8 for NFB, 16 for HNFFB and 12 for PH. Six QTL clusters, each containing more than four traits, were identified. One particular QTL cluster, which had the largest number of QTLs, ranged from 108.5 cM to 109 cM on Dt3, and contained 39 genes. Through functional analysis, we highlighted two early maturity-related candidates of GH_D03G1554 and GH_D03G1541, which were annotated as a BEL1-like homeodomain protein 8 and a homeobox-leucine zipper family protein, respectively.</p><p><strong>Conclusions: </strong>We have identified a QTL cluster related to six early maturity-associated traits on Dt3. Through annotation of genes from candidate region, we have identified two candidate genes, GH_D03G1554 and GH_D03G1541, whose expression levels in 'SCRC21' were significantly higher than those in 'SCRC19' at different stages of flower bud development. These candidate genes provide new insights into the study of early-maturity mechanism and offer potential genetic improvement of cotton.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1243"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and characterization of Calcium-Dependent Protein Kinase (CDPK) gene family in autotetraploid cultivated alfalfa (Medicago sativa subsp. sativa) and expression analysis under abiotic stresses.","authors":"Bingcheng Han, Xueming Dong, Congcong Shi, Zhaoming Wang, Jiwei Chen, Pengzhen Li, Wei Yan, Qiang Zhou, Zhipeng Liu, Longfeng Yan","doi":"10.1186/s12870-024-05993-7","DOIUrl":"https://doi.org/10.1186/s12870-024-05993-7","url":null,"abstract":"<p><strong>Background: </strong>Calcium-dependent protein kinases (CDPKs), play multiple roles in plant development, growth and response to bio- or abiotic stresses. Calmodulin-like domains typically contain four EF-hand motifs for Ca²⁺ binding. The CDPK gene family can be divided into four subgroups in Arabidopsis, and it has been identified in many plants, such as rice, tomato, but has not been investigated in alfalfa (Medicago sativa subsp. sativa) yet.</p><p><strong>Results: </strong>In our study, 38 non-redundant MsCDPK genes were identified from the \"XinJiangDaYe\" alfalfa genome. They can be divided into four subgroups which is the same as in Arabidopsis and Medicago truncatula, and there were 15, 12,10 and 1 in CDPK I, II, III and IV, respectively. RNA-seq analysis revealed tissue-specificity of 38 MsCDPK genes. After researching the transcriptome data, we found these 38 MsCDPK members responsive to drought, salt, and cold stress treatments. Further analysis showed that the expression of almost all the MsCDPKs is regulated by abiotic stresses. In addition, we chose MsCDPK03, MsCDPK26, MsCDPK31 and MsCDPK36 for RT-qPCR validation which was from CDPK I-IV subgroups respectively. The result showed that the expression of these four genes was significantly induced by drought, salt and cold treatments. The subcellular location experiment showed that these four proteins were all located in nucleus.</p><p><strong>Conclusion: </strong>In our study, we identified 38 distinct MsCDPK genes within the alfalfa genome, which were classified into four groups. We conducted a comprehensive analysis of various gene features, including physicochemical properties, phylogenetic relationships, exon-intron structures, conserved motifs, chromosomal locations, gene duplication events, cis-regulatory elements, 3D structures, and tissue-specific expression patterns, as well as responses to drought, salt, and cold stresses. These results also provide a solid foundation for further investigations into the functions of MsCDPKs aimed at improving drought tolerance in autotetraploid cultivated alfalfa through genetic engineering.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1241"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing critical mechanisms involved in carbon nanosol-mediated tobacco growth using small RNA and mRNA sequencing in silico approach.","authors":"Xueao Zheng, Chen Wang, Kunlong Zhang, Yalong Xu, Xiaozhan Qu, Peijian Cao, Ting Zhou, Qiansi Chen","doi":"10.1186/s12870-024-05992-8","DOIUrl":"https://doi.org/10.1186/s12870-024-05992-8","url":null,"abstract":"<p><p>Nanomaterials have been shown to promote crop growth, yield and stress resistance. Carbon nanosol (CNS), a type of nanomaterial, is used to regulate tobacco shoot and root growth. However, information about the application of CNS to crop plants, especially tobacco, is still limited. Based on differential expression analysis and trend analysis, several miRNAs (miRN21-Novel-5p-mature, miR319b-Probable-5p-mature, miR160a-c-Known/Probable-5p-mature and miR156c-e-Known-5p-mature/star) and their target genes, including transcription factors (TFs), are likely responsible for the effect of CNS on promoting the growth of tobacco plants. In addition, we characterized nine TFs [Nitab4.5_00001789g0110 (NbbZIP), Nitab4.5_00001176g0010 (NbMYB), Nitab4.5_0001366g0010 (NbNAC), Nitab4.5_00000895g013 (NbMYB), Nitab4.5_0001225g0120 (NbNAC), Nitab4.5_0000202g0230 (NbDof), Nitab4.5_0002241g0010 (NbMYB-related), Nitab4.5_0000410g0060 (NbTCP), and Nitab4.5_0000159g0180 (NbC2H2)] associated with the response of tobacco to CNS according to the differential expression analysis, TF‒gene interaction network analysis and weighted correlation network analysis (WGCNA). Taken together, the findings of our study help understand CNS-mediated growth promotion in tobacco plants. The identification of candidate miRNAs and genes will provide potential support for the use of CNS in tobacco.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1233"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic traits, water use and the yield of maize are influenced by soil water stability.","authors":"Ge Li, Huaiyu Long, Renlian Zhang, Aiguo Xu, Li Niu","doi":"10.1186/s12870-024-05942-4","DOIUrl":"https://doi.org/10.1186/s12870-024-05942-4","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study is to investigate the effect of soil water stability on maize (Zea mays L.) yield, water use, and its photosynthetic physiological mechanisms, and to innovate the relationship between maize and soil water, which currently only considers soil water content and neglects soil water stability.</p><p><strong>Methods: </strong>An organized water experiment was conducted on maize. The effects of stable soil water (SW) at two water content levels were examined, with fluctuating soil water (FW) as a control. The assessed effects included leaf water, chlorophyll, gas exchange, leaf water use efficiency (WUE), stable carbon isotope ratio (δ¹³C), and yield of maize.</p><p><strong>Results: </strong>Soil water stability had a significant effect on maize yield, yet it was slightly smaller than soil water content. Compared with FW, SW increased the maximum net photosynthetic rate, saturated light intensity, stomatal conductance, SPAD, leaf water content, and leaf WUE, and decreased δ¹³C, promoting dry matter assimilation and conversion into grain yield, ultimately increasing yield by 100.8%. Under the same soil water stability, 55% FC versus 75% FC weakened photosynthetic capacity and exacerbated stomatal limitation of maize leaves, making them more susceptible to light inhibition, which decreased photoassimilate accumulation, resulting in a significant decrease in yield. And the δ¹³C under 75% FC conditions decreased by 4.7-7.7% compared with 55% FC.</p><p><strong>Conclusion: </strong>In conclusion, SW exhibits a positive effect on maize leaf water content, photosynthetic carbon assimilation, and grain yields, regardless of soil water content. Compared to FW, SW increased leaf WUE and maize yield by enhancing photosynthesis, and SW has stronger discrimination against ¹³C during photosynthetic CO₂ assimilation, thus decreasing leaf δ¹³C. This study fills a gap in understanding how soil water stability influences maize yield and gas exchange, and provides a fresh perspective on how to improve crop yield and WUE by managing soil water stability.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1235"},"PeriodicalIF":4.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}