BMC Plant Biology最新文献

{"title":"TMT-based quantitative proteomics analysis of defense responses induced by the Bph3 gene following brown planthopper infection in rice.","authors":"Dongjin Qing, Weiwei Chen, Jingcheng Li, Baiyi Lu, Suosheng Huang, Li Chen, Weiyong Zhou, Yinghua Pan, Juan Huang, Hao Wu, Yujing Peng, De Peng, Lei Chen, Yan Zhou, Gaoxing Dai, Guofu Deng","doi":"10.1186/s12870-024-05799-7","DOIUrl":"10.1186/s12870-024-05799-7","url":null,"abstract":"<p><strong>Background: </strong>The brown planthopper (BPH) is an economically significant pest of rice. Bph3 is a key BPH resistance gene. However, the proteomic response of rice to BPH infestation, both in the presence and absence of Bph3, remains largely unexplored.</p><p><strong>Results: </strong>In this study, we employed tandem mass tag labeling in conjunction with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to identify differentially expressed proteins (DEPs) in rice samples. We detected 265 and 125 DEPs via comparison of samples infected with BPH for 2 and 4 days with untreated samples of the BPH-sensitive line R582. For the Bph3 introgression line R373, we identified 29 and 94 DEPs in the same comparisons. Bioinformatic analysis revealed that Bph3 significantly influences the abundance of proteins associated with metabolic pathways, secondary metabolite biosynthesis, microbial metabolism in diverse environments, and phenylpropanoid biosynthesis. Moreover, Bph3 regulates the activity of proteins involved in the calcium signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, and plant hormone signal transduction.</p><p><strong>Conclusions: </strong>Our results indicate that Bph3 enhances the resistance of rice to BPH mainly by inhibiting the down-regulation of proteins associated with metabolic pathways; calcium signaling, the MAPK signaling pathway, and plant hormone signal transduction might also be involved in BPH resistance induced by Bph3.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1092"},"PeriodicalIF":4.3,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of nitrogen treatments on some nutrient concentration and bioactive compounds of broccoli.","authors":"Ercan Ekbic, Gulhan Kose","doi":"10.1186/s12870-024-05823-w","DOIUrl":"10.1186/s12870-024-05823-w","url":null,"abstract":"<p><strong>Background: </strong>Nitrogen fertilization in broccoli is very important for plant growth and productivity. However, it is known that the nitrogen source of the nitrogen fertilizer is also effective on quality parameters. In this study we investigated the effects of different nitrogen sources (ammonium sulphate and calcium nitrate) on bioactive compounds and leaf nutrients of broccoli cultivars (Monet, Italian and Karadede).</p><p><strong>Results: </strong>The highest total phenolic content was 0.24 mg g^- 1 fw in cultivar Monet. Nitrate-based fertilizer treatments provided higher total phenolic (0.28 mg g^- 1 fw) and flavonoid (0.56 mg g¹ fw) contents and FRAP (0.24 mmol g^- 1 fw) values compared to ammonium-based fertilizer treatments. On the other hand, DPPH (0.77 mmol g^- 1 fw) values were higher in the ammonium nitrogen treatments. Among the cultivars, Monet had the highest total phenolics, flavonoids and FRAP values. When leaf nutrient concentrations were analyzed, ammonium sulfate application resulted in higher sulfur (0.86%), zinc (20.72 mg kg^- 1) and manganese (28.80 mg kg^- 1) concentrations compared to calcium nitrate. Calcium nitrate treatment provided the highest calcium (2.09%) and iron (37.50 mg kg^- 1) concentration in Monet cultivar. Phosphorus (0.31-0.35%) and magnesium (0.073-0.084%) concentrations differed among cultivars.</p><p><strong>Conclusions: </strong>In conclusion, the choice of nitrogen source and cultivar in broccoli cultivation plays an important role in the bioactive compound profile and nutrient composition of the broccoli crop. The results provide useful information for the improvement of broccoli quality.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1096"},"PeriodicalIF":4.3,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral properties and molecular docking studies of eco-friendly biosynthesized copper oxide nanoparticles against alfalfa mosaic virus.","authors":"Dalia G Aseel, Mona Rabie, Ali El-Far, Ahmed Abdelkhalek","doi":"10.1186/s12870-024-05802-1","DOIUrl":"10.1186/s12870-024-05802-1","url":null,"abstract":"<p><strong>Background: </strong>Nanotechnology has been recognized as a viable technology for enhancing agriculture, particularly in the plant pathogen management area. Alfalfa mosaic virus (AMV) is a global pathogen that affects many plant species, especially economically valuable crops. Currently, there is less data on the interaction of nanoparticles with phytopathogens, particularly viruses. The current study looked into how copper oxide nanoparticles (CuO-NPs)-mediated Haloxylon salicornicum aqueous extract can fight AMV infections on tobacco plants.</p><p><strong>Results: </strong>Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses showed that CuO-NPs have a spherical and hexagonal structure ranging from 20 to 70 nm in size. Fourier transform infrared spectroscopy (FTIR) analysis showed that the produced CuO-NPs have many functional groups and a lot of secondary plant metabolites. Under greenhouse conditions, the foliar application of CuO-NPs (100 ppm) enhanced tobacco growth and decreased viral symptoms. Treatment with CuO-NPs 48 h before (protective treatment) or 48 h after (curative treatment) AMV infection significantly reduced AMV accumulation levels by 97%. Additionally, the levels of total chlorophyll, phenolic, and flavonoid contents, as well as DPPH, exhibited a significant increase in tobacco leaves 30 days after inoculation in comparison to untreated plants. Moreover, considerable differences in levels of different antioxidant enzymes, including SOD, PPO, POX, and CAT, were also observed. On the other hand, the oxidative stress markers (MDA and H₂O₂) were significantly reduced in CuO-NPs-treated plants compared with non-treated plants. It was also found that the protective treatment increased the expression levels of genes involved in the jasmonic pathway (JERF3 and WRKY1). On the other hand, the curative treatment increased the expression levels of polyphenolic pathway acid (CHI and HQT) and the SA-signaling pathway genes (PR-2 and POD). The study of molecular docking interactions with four AMV target proteins showed that CuO-NPs had high binding energy with the viral replication protein 1a, measured at -3.2 kcal/mol. The binding with these proteins can suppress AMV replication and spread, potentially clarifying the mechanism behind the antiviral effect.</p><p><strong>Conclusions: </strong>The overall analysis results indicate that the curative treatment is more influential and successful than the protective treatment in combating AMV infection. Consequentially, CuO-NPs could potentially be employed in foliar sprays for the effective and environmentally friendly management of plant virus infections.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1089"},"PeriodicalIF":4.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruption of the OsWRKY71 transcription factor gene results in early rice seed germination under normal and cold stress conditions.","authors":"Santiago Bataller, James A Davis, Lingkun Gu, Sophia Baca, Gaelan Chen, Azeem Majid, Anne J Villacastin, Dylan Barth, Mira V Han, Paul J Rushton, Qingxi J Shen","doi":"10.1186/s12870-024-05808-9","DOIUrl":"10.1186/s12870-024-05808-9","url":null,"abstract":"<p><strong>Background: </strong>Early seed germination in crops can confer a competitive advantage against weeds and reduce the time to maturation and harvest. WRKY transcription factors regulate many aspects of plant development including seed dormancy and germination. Both positive and negative regulators of seed germination have been reported in many plants such as rice and Arabidopsis. Using a transient expression system, we previously demonstrated that OsWRKY71 is a negative regulator of gibberellin (GA) signaling in aleurone cells and likely forms a \"repressosome\" complex with other transcriptional repressors. Hence, it has the potential to impact seed germination properties.</p><p><strong>Results: </strong>In this study, we demonstrate that OsWRKY71, a Group IIa WRKY gene, appeared at the same time as seed-bearing plants. Rice mutants lacking OsWRKY71 have seeds and embryos that germinate earlier than wildtype controls. In oswrky71 aleurone layers, α-amylase activity was hypersensitive to stimulation by GA₃ and hyposensitive to inhibition by abscisic acid (ABA). Early germination in oswrky71 intact seeds was also hyposensitive to ABA. Transcriptomic profiling during embryo germination and early post-germination growth demonstrates that OsWRKY71 influences the expression of 9-17% of genes in dry and imbibing embryos. Compared to wildtype embryos, the mutant transcriptomes have large temporal shifts at 4, 8 and 12 h after imbibition (HAI). Importantly, many genes involved in the ABA-dependent inhibition of seed germination were downregulated in oswrky71-1. This mutant also displayed altered expression of multiple ABA receptors (OsPYLs/RCARs) that control ABA signaling and the VP1-SDR4-DOG1L branch of ABA signaling that promotes seed dormancy. Association studies reveal an OsWRKY71-containing quantitative trait locus involved in low-temperature seed germinability, qLTG-2. Indeed, oswrky71 seeds germinated early at 15 °C.</p><p><strong>Conclusions: </strong>Rice Group-IIa WRKY transcription factor OsWRKY71 is a master regulator of germination that influences the expression of 9-17% of genes in dry and imbibing embryos. It is also most likely the primary candidate of low-temperature seed germinability QTL, qLTG-2. We propose that knockouts of OsWRKY71 can generate rice varieties with improved germination properties under normal or low-temperature conditions.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1090"},"PeriodicalIF":4.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of postharvest calcium chloride treatments on decay rate and physicochemical quality properties in strawberry fruit.","authors":"Deniz Eroğul, Muttalip Gundogdu, Fatih Sen, Akgul Tas","doi":"10.1186/s12870-024-05792-0","DOIUrl":"10.1186/s12870-024-05792-0","url":null,"abstract":"<p><strong>Background: </strong>Post-harvest losses cause significant product losses in the world, which leads to food waste. Therefore, it is of great importance for people to have access to sufficient amounts of products by increasing the storage period of fruits with applications such as post-harvest calcium chloride (CaCl₂). In this study, the effect of calcium chloride (CaCl₂) on physical quality parameters and bioactive contents of stored strawberry fruit (Albion cv.) was investigated. Accordingly, strawberries were treated with 2%, 4% and 6% CaCl₂ before storage and stored for 15 days (0 ± 0.5 °C and 90 ± 5% RH). Analyses and measurements were conducted every 5 days during the storage period.Weight loss, decay rate, soluble solids content (SSC), acidity, pH, respiration rate, organic acids (malic, citric, ascorbic, fumaric, oxalic, succinic and tartaric) and phenolic compounds (catechin, chlorogenic, ferulic, gallic, o-coumaric, p-coumaric, protocatechuic, quercetin, rutin and syringic) were analyzed as quality parameters during storage.</p><p><strong>Results: </strong>In the study, in general, the best values were observed in 6% CaCl₂-treated fruits in terms of weight loss, SSC, TA, decay and respiration rates, although they varied according to different storage periods. Similarly, in terms of phenolic compounds, organic acids and vitamin C, 6% CaCl₂-treated fruits had significantly better prevention of quality losses. In addition, the most common phenolic compound of strawberry fruits was gallic acid, followed by chlorogenic acid and catechin, respectively. On the other hand, the predominant organic acid observed in the fruits was malic acid, followed by citric acid, succinic acid and oxalic acid, respectively.</p><p><strong>Conclusions: </strong>In this study, it was observed that CaCl₂ applications more effectively prevented weight loss and decay rate by reducing the respiration rate compared to the control group at the end of the storage period (15th day). It was concluded that, particularly, the 6% CaCl₂ dose can be used as an important treatment to extend storage life by preserving fruit quality and biochemical changes.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1088"},"PeriodicalIF":4.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing population genetic structure and diversity and their driving factors in Phoebe zhennan populations.","authors":"Jian Peng, Jiaxin Xie, Yunjie Gu, Hongying Guo, Shuaiying Zhang, Xin Huang, Xiandan Luo, Jianghong Qian, Minhao Liu, Xueqin Wan, Lianghua Chen, Xiong Huang, Fan Zhang, Fang He, Peng Zhu, Yu Zhong, Hanbo Yang","doi":"10.1186/s12870-024-05810-1","DOIUrl":"10.1186/s12870-024-05810-1","url":null,"abstract":"<p><strong>Background: </strong>Phoebe zhennan, commonly known as \"golden-thread nanmu,\" is one of the most valuable and protected tree species in China. An accurate understanding of the population genetic structure and its environmental factors is of significance for the protection and selection of new P. zhennan varieties.</p><p><strong>Results: </strong>Sixteen nSSR and six cpSSR markers were used to determine the genetic diversity and population structure of P. zhennan and the effect of environmental factors on the genetic structure. The nSSR markers identified a total of 451 number of alleles (Na), while cpSSR markers detected 20 Na. A relative high level of genetic diversity was observed in the P. zhennan population evidenced by high Shannon's information index (I) of 0.671 and 2.294 based on cpSSR and nSSR datasets. The low value of fixation index (F) observed from the nSSR dataset indicated low breeding within the population. The genetic differentiation was mainly detected within populations (only 28% and 13% of the variance being between populations according to the nSSR and cpSSR datasets). Among them, the HNSZX (H = 0.469) and SCSZZ (I = 1.943) populations exhibited the highest level of genetic diversity, while the HNXXT (H = 0.041) and SCLJS (I = 0.943) populations exhibited the lowest level of genetic diversity. The average genetic differentiation coefficient (Fst) and gene flow (Nm) were 0.022-0.128 and 1.698-11.373, respectively, which indicated a moderate level of genetic differentiation and a high level of gene flow. STRUCTURE, neighbor-joining clustering, and principal coordinate analysis divided 543 individuals into two or three categories based on the nSSR or cpSSR datasets. Four temperature, three precipitation, five chemical, five physical, and one soil texture variable showed significant effects on the genetic structure and distribution of P. zhennan populations. Compared to nSSR, the genetic differentiation among populations based on cpSSR datasets conformed to the geographic isolation model, suggesting that geographic and genetic distances should be considered for further genetic conservation and breeding utilization. The importance of in situ conservation units, such as populations with a high level of genetic diversity, more private alleles, and haplotypes (e.g., population SCGTS, SCYFS, and YNYJX), should be emphasized. Additionally, breeding, along with artificially assisted population regeneration and restoration, should also be carefully planned, taking into account climate and soil properties at the same time.</p><p><strong>Conclusions: </strong>In conclusion, this study provided genetic background information for the genetic conservation, management, and utilization of P. zhennan.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1091"},"PeriodicalIF":4.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11572363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing rice breeding for drought tolerance: a comprehensive study of traditional and mutant lines through agronomic performance and drought tolerance indices.","authors":"Mohammad Taher Hallajian, Ali Akbar Ebadi, Mojtaba Kordrostami","doi":"10.1186/s12870-024-05771-5","DOIUrl":"10.1186/s12870-024-05771-5","url":null,"abstract":"<p><strong>Background: </strong>Drought stress is a critical challenge to rice production, necessitating the development of drought-tolerant genotypes. This study aimed to evaluate the drought tolerance of rice genotypes, including traditional parental lines (Hashemi, Khazar, Fajr, and Tarom Mahalli) and their corresponding mutant lines, under normal and drought stress conditions.</p><p><strong>Methods: </strong>Agronomic traits such as plant height, spike number, spike length, seed fertility, and yield were analyzed under both conditions. The performance of these genotypes was further assessed using drought tolerance indices. Statistical methods including cluster analysis and principal component analysis (PCA) were applied to identify the most resilient genotypes.</p><p><strong>Results: </strong>Mutant lines demonstrated superior drought resilience compared to their parental counterparts. Specifically, genotypes like TM-230-VE-7-5-1, TM-B-2-1-E, and HM-250-7-6 exhibited higher yields and better stability of key traits under stress conditions. Cluster analysis and PCA emphasized the strong performance of TM-230-VE-7-5-1, which emerged as the most drought-tolerant genotype, excelling across various drought tolerance indices.</p><p><strong>Conclusions: </strong>The selected mutant lines, particularly TM-230-VE-7-5-1, showed significant potential for breeding programs aimed at improving drought tolerance in rice. These findings have substantial implications for enhancing rice production in drought-prone regions, contributing to sustainable agricultural practices.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1087"},"PeriodicalIF":4.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11572063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of Salicornia europaea L. biomass using a computer vision system to distinguish different salt-tolerant populations.","authors":"S Cárdenas-Pérez, M N Grigore, A Piernik","doi":"10.1186/s12870-024-05743-9","DOIUrl":"10.1186/s12870-024-05743-9","url":null,"abstract":"<p><strong>Background: </strong>Salicornia europaea L. is emerging as a versatile crop halophyte, requiring a low-cost, non-destructive method for salt tolerance classification to aid selective breeding. We propose using a computer vision system (CVS) with multivariate analysis to classify S. europaea based on morphometric and colour traits to predict plant biomass and the salinity in their substrate.</p><p><strong>Results: </strong>A trial and validation set of 96 and 24 plants from 2 populations confirmed the efficacy. CVS and multivariate analysis evaluated the plants by morphometric traits and CIELab colour variability. Through Pearson analysis, the strongest correlations were between biomass fresh weight (FW) vs. projected area (PA) (0.91) and anatomical cross-section (ACS) vs. shoot diameter (Sd) (0.94). The PA and FW correlation retrieved different equation fits between lower and higher salt-tolerant populations (R² = 0.93 for linear and 0.90 for 2nd-degree polynomial), respectively. The higher salt-tolerant reached a maximum biomass PA at 400 mM NaCl, while the lower salt-tolerant produced less under 200 and 400 mM. A second Pearson correlation and PCA described sample variability with 80% reliability using only morphometric-colour parameters. Multivariate discriminant analysis (MDA) demonstrated that the method correctly classifies plants (90%) depending on their salinity level and tolerance, which was validated with 100% effectiveness. Through multiple linear regression, a predictive model successfully estimated biomass production by PA, and a second model predicted the salinity substrate (Sal.s.) where the plants thrive. Plants' Sd and height influenced PA prediction, while Sd and colour difference (ΔE1) influenced Sal.s. Models validation of actual vs. predicted values showed a R² of 0.97 and 0.90 for PA, and 0.95 and 0.97 for Sal.s. for lower and higher salt-tolerant, respectively. This outcome confirms the method as a cost-effective tool for managing S. europaea breeding.</p><p><strong>Conclusions: </strong>The CVS effectively extracted morphological and colour features from S. europaea cultivated at different salinity levels, enabling classification and plant sorting through image and multivariate analysis. Biomass and salinity substrate were accurately predicted by modelling non-destructive parameters. Enhanced by AI, machine learning and smartphone technology, this method shows great potential in ecology, bio-agriculture, and industry.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1086"},"PeriodicalIF":4.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and characterization of bZIP gene family explore the responses of PsebZIP44 and PsebZIP46 in Pseudoroegneria libanotica under drought stress.","authors":"Xunzhe Yang, Xiang Li, Xia Wang, Chen Chen, Dandan Wu, Yiran Cheng, Yi Wang, Lina Sha, Houyang Kang, Songqing Liu, Xing Fan, Yinglong Chen, Yonghong Zhou, Haiqin Zhang","doi":"10.1186/s12870-024-05809-8","DOIUrl":"10.1186/s12870-024-05809-8","url":null,"abstract":"<p><p>The fundamental leucine zipper (bZIP) genes play a crucial role as transcriptional coactivators in plants. Pseudoroegneria libanotica served as the maternal contributor to several species within the tribe Triticeae (Poaceae), exhibiting commendable resilience to various stressors. Consequently, bZIP genes of Pseudoroegneria libanotica response to abiotic stresses was important for further understanding of stress tolerance studies in Triticeae. A total of 108 PsebZIPs were identified in this study, and they were divided into 10 subgroups. PsebZIPs were found to be extensively involved in the biological activities within P. libanotica. The RNA-seq results revealed differential responses of PsebZIPs to drought stress in both aerial and root tissues. PsebZIP44 and PsebZIP46 were regarded as potential genes capable of responding to abiotic stress, and their varying degrees of responsiveness to drought, salinity, heavy metals, heat, and oxidative stress have been demonstrated in yeast. Homologous proteins with intact bZIP gene structures of PsebZIP44 or PsebZIP46 have not been identified in wheat. The current investigation not only provides a fundamental basis for further probing into the biological functions of PsebZIPs within P. libanotica but also reveals previously undiscovered potential genes with the ability to respond to abiotic stress. They could contribute to improving the genetic constitution of Triticeae crops, including wheat.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1085"},"PeriodicalIF":4.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Addressing cadmium stress in rice with potassium-enriched biochar and Bacillus altitudinis rhizobacteria.","authors":"Yonghui Liao, Shoucheng Huang, Misbah Hareem, Muhammad Baqir Hussain, Abdullah A Alarfaj, Sulaiman Ali Alharbi, Saleh Alfarraj","doi":"10.1186/s12870-024-05793-z","DOIUrl":"10.1186/s12870-024-05793-z","url":null,"abstract":"<p><p>Cadmium (Cd) is a potentially harmful metal with significant biological toxicity that adversely affects plant growth and physiological metabolism. Excessive Cd exposure in plants leads to stunted plant growth owing to its negative impact on physiological functions such as photosynthesis, nutrient uptake, and water balance. Potassium-enriched biochar (KBC) and Bacillus altitudinis rhizobacteria (RB) can effectively overcome this problem. Potassium-enriched biochar (KBC) significantly enhances plant growth by improving the soil structure, encouraging water retention, and enhancing microbial activity as a slow-release nutrient. Rhizobacteria promote plant growth by improving root ion transport and nutrient availability while promoting soil health and water conservation through RB production. This study examined the effects of combining RB + KBC as an amendment to rice, both with and without Cd stress. Four treatments (control, KBC, RB, and RB + KBC) were applied using a completely randomized design (CRD) in four replications. The results showed that the combination of RB + KBC increased rice plant height (38.40%), shoot length (53.90%), and root length (12.49%) above the control under Cd stress. Additionally, there were notable improvements in chlorophyll a (15.31%), chlorophyll b (25.01%), and total chlorophyll (19.37%) compared to the control under Cd stress, which also showed the potential of RB + KBC treatment. Moreover, increased N, P, and K concentrations in the roots and shoots confirmed that RB + KBC could improve rice plant growth under Cd stress. Consequently, these findings suggest that RB + KBC is an effective amendment to alleviate Cd stress in rice. Farmers should use RB + KBC to achieve better rice growth under cadmium stress.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1084"},"PeriodicalIF":4.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}