Unveiling transfer RNA modifications of oil palm and their dynamic changes during fruit ripening.

IF 4.3 2区生物学 Q1 PLANT SCIENCES

BMC Plant Biology Pub Date : 2025-03-29 DOI:10.1186/s12870-025-06426-9

Dehai Deng, Yichao Qin, Xiuying Lin, Meng Chu, Daizhu Lv, Huan Lin

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引用次数: 0

Abstract

Background: The oil palm (Elaeis guineensis) is a crucial agricultural commodity, yielding the highest oil output among oil-bearing crops. Despite its significance, productivity challenges persist due to genetic and environmental factors. This study breaks new ground by mapping tRNA modifications in oil palm, exploring their roles during fruit ripening, an area not extensively studied in non-model crops.

Results: Utilizing advanced RNA mass spectrometry techniques, we identified 48 distinct tRNA modifications across 88 sites, alongside 164 genes associated with tRNA modifying enzymes. This comprehensive mapping reveals the decreasing nature of most tRNA modifications during fruit development, except for adenosine 2'-O methylation (Am). It hints at a gradual weakening of protein translation quality control and highlights a unique role for Am during fruit ripening. Additionally, lipidomic analysis tracked 674 lipids in oil palm fruits, indicating a correlation between tRNA modifications and the accumulation of specific lipids.

Conclusions: This study mapped tRNA modifications in oil palm for the first time and showed the diversity of dynamic changes in tRNA modifications as the fruits develop.

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揭示油棕转运RNA修饰及其果实成熟过程中的动态变化。

背景：油棕（Elaeis guineensis）是一种重要的农产品，在油料作物中产量最高。尽管具有重要意义，但由于遗传和环境因素，生产力挑战仍然存在。这项研究开辟了新的领域，通过绘制油棕中的tRNA修饰，探索它们在果实成熟过程中的作用，这一领域在非模式作物中尚未得到广泛研究。结果：利用先进的RNA质谱技术，我们在88个位点鉴定了48种不同的tRNA修饰，以及164个与tRNA修饰酶相关的基因。这一全面的图谱揭示了除腺苷2′-O甲基化（Am）外，果实发育过程中大多数tRNA修饰的减少性质。这暗示了蛋白质翻译质量控制的逐渐减弱，并突出了Am在果实成熟过程中的独特作用。此外，脂质组学分析追踪了油棕果实中的674种脂质，表明tRNA修饰与特定脂质积累之间存在相关性。结论：本研究首次绘制了油棕tRNA修饰图谱，揭示了果实发育过程中tRNA修饰动态变化的多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Plant Biology 生物-植物科学

CiteScore

8.40

自引率

3.80%

发文量

539

审稿时长

3.8 months

期刊介绍： BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.