Glycolysis and signal transduction participate in Lycium barbarum in response to NaCl stress through protein phosphorylation.

Abstract

Background: Lycium barbarum L. possesses great salt tolerance and medicinal values, studying its salt tolerance contribute to variety improvement, as well as the increase in yield and quality.

Results: The study integrated the tandem mass tags (TMT) phosphoproteomics and physiological indexes of L. barbarum exposed to different concentrations of NaCl, with the aim of characterizing salt adaptation characteristics of L. barbarum. The findings indicated that a total of 2189 differentially phosphorylated peptides were identified, functional analysis revealed their involvement in glycolysis, plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signal transduction and other pathways, and that the enzyme activities and substances related to glycolysis and signal transduction underwent significant changes under salt stress.

Conclusion: Salt stress enhanced the glycolysis pathway through protein phosphorylation, and the changes in related enzymes activity accelerated the conversion of intermediate metabolites and energy supply. Salt stress led to the accumulation of abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA) levels, triggering signal transduction events regulated by phosphorylated proteins to improve salt tolerance for L. barbarum in saline environments. The phosphorylation of MAPK signaling pathway-related proteins is triggered by reactive oxygen species (ROS) and ABA as signal molecules to induce the expression of downstream salt stress response factors. This study provides a foundation for further analysis of the molecular regulatory mechanism of protein phosphorylation in L. barbarum for salt stress.