Biomarkers in medicine最新文献

{"title":"Biomarkers for diagnosis of acute ischemic stroke and prediction of post-stroke cognitive impairment: miR-93-3p.","authors":"Zhen Li, Qi Chen, Changxin Dou, Xibo Sun","doi":"10.1080/17520363.2025.2595730","DOIUrl":"10.1080/17520363.2025.2595730","url":null,"abstract":"<p><strong>Aims: </strong>This study aims to validate the potential of miR-93-3p, which is aberrantly expressed in acute ischemic stroke (AIS) patients, as a key biomarker for diagnosing AIS and predicting subsequent cognitive impairment.</p><p><strong>Materials & methods: </strong>The level of miR-93-3p was measured using quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) curves were employed to assess the diagnostic value of miR-93-3p in AIS patients and its predictive value for post-stroke cognitive impairment (PSCI) risk after AIS. Additionally, SH-SY5Y cells subjected to oxygen-glucose deprivation (OGD) were used to explore how miR-93-3p regulates cell viability, inflammation, and oxidative stress. The target genes of miR-93-3p were predicted by integrating the TarBase, TargetScan, and miRDB databases, followed by GO and KEGG pathway enrichment analysis of the candidate genes.</p><p><strong>Results: </strong>miR-93-3p levels were higher in AIS patients, with a more pronounced increase in those with PSCI (<i>p</i> < 0.001). ROC curve analysis confirmed that miR-93-3p has strong diagnostic value for differentiating AIS patients from healthy controls. Moreover, miR-93-3p levels can independently predict PSCI occurrence. The levels of C-reactive protein and homocysteine were positively correlated with the level of miR-93-3p. In miR-93-3p-silenced SH-SY5Y cells, OGD-induced cell damage were reversed. The secretion of inflammatory factors and malondialdehyde (MDA) was reduced, while the intracellular levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were increased. The predicted targets of miR-93-3p were significantly enriched in key pathways implicated in stroke pathophysiology, including ubiquitin-mediated proteolysis, cellular response to decreased oxygen levels, and the Notch signaling pathway.</p><p><strong>Conclusions: </strong>In conclusion, miR-93-3p may serve as a biomarker for AIS diagnosis and PSCI prediction.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":"19 23","pages":"1211-1220"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12758287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145818027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and prognostic value of biomarkers in patients with non-obstructive coronary artery disease: a systematic review and meta-analysis.","authors":"Léa Berbach, Elie Fadel, Brian J Potter, Jessica Forcillo, Christine Pacheco","doi":"10.1080/17520363.2025.2590780","DOIUrl":"10.1080/17520363.2025.2590780","url":null,"abstract":"<p><strong>Background: </strong>Cardiovascular disease (CVD) remains the leading cause of premature mortality worldwide. Among its manifestations, ischemia with non-obstructive coronary arteries (INOCA) and myocardial infarction with non-obstructive coronary arteries (MINOCA) are increasingly recognized and associated with major adverse cardiovascular events. While biomarkers are established tools for diagnosing and predicting outcomes in CVD, their role in INOCA and MINOCA remains unclear. This review summarizes current evidence on cardiovascular biomarkers and their clinical relevance in the context of INOCA and MINOCA.</p><p><strong>Methods: </strong>A systematic review of original studies was conducted using Ovid-MedLine and Embase databases. Eligible studies included adult patients diagnosed with INOCA or MINOCA, with measurements of specific serum biomarkers.</p><p><strong>Results: </strong>Of 1,493 records identified, 53 were included in the quantitative analysis, encompassing 10 biomarkers. Among inflammatory markers, only C-reactive protein was significantly higher in INOCA patients compared to healthy controls. Metabolic, coagulation and endothelial biomarkers showed no differences. Limited data in the MINOCA population precluded comprehensive biomarker analysis.</p><p><strong>Conclusion: </strong>Elevated biomarkers of inflammation in INOCA suggest underlying mechanisms such as oxidative stress, cytokine activation, and immune-mediated microvascular dysfunction. Their diagnostic and prognostic potential in INOCA remains promising but requires further validation in clinical studies.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1161-1180"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145630061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of the preoperative hemoglobin-to-RDW ratio in endometrial cancer: a retrospective cohort study.","authors":"Tung-Ying Wu, Pei-Ru Lin, Chew-Teng Kor, Ying-Lin Hsu, Ming-Ju Chen","doi":"10.1080/17520363.2025.2594395","DOIUrl":"10.1080/17520363.2025.2594395","url":null,"abstract":"<p><strong>Aims: </strong>The hemoglobin-to-red cell distribution width ratio (Hb/RDW) is a prognostic biomarker in various malignancies. However, its prognostic value in endometrial cancer (EC) remains unclear. This study investigated the association between preoperative Hb/RDW ratio and 5-year overall survival (OS) in patients with EC.</p><p><strong>Materials & methods: </strong>This retrospective cohort study included 548 patients with histologically confirmed EC who underwent surgery at a single tertiary center in Taiwan between 2010 and 2021. Patients were stratified on the basis of an Hb/RDW threshold of 1.0, which was derived from receiver operating characteristic curve analysis. Propensity score matching (1:1) and multivariate Cox regression were performed to identify the aforementioned association.</p><p><strong>Results: </strong>Patients with an Hb/RDW ratio of < 1 had significantly poorer 5-year OS than did those with an Hb/RDW ratio of ≥ 1 (adjusted HR: 2.15; 95% CI: 1.19-3.89; <i>p</i> = 0.012). The association between preoperative Hb/RDW ratio and 5-year OS persisted even after propensity score matching (adjusted HR: 2.33; 95% CI: 1.20-4.55; <i>p</i> = 0.013).</p><p><strong>Conclusions: </strong>The present study highlights the preoperative Hb/RDW ratio as a readily available, cost-effective biomarker with independent prognostic value in EC. This ratio may be incorporated into preoperative risk models to optimize clinical decision-making.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1085-1091"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145586209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-153-3p modulates osteogenic differentiation of posterior longitudinal ligament cells via targeting <i>KLF5</i>.","authors":"Liqi Chen, Jie Huang","doi":"10.1080/17520363.2025.2587572","DOIUrl":"10.1080/17520363.2025.2587572","url":null,"abstract":"<p><strong>Background: </strong>The ossification of the posterior longitudinal ligament (OPLL) represents a disabling spinal condition. The specific function of miR-153-3p in the context of OPLL has not been elucidated.</p><p><strong>Aim: </strong>To explore the function of miR-153-3p in OPLL and its regulatory mechanism related to KLF5.</p><p><strong>Methods: </strong>RT-qPCR was used to detect the transcriptional levels of miR-153-3p, osteogenic markers, and inflammatory cytokines in OPLL and normal populations. The interaction between miR-153-3p and <i>KLF5</i> was validated through dual-luciferase reporter assays and RNA immunoprecipitation. Cell transfection experiments were performed to analyze the effects of miR-153-3p and <i>KLF5</i> on osteogenic markers and inflammatory cytokines.</p><p><strong>Results: </strong>There was a significant decrease in miR-153-3p expression in OPLL samples. Osteogenic markers and inflammatory cytokines were significantly upregulated. The negative correlation between miR-153-3p and <i>KLF5</i> was validated. MiR-153-3p was found to inhibit <i>KLF5</i> expression. In ligament fibroblasts (LFC), miR-153-3p mimics uppressed the expression of osteogenic markers and inflammatory cytokines. Conversely, <i>KLF5</i> overexpression reversed these inhibitory effects.</p><p><strong>Conclusion: </strong>MiR-153-3p is downregulated in OPLL and acts as a negative regulator of OPLL progression by directly targeting <i>KLF5</i>, thereby inhibiting LFC osteogenic differentiation.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1065-1074"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145548286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the impact of IMDC score parameters on patients with metastatic clear cell renal cell carcinoma.","authors":"Mert Erciyestepe, Tugce Kubra Gunes, Gulhan Dinc, Kubra Akkaya, Ahmet Emin Ozturk, Okan Aydin, Sermin Dinc Sonusen, Emir Celik, Kayhan Erturk, Muhammed Mustafa Atci","doi":"10.1080/17520363.2025.2589382","DOIUrl":"10.1080/17520363.2025.2589382","url":null,"abstract":"<p><strong>Introduction: </strong>Although the International Metastatic Renal Cell Carcinoma Database Consortium (IMDC) scoring system has been used in metastatic renal cell carcinoma (mRCC) for years, debate continues over its ability to predict survival.</p><p><strong>Materials and methods: </strong>Our study was a single-center retrospective review of medical records of 181 patients diagnosed with mRCC between June 2013 and March 2023.</p><p><strong>Results: </strong>Low serum albumin level (<i>p</i> < 0.001) and high serum uric acid level (<i>p</i> < 0.001) were significantly associated with worse OS. Furthermore, the number of metastatic sites (<i>p</i> = 0.003) had a significantly adverse impact on OS. Although the HGB level of <12 g/dL adversely affected OS (<i>p</i> = 0.042), none of the remaining parameters used for the IMDC scoring had a significant impact on OS.</p><p><strong>Discussion: </strong>Even though the IMDC system scores them equally, we concluded that each parameter might express dissimilarities on survival; some might even lack any significant effect. Furthermore, factors such as serum albumin and uric acid levels, the presence of specific organ metastases, or the number of metastatic sites might affect survival more than the IMDC parameters. Further studies are needed to predict prognosis and develop a treatment plan using an individualized risk score that includes molecular biomarkers, imaging findings, and patient clinical characteristics.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1075-1083"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic utility of procalcitonin for detecting bacteremia in persons who inject drugs.","authors":"Renuka Benara, Archana Angrup, Naresh Sachdeva, Navneet Sharma, Ashok Kumar Pannu","doi":"10.1080/17520363.2025.2590782","DOIUrl":"10.1080/17520363.2025.2590782","url":null,"abstract":"<p><strong>Aim: </strong>Persons who inject drugs (PWID) are prone to bloodstream infections, but diagnosis is often delayed. This study evaluated the utility of admission serum procalcitonin for early detection of bacteremia.</p><p><strong>Methods: </strong>A total of 131 adult male PWID admitted with suspected sepsis were prospectively enrolled.</p><p><strong>Results: </strong>Bacteremia was confirmed in 52 (39.7%) cases, primarily due to <i>Staphylococcus aureus</i> and <i>S</i>. haemolyticus. Infective endocarditis was the leading diagnosis among bacteremic patients. Procalcitonin levels ≥0.51 ng/mL demonstrated high sensitivity (88.5%) and negative predictive value (82.9%), but low specificity (36.7%), resulting in limited diagnostic utility (area under the curve [AUC] 0.613). C-reactive protein (AUC 0.680) and serum albumin (AUC 0.723) outperformed procalcitonin. In-hospital mortality was 35.1%.</p><p><strong>Conclusion: </strong>Admission procalcitonin has limited diagnostic value for detecting bacteremia in PWID.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1107-1115"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145522977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CT-based deep learning radiomics model for predicting HER2 expression and prognosis in non-muscle-invasive bladder cancer.","authors":"Tian Jin, Huanrui Liu, Senlin Li, Haonan Chen, Tenglin Shi, Yue Zhan, Haotian Liu, Xinyuan Li, Xin Gou","doi":"10.1080/17520363.2025.2590778","DOIUrl":"10.1080/17520363.2025.2590778","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to extract radiomics (Rad) and deep learning (DL) features from preoperative CT of patients with Non-Muscle Invasive Bladder Cancer (NMIBC) and develop models incorporating clinical characteristics to assess Human-Epidermal-Growth-Factor-Receptor-2 (HER2) expression status and prognosis in these patients.</p><p><strong>Methods: </strong>From January 2019 to December 2024, 181 patients with NMIBC were retrospectively enrolled in this study. A deep learning radio-clinical signature model (DLCS) was created by integrating DL score, Rad score, and clinicopathologic features to predict HER2 expression in NMIBC and compared with a deep learning model, a radiomic model, and a Clinical model. An additional model was built to predict Recurrence-Free Survival (RFS) in NMIBC patients.</p><p><strong>Results: </strong>181 patients with NMIBC were divided into a training cohort (<i>n</i> = 126) and a test cohort (<i>n</i> = 55). The DLCS model achieved the highest area under the curve (AUC) for HER2 prediction in the test cohort (AUC = 0.894 (95% CI: 0.814-0.974)). The univariate and multivariate Cox regression analyses identified both the DL score and Rad score as independent risk factors for RFS (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>The DLCS model demonstrates good diagnostic performance in predicting HER2 expression, and the prognosis model can stratify the risk of tumor recurrence in patients with NMIBC.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1117-1126"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145586233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum neuregulin -1 (NRG-1) is a potential biomarker for early diagnosis and prediction in refractory schizophrenia.","authors":"Ming Shu, Qiuying Chen","doi":"10.1080/17520363.2025.2590781","DOIUrl":"10.1080/17520363.2025.2590781","url":null,"abstract":"<p><strong>Aims: </strong>This study tends to identify serum biomarkers for early diagnosis of treatment-resistant schizophrenia (TRS) and prediction of clozapine treatment response, given the heterogeneity of schizophrenia and limitations of antipsychotic treatment.</p><p><strong>Patients & methods: </strong>Participants of this prospective cohort study (recruited at Shanghai Mental Health Center; 2020-2023) included 42 TRS patients (TRRIP criteria), 42 first-line antipsychotic responders (age/sex-matched 1:1 to TRS), and 70 age/sex-matched healthy controls. TRS patients were stratified post-clozapine into responders (TRS-R, <i>n</i> = 26) and ultra-treatment-resistant (UTRS, <i>n</i> = 16) subgroups. Symptom severity was assessed using PANSS. Serum neuregulin-1 (NRG-1) levels were quantified via ELISA.</p><p><strong>Results: </strong>Healthy controls showed significantly higher NRG-1 levels than schizophrenia patients (F = 39.76, <i>p</i> < 0.001). TRS patients had lower NRG-1 than treatment responders (<i>p</i> < 0.001). Clozapine responders (TRS-R) exhibited increased NRG-1 post-treatment (<i>t</i> = -3.32, <i>p</i> < 0.001), unlike UTRS patients (<i>t</i> = -0.332, <i>p</i> = 0.745). NRG-1 negatively correlated with symptom severity in both TRS (<i>r</i> = -0.647, <i>p</i> < 0.001) and responders (<i>r</i> = -0.596, <i>p</i> < 0.001). NRG-1 demonstrated diagnostic utility for TRS (AUC = 0.827, 95% CI:0.741-0.914; specificity = 0.786, sensitivity = 0.786).</p><p><strong>Conclusion: </strong>Serum NRG-1 shows significant promise as a potential biomarker for diagnosing TRS and monitoring clozapine treatment response, suggesting involvement in TRS pathophysiology.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1127-1135"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145602384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CTCs-based recurrence model for non-metastatic renal cell carcinoma: integrating machine learning and SHAP interpretation.","authors":"Geng Tian, Fabrice Kayitare, Xiaoyong Chen, Chong Yan, Zihao Li, Zhenlong Wang, Tie Chong, Delai Fu","doi":"10.1080/17520363.2025.2590789","DOIUrl":"10.1080/17520363.2025.2590789","url":null,"abstract":"<p><strong>Background: </strong>Postoperative recurrence remains a significant challenge in renal cell carcinoma (RCC). While circulating tumor cells (CTCs) have emerged as promising prognostic biomarkers, the predictive value of their subtypes (epithelial, hybrid, mesenchymal) and their dynamic changes over time for postoperative recurrence is not yet fully understood. This study aimed to analyze CTCs characteristics and develop a prognostic model for recurrence prediction.</p><p><strong>Methods: </strong>We included 124 patients after RCC resection, collecting serial peripheral blood samples at regular intervals post-surgery to quantify CTCs subtypes using standardized enrichment and identification protocols. We extracted 54 variables, comprising 45 CTC characteristics and 9 clinical/pathological factors. Seven machine learning algorithms were trained to predict recurrence based on these features, with the SHAP (SHapley Additive exPlanations) framework applied to interpret the model.</p><p><strong>Results: </strong>Over a median follow-up of 41 months, 24 patients experienced recurrence, while 100 remained recurrence-free. The Random Forest model demonstrated superior performance, achieving a training AUC of 0.84 and a validation AUC of 0.77. SHAP analysis identified key predictors, including pT stage, tumor size, and changes in mesenchymal and hybrid CTC counts.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1047-1055"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145581875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The rs139226362 (delGATT) variant in the <i>NTS</i> gene alters plasma neurotensin levels in schizophrenia.","authors":"Dilek Pirim, Fatih Atilla Bağcı, Esra Boztepe, Emine Merve Akdağ","doi":"10.1080/17520363.2025.2590777","DOIUrl":"10.1080/17520363.2025.2590777","url":null,"abstract":"<p><strong>Background: </strong>Neurotensin (NTS) is a critical neuropeptide involved in multiple pathways in the central nervous and peripheral systems. We assessed the possible functional relevance of NTS in SCZ pathogenesis by focusing on the rs139226362 (delGATT) located in the 3' untranslated region (UTR) of the NTS.</p><p><strong>Methods: </strong>A total of 88 Turkish individuals with known genotypes for the delGATT allele were included in the study. Differences in NTS mRNA expression and circulating NTS protein levels were evaluated by RT-qPCR and ELISA, respectively. The stability of RNA secondary structures and the binding patterns of RNA-binding protein (RBPs) interactions were evaluated by <i>in silico</i> analysis.</p><p><strong>Results: </strong>Plasma NTS protein levels were significantly higher (<i>p</i> = 0.0003) in SCZ patients carrying the delGATT variant compared to those with wild-type genotype. This genotype-protein association was not observed in healthy controls, indicating a disease-specific effect. Moreover, plasma NTS levels were correlated with PANSS scores (<i>r</i> = -0.290, <i>p</i> = 0.041) and BMI (<i>r</i> = 0.306, <i>p</i> = 0.031) in SCZ patients.</p><p><strong>Conclusions: </strong>These findings suggest that rs139226362 may influence post-transcriptional regulation, with the indel potentially associated with milder SCZ symptoms through altered molecular pathways. Further validation in experimental models may support the development of NTS-based personalized treatment strategies for SCZ management.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1137-1146"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145539008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}