Brain Structure & Function最新文献_第4页

Sex-related variability of white matter tracts in the whole HCP cohort. 整个 HCP 队列中白质束的性别差异。

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-07-16 DOI: 10.1007/s00429-024-02833-0

B Herlin, I Uszynski, M Chauvel, S Dupont, C Poupon

{"title":"Sex-related variability of white matter tracts in the whole HCP cohort.","authors":"B Herlin, I Uszynski, M Chauvel, S Dupont, C Poupon","doi":"10.1007/s00429-024-02833-0","DOIUrl":"10.1007/s00429-024-02833-0","url":null,"abstract":"Behavioral differences between men and women have been studied extensively, as have differences in brain anatomy. However, most studies have focused on differences in gray matter, while white matter has been much less studied. We conducted a comprehensive study of 77 deep white matter tracts to analyze their volumetric and microstructural variability between men and women in the full Human Connectome Project (HCP) cohort of 1065 healthy individuals aged 22-35 years. We found a significant difference in total brain volume between men and women (+ 12.6% in men), consistent with the literature. 16 tracts showed significant volumetric differences between men and women, one of which stood out due to a larger effect size: the corpus callosum genu, which was larger in women (+ 7.3% in women, p = 5.76 × 10-19). In addition, we found several differences in microstructural parameters between men and women, both using standard Diffusion Tensor Imaging (DTI) parameters and more complex microstructural parameters from the Neurite Orientation Dispersion and Density Imaging (NODDI) model, with the tracts showing the greatest differences belonging to motor (cortico-spinal tracts, cortico-cerebellar tracts) or limbic (cingulum, fornix, thalamo-temporal radiations) systems. These microstructural differences may be related to known behavioral differences between the sexes in timed motor performance, aggressiveness/impulsivity, and social cognition.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1713-1735"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI: 10.1007/s00429-024-02807-2

David J Madden, Jenna L Merenstein, Hollie A Mullin, Shivangi Jain, Marc D Rudolph, Jessica R Cohen

{"title":"Age-related differences in resting-state, task-related, and structural brain connectivity: graph theoretical analyses and visual search performance.","authors":"David J Madden, Jenna L Merenstein, Hollie A Mullin, Shivangi Jain, Marc D Rudolph, Jessica R Cohen","doi":"10.1007/s00429-024-02807-2","DOIUrl":"10.1007/s00429-024-02807-2","url":null,"abstract":"Previous magnetic resonance imaging (MRI) research suggests that aging is associated with a decrease in the functional interconnections within and between groups of locally organized brain regions (modules). Further, this age-related decrease in the segregation of modules appears to be more pronounced for a task, relative to a resting state, reflecting the integration of functional modules and attentional allocation necessary to support task performance. Here, using graph-theoretical analyses, we investigated age-related differences in a whole-brain measure of module connectivity, system segregation, for 68 healthy, community-dwelling individuals 18-78 years of age. We obtained resting-state, task-related (visual search), and structural (diffusion-weighted) MRI data. Using a parcellation of modules derived from the participants' resting-state functional MRI data, we demonstrated that the decrease in system segregation from rest to task (i.e., reconfiguration) increased with age, suggesting an age-related increase in the integration of modules required by the attentional demands of visual search. Structural system segregation increased with age, reflecting weaker connectivity both within and between modules. Functional and structural system segregation had qualitatively different influences on age-related decline in visual search performance. Functional system segregation (and reconfiguration) influenced age-related decline in the rate of visual evidence accumulation (drift rate), whereas structural system segregation contributed to age-related slowing of encoding and response processes (nondecision time). The age-related differences in the functional system segregation measures, however, were relatively independent of those associated with structural connectivity.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1533-1559"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pioneers of cortical cytoarchitectonics: the forgotten contribution of Herbert Major. 皮层细胞结构学的先驱：赫伯特-梅杰被遗忘的贡献。

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-07-02 DOI: 10.1007/s00429-024-02825-0

Andrew J Larner, Lazaros C Triarhou

{"title":"Pioneers of cortical cytoarchitectonics: the forgotten contribution of Herbert Major.","authors":"Andrew J Larner, Lazaros C Triarhou","doi":"10.1007/s00429-024-02825-0","DOIUrl":"10.1007/s00429-024-02825-0","url":null,"abstract":"The study of cortical cytoarchitectonics and the histology of the human cerebral cortex was pursued by many investigators in the second half of the nineteenth century, such as Jacob Lockhart Clarke, Theodor Meynert, and Vladimir Betz. Another of these pioneers, whose name has largely been lost to posterity, is considered here: Herbert Coddington Major (1850-1921). Working at the West Riding Asylum in Wakefield, United Kingdom, Major's thesis of 1875 described and illustrated six-layered cortical structure in both non-human primates and man, as well as \"giant nerve cells\" which corresponded to those cells previously described, but not illustrated, by Betz. Further journal publications by Major in 1876 and 1877 confirmed his finding of six cortical strata. However, Major's work was almost entirely neglected by his contemporaries, including his colleague and sometime pupil at the West Riding Asylum, William Bevan-Lewis (1847-1929), who later (1878) reported the presence of both pentalaminar and hexalaminar cortices. Bevan-Lewis's work was also later credited with the first illustration of Betz cells.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1655-1663"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional connectivity associations with anterior cingulate sulcal variability. 与前扣带回变异性的结构和功能连接关系

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-06-20 DOI: 10.1007/s00429-024-02812-5

Luke Harper, Olof Strandberg, Nicola Spotorno, Markus Nilsson, Olof Lindberg, Oskar Hansson, Alexander F Santillo

{"title":"Structural and functional connectivity associations with anterior cingulate sulcal variability.","authors":"Luke Harper, Olof Strandberg, Nicola Spotorno, Markus Nilsson, Olof Lindberg, Oskar Hansson, Alexander F Santillo","doi":"10.1007/s00429-024-02812-5","DOIUrl":"10.1007/s00429-024-02812-5","url":null,"abstract":"Sulcation of the anterior cingulate may be defined by presence of a paracingulate sulcus, a tertiary sulcus developing during the third gestational trimester with implications on cognitive function and disease. In this cross-sectional study we examine task-free resting state functional connectivity and diffusion-weighted tract segmentation data from a cohort of healthy adults (< 60-year-old, n = 129), exploring the impact of ipsilateral paracingulate sulcal presence on structural and functional connectivity. Presence of a left paracingulate sulcus was associated with reduced fractional anisotropy in the left cingulum bundle and the left peri-genual and dorsal bundle segments, suggesting reduced structural organisational coherence in these tracts. This association was not observed in the offsite temporal cingulum bundle segment. Left paracingulate sulcal presence was associated with increased left peri-genual radial diffusivity and tract volume possibly suggesting increased U-fibre density in this region. Greater network dispersity was identified in individuals with an absent left paracingulate sulcus by presence of a significant, predominantly intraregional, frontal component of resting state functional connectivity which was not present in individuals with a present left paracingulate sulcus. Seed-based functional connectivity in pre-defined networks was not associated with paracingulate sulcal presence. These results identify a novel association between sulcation and structural connectivity in a healthy adult population with implications for conditions where this variation is of interest. Presence of a left paracingulate sulcus appears to alter local structural and functional connectivity, possibly as a result of the presence of a local network reliant on short association fibres.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1561-1576"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time to bury the chisel: a continuous dorsal association tract system. 是时候埋下凿子了：一个连续的背侧联结束系统。

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-07-16 DOI: 10.1007/s00429-024-02829-w

Emiel van den Hoven, Marco Reisert, Mariacristina Musso, Volkmar Glauche, Michel Rijntjes, Cornelius Weiller

{"title":"Time to bury the chisel: a continuous dorsal association tract system.","authors":"Emiel van den Hoven, Marco Reisert, Mariacristina Musso, Volkmar Glauche, Michel Rijntjes, Cornelius Weiller","doi":"10.1007/s00429-024-02829-w","DOIUrl":"10.1007/s00429-024-02829-w","url":null,"abstract":"The arcuate fasciculus may be subdivided into a tract directly connecting frontal and temporal lobes and a pair of indirect subtracts in which the fronto-temporal connection is mediated by connections to the inferior parietal lobe. This tripartition has been advanced as an improvement over the centuries-old consensus that the lateral dorsal association fibers form a continuous system with no discernible discrete parts. Moreover, it has been used as the anatomical basis for functional hypotheses regarding linguistic abilities. Ex hypothesi, damage to the indirect subtracts leads to deficits in the repetition of multi-word sequences, whereas damage to the direct subtract leads to deficits in the immediate reproduction of single multisyllabic words. We argue that this partitioning of the dorsal association tract system enjoys no special anatomical status, and the search for the anatomical substrates of linguistic abilities should not be constrained by it. Instead, the merit of any postulated partitioning should primarily be judged on the basis of whether it enlightens or obfuscates our understanding of the behavior of patients in which individual subtracts are damaged.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1527-1532"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inferring occluded projectile motion changes connectivity within a visuo-fronto-parietal network. 推断被遮挡的射弹运动会改变视觉-前额-顶叶网络的连通性。

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-06-25 DOI: 10.1007/s00429-024-02815-2

Gabrielle Aude Zbären, Manu Kapur, Sarah Nadine Meissner, Nicole Wenderoth

{"title":"Inferring occluded projectile motion changes connectivity within a visuo-fronto-parietal network.","authors":"Gabrielle Aude Zbären, Manu Kapur, Sarah Nadine Meissner, Nicole Wenderoth","doi":"10.1007/s00429-024-02815-2","DOIUrl":"10.1007/s00429-024-02815-2","url":null,"abstract":"Anticipating the behaviour of moving objects in the physical environment is essential for a wide range of daily actions. This ability is thought to rely on mental simulations and has been shown to involve frontoparietal and early visual areas. Yet, the connectivity patterns between these regions during intuitive physical inference remain largely unknown. In this study, participants underwent fMRI while performing a task requiring them to infer the parabolic trajectory of an occluded ball falling under Newtonian physics, and a control task. Building on our previous research showing that when solving the physical inference task, early visual areas encode task-specific and perception-like information about the inferred trajectory, the present study aimed to (i) identify regions that are functionally coupled with early visual areas during the physical inference task, and (ii) investigate changes in effective connectivity within this network of regions. We found that early visual areas are functionally connected to a set of parietal and premotor regions when inferring occluded trajectories. Using dynamic causal modelling, we show that predicting occluded trajectories is associated with changes in effective connectivity within a parieto-premotor network, which may drive internally generated early visual activity in a top-down fashion. These findings offer new insights into the interaction between early visual and frontoparietal regions during physical inference, contributing to our understanding of the neural mechanisms underlying the ability to predict physical outcomes.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1605-1615"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data-driven exploratory method investigation on the effect of dyslexia education at brain connectivity in Turkish children: a preliminary study. 以数据为驱动的探索性方法调查阅读障碍教育对土耳其儿童大脑连通性的影响：一项初步研究。

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-07-13 DOI: 10.1007/s00429-024-02820-5

Şerife Gengeç Benli, Semra İçer, Esra Demirci, Zehra Filiz Karaman, Zeynep Ak, İrem Acer, Gizem Rüveyda Sağır, Ebru Aker, Büşra Sertkaya

{"title":"Data-driven exploratory method investigation on the effect of dyslexia education at brain connectivity in Turkish children: a preliminary study.","authors":"Şerife Gengeç Benli, Semra İçer, Esra Demirci, Zehra Filiz Karaman, Zeynep Ak, İrem Acer, Gizem Rüveyda Sağır, Ebru Aker, Büşra Sertkaya","doi":"10.1007/s00429-024-02820-5","DOIUrl":"10.1007/s00429-024-02820-5","url":null,"abstract":"Dyslexia is a specific learning disability that is neurobiological in origin and is characterized by reading and/or spelling problems affecting the development of language-related skills. The aim of this study is to reveal functional markers based on dyslexia by examining the functions of brain regions in resting state and reading tasks and to analyze the effects of special education given during the treatment process of dyslexia. A total of 43 children, aged between 7 and 12, whose native language was Turkish, participated in the study in three groups including those diagnosed with dyslexia for the first time, those receiving special education for dyslexia, and healthy children. Independent component analysis method was employed to analyze functional connectivity variations among three groups both at rest and during the continuous reading task. A whole-brain scanning during task fulfillment and resting states revealed that there were significant differences in the regions including lateral visual, default mode, left frontoparietal, ventral attention, orbitofrontal and lateral motor network. Our results revealed the necessity of adding motor coordination exercises to the training of dyslexic participants and showed that training led to functional connectivity in some brain regions similar to the healthy group. Additionally, our findings confirmed that impulsivity is associated with motor coordination and visuality, and that the dyslexic group has weaknesses in brain connectivity related to these conditions. According to our preliminary results, the differences obtained between children with dyslexia, group of dyslexia with special education and healthy children has revealed the effect of education on brain functions as well as enabling a comprehensive examination of dyslexia.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1697-1712"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atypical lateralization of visuospatial attention can be associated with better or worse performance on line bisection. 视觉空间注意力的非典型侧化可能与线段分割的成绩好坏有关。

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-06-22 DOI: 10.1007/s00429-024-02822-3

Esteban Villar-Rodríguez, Tatiana Davydova, Lidón Marin-Marin, César Avila

{"title":"Atypical lateralization of visuospatial attention can be associated with better or worse performance on line bisection.","authors":"Esteban Villar-Rodríguez, Tatiana Davydova, Lidón Marin-Marin, César Avila","doi":"10.1007/s00429-024-02822-3","DOIUrl":"10.1007/s00429-024-02822-3","url":null,"abstract":"The causal and statistical hypotheses diverge in determining whether the lateralization of language function in one cerebral hemisphere entails the lateralization of visuospatial function in the opposite hemisphere. Additionally, it remains unclear if the atypical segregation of these functions could influence cognitive performance. This study addresses these questions by examining the hemispheric lateralization of visuospatial attention during a line bisection judgement (landmark) task in three groups of healthy non-right-handed individuals with different language production segregations: left (typical), ambilateral (atypical), and right (atypical). Consistent with the causal hypothesis, results indicate that the groups with left and right language lateralization primarily utilize the opposite hemisphere for visuospatial attention. The ambilateral group, however, displays a pattern compatible with an independent segregation, supporting the statistical hypothesis. Behavioral analyses reveal that atypical lateralization of visuospatial attention (non-right) can lead to either better or worse performance during the landmark task, depending on the specific pattern. Bilateral organization is associated with reduced overall accuracy, whereas the left segregation results in improved performance during the most challenging trials. These findings suggest the existence of diverse pathways to lateralization, akin to either the causal or statistical hypothesis, which can result in cognitive advantages or disadvantages.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1577-1590"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141440210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating brain function and structure in the study of the human attentional networks: a functionnectome study. 在人类注意力网络研究中整合大脑功能和结构：功能组研究。

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-07-06 DOI: 10.1007/s00429-024-02824-1

Mar Martín-Signes, Pedro M Paz-Alonso, Michel Thiebaut de Schotten, Ana B Chica

{"title":"Integrating brain function and structure in the study of the human attentional networks: a functionnectome study.","authors":"Mar Martín-Signes, Pedro M Paz-Alonso, Michel Thiebaut de Schotten, Ana B Chica","doi":"10.1007/s00429-024-02824-1","DOIUrl":"10.1007/s00429-024-02824-1","url":null,"abstract":"Attention is a heterogeneous function theoretically divided into different systems. While functional magnetic resonance imaging (fMRI) has extensively characterized their functioning, the role of white matter in cognitive function has gained recent interest due to diffusion-weighted imaging advancements. However, most evidence relies on correlations between white matter properties and behavioral or cognitive measures. This study used a new method that combines the signal from distant voxels of fMRI images using the probability of structural connection given by high-resolution normative tractography. We analyzed three fMRI datasets with a visual perceptual task and three attentional manipulations: phasic alerting, spatial orienting, and executive attention. The phasic alerting network engaged temporal areas and their communication with frontal and parietal regions, with left hemisphere dominance. The orienting network involved bilateral fronto-parietal and midline regions communicating by association tracts and interhemispheric fibers. The executive attention network engaged a broad set of brain regions and white matter tracts connecting them, with a particular involvement of frontal areas and their connections with the rest of the brain. These results partially confirm and extend previous knowledge on the neural substrates of the attentional system, offering a more comprehensive understanding through the integration of structure and function.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1665-1679"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic features of gray matter layers and superficial white matter of the rhesus monkey neocortex: comparison of prefrontal area 46 and occipital area 17. 恒河猴新皮层灰质层和表层白质的蛋白质组特征：前额叶区 46 和枕叶区 17 的比较。

IF 2.7 3区医学

Brain Structure & Function Pub Date : 2024-09-01 Epub Date: 2024-06-28 DOI: 10.1007/s00429-024-02819-y

Paola B Castro-Mendoza, Christina M Weaver, Wayne Chang, Maria Medalla, Kathleen S Rockland, Lisa Lowery, Elizabeth McDonough, Merina Varghese, Patrick R Hof, Dan E Meyer, Jennifer I Luebke

{"title":"Proteomic features of gray matter layers and superficial white matter of the rhesus monkey neocortex: comparison of prefrontal area 46 and occipital area 17.","authors":"Paola B Castro-Mendoza, Christina M Weaver, Wayne Chang, Maria Medalla, Kathleen S Rockland, Lisa Lowery, Elizabeth McDonough, Merina Varghese, Patrick R Hof, Dan E Meyer, Jennifer I Luebke","doi":"10.1007/s00429-024-02819-y","DOIUrl":"10.1007/s00429-024-02819-y","url":null,"abstract":"In this novel large-scale multiplexed immunofluorescence study we comprehensively characterized and compared layer-specific proteomic features within regions of interest of the widely divergent dorsolateral prefrontal cortex (A46) and primary visual cortex (A17) of adult rhesus monkeys. Twenty-eight markers were imaged in rounds of sequential staining, and their spatial distribution precisely quantified within gray matter layers and superficial white matter. Cells were classified as neurons, astrocytes, oligodendrocytes, microglia, or endothelial cells. The distribution of fibers and blood vessels were assessed by quantification of staining intensity across regions of interest. This method revealed multivariate similarities and differences between layers and areas. Protein expression in neurons was the strongest determinant of both laminar and regional differences, whereas protein expression in glia was more important for intra-areal laminar distinctions. Among specific results, we observed a lower glia-to-neuron ratio in A17 than in A46 and the pan-neuronal markers HuD and NeuN were differentially distributed in both brain areas with a lower intensity of NeuN in layers 4 and 5 of A17 compared to A46 and other A17 layers. Astrocytes and oligodendrocytes exhibited distinct marker-specific laminar distributions that differed between regions; notably, there was a high proportion of ALDH1L1-expressing astrocytes and of oligodendrocyte markers in layer 4 of A17. The many nuanced differences in protein expression between layers and regions observed here highlight the need for direct assessment of proteins, in addition to RNA expression, and set the stage for future protein-focused studies of these and other brain regions in normal and pathological conditions.","PeriodicalId":9145,"journal":{"name":"Brain Structure & Function","volume":" ","pages":"1495-1525"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0