Biomedical Research-tokyo最新文献_第7页

Serum cell-free DNA concentration as a possible prognostic marker in newly diagnosed diffuse large B-cell lymphoma. 血清游离DNA浓度作为新诊断弥漫性大b细胞淋巴瘤可能的预后指标。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2022-01-01 DOI: 10.2220/biomedres.43.99

Yuko Shirouchi, Yuko Mishima, Tomoko Takayama, Sayuri Minowa, Yuko Ishihara, Mikako Tamba, Mitsuhito Hirano, Naoki Onda, Kengo Takeuchi, Dai Maruyama

{"title":"Serum cell-free DNA concentration as a possible prognostic marker in newly diagnosed diffuse large B-cell lymphoma.","authors":"Yuko Shirouchi, Yuko Mishima, Tomoko Takayama, Sayuri Minowa, Yuko Ishihara, Mikako Tamba, Mitsuhito Hirano, Naoki Onda, Kengo Takeuchi, Dai Maruyama","doi":"10.2220/biomedres.43.99","DOIUrl":"https://doi.org/10.2220/biomedres.43.99","url":null,"abstract":"Cell-free DNA (cfDNA) is a fragment of DNA circulating in the blood, and its concentration is often elevated in cancer patients. To investigate the relationships between serum cfDNA concentration and clinical characteristics, including prognosis, we measured serum cfDNA concentration in 114 newly diagnosed lymphoma patients. The cfDNA concentrations in diffuse large B cell lymphoma (DLBCL) (62.5 ng/mL) and follicular lymphoma patients (51.6 ng/mL) were significantly elevated compared to healthy individuals (7.5 ng/mL, P < 0.001). In DLBCL, patients with elevated serum cfDNA (> 38.9 ng/mL) at diagnosis had significantly shorter time-to-progression compared to those without (P = 0.033). The addition of cfDNA concentration to the international prognostic index showed improved predictive power for time-to-progression. Moreover, cfDNA added significant prognostic value to other inflammatory markers such as B symptoms and sIL2R. There was a trend towards shorter progression-free survival and overall survival in patients with elevated cfDNA. Furthermore, B symptoms (P = 0.038), bulky masses (P = 0.031), non-GCB subtype (P = 0.012), and serum sIL-2R levels > 2,000 U/mL (P = 0.012) were associated with higher cfDNA levels. Our study showed that serum cfDNA concentration at diagnosis was associated with certain clinicopathological characteristics, and may be predictive of survival outcomes in DLBCL patients.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40429937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Activation of STAT3 (signal transducer and activator of transcription 3) in synovial tissues from the hip joint in the early stage of rapidly destructive coxopathy. 快速破坏性髋关节病早期髋关节滑膜组织中STAT3(信号换能器和转录激活因子3)的激活

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2022-01-01 DOI: 10.2220/biomedres.43.173

Tadashi Yasuda, Shigeo Hara, Shinnosuke Yamashita, Sadaki Mitsuzawa, Yoshihiro Tsukamoto, Hisataka Takeuchi, Satoshi Ota, Eijiro Onishi

{"title":"Activation of STAT3 (signal transducer and activator of transcription 3) in synovial tissues from the hip joint in the early stage of rapidly destructive coxopathy.","authors":"Tadashi Yasuda, Shigeo Hara, Shinnosuke Yamashita, Sadaki Mitsuzawa, Yoshihiro Tsukamoto, Hisataka Takeuchi, Satoshi Ota, Eijiro Onishi","doi":"10.2220/biomedres.43.173","DOIUrl":"https://doi.org/10.2220/biomedres.43.173","url":null,"abstract":"Interleukin-6 signaling activates signal transducer and activator of transcription 3 (STAT3), resulting in matrix metalloproteinase-3 (MMP-3) production. The hip joints with rapidly destructive coxopathy (RDC) show rapid chondrolysis, probably by increased MMP-3. This study aimed to elucidate STAT3 activation in the synovial tissues with joint destruction in the early stage of RDC. Synovial tissues within 7 months from the disease onset were obtained from four RDC patients with femoral head destruction and high serum levels of MMP-3. RDC synovial tissues demonstrated the synovial lining hyperplasia with an increase of CD68-positive macrophages and CD3-positive T lymphocytes. STAT3 activation was found in the synovial tissues by immunohistochemistry using anti-phospho-STAT3 antibody. The majority of phospho-STAT3-positive cells were the synovial lining cells and exhibited negative expression of the macrophage or T cell marker. Treatment with CP690,550, a Janus Kinase inhibitor, resulted in a decrease in phospho-STAT3-positive cells, especially with high intensity, indicating effective suppression of STAT3 activation in RDC synovial tissues. Inhibitory effect of CP690,550 could work through the Janus Kinase/STAT3 axis in the synovial tissues in the early stage of RDC. Thus, STAT3 may be a potential therapeutic target for prevention of joint structural damage in RDC.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33512930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathological examination of Ym1, a chitinase family protein, in Mesocestoides corti-infected mice. 几丁质酶家族蛋白Ym1在中皮甾体皮质感染小鼠中的病理检测。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2022-01-01 DOI: 10.2220/biomedres.43.161

Junko Nio-Kobayashi, Makoto Owhashi, Toshihiko Iwanaga

{"title":"Pathological examination of Ym1, a chitinase family protein, in Mesocestoides corti-infected mice.","authors":"Junko Nio-Kobayashi, Makoto Owhashi, Toshihiko Iwanaga","doi":"10.2220/biomedres.43.161","DOIUrl":"https://doi.org/10.2220/biomedres.43.161","url":null,"abstract":"Mammals express a set of chitinase family proteins, comprising chitinases, which can hydrolyze chitin, and chitinase-like proteins without the chitinase activity but possessing chitin-binding properties. They act as endogenous lectins, regulating various physiological/pathological events. Ym1, originally identified as an eosinophil chemotactic factor or a macrophage-derived protein in parasite-infected mice, is a rodent-specific chitinase-like protein. Ym1 is also purified from eosinophilic crystals formed in the lung and urinary system in various disease models. We previously reported that major cellular sources of murine Ym1 are alveolar macrophages in the lung and neutrophils/monocytes lineage cells of the spleen and bone marrow under normal conditions. We here analyzed the detailed cellular expression of Ym1 in Mesocestoides corti (M. corti)-infected mice. Ym1 was significantly increased in the liver containing the larvae, lung, and peritoneal exudate cells in M. corti-infected mice, where activated macrophages expressed Ym1. Characteristic needle-shaped eosinophilic crystals appeared in the larvae-free lung, and Ym1 was localized to endoplasmic reticulum of activated alveolar macrophages. Moreover, swollen mesothelial cells covering the liver, spleen, and heart expressed Ym1 abundantly. Although the role of Ym1 in parasitic infection remains unclear, our findings focusing on an endogenous lectin may help in better understanding defense mechanism against parasites.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33512964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shotgun proteomic investigation of methyltransferase and methylation profiles in lipopolysaccharide stimulated RAW264.7 murine macrophages. 脂多糖刺激RAW264.7小鼠巨噬细胞中甲基转移酶和甲基化谱的散弹枪蛋白质组学研究。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2022-01-01 DOI: 10.2220/biomedres.43.73

Yumi Aizawa, Masaru Mori, Tsukasa Suzuki, Akihiro Saito, Hirofumi Inoue

{"title":"Shotgun proteomic investigation of methyltransferase and methylation profiles in lipopolysaccharide stimulated RAW264.7 murine macrophages.","authors":"Yumi Aizawa, Masaru Mori, Tsukasa Suzuki, Akihiro Saito, Hirofumi Inoue","doi":"10.2220/biomedres.43.73","DOIUrl":"https://doi.org/10.2220/biomedres.43.73","url":null,"abstract":"Arginine methylation is a common post-translational modification which functions as an epigenetic regulator of transcription and plays a key role in various cell signaling pathways. The methylation of arginine residues is catalyzed by protein arginine methyltransferase (PRMT). However, the expression pattern and underlying mechanism of PRMTs and protein methylation profile in lipopolysaccharide (LPS)-induced innate immune responses are poorly understood. Using a shotgun proteomic approach, we found that LPS stimulation increased arginine and proline metabolism and responses to inflammation and bacterial infections. In comparison, cysteine and methionine metabolism, the pentose phosphate pathway, purine metabolism, and protein methylation factors were also decreased in LPS stimulated murine macrophage cell lines. We revealed that LPS stimulation downregulated PRMT1, PRMT5, and protein arginine methylation profiles in RAW264.7 cells using western blot analysis. Additionally, this phenomenon occurred in parallel with nitric oxide accumulation in LPS-induced macrophages. Using inflammation models, we demonstrate for the first time that LPS stimulation decreases PRMTs, leading to the decreasing of arginine methylation in macrophages.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39998563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

RNA impacts formation of biomolecular condensates in the nucleus. RNA影响细胞核内生物分子凝聚物的形成。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.2220/biomedres.42.153

Saho Matsui, Ryu-Suke Nozawa

引用次数: 2

Effect of UGT2B7 Gene Polymorphism with Clinical Pediatric Epileptic patients on Sodium Valproate Monotherapy 临床儿童癫痫患者UGT2B7基因多态性对丙戊酸钠单药治疗的影响

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.S10-S15

ith Pb, Sachidan, A. Adiga, U. Adiga, V. Shenoy, S. Kumari, P. Shetty, S. Shetty, K. Sharmila

{"title":"Effect of UGT2B7 Gene Polymorphism with Clinical Pediatric Epileptic patients on Sodium Valproate Monotherapy","authors":"ith Pb, Sachidan, A. Adiga, U. Adiga, V. Shenoy, S. Kumari, P. Shetty, S. Shetty, K. Sharmila","doi":"10.35841/0970-938X.S10-S15","DOIUrl":"https://doi.org/10.35841/0970-938X.S10-S15","url":null,"abstract":"Background: Pediatric epilepsy comprises of a chronic neurological disorders characterized by recurrent seizure attack. Sodium valproate is one of the common anti-epileptic drugs used in the treatment. Glucuronide conjugation is the major metabolic pathway of sodium valproate, carried out by the enzyme Uridine 5’-diphospho (UDP) glucuronosyl transferase (UGT) whose genetic polymorphisms may alter clinical outcome. Aim: To find the association between UGT2B7 genetic polymorphism and clinical outcome in terms of efficacy and tolerability in pediatric epileptic patients on sodium valproate monotherapy. Methods and Materials: In this cohort study, 75 pediatric epileptic patients aged 2-18 years receiving sodium valproate monotherapy for past one month were included from Justice K S Hegde Charitable Hospital, Mangalore, India after obtaining informed consent. Genetic polymorphism patterns of UGT2B7 (C161T, A268G, G211T) was evaluated by PCR-RFLP. Clinical outcome was measured in terms of responders and non-responders based on seizure control during the 6 month observation period. Tolerability was measured by estimating the hepatic, renal and other lab parameters. Clinical outcome in different UGT genotypes was compared by Chi square test. P value <0.05 was considered as significant. Results: Out of 75 patients, CC (41.3%), CT (38.7%), TT (20%) pattern was observed in UGT2B7 (C161T) gene, AA(14.7%), AG(42.7%), GG(42.7%) in (A268G) gene and GG(80%), GT(18.7%), TT(1.3%) in (G211T) gene. It was found that there was no statistical difference in clinical outcome with different UGT2B7 genetic polymorphism patterns. Conclusion: We conclude from our study that genetic polymorphism of UGT2B7 doesn’t have any role on the clinical outcome of epilepsy.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75241050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation level of IL-10 and TNF-? in Iraq T1DM patient. IL-10和TNF-?伊拉克的T1DM患者。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.32.3.75-75

Sabreen Ali Mezil, R. Al-Shawk

引用次数: 0

Difficulties and Points of view in Nourishing Directing. 滋补指导的难点与观点。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.32.3.S21-S22

S. Ahmed

引用次数: 0

Targeting of the receptor for advanced glycation end products regulates neutrophil infiltration and extravascular recruitment in mice acute pancreatitis. 靶向晚期糖基化终产物受体调节小鼠急性胰腺炎中性粒细胞浸润和血管外募集。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.S28-S33

M. Merza, T. Faraj, Rawaz Dilzar Tawfiq, Rundk Hwaiz, Harm, Ali Hama, Younis Sadiq Smael

{"title":"Targeting of the receptor for advanced glycation end products regulates neutrophil infiltration and extravascular recruitment in mice acute pancreatitis.","authors":"M. Merza, T. Faraj, Rawaz Dilzar Tawfiq, Rundk Hwaiz, Harm, Ali Hama, Younis Sadiq Smael","doi":"10.35841/0970-938X.S28-S33","DOIUrl":"https://doi.org/10.35841/0970-938X.S28-S33","url":null,"abstract":"Infiltration of leukocytes and pancreatic acinar cell damaging are good indicators of extreme Acute Pancreatitis (AP). The signaling pathways for inflammation and tissue damage of the pancreas not been elucidated yet. In this study, we evaluated the role of targeting of the Receptor for Advanced Glycation End products (RAGE) signaling in AP. Moreover, we investigated the role of signaling RAGE in AP. C57BL/6 mice were injected with a RAGE inhibitor (anti-RAGE) (500 μg/kg) before induction of taurocholate into the pancreatic duct to induce pancreatitis. Treatment anti-RAGE decreased blood amylase concentration, neutrophil recruitment in the pancreas, hemorrhage and edema formation in pancreatitis decreased by taurocholate. Additionally, anti-RAGE administration decreased the MPO activity in the pancreas and lung induced by taurocholate. Intraperitoneal (IP) injection of anti-RAGE significantly decreased concentrations of CXCL2 and IL-6 in the pancreas and plasma respectively in response to challenges of taurocholate. Finally, RAGE inhibition did not have a direct impact on secretagogue-induced trypsinogen activation in pancreatic acinar cells in vitro. Thus, these findings show new signaling pathways in AP and suggest that RAGE targeting may be an efficient way to improve extreme AP.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82053239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of role of pharmacists in managing ailments and ensuring medication safety at Dire Dawa town, East Ethiopia 评估药剂师在埃塞俄比亚东部迪勒达瓦镇管理疾病和确保用药安全方面的作用

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.32.5.97-103

Zeray Deresse, Teshome Sosengo, Eyassu Mathewos

{"title":"Assessment of role of pharmacists in managing ailments and ensuring medication safety at Dire Dawa town, East Ethiopia","authors":"Zeray Deresse, Teshome Sosengo, Eyassu Mathewos","doi":"10.35841/0970-938X.32.5.97-103","DOIUrl":"https://doi.org/10.35841/0970-938X.32.5.97-103","url":null,"abstract":"Background: Community pharmacistsare the most accessible healthprofessionals to the general public and pharmacistsareincreasinglybeingrecognized as a source of professionalhealth-relatedadvice. Objective:Theaimofthestudyis to assessroleofpharmacistsin managingailmentsandensuringmedication safety at Dire Dawatown, East Ethiopiafrom Feb20, 2021 to Mar 20, 2021.. Method: Community pharmacy based cross-sectional study was conducted with interview and structured self-administered questionnaires. All community pharmacists found in Dire Dawa town and willing to participate in the study were included. Data was collected from Feb 20, 2021 to Mar 20, 2021. The data is analyzed and presented using tables. Result: In the current study 50 study participants was included. The 87% of the respondents are graduated with first degree and 13% are graduated with masters. All of the respondents replied that they respond effectively to patient’s disease symptoms in their pharmacy and have responsibility to respond to patient’s symptom. The 67% of the respondents replied that that lack of knowledge and influence of pharmaceutical industry is the main factor for inappropriate symptom management in community pharmacy. Disease symptoms managed by the community pharmacists were cold/flue 37[73%], cough 20[40%], diarrhea 14[27%], and inflammation 10[20%]. The 33[67%] of respondents manage disease symptoms by giving medication, while 33[67%] and 14[27%] manage symptoms by advising the patient to visit doctor and to usehomeremediesrespectively. The 33[67%] of respondentsrepliedthatthey ask if thepatienthaveadverse drug reaction before they dispense medication.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76567299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0