Biomedical Research-tokyo最新文献_第7页

RNA impacts formation of biomolecular condensates in the nucleus. RNA影响细胞核内生物分子凝聚物的形成。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.2220/biomedres.42.153

Saho Matsui, Ryu-Suke Nozawa

引用次数: 2

Effect of UGT2B7 Gene Polymorphism with Clinical Pediatric Epileptic patients on Sodium Valproate Monotherapy 临床儿童癫痫患者UGT2B7基因多态性对丙戊酸钠单药治疗的影响

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.S10-S15

ith Pb, Sachidan, A. Adiga, U. Adiga, V. Shenoy, S. Kumari, P. Shetty, S. Shetty, K. Sharmila

{"title":"Effect of UGT2B7 Gene Polymorphism with Clinical Pediatric Epileptic patients on Sodium Valproate Monotherapy","authors":"ith Pb, Sachidan, A. Adiga, U. Adiga, V. Shenoy, S. Kumari, P. Shetty, S. Shetty, K. Sharmila","doi":"10.35841/0970-938X.S10-S15","DOIUrl":"https://doi.org/10.35841/0970-938X.S10-S15","url":null,"abstract":"Background: Pediatric epilepsy comprises of a chronic neurological disorders characterized by recurrent seizure attack. Sodium valproate is one of the common anti-epileptic drugs used in the treatment. Glucuronide conjugation is the major metabolic pathway of sodium valproate, carried out by the enzyme Uridine 5’-diphospho (UDP) glucuronosyl transferase (UGT) whose genetic polymorphisms may alter clinical outcome. Aim: To find the association between UGT2B7 genetic polymorphism and clinical outcome in terms of efficacy and tolerability in pediatric epileptic patients on sodium valproate monotherapy. Methods and Materials: In this cohort study, 75 pediatric epileptic patients aged 2-18 years receiving sodium valproate monotherapy for past one month were included from Justice K S Hegde Charitable Hospital, Mangalore, India after obtaining informed consent. Genetic polymorphism patterns of UGT2B7 (C161T, A268G, G211T) was evaluated by PCR-RFLP. Clinical outcome was measured in terms of responders and non-responders based on seizure control during the 6 month observation period. Tolerability was measured by estimating the hepatic, renal and other lab parameters. Clinical outcome in different UGT genotypes was compared by Chi square test. P value <0.05 was considered as significant. Results: Out of 75 patients, CC (41.3%), CT (38.7%), TT (20%) pattern was observed in UGT2B7 (C161T) gene, AA(14.7%), AG(42.7%), GG(42.7%) in (A268G) gene and GG(80%), GT(18.7%), TT(1.3%) in (G211T) gene. It was found that there was no statistical difference in clinical outcome with different UGT2B7 genetic polymorphism patterns. Conclusion: We conclude from our study that genetic polymorphism of UGT2B7 doesn’t have any role on the clinical outcome of epilepsy.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75241050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation level of IL-10 and TNF-? in Iraq T1DM patient. IL-10和TNF-?伊拉克的T1DM患者。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.32.3.75-75

Sabreen Ali Mezil, R. Al-Shawk

引用次数: 0

Difficulties and Points of view in Nourishing Directing. 滋补指导的难点与观点。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.32.3.S21-S22

S. Ahmed

引用次数: 0

Targeting of the receptor for advanced glycation end products regulates neutrophil infiltration and extravascular recruitment in mice acute pancreatitis. 靶向晚期糖基化终产物受体调节小鼠急性胰腺炎中性粒细胞浸润和血管外募集。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.S28-S33

M. Merza, T. Faraj, Rawaz Dilzar Tawfiq, Rundk Hwaiz, Harm, Ali Hama, Younis Sadiq Smael

{"title":"Targeting of the receptor for advanced glycation end products regulates neutrophil infiltration and extravascular recruitment in mice acute pancreatitis.","authors":"M. Merza, T. Faraj, Rawaz Dilzar Tawfiq, Rundk Hwaiz, Harm, Ali Hama, Younis Sadiq Smael","doi":"10.35841/0970-938X.S28-S33","DOIUrl":"https://doi.org/10.35841/0970-938X.S28-S33","url":null,"abstract":"Infiltration of leukocytes and pancreatic acinar cell damaging are good indicators of extreme Acute Pancreatitis (AP). The signaling pathways for inflammation and tissue damage of the pancreas not been elucidated yet. In this study, we evaluated the role of targeting of the Receptor for Advanced Glycation End products (RAGE) signaling in AP. Moreover, we investigated the role of signaling RAGE in AP. C57BL/6 mice were injected with a RAGE inhibitor (anti-RAGE) (500 μg/kg) before induction of taurocholate into the pancreatic duct to induce pancreatitis. Treatment anti-RAGE decreased blood amylase concentration, neutrophil recruitment in the pancreas, hemorrhage and edema formation in pancreatitis decreased by taurocholate. Additionally, anti-RAGE administration decreased the MPO activity in the pancreas and lung induced by taurocholate. Intraperitoneal (IP) injection of anti-RAGE significantly decreased concentrations of CXCL2 and IL-6 in the pancreas and plasma respectively in response to challenges of taurocholate. Finally, RAGE inhibition did not have a direct impact on secretagogue-induced trypsinogen activation in pancreatic acinar cells in vitro. Thus, these findings show new signaling pathways in AP and suggest that RAGE targeting may be an efficient way to improve extreme AP.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82053239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of role of pharmacists in managing ailments and ensuring medication safety at Dire Dawa town, East Ethiopia 评估药剂师在埃塞俄比亚东部迪勒达瓦镇管理疾病和确保用药安全方面的作用

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.32.5.97-103

Zeray Deresse, Teshome Sosengo, Eyassu Mathewos

{"title":"Assessment of role of pharmacists in managing ailments and ensuring medication safety at Dire Dawa town, East Ethiopia","authors":"Zeray Deresse, Teshome Sosengo, Eyassu Mathewos","doi":"10.35841/0970-938X.32.5.97-103","DOIUrl":"https://doi.org/10.35841/0970-938X.32.5.97-103","url":null,"abstract":"Background: Community pharmacistsare the most accessible healthprofessionals to the general public and pharmacistsareincreasinglybeingrecognized as a source of professionalhealth-relatedadvice. Objective:Theaimofthestudyis to assessroleofpharmacistsin managingailmentsandensuringmedication safety at Dire Dawatown, East Ethiopiafrom Feb20, 2021 to Mar 20, 2021.. Method: Community pharmacy based cross-sectional study was conducted with interview and structured self-administered questionnaires. All community pharmacists found in Dire Dawa town and willing to participate in the study were included. Data was collected from Feb 20, 2021 to Mar 20, 2021. The data is analyzed and presented using tables. Result: In the current study 50 study participants was included. The 87% of the respondents are graduated with first degree and 13% are graduated with masters. All of the respondents replied that they respond effectively to patient’s disease symptoms in their pharmacy and have responsibility to respond to patient’s symptom. The 67% of the respondents replied that that lack of knowledge and influence of pharmaceutical industry is the main factor for inappropriate symptom management in community pharmacy. Disease symptoms managed by the community pharmacists were cold/flue 37[73%], cough 20[40%], diarrhea 14[27%], and inflammation 10[20%]. The 33[67%] of respondents manage disease symptoms by giving medication, while 33[67%] and 14[27%] manage symptoms by advising the patient to visit doctor and to usehomeremediesrespectively. The 33[67%] of respondentsrepliedthatthey ask if thepatienthaveadverse drug reaction before they dispense medication.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76567299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive Dry Eye detection using Fuzzy c-means Clustering Algorithm 基于模糊c均值聚类算法的无创干眼检测

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.S1-S5

I.Ch, ra, N.Prabhakaran, V.Prabhu, S.Harshavardhan, N.Duraichi

引用次数: 0

Cutaneous angiosarcoma of the scalp: A case report and literature review 头皮皮肤血管肉瘤1例报告及文献复习

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.35841/0970-938X.32.4.79-83

S. Harrak, S. Lemsanes, S. Razine, S. Najem, H. Mrabti, S. Jaba, S. ElMajjaoui, N. Benjaafar, H. Errihani

引用次数: 0

Cellular expression and subcellular localization of diacylglycerol kinase γ in rat brain. 二酰基甘油激酶γ在大鼠脑中的细胞表达和亚细胞定位。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.2220/biomedres.42.33

Yasukazu Hozumi, Tomoyuki Nakano, Kaoru Goto

{"title":"Cellular expression and subcellular localization of diacylglycerol kinase γ in rat brain.","authors":"Yasukazu Hozumi, Tomoyuki Nakano, Kaoru Goto","doi":"10.2220/biomedres.42.33","DOIUrl":"https://doi.org/10.2220/biomedres.42.33","url":null,"abstract":"Gq protein-coupled receptors lead to activation of phospholipase C, which triggers phosphoinositide signaling. Diacylglycerol (DG) is one of the phosphoinositide metabolites and serves as a second messenger. Diacylglycerol kinase (DGK) phosphorylates DG to produce another second messenger phosphatidic acid. Of the DGK family, DGKγ is predominantly expressed in the brain at the mRNA level. Recent studies have shown the expression of DGKγ in vascular endothelial cells and adrenal medullary cells at the protein level, although its detailed cellular expression pattern and subcellular localization in the brain remain to be determined. In the present study, we addressed this point using specific DGKγ antibody. DGKγ was expressed in both projection neurons and interneurons in the cerebral cortex, hippocampal formation, and cerebellum. In cerebellar Purkinje cells, DGKγ was distributed to the soma and dendrites. Fractionation study revealed that DGKγ was enriched in the internal membranes containing the endoplasmic reticulum and Golgi complex. In immunoelectron microscopy, DGKγ was localized throughout the smooth endoplasmic reticulum system. These findings suggest that DGKγ shows unique cellular expression pattern in the brain and distinct subcellular localization different from other DGK isozymes.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25353635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular expression of CD26/dipeptidyl peptidase IV. CD26/二肽基肽酶IV的细胞表达。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2021-01-01 DOI: 10.2220/biomedres.42.229

Toshihiko Iwanaga, Junko Nio-Kobayashi

{"title":"Cellular expression of CD26/dipeptidyl peptidase IV.","authors":"Toshihiko Iwanaga, Junko Nio-Kobayashi","doi":"10.2220/biomedres.42.229","DOIUrl":"https://doi.org/10.2220/biomedres.42.229","url":null,"abstract":"Dipeptidyl peptidase 4 (DPP4), a serine protease expressed on luminal and apical cell membrane, is identical to the lymphocyte cell surface protein CD26. DPP4 rapidly deactivates hormones and cytokines by cleaving their NH2-terminal dipeptides. Its functions are based on membrane digestion and/or binding of bioactive peptides, signal molecules, and extracellular matrix components. The soluble form is also present in body fluids such as serum, urine, semen, and synovial fluid. The extremely broad distribution of CD26/DPP4 indicates its divergent roles depending on cell type and activated conditions. The cellular localization was earlier examined by enzyme histochemistry and subsequently by immunohistochemistry. Although immunohistochemical analyses are higher in specificity and easier to use at electron microscopic levels than enzyme histochemistry, the immunoreaction is considerably affected by the animal species, types of tissue sections, and specificity of antibodies. Understanding of the functional significance and advancement of its clinical use (diagnosis and treatment of diseases) require precise information on the cellular distribution including subcellular localization and pathological changes. This short review summarizes in particular immunohistochemical findings on CD26/DPP4.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39749586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4